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Plant Cell Advance Online Publication
Published on June 29, 2007; 10.1105/tpc.106.045666

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Received July 10, 2006
Returned for revision May 29, 2007
Accepted June 6, 2007

INCREASED SIZE EXCLUSION LIMIT2 Encodes a Putative DEVH Box RNA Helicase Involved in Plasmodesmata Function during Arabidopsis Embryogenesis

Ken Kobayashi 1, Marisa S. Otegui 2, Sujatha Krishnakumar 3, Michael Mindrinos 3, and Patricia Zambryski 1*

1 Department of Plant and Microbial Biology, University of California, Berkeley, California 94720
2 Department of Botany, University of Wisconsin, Madison, Wisconsin 53706-1381
3 Stanford Genome Technology Center, Palo Alto, California 94304

* To whom correspondence should be addressed. E-mail: zambrysk{at}nature.berkeley.edu.

Here, we characterize the Arabidopsis thaliana embryo-defective mutant increased size exclusion limit2 (ise2). In contrast with wild-type embryos, ise2 mutants continue to traffic 10-kD fluorescent dextran in the mid-torpedo stage of development. ise2 embryos contain branched as well as simple plasmodesmata (PD) compared with wild-type embryos, which only contain simple PD. Positional cloning reveals that the ISE2 gene encodes a putative DEVH box RNA helicase that shares sequence homology with RNA helicases involved in RNA degradation pathways in other organisms. ISE2 localizes to granule-like structures in the cytoplasm. These granules increase in number when plant cells are stressed. These features are characteristic of stress granules (SGs) in mammalian cells, suggesting that ISE2 granules represent plant-specific SGs. Genetic data demonstrate that the ISE2 helicase is involved in posttranscriptional gene silencing and the determination of cell fate. These data together suggest that ISE2 function affects PD structure and function through the regulation of RNA metabolism and consequent gene expression.




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