Plant Cell Advance Online Publication
Published on December 28, 2006; 10.1105/tpc.106.046151
OPEN ACCESS ARTICLE
Received August 10, 2006
Returned for revision October 31, 2006
Accepted November 20, 2006
MAIGO2 Is Involved in Exit of Seed Storage Proteins from the Endoplasmic Reticulum in Arabidopsis thaliana
Lixin Li 1, Tomoo Shimada 1, Hideyuki Takahashi 1, Haruko Ueda 1, Yoichiro Fukao 2, Maki Kondo 3, Mikio Nishimura 3, and Ikuko Hara-Nishimura 1*
1 Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
2 Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0101, Japan
3 Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
* To whom correspondence should be addressed. E-mail: ihnishi{at}gr.bot.kyoto-u.ac.jp.
Seed storage proteins are synthesized on the endoplasmic reticulum (ER) as precursors and then transported to protein storage vacuoles, where they are processed into mature forms. Here, we isolated an Arabidopsis thaliana mutant, maigo2 (mag2), that accumulated the precursors of two major storage proteins, 2S albumin and 12S globulin, in dry seeds. mag2 seed cells contained many novel structures, with an electron-dense core that was composed of the precursor forms of 2S albumin. 12S globulins were segregated from 2S albumin and were localized in the matrix region of the structures together with the ER chaperones lumenal binding protein and protein disulfide isomerase, which were more abundant in mag2 seeds. The MAG2 gene was identified as At3g47700, and the MAG2 protein had a RINT-1/TIP20 domain in the C-terminal region. We found that some MAG2 molecules were peripherally associated with the ER membrane. MAG2 had an ability to bind to two ER-localized t-SNAREs (for target-soluble NSF [N-ethylmaleimide-sensitive fusion protein] attachment protein receptor; At Sec20 and At Ufe1). Our findings suggest that MAG2 functions in the transport of storage protein precursors between the ER and Golgi complex in plants.
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