Plant Cell Advance Online Publication
Published on December 15, 2006; 10.1105/tpc.106.046730
OPEN ACCESS ARTICLE
Received August 18, 2006
Returned for revision October 4, 2006
Accepted November 2, 2006
Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21-Mediated Disease Resistance
Yong-Sheng Wang 1, Li-Ya Pi 1, Xiuhua Chen 1, Pranjib K. Chakrabarty 1, Junda Jiang 2, Alfred Lopez De Leon 3, Guo-Zhen Liu 1, Liangcai Li 4, Ulla Benny 1, James Oard 2, Pamela C. Ronald 3, and Wen-Yuan Song 1*
1 Department of Plant Pathology, University of Florida, Gainesville, Florida 32611
2 Department of Agronomy, Louisiana State University, Baton Rouge, Louisiana 70803
3 Department of Plant Pathology, University of California, Davis, California 95616
4 Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27695
* To whom correspondence should be addressed. E-mail: wsong{at}ifas.ufl.edu.
XA21 is a receptor-like kinase protein in rice (Oryza sativa) that confers gene-for-gene resistance to specific races of the causal agent of bacterial blight disease, Xanthomonas oryzae pv oryzae. We identified XA21 binding protein 3 (XB3), an E3 ubiquitin ligase, as a substrate for the XA21 Ser and Thr kinase. The interaction between XB3 and the kinase domain of XA21 has been shown in yeast and in vitro, and the physical association between XB3 and XA21 in vivo has also been confirmed by coimmunoprecipitation assays. XB3 contains an ankyrin repeat domain and a RING finger motif that is sufficient for its interaction with the kinase domain of XA21 and for its E3 ubiquitin ligase activity, respectively. Transgenic plants with reduced expression of the Xb3 gene are compromised in resistance to the avirulent race of X. oryzae pv oryzae. Furthermore, reduced levels of Xb3 lead to decreased levels of the XA21 protein. These results indicate that Xb3 is necessary for full accumulation of the XA21 protein and for Xa21-mediated resistance.
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