Plant Cell Advance Online Publication
Published on May 18, 2007; 10.1105/tpc.106.047621
Received October 10, 2006
Returned for revision April 17, 2007
Accepted April 26, 2007
Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development
David C. Goeres 1, Jaimie M. Van Norman 1, Weiping Zhang 1, Nellie A. Fauver 1, Mary Lou Spencer 1, and Leslie E. Sieburth 1*
1 Department of Biology, University of Utah, Salt Lake City, Utah 84112
* To whom correspondence should be addressed. E-mail: sieburth{at}biology.utah.edu.
To understand the mechanisms controlling vein patterning in Arabidopsis thaliana, we analyzed two phenotypically similar mutants, varicose (vcs) and trident (tdt). We had previously identified VCS, and recently, human VCS was shown to function in mRNA decapping. Here, we report that TDT encodes the mRNA-decapping enzyme. VCS and TDT function together in small cytoplasmic foci that appear to be processing bodies. To understand the developmental requirements for mRNA decapping, we characterized the vcs and tdt phenotypes. These mutants were small and chlorotic, with severe defects in shoot apical meristem formation and cotyledon vein patterning. Many capped mRNAs accumulated in tdt and vcs mutants, but surprisingly, some mRNAs were specifically depleted. In addition, loss of decapping arrested the decay of some mRNAs, while others showed either modest or no decay defects, suggesting that mRNAs may show specificity for particular decay pathways (3' to 5' and 5' to 3'). Furthermore, the severe block to postembryonic development in vcs and tdt and the accompanying accumulation of embryonic mRNAs indicate that decapping is important for the embryo-to-seedling developmental transition.
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