Plant Cell SoftGenetics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on February 16, 2007; 10.1105/tpc.106.048140

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
19/2/509    most recent
tpc.106.048140v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Binder, B. M.
Right arrow Articles by Vierstra, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Binder, B. M.
Right arrow Articles by Vierstra, R. D.
Agricola
Right arrow Articles by Binder, B. M.
Right arrow Articles by Vierstra, R. D.

Received October 11, 2006
Returned for revision December 8, 2006
Accepted January 25, 2007

The Arabidopsis EIN3 Binding F-Box Proteins EBF1 and EBF2 Have Distinct but Overlapping Roles in Ethylene Signaling

Brad M. Binder 1, Joseph M. Walker 2, Jennifer M. Gagne 2, Thomas J. Emborg 2, Georg Hemmann 2, Anthony B. Bleecker 1, and Richard D. Vierstra 2*

1 Department of Botany, University of Wisconsin, Madison, Wisconsin 53706
2 Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706

* To whom correspondence should be addressed. E-mail: vierstra{at}wisc.edu.

Ethylene signaling in Arabidopsis thaliana converges on the ETHYLENE-INSENSITIVE3 (EIN3)/EIN3-Like (EIL) transcription factors to induce various responses. EIN3 BINDING F-BOX1 (EBF1) and EBF2 were recently shown to function in ethylene perception by regulating EIN3/EIL turnover. In the absence of ethylene, EIN3 and possibly other EIL proteins are targeted for ubiquitination and subsequent degradation by Cullin 1-based E3 complexes containing EBF1 and 2. Ethylene appears to block this ubiquitination, allowing EIN3/EIL levels to rise and mediate ethylene signaling. Through analysis of mutant combinations affecting accumulation of EBF1, EBF2, EIN3, and EIL1, we show that EIN3 and EIL1 are the main targets of EBF1/2. Kinetic analyses of hypocotyl growth inhibition in response to ethylene and growth recovery after removal of the hormone revealed that EBF1 and 2 have temporally distinct but overlapping roles in modulating ethylene perception. Whereas EBF1 plays the main role in air and during the initial phase of signaling, EBF2 plays a more prominent role during the latter stages of the response and the resumption of growth following ethylene removal. Through their coordinated control of EIN3/EIL1 levels, EBF1 and EBF2 fine-tune ethylene responses by repressing signaling in the absence of the hormone, dampening signaling at high hormone concentrations, and promoting a more rapid recovery after ethylene levels dissipate.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X. Yang, U. C. Kalluri, S. Jawdy, L. E. Gunter, T. Yin, T. J. Tschaplinski, D. J. Weston, P. Ranjan, and G. A. Tuskan
The F-Box Gene Family Is Expanded in Herbaceous Annual Plants Relative to Woody Perennial Plants
Plant Physiology, November 1, 2008; 148(3): 1189 - 1200.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. H. Doelling, A. R. Phillips, G. Soyler-Ogretim, J. Wise, J. Chandler, J. Callis, M. S. Otegui, and R. D. Vierstra
The Ubiquitin-Specific Protease Subfamily UBP3/UBP4 Is Essential for Pollen Development and Transmission in Arabidopsis
Plant Physiology, November 1, 2007; 145(3): 801 - 813.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. J. Gingerich, K. Hanada, S.-H. Shiu, and R. D. Vierstra
Large-Scale, Lineage-Specific Expansion of a Bric-a-Brac/Tramtrack/Broad Complex Ubiquitin-Ligase Gene Family in Rice
PLANT CELL, August 1, 2007; 19(8): 2329 - 2348.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. M. Robles, J. S. Wampole, M. J. Christians, and P. B. Larsen
Arabidopsis enhanced ethylene response 4 encodes an EIN3-interacting TFIID transcription factor required for proper ethylene response, including ERF1 induction
J. Exp. Bot., July 1, 2007; 58(10): 2627 - 2639.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2007 by the American Society of Plant Biologists