Plant Cell Advance Online Publication
Published on April 27, 2007; 10.1105/tpc.106.048355
OPEN ACCESS ARTICLE
Received October 18, 2006
Returned for revision March 4, 2007
Accepted April 10, 2007
Plant Mitochondrial Recombination Surveillance Requires Unusual RecA and MutS Homologs
Vikas Shedge 1, Maria Arrieta-Montiel 1, Alan C. Christensen 2, and Sally A. Mackenzie 3*
1 Plant Science Initiative, University of Nebraska, Lincoln, Nebraska 68588-0660
2 School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0660
3 Plant Science Initiative, University of Nebraska, Lincoln, Nebraska 68588-0660; School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0660
* To whom correspondence should be addressed. E-mail: smackenzie2{at}unl.edu.
For >20 years, the enigmatic behavior of plant mitochondrial genomes has been well described but not well understood. Chimeric genes appear, and occasionally are differentially replicated or expressed, with significant effects on plant phenotype, most notably on male fertility, yet the mechanisms of DNA replication, chimera formation, and recombination have remained elusive. Using mutations in two important genes of mitochondrial DNA metabolism, we have observed reproducible asymmetric recombination events occurring at specific locations in the mitochondrial genome. Based on these experiments and existing models of double-strand break repair, we propose a model for plant mitochondrial DNA replication, chimeric gene formation, and the illegitimate recombination events that lead to stoichiometric changes. We also address the physiological and developmental effects of aberrant events in mitochondrial genome maintenance, showing that mitochondrial genome rearrangements, when controlled, influence plant reproduction, but when uncontrolled, lead to aberrant growth phenotypes and dramatic reduction of the cell cycle.
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