Plant Cell BIOBASE Corporation
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on April 6, 2007; 10.1105/tpc.106.049270

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
19/4/1313    most recent
tpc.106.049270v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miura, E.
Right arrow Articles by Sakamoto, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miura, E.
Right arrow Articles by Sakamoto, W.
Agricola
Right arrow Articles by Miura, E.
Right arrow Articles by Sakamoto, W.

Received November 29, 2006
Returned for revision March 4, 2007
Accepted March 16, 2007

The Balance between Protein Synthesis and Degradation in Chloroplasts Determines Leaf Variegation in Arabidopsis yellow variegated Mutants

Eiko Miura 1, Yusuke Kato 1, Ryo Matsushima 1, Verónica Albrecht 2, Soumaya Laalami 3, and Wataru Sakamoto 1*

1 Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan
2 Institute of Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland
3 Institut Jacques Monod, Centre National de la Recherche Scientifique, Universités Paris 6 et Paris 7, 75251 Paris, France

* To whom correspondence should be addressed. E-mail: saka{at}rib.okayama-u.ac.jp.

An Arabidopsis thaliana leaf-variegated mutant yellow variegated2 (var2) results from loss of FtsH2, a major component of the chloroplast FtsH complex. FtsH is an ATP-dependent metalloprotease in thylakoid membranes and degrades several chloroplastic proteins. To understand the role of proteolysis by FtsH and mechanisms leading to leaf variegation, we characterized the second-site recessive mutation fu-gaeri1 (fug1) that suppressed leaf variegation of var2. Map-based cloning and subsequent characterization of the FUG1 locus demonstrated that it encodes a protein homologous to prokaryotic translation initiation factor 2 (cpIF2) located in chloroplasts. We show evidence that cpIF2 indeed functions in chloroplast protein synthesis in vivo. Suppression of leaf variegation by fug1 is observed not only in var2 but also in var1 (lacking FtsH5) and var1 var2. Thus, suppression of leaf variegation caused by loss of FtsHs is most likely attributed to reduced protein synthesis in chloroplasts. This hypothesis was further supported by the observation that another viable mutation in chloroplast translation elongation factor G also suppresses leaf variegation in var2. We propose that the balance between protein synthesis and degradation is one of the determining factors leading to the variegated phenotype in Arabidopsis leaves.




This article has been cited by other articles:


Home page
 Plant CellHome page
F. Yu, X. Liu, M. Alsheikh, S. Park, and S. Rodermel
Mutations in SUPPRESSOR OF VARIEGATION1, a Factor Required for Normal Chloroplast Translation, Suppress var2-Mediated Leaf Variegation in Arabidopsis
PLANT CELL, July 1, 2008; 20(7): 1786 - 1804.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Yoshida, C. Watanabe, Y. Kato, W. Sakamoto, and K. Noguchi
Influence of Chloroplastic Photo-Oxidative Stress on Mitochondrial Alternative Oxidase Capacity and Respiratory Properties: A Case Study with Arabidopsis yellow variegated 2
Plant Cell Physiol., April 1, 2008; 49(4): 592 - 603.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2007 by the American Society of Plant Biologists