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Plant Cell Advance Online Publication
Published on November 21, 2007; 10.1105/tpc.107.050427

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Received January 15, 2007
Returned for revision October 7, 2007
Accepted October 31, 2007

Structural and Functional Analysis of SGT1 Reveals That Its Interaction with HSP90 Is Required for the Accumulation of Rx, an R Protein Involved in Plant Immunity

Marta Botër 1, Béatrice Amigues 2, Jack Peart 1, Christian Breuer 1, Yasuhiro Kadota 3, Catarina Casais 1, Geoffrey Moore 4, Colin Kleanthous 5, Francoise Ochsenbein 2, Ken Shirasu 6*, and Raphaël Guerois 2

1 Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
2 Commissariat à l'Energie Atomique, Institut de Biologie et Technologies de Saclay, and Centre National de la Recherche Scientifique, Gif-sur-Yvette F-91191, France
3 RIKEN Plant Science Center, Tsurumi-ku, Yokohama, 230-0045 Japan
4 School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom
5 Department of Biology, University of York, York YO10 5YW, United Kingdom
6 Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom; RIKEN Plant Science Center, Tsurumi-ku, Yokohama, 230-0045 Japan

* To whom correspondence should be addressed. E-mail: ken.shirasu{at}psc.riken.jp.

SGT1 (for suppressor of G2 allele of skp1) and RAR1 (for required for Mla12 resistance) are highly conserved eukaryotic proteins that interact with the molecular chaperone HSP90 (for heat shock protein90). In plants, SGT1, RAR1, and HSP90 are essential for disease resistance triggered by a number of resistance (R) proteins. Here, we present structural and functional characterization of plant SGT1 proteins. Random mutagenesis of Arabidopsis thaliana SGT1b revealed that its CS (for CHORD-SGT1) and SGS (for SGT1 specific) domains are essential for disease resistance. NMR-based interaction surface mapping and mutational analyses of the CS domain showed that the CHORD II domain of RAR1 and the N-terminal domain of HSP90 interact with opposite sides of the CS domain. Functional analysis of the CS mutations indicated that the interaction between SGT1 and HSP90 is required for the accumulation of Rx, a potato (Solanum tuberosum) R protein. Biochemical reconstitution experiments suggest that RAR1 may function to enhance the SGT1–HSP90 interaction by promoting ternary complex formation.






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