Plant Cell Advance Online Publication
Published on August 3, 2007; 10.1105/tpc.107.050708
OPEN ACCESS ARTICLE
Received January 26, 2007
Returned for revision July 2, 2007
Accepted July 10, 2007
A Downstream Mediator in the Growth Repression Limb of the Jasmonate Pathway
Yuanxin Yan 1, Stéphanie Stolz 1, Aurore Chételat 1, Philippe Reymond 1, Marco Pagni 2, Lucie Dubugnon 1, and Edward E. Farmer 1*
1 Department of Plant Molecular Biology, University of Lausanne, Biophore, CH-1015 Lausanne, Switzerland
2 Swiss Institute of Bioinformatics, Vital-IT Group, Génopode, CH-1015 Lausanne, Switzerland
* To whom correspondence should be addressed. E-mail: edward.farmer{at}unil.ch.
Wounding plant tissues initiates large-scale changes in transcription coupled to growth arrest, allowing resource diversion for defense. These processes are mediated in large part by the potent lipid regulator jasmonic acid (JA). Genes selected from a list of wound-inducible transcripts regulated by the jasmonate pathway were overexpressed in Arabidopsis thaliana, and the transgenic plants were then assayed for sensitivity to methyl jasmonate (MeJA). When grown in the presence of MeJA, the roots of plants overexpressing a gene of unknown function were longer than those of wild-type plants. When transcript levels for this gene, which we named JASMONATE-ASSOCIATED1 (JAS1), were reduced by RNA interference, the plants showed increased sensitivity to MeJA and growth was inhibited. These gain- and loss-of-function assays suggest that this gene acts as a repressor of JA-inhibited growth. An alternative transcript from the gene encoding a second protein isoform with a longer C terminus failed to repress jasmonate sensitivity. This identified a conserved C-terminal sequence in JAS1 and related genes, all of which also contain Zim motifs and many of which are jasmonate-regulated. Both forms of JAS1 were found to localize to the nucleus in transient expression assays. Physiological tests of growth responses after wounding were consistent with the fact that JAS1 is a repressor of JA-regulated growth retardation.
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