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Plant Cell Advance Online Publication
Published on June 22, 2007; 10.1105/tpc.107.050849

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Received February 6, 2007
Returned for revision April 30, 2007
Accepted May 29, 2007

Inactivation of Thioredoxin Reductases Reveals a Complex Interplay between Thioredoxin and Glutathione Pathways in Arabidopsis Development

Jean-Philippe Reichheld 1*, Mehdi Khafif 1, Christophe Riondet 1, Michel Droux 2, Géraldine Bonnard 3, and Yves Meyer 1

1 Laboratoire Génome et Développement des Plantes, Université de Perpignan, Unité Mixte de Recherche, Centre National de la Recherche Scientifique 5096, 66860 Perpignan, France
2 Laboratoire Mixte Centre National de la Recherche Scientifique/Bayer CropScience Unité Mixte de Recherche 5240, 69263 Lyon Cedex 9, France
3 Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche, Centre National de la Recherche Scientifique 2357, Université Louis Pasteur, 67084 Strasbourg, France

* To whom correspondence should be addressed. E-mail: jpr{at}univ-perp.fr.

NADPH-dependent thioredoxin reductases (NTRs) are key regulatory enzymes determining the redox state of the thioredoxin system. The Arabidopsis thaliana genome has two genes coding for NTRs (NTRA and NTRB), both of which encode mitochondrial and cytosolic isoforms. Surprisingly, plants of the ntra ntrb knockout mutant are viable and fertile, although with a wrinkled seed phenotype, slower plant growth, and pollen with reduced fitness. Thus, in contrast with mammals, our data demonstrate that neither cytosolic nor mitochondrial NTRs are essential in plants. Nevertheless, in the double mutant, the cytosolic thioredoxin h3 is only partially oxidized, suggesting an alternative mechanism for thioredoxin reduction. Plant growth in ntra ntrb plants is hypersensitive to buthionine sulfoximine (BSO), a specific inhibitor of glutathione biosynthesis, and thioredoxin h3 is totally oxidized under this treatment. Interestingly, this BSO-mediated growth arrest is fully reversible, suggesting that BSO induces a growth arrest signal but not a toxic accumulation of activated oxygen species. Moreover, crossing ntra ntrb with rootmeristemless1, a mutant blocked in root growth due to strongly reduced glutathione synthesis, led to complete inhibition of both shoot and root growth, indicating that either the NTR or the glutathione pathway is required for postembryonic activity in the apical meristem.






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