Plant Cell Advance Online Publication
Published on April 6, 2007; 10.1105/tpc.107.051441
Received March 2, 2007
Returned for revision March 2, 2007
Accepted March 22, 2007
The DELLA Domain of GA INSENSITIVE Mediates the Interaction with the GA INSENSITIVE DWARF1A Gibberellin Receptor of Arabidopsis
Björn C. Willige 1, Soumya Ghosh 1, Carola Nill 1, Melina Zourelidou 1, Esther M.N. Dohmann 1, Andreas Maier 1, and Claus Schwechheimer 1*
1 Department of Developmental Genetics, Center for Plant Molecular Biology, Tübingen University, 72076 Tübingen, Germany
* To whom correspondence should be addressed. E-mail: claus.schwechheimer{at}zmbp.uni-tuebingen.de.
Gibberellic acid (GA) promotes seed germination, elongation growth, and flowering time in plants. GA responses are repressed by DELLA proteins, which contain an N-terminal DELLA domain essential for GA-dependent proteasomal degradation of DELLA repressors. Mutations of or within the DELLA domain of DELLA repressors have been described for species including Arabidopsis thaliana, wheat (Triticum aestivum), maize (Zea mays), and barley (Hordeum vulgare), and we show that these mutations confer GA insensitivity when introduced into the Arabidopsis GA INSENSITIVE (GAI) DELLA repressor. We also demonstrate that Arabidopsis mutants lacking the three GA INSENSITIVE DWARF1 (GID1) GA receptor genes are GA insensitive with respect to GA-promoted growth responses, GA-promoted DELLA repressor degradation, and GA-regulated gene expression. Our genetic interaction studies indicate that GAI and its close homolog REPRESSOR OF ga1-3 are the major growth repressors in a GA receptor mutant background. We further demonstrate that the GA insensitivity of the GAI DELLA domain mutants is explained in all cases by the inability of the mutant proteins to interact with the GID1A GA receptor. Since we found that the GAI DELLA domain alone can mediate GA-dependent GID1A interactions, we propose that the DELLA domain functions as a receiver domain for activated GA receptors.
This article has been cited by other articles:
|
|
|
|
 
J. Argyris, P. Dahal, E. Hayashi, D. W. Still, and K. J. Bradford
Genetic Variation for Lettuce Seed Thermoinhibition Is Associated with Temperature-Sensitive Expression of Abscisic Acid, Gibberellin, and Ethylene Biosynthesis, Metabolism, and Response Genes
Plant Physiology,
October 1, 2008;
148(2):
926 - 947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ariizumi, K. Murase, T.-p. Sun, and C. M. Steber
Proteolysis-Independent Downregulation of DELLA Repression in Arabidopsis by the Gibberellin Receptor GIBBERELLIN INSENSITIVE DWARF1
PLANT CELL,
September 1, 2008;
20(9):
2447 - 2459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ueguchi-Tanaka, K. Hirano, Y. Hasegawa, H. Kitano, and M. Matsuoka
Release of the Repressive Activity of Rice DELLA Protein SLR1 by Gibberellin Does Not Require SLR1 Degradation in the gid2 Mutant
PLANT CELL,
September 1, 2008;
20(9):
2437 - 2446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Spartz and W. M. Gray
Plant hormone receptors: new perceptions
Genes & Dev.,
August 15, 2008;
22(16):
2139 - 2148.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Achard, F. Gong, S. Cheminant, M. Alioua, P. Hedden, and P. Genschik
The Cold-Inducible CBF1 Factor-Dependent Signaling Pathway Modulates the Accumulation of the Growth-Repressing DELLA Proteins via Its Effect on Gibberellin Metabolism
PLANT CELL,
August 1, 2008;
20(8):
2117 - 2129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2007
J. Exp. Bot.,
July 18, 2008;
(2008)
ern109v1.
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Hou, W.-W. Hu, L. Shen, L. Y. C. Lee, Z. Tao, J.-H. Han, and H. Yu
Global Identification of DELLA Target Genes during Arabidopsis Flower Development
Plant Physiology,
July 1, 2008;
147(3):
1126 - 1142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Desgagne-Penix and V. M. Sponsel
Expression of gibberellin 20-oxidase1 (AtGA20ox1) in Arabidopsis seedlings with altered auxin status is regulated at multiple levels
J. Exp. Bot.,
May 1, 2008;
59(8):
2057 - 2070.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M. Chandler, C. A. Harding, A. R. Ashton, M. D. Mulcair, N. E. Dixon, and L. N. Mander
Characterization of Gibberellin Receptor Mutants of Barley (Hordeum vulgare L.)
Mol Plant,
March 1, 2008;
1(2):
285 - 294.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kobayashi and D. Weigel
Move on up, it's time for change mobile signals controlling photoperiod-dependent flowering
Genes & Dev.,
October 1, 2007;
21(19):
2371 - 2384.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zentella, Z.-L. Zhang, M. Park, S. G. Thomas, A. Endo, K. Murase, C. M. Fleet, Y. Jikumaru, E. Nambara, Y. Kamiya, et al.
Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Arabidopsis
PLANT CELL,
October 1, 2007;
19(10):
3037 - 3057.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hirano, M. Nakajima, K. Asano, T. Nishiyama, H. Sakakibara, M. Kojima, E. Katoh, H. Xiang, T. Tanahashi, M. Hasebe, et al.
The GID1-Mediated Gibberellin Perception Mechanism Is Conserved in the Lycophyte Selaginella moellendorffii but Not in the Bryophyte Physcomitrella patens
PLANT CELL,
October 1, 2007;
19(10):
3058 - 3079.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Eckardt
GA Perception and Signal Transduction: Molecular Interactions of the GA Receptor GID1 with GA and the DELLA Protein SLR1 in Rice
PLANT CELL,
July 1, 2007;
19(7):
2095 - 2097.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ueguchi-Tanaka, M. Nakajima, E. Katoh, H. Ohmiya, K. Asano, S. Saji, X. Hongyu, M. Ashikari, H. Kitano, I. Yamaguchi, et al.
Molecular Interactions of a Soluble Gibberellin Receptor, GID1, with a Rice DELLA Protein, SLR1, and Gibberellin
PLANT CELL,
July 1, 2007;
19(7):
2140 - 2155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
