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Plant Cell Advance Online Publication
Published on July 27, 2007; 10.1105/tpc.107.051516

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Received March 6, 2007
Returned for revision June 19, 2007
Accepted July 5, 2007

Light-Independent Phytochrome Signaling Mediated by Dominant GAF Domain Tyrosine Mutants of Arabidopsis Phytochromes in Transgenic Plants

Yi-shin Su 1 and J. Clark Lagarias 1*

1 Section of Molecular and Cellular Biology, University of California, Davis, California 95616

* To whom correspondence should be addressed. E-mail: jclagarias{at}ucdavis.edu.

The photoreversibility of plant phytochromes enables continuous surveillance of the ambient light environment. Through expression of profluorescent, photoinsensitive Tyr-to-His mutant alleles of Arabidopsis thaliana phytochrome B (PHYBY276H) and Arabidopsis phytochrome A (PHYAY242H) in transgenic Arabidopsis plants, we demonstrate that photoconversion is not a prerequisite for phytochrome signaling. PHYBY276H-expressing plants exhibit chromophore-dependent constitutive photomorphogenesis, light-independent phyBY276H nuclear localization, constitutive activation of genes normally repressed in darkness, and light-insensitive seed germination. Fluence rate analyses of transgenic plants expressing PHYBY276H, PHYAY242H, and other YGAF mutant alleles of PHYB demonstrate that a range of altered light-signaling activities are associated with mutation of this residue. We conclude that the universally conserved GAF domain Tyr residue, with which the bilin chromophore is intimately associated, performs a critical role in coupling light perception to signal transduction by plant phytochromes.




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H. Shen, L. Zhu, A. Castillon, M. Majee, B. Downie, and E. Huq
Light-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 from Arabidopsis Depend upon Its Direct Physical Interactions with Photoactivated Phytochromes
PLANT CELL, June 1, 2008; 20(6): 1586 - 1602.
[Abstract] [Full Text] [PDF]


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