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Plant Cell Advance Online Publication
Published on January 4, 2008; 10.1105/tpc.107.051862

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Received March 24, 2007
Returned for revision December 6, 2007
Accepted December 17, 2007

Arabidopsis UEV1D Promotes Lysine-63–Linked Polyubiquitination and Is Involved in DNA Damage Response

Rui Wen 1, J. Antonio Torres-Acosta 2, Landon Pastushok 1, Xiaoqin Lai 1, Lindsay Pelzer 1, Hong Wang 2*, and Wei Xiao 1

1 Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E5
2 Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E5

* To whom correspondence should be addressed. E-mail: hong.wang{at}usask.ca.

DNA damage tolerance (DDT) in budding yeast requires Lys-63–linked polyubiquitination of the proliferating cell nuclear antigen. The ubiquitin-conjugating enzyme Ubc13 and the Ubc enzyme variant (Uev) methyl methanesulfonate2 (Mms2) are required for this process. Mms2 homologs have been found in all eukaryotic genomes examined; however, their roles in multicellular eukaryotes have not been elucidated. We report the isolation and characterization of four UEV1 genes from Arabidopsis thaliana. All four Uev1 proteins can form a stable complex with At Ubc13 or with Ubc13 from yeast or human and can promote Ubc13-mediated Lys-63 polyubiquitination. All four Uev1 proteins can replace yeast MMS2 DDT functions in vivo. Although these genes are ubiquitously expressed in most tissues, UEV1D appears to express at a much higher level in germinating seeds and in pollen. We obtained and characterized two uev1d null mutant T-DNA insertion lines. Compared with wild-type plants, seeds from uev1d null plants germinated poorly when treated with a DNA-damaging agent. Those that germinated grew slower, and the majority ceased growth within 2 weeks. Pollen from uev1d plants also displayed a moderate but significant decrease in germination in the presence of DNA damage. This report links Ubc13-Uev with functions in DNA damage response in Arabidopsis.




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[Abstract] [Full Text] [PDF]


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