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Plant Cell Advance Online Publication
Published on March 18, 2008; 10.1105/tpc.107.052043

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Received April 12, 2007
Returned for revision February 14, 2008
Accepted March 3, 2008

Cellulose Synthesis in Phytophthora infestans Is Required for Normal Appressorium Formation and Successful Infection of Potato

Laura J. Grenville-Briggs 1, Victoria L. Anderson 1, Johanna Fugelstad 2, Anna O. Avrova 3, Jamel Bouzenzana 4, Alison Williams 1, Stephan Wawra 1, Stephen C. Whisson 3, Paul R.J. Birch 3, Vincent Bulone 2, and Pieter van West 1*

1 Aberdeen Oomycete Group, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD, United Kingdom
2 School of Biotechnology, Royal Institute of Technology, AlbaNova University Center, Stockholm SE-106 91, Sweden
3 Plant-Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom
4 Organisation et Dynamique des Membranes Biologiques, Unité Mixte de Recherche 5246, Centre National de la Recherche Scientifique, Bâtiment Chevreul, Université Lyon I, 69622 Villeurbanne cedex, France

* To whom correspondence should be addressed. E-mail: p.vanwest{at}abdn.ac.uk.

Cellulose, the important structural compound of cell walls, provides strength and rigidity to cells of numerous organisms. Here, we functionally characterize four cellulose synthase genes (CesA) in the oomycete plant pathogen Phytophthora infestans, the causal agent of potato (Solanum tuberosum) late blight. Three members of this new protein family contain Pleckstrin homology domains and form a distinct phylogenetic group most closely related to the cellulose synthases of cyanobacteria. Expression of all four genes is coordinately upregulated during pre- and early infection stages of potato. Inhibition of cellulose synthesis by 2,6-dichlorobenzonitrile leads to a dramatic reduction in the number of normal germ tubes with appressoria, severe disruption of the cell wall in the preinfection structures, and a complete loss of pathogenicity. Silencing of the entire gene family in P. infestans with RNA interference leads to a similar disruption of the cell wall surrounding appressoria and an inability to form typical functional appressoria. In addition, the cellulose content of the cell walls of the silenced lines is >50% lower than in the walls of the nonsilenced lines. Our data demonstrate that the isolated genes are involved in cellulose biosynthesis and that cellulose synthesis is essential for infection by P. infestans.




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