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Plant Cell Advance Online Publication
Published on July 6, 2007; 10.1105/tpc.107.052332

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Received May 24, 2007
Returned for revision May 24, 2007
Accepted June 14, 2007

An Ustilago maydis Gene Involved in H2O2 Detoxification Is Required for Virulence

Lázaro Molina 1 and Regine Kahmann 1*

1 Max Planck Institute for Terrestrial Microbiology, D-35043 Marburg, Germany

* To whom correspondence should be addressed. E-mail: kahmann{at}mpi-marburg.mpg.de.

The fungus Ustilago maydis is a biotrophic pathogen of maize (Zea mays). In its genome we have identified an ortholog of YAP1 (for Yeast AP-1-like) from Saccharomyces cerevisae that regulates the oxidative stress response in this organism. yap1 mutants of U. maydis displayed higher sensitivity to H2O2 than wild-type cells, and their virulence was significantly reduced. U. maydis yap1 could partially complement the H2O2 sensitivity of a yap1 deletion mutant of S. cerevisiae, and a Yap1-green fluorescent protein fusion protein showed nuclear localization after H2O2 treatment, suggesting that Yap1 in U. maydis functions as a redox sensor. Mutations in two Cys residues prevented accumulation in the nucleus, and the respective mutant strains showed the same virulence phenotype as {Delta}yap1 mutants. Diamino benzidine staining revealed an accumulation of H2O2 around yap1 mutant hyphae, which was absent in the wild type. Inhibition of the plant NADPH oxidase prevented this accumulation and restored virulence. During the infection, Yap1 showed nuclear localization after penetration up to 2 to 3 d after infection. Through array analysis, a large set of Yap1-regulated genes were identified and these included two peroxidase genes. Deletion mutants of these genes were attenuated in virulence. These results suggest that U. maydis is using its Yap1-controlled H2O2 detoxification system for coping with early plant defense responses.




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