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Plant Cell Advance Online Publication
Published on September 21, 2007; 10.1105/tpc.107.053678

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Received June 14, 2007
Returned for revision August 26, 2007
Accepted August 28, 2007

The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis

Ruiqin Zhong 1, Elizabeth A. Richardson 1, and Zheng-Hua Ye 1*

1 Department of Plant Biology, University of Georgia, Athens, Georgia 30602

* To whom correspondence should be addressed. E-mail: zhye{at}plantbio.uga.edu.

We demonstrate that the Arabidopsis thaliana MYB46 transcription factor is a direct target of SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN1 (SND1), which is a key transcriptional activator regulating the developmental program of secondary wall biosynthesis. The MYB46 gene is expressed predominantly in fibers and vessels in stems, and its encoded protein is targeted to the nucleus and can activate transcription. MYB46 gene expression was shown to be regulated by SND1, and transactivation analysis demonstrated that SND1 as well as its close homologs were able to activate the MYB46 promoter. Electrophoretic mobility shift assays and chromatin immunoprecipitation experiments revealed that SND1 binds to the MYB46 promoter. Dominant repression of MYB46 caused a drastic reduction in the secondary wall thickening of fibers and vessels. Overexpression of MYB46 resulted in an activation of the biosynthetic pathways of cellulose, xylan, and lignin and concomitantly led to ectopic deposition of secondary walls in cells that are normally nonsclerenchymatous. In addition, the expression of two secondary wall–associated transcription factors, MYB85 and KNAT7, was highly upregulated by MYB46 overexpression. These results demonstrate that MYB46 is a direct target of SND1 and is another key player in the transcriptional network involved in the regulation of secondary wall biosynthesis in Arabidopsis.




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 Plant Cell PhysiolHome page
C. Lee, R. Zhong, E. A. Richardson, D. S. Himmelsbach, B. T. McPhail, and Z.-H. Ye
The PARVUS Gene is Expressed in Cells Undergoing Secondary Wall Thickening and is Essential for Glucuronoxylan Biosynthesis
Plant Cell Physiol., December 1, 2007; 48(12): 1659 - 1672.
[Abstract] [Full Text] [PDF]


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