Plant Cell Illumina, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on July 11, 2008; 10.1105/tpc.107.054007

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
20/7/1833    most recent
tpc.107.054007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Satoh, H.
Right arrow Articles by Nakamura, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Satoh, H.
Right arrow Articles by Nakamura, Y.
Agricola
Right arrow Articles by Satoh, H.
Right arrow Articles by Nakamura, Y.

Received July 19, 2007
Returned for revision June 2, 2008
Accepted June 23, 2008

Mutation of the Plastidial {alpha}-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm

Hikaru Satoh 1*, Kensuke Shibahara 1, Takashi Tokunaga 1, Aiko Nishi 1, Mikako Tasaki 1, Seon-Kap Hwang 2, Thomas W. Okita 2, Nanae Kaneko 3, Naoko Fujita 3, Mayumi Yoshida 3, Yuko Hosaka 3, Aya Sato 3, Yoshinori Utsumi 3, Takashi Ohdan 3, and Yasunori Nakamura 3

1 Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
2 Institute of Biological Chemistry, Washington State University, Pullman, Washington, 99164-6340
3 Faculty of Bioresource Science, Akita Prefectural University, Akita 010-0195, Japan

* To whom correspondence should be addressed. E-mail: hsatoh{at}agr.kyushu-u.ac.jp.

Plastidial phosphorylase (Pho1) accounts for ~96% of the total phosphorylase activity in developing rice (Oryza sativa) seeds. From mutant stocks induced by N-methyl-N-nitrosourea treatment, we identified plants with mutations in the Pho1 gene that are deficient in Pho1. Strikingly, the size of mature seeds and the starch content in these mutants showed considerable variation, ranging from shrunken to pseudonormal. The loss of Pho1 caused smaller starch granules to accumulate and modified the amylopectin structure. Variation in the morphological and biochemical phenotype of individual seeds was common to all 15 pho1-independent homozygous mutant lines studied, indicating that this phenotype was caused solely by the genetic defect. The phenotype of the pho1 mutation was temperature dependent. While the mutant plants grown at 30°C produced mainly plump seeds at maturity, most of the seeds from plants grown at 20°C were shrunken, with a significant proportion showing severe reduction in starch accumulation. These results strongly suggest that Pho1 plays a crucial role in starch biosynthesis in rice endosperm at low temperatures and that one or more other factors can complement the function of Pho1 at high temperatures.




This article has been cited by other articles:


Home page
 J Exp BotHome page
F. Liu, A. Makhmoudova, E. A. Lee, R. Wait, M. J. Emes, and I. J. Tetlow
The amylose extender mutant of maize conditions novel protein-protein interactions between starch biosynthetic enzymes in amyloplasts
J. Exp. Bot., November 1, 2009; 60(15): 4423 - 4440.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Szydlowski, P. Ragel, S. Raynaud, M. M. Lucas, I. Roldan, M. Montero, F. J. Munoz, M. Ovecka, A. Bahaji, V. Planchot, et al.
Starch Granule Initiation in Arabidopsis Requires the Presence of Either Class IV or Class III Starch Synthases
PLANT CELL, August 1, 2009; 21(8): 2443 - 2457.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. V. Radchuk, L. Borisjuk, N. Sreenivasulu, K. Merx, H.-P. Mock, H. Rolletschek, U. Wobus, and W. Weschke
Spatiotemporal Profiling of Starch Biosynthesis and Degradation in the Developing Barley Grain
Plant Physiology, May 1, 2009; 150(1): 190 - 204.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2008 by the American Society of Plant Biologists