Plant Cell Advance Online Publication
Published on November 21, 2007; 10.1105/tpc.107.054536
Received August 1, 2007
Returned for revision September 30, 2007
Accepted October 29, 2007
Arabidopsis MALE STERILITY1 Encodes a PHD-Type Transcription Factor and Regulates Pollen and Tapetum Development
Takuya Ito 1, Noriko Nagata 2, Yoshu Yoshiba 3, Masaru Ohme-Takagi 4, Hong Ma 5*, and Kazuo Shinozaki 6
1 Antibiotics Laboratory, RIKEN, Tsukuba 305-0074, Japan; Department of Biology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802
2 Department of Chemical Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo, Tokyo 112-8681, Japan
3 Central Research Laboratory, Advanced Research Laboratory, Hitachi, Hatoyama, Saitama 350-0395, Japan
4 Gene Regulation Research Group, Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan
5 Department of Biology, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802
6 Gene Discovery Research Group, RIKEN Plant Science Center, Yokohama 230-0045, Japan
* To whom correspondence should be addressed. E-mail: hxm16{at}psu.edu.
The Arabidopsis thaliana MALE STERILITY1 (MS1) gene encodes a nuclear protein with Leu zipper–like and PHD-finger motifs and is important for postmeiotic pollen development. Here, we examined MS1 function using both cell biological and molecular biological approaches. We introduced a fusion construct of MS1 and a transcriptional repression domain (MS1-SRDX) into wild-type Arabidopsis, and the transgenic plants showed a semisterile phenotype similar to that of ms1. Since the repression domain can convert various kinds of transcriptional activators to dominant repressors, this suggested that MS1 functioned as a transcriptional activator. The Leu zipper–like region and the PHD motif were required for the MS1 function. Phenotypic analysis of the ms1 mutant and the MS1-SRDX transgenic Arabidopsis indicated that MS1 was involved in formation of pollen exine and pollen cytosolic components as well as tapetum development. Next, we searched for MS1 downstream genes by analyzing publicly available microarray data and identified 95 genes affected by MS1. Using a transgenic ms1 plant showing dexamethasone-inducible recovery of fertility, we further examined whether these genes were immediately downstream of MS1. From these results, we discuss a role of MS1 in pollen and tapetum development and the conservation of MS1 function in flowering plants.
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