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Plant Cell Advance Online Publication
Published on December 14, 2007; 10.1105/tpc.107.054759

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Received August 3, 2007
Returned for revision November 10, 2007
Accepted November 18, 2007

Gibberellin Regulates Pollen Viability and Pollen Tube Growth in Rice

Tory Chhun 1, Koichiro Aya 2, Kenji Asano 2, Eiji Yamamoto 1, Yoichi Morinaka 3, Masao Watanabe 4, Hidemi Kitano 1, Motoyuki Ashikari 1, Makoto Matsuoka 1*, and Miyako Ueguchi-Tanaka 1

1 Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan
2 Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan; Japan Society for the Promotion of Science, Chiyoda, Tokyo 102-8472, Japan
3 Honda Research Institute Japan, Kazusa-Kamatari, Kisarazu-shi, Chiba 292-0818, Japan
4 Graduate School of Life Science, Tohoku University, Aoba, Sendai 980-8577, Japan

* To whom correspondence should be addressed. E-mail: makoto{at}nuagr1.agr.nagoya-u.ac.jp.

Gibberellins (GAs) play many biological roles in higher plants. We collected and performed genetic analysis on rice (Oryza sativa) GA-related mutants, including GA-deficient and GA-insensitive mutants. Genetic analysis of the mutants revealed that rice GA-deficient mutations are not transmitted as Mendelian traits to the next generation following self-pollination of F1 heterozygous plants, although GA-insensitive mutations are transmitted normally. To understand these differences in transmission, we examined the effect of GA on microsporogenesis and pollen tube elongation in rice using new GA-deficient and GA-insensitive mutants that produce semifertile flowers. Phenotypic analysis revealed that the GA-deficient mutant reduced pollen elongation1 is defective in pollen tube elongation, resulting in a low fertilization frequency, whereas the GA-insensitive semidominant mutant Slr1-d3 is mainly defective in viable pollen production. Quantitative RT-PCR revealed that GA biosynthesis genes tested whose mutations are transmitted to the next generation at a lower frequency are preferentially expressed after meiosis during pollen development, but expression is absent or very low before the meiosis stage, whereas GA signal-related genes are actively expressed before meiosis. Based on these observations, we predict that the transmission of GA-signaling genes occurs in a sporophytic manner, since the protein products and/or mRNA transcripts of these genes may be introduced into pollen-carrying mutant alleles, whereas GA synthesis genes are transmitted in a gametophytic manner, since these genes are preferentially expressed after meiosis.




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