Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on March 14, 2008; 10.1105/tpc.107.054767

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
20/3/658    most recent
tpc.107.054767v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poulsen, L. R.
Right arrow Articles by Palmgren, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poulsen, L. R.
Right arrow Articles by Palmgren, M. G.
Agricola
Right arrow Articles by Poulsen, L. R.
Right arrow Articles by Palmgren, M. G.

Received August 3, 2007
Returned for revision January 21, 2008
Accepted February 25, 2008

The Arabidopsis P4-ATPase ALA3 Localizes to the Golgi and Requires a {beta}-Subunit to Function in Lipid Translocation and Secretory Vesicle Formation

Lisbeth Rosager Poulsen 1, Rosa Laura López-Marqués 1*, Stephen C. McDowell 2, Juha Okkeri 3, Dirk Licht 3, Alexander Schulz 4, Thomas Pomorski 3, Jeffrey F. Harper 2, and Michael Gjedde Palmgren 1

1 Centre for Membrane Pumps in Cells and Disease–PUMPKIN, Danish National Research Foundation, University of Copenhagen, DK-1871 Frederiksberg C, Denmark; Department of Plant Biology, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
2 Biochemistry Department MS200, University of Nevada, Reno, Nevada 89557
3 Humboldt-University Berlin, Faculty of Mathematics and Natural Science I, Institute of Biology, 10115 Berlin, Germany
4 Department of Plant Biology, University of Copenhagen, DK-1871 Frederiksberg C, Denmark

* To whom correspondence should be addressed. E-mail: rlo{at}life.ku.dk.

Vesicle budding in eukaryotes depends on the activity of lipid translocases (P4-ATPases) that have been implicated in generating lipid asymmetry between the two leaflets of the membrane and in inducing membrane curvature. We show that Aminophospholipid ATPase3 (ALA3), a member of the P4-ATPase subfamily in Arabidopsis thaliana, localizes to the Golgi apparatus and that mutations of ALA3 result in impaired growth of roots and shoots. The growth defect is accompanied by failure of the root cap to release border cells involved in the secretion of molecules required for efficient root interaction with the environment, and ala3 mutants are devoid of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion. In yeast complementation experiments, ALA3 function requires interaction with members of a novel family of plant membrane-bound proteins, ALIS1 to ALIS5 (for ALA-Interacting Subunit), and in this host ALA3 and ALIS1 show strong affinity for each other. In planta, ALIS1, like ALA3, localizes to Golgi-like structures and is expressed in root peripheral columella cells. We propose that the ALIS1 protein is a {beta}-subunit of ALA3 and that this protein complex forms an important part of the Golgi machinery required for secretory processes during plant development.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
R. L. Lopez-Marques, L. R. Poulsen, S. Hanisch, K. Meffert, M. J. Buch-Pedersen, M. K. Jakobsen, T. G. Pomorski, and M. G. Palmgren
Intracellular Targeting Signals and Lipid Specificity Determinants of the ALA/ALIS P4-ATPase Complex Reside in the Catalytic ALA {alpha}-Subunit
Mol. Biol. Cell, March 1, 2010; 21(5): 791 - 801.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Xu, J. Okkeri, S. Hanisch, R.-Y. Hu, Q. Xu, T. G. Pomorski, and X.-Y. Ding
Identification of a novel mouse P4-ATPase family member highly expressed during spermatogenesis
J. Cell Sci., August 15, 2009; 122(16): 2866 - 2876.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Lenoir, P. Williamson, C. F. Puts, and J. C. M. Holthuis
Cdc50p Plays a Vital Role in the ATPase Reaction Cycle of the Putative Aminophospholipid Transporter Drs2p
J. Biol. Chem., July 3, 2009; 284(27): 17956 - 17967.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2008 by the American Society of Plant Biologists