Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on October 12, 2007; 10.1105/tpc.107.054999

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
19/10/3037    most recent
tpc.107.054999v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zentella, R.
Right arrow Articles by Sun, T.-p.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zentella, R.
Right arrow Articles by Sun, T.-p.
Agricola
Right arrow Articles by Zentella, R.
Right arrow Articles by Sun, T.-p.

Received August 11, 2007
Returned for revision September 14, 2007
Accepted September 20, 2007

Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Arabidopsis

Rodolfo Zentella 1, Zhong-Lin Zhang 1, Mehea Park 1, Stephen G. Thomas 1, Akira Endo 2, Kohji Murase 1, Christine M. Fleet 1, Yusuke Jikumaru 2, Eiji Nambara 2, Yuji Kamiya 2, and Tai-ping Sun 1*

1 Department of Biology, Duke University, Durham, North Carolina 27708
2 RIKEN Plant Science Center, Tsurumi-ku, Yokohama, 230-0045, Japan

* To whom correspondence should be addressed. E-mail: tps{at}duke.edu.

Bioactive gibberellins (GAs) are phytohormones that regulate growth and development throughout the life cycle of plants. DELLA proteins are conserved growth repressors that modulate all aspects of GA responses. These GA-signaling repressors are nuclear localized and likely function as transcriptional regulators. Recent studies demonstrated that GA, upon binding to its receptor, derepresses its signaling pathway by binding directly to DELLA proteins and targeting them for rapid degradation via the ubiquitin-proteasome pathway. Therefore, elucidating the signaling events immediately downstream of DELLA is key to our understanding of how GA controls plant development. Two sets of microarray studies followed by quantitative RT-PCR analysis allowed us to identify 14 early GA-responsive genes that are also early DELLA-responsive in Arabidopsis thaliana seedlings. Chromatin immunoprecipitation provided evidence for in vivo association of DELLA with promoters of eight of these putative DELLA target genes. Expression of all 14 genes was downregulated by GA and upregulated by DELLA. Our study reveals that DELLA proteins play two important roles in GA signaling: (1) they help establish GA homeostasis by direct feedback regulation on the expression of GA biosynthetic and GA receptor genes, and (2) they promote the expression of downstream negative components that are putative transcription factors/regulators or ubiquitin E2/E3 enzymes. In addition, one of the putative DELLA targets, XERICO, promotes accumulation of abscisic acid (ABA) that antagonizes GA effects. Therefore, DELLA may restrict GA-promoted processes by modulating both GA and ABA pathways.




This article has been cited by other articles:


Home page
 Plant CellHome page
D. H. Kim, S. Yamaguchi, S. Lim, E. Oh, J. Park, A. Hanada, Y. Kamiya, and G. Choi
SOMNUS, a CCCH-Type Zinc Finger Protein in Arabidopsis, Negatively Regulates Light-Dependent Seed Germination Downstream of PIL5
PLANT CELL, May 1, 2008; 20(5): 1260 - 1277.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. E. Weston, R. C. Elliott, D. R. Lester, C. Rameau, J. B. Reid, I. C. Murfet, and J. J. Ross
The Pea DELLA Proteins LA and CRY Are Important Regulators of Gibberellin Synthesis and Root Growth
Plant Physiology, May 1, 2008; 147(1): 199 - 205.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Toh, A. Imamura, A. Watanabe, K. Nakabayashi, M. Okamoto, Y. Jikumaru, A. Hanada, Y. Aso, K. Ishiyama, N. Tamura, et al.
High Temperature-Induced Abscisic Acid Biosynthesis and Its Role in the Inhibition of Gibberellin Action in Arabidopsis Seeds
Plant Physiology, March 1, 2008; 146(3): 1368 - 1385.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2007 by the American Society of Plant Biologists