Plant Cell Advance Online Publication Published on June 13, 2008; 10.1105/tpc.107.057380
Received December 4, 2007
Returned for revision April 30, 2008
Accepted May 28, 2008
Arabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene Expression
Feng Qin 1, Yoh Sakuma 2, Lam-Son Phan Tran 1, Kyonoshin Maruyama 1, Satoshi Kidokoro 2, Yasunari Fujita 1, Miki Fujita 3, Taishi Umezawa 3, Yoriko Sawano 4, Ken-ichi Miyazono 4, Masaru Tanokura 4, Kazuo Shinozaki 3, and Kazuko Yamaguchi-Shinozaki 2*
1 Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan
2 Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan; Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
3 RIKEN Plant Science Center, Tsurumi-ku, Yokohama 230-0045, Japan
4 Laboratory of Food Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan
* To whom correspondence should be addressed. E-mail: kazukoys{at}jircas.affrc.go.jp.
The DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) transcription factor controls water deficit–inducible gene expression and requires posttranslational modification for its activation. The activation mechanism is not well understood; however, the stability of this protein in the nucleus was recently found to be important for its activation. Here, we report the isolation of Arabidopsis thaliana DREB2A-INTERACTING PROTEIN1 (DRIP1) and DRIP2, C3HC4 RING domain–containing proteins that interact with the DREB2A protein in the nucleus. An in vitro ubiquitination assay showed that they function as E3 ubiquitin ligases and are capable of mediating DREB2A ubiquitination. Overexpression of DRIP1 in Arabidopsis delayed the expression of DREB2A-regulated drought-responsive genes. Drought-inducible gene expression was slightly enhanced in the single T-DNA mutants of drip1-1 and drip2-1. By contrast, significantly enhanced gene expression was revealed in the drip1 drip2 double mutant under dehydration stress. Collectively, these data imply that DRIP1 and DRIP2 function negatively in the response of plants to drought stress. Moreover, overexpression of full-length DREB2A protein was more stable in drip1-1 than in the wild-type background. These results suggest that DRIP1 and DRIP2 act as novel negative regulators in drought-responsive gene expression by targeting DREB2A to 26S proteasome proteolysis.
This article has been cited by other articles:

|
 |

|
 |
 
Y. Osakabe, S. Mizuno, H. Tanaka, K. Maruyama, K. Osakabe, D. Todaka, Y. Fujita, M. Kobayashi, K. Shinozaki, and K. Yamaguchi-Shinozaki
Overproduction of the Membrane-bound Receptor-like Protein Kinase 1, RPK1, Enhances Abiotic Stress Tolerance in Arabidopsis
J. Biol. Chem.,
March 19, 2010;
285(12):
9190 - 9201.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Nishizawa-Yokoi, H. Tainaka, E. Yoshida, M. Tamoi, Y. Yabuta, and S. Shigeoka
The 26S Proteasome Function and Hsp90 Activity Involved in the Regulation of HsfA2 Expression in Response to Oxidative Stress
Plant Cell Physiol.,
March 1, 2010;
51(3):
486 - 496.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Yamada, Y. Osakabe, J. Mizoi, K. Nakashima, Y. Fujita, K. Shinozaki, and K. Yamaguchi-Shinozaki
Functional Analysis of an Arabidopsis thaliana Abiotic Stress-inducible Facilitated Diffusion Transporter for Monosaccharides
J. Biol. Chem.,
January 8, 2010;
285(2):
1138 - 1146.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Fujita, K. Nakashima, T. Yoshida, T. Katagiri, S. Kidokoro, N. Kanamori, T. Umezawa, M. Fujita, K. Maruyama, K. Ishiyama, et al.
Three SnRK2 Protein Kinases are the Main Positive Regulators of Abscisic Acid Signaling in Response to Water Stress in Arabidopsis
Plant Cell Physiol.,
December 1, 2009;
50(12):
2123 - 2132.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Kidokoro, K. Maruyama, K. Nakashima, Y. Imura, Y. Narusaka, Z. K. Shinwari, Y. Osakabe, Y. Fujita, J. Mizoi, K. Shinozaki, et al.
The Phytochrome-Interacting Factor PIF7 Negatively Regulates DREB1 Expression under Circadian Control in Arabidopsis
Plant Physiology,
December 1, 2009;
151(4):
2046 - 2057.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T.-H. Lee, Y.-K. Kim, T. T. M. Pham, S. I. Song, J.-K. Kim, K. Y. Kang, G. An, K.-H. Jung, D. W. Galbraith, M. Kim, et al.
RiceArrayNet: A Database for Correlating Gene Expression from Transcriptome Profiling, and Its Application to the Analysis of Coexpressed Genes in Rice
Plant Physiology,
September 1, 2009;
151(1):
16 - 33.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Maruyama, M. Takeda, S. Kidokoro, K. Yamada, Y. Sakuma, K. Urano, M. Fujita, K. Yoshiwara, S. Matsukura, Y. Morishita, et al.
Metabolic Pathways Involved in Cold Acclimation Identified by Integrated Analysis of Metabolites and Transcripts Regulated by DREB1A and DREB2A
Plant Physiology,
August 1, 2009;
150(4):
1972 - 1980.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Nakashima, Y. Fujita, N. Kanamori, T. Katagiri, T. Umezawa, S. Kidokoro, K. Maruyama, T. Yoshida, K. Ishiyama, M. Kobayashi, et al.
Three Arabidopsis SnRK2 Protein Kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, Involved in ABA Signaling are Essential for the Control of Seed Development and Dormancy
Plant Cell Physiol.,
July 1, 2009;
50(7):
1345 - 1363.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Q. Bu, H. Li, Q. Zhao, H. Jiang, Q. Zhai, J. Zhang, X. Wu, J. Sun, Q. Xie, D. Wang, et al.
The Arabidopsis RING Finger E3 Ligase RHA2a Is a Novel Positive Regulator of Abscisic Acid Signaling during Seed Germination and Early Seedling Development
Plant Physiology,
May 1, 2009;
150(1):
463 - 481.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. K. Lee, S. K. Cho, O. Son, Z. Xu, I. Hwang, and W. T. Kim
Drought Stress-Induced Rma1H1, a RING Membrane-Anchor E3 Ubiquitin Ligase Homolog, Regulates Aquaporin Levels via Ubiquitination in Transgenic Arabidopsis Plants
PLANT CELL,
February 1, 2009;
21(2):
622 - 641.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Nakashima, Y. Ito, and K. Yamaguchi-Shinozaki
Transcriptional Regulatory Networks in Response to Abiotic Stresses in Arabidopsis and Grasses
Plant Physiology,
January 1, 2009;
149(1):
88 - 95.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Mach
Ubiquitin Ligation RINGs Twice: Redundant Control of Plant Processes by E3 Ubiquitin Ligases
PLANT CELL,
June 1, 2008;
20(6):
1424 - 1424.
[Full Text]
[PDF]
|
 |
|
|
|