Plant Cell Advance Online Publication
Published on July 15, 2008; 10.1105/tpc.107.057570
Received December 17, 2007
Returned for revision June 27, 2008
Accepted July 2, 2008
Auxin Regulates Arabidopsis Anther Dehiscence, Pollen Maturation, and Filament Elongation
Valentina Cecchetti 1, Maria Maddalena Altamura 2, Giuseppina Falasca 2, Paolo Costantino 1, and Maura Cardarelli 3*
1 Dipartimento di Genetica e Biologia Molecolare, Istituto Pasteur Fondazione Cenci Bolognetti, Università La Sapienza, 00185 Rome, Italy
2 Dipartimento di Biologia Vegetale, Università La Sapienza, 00185, Rome, Italy
3 Istituto Biologia e Patologia Molecolari, Consiglio Nazionale delle Ricerche, Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, 00185 Rome, Italy
* To whom correspondence should be addressed. E-mail: maura.cardarelli{at}uniroma1.it.
We provide evidence on the localization, synthesis, transport, and effects of auxin on the processes occurring late in Arabidopsis thaliana stamen development: anther dehiscence, pollen maturation, and preanthesis filament elongation. Expression of auxin-sensitive reporter constructs suggests that auxin effects begin in anthers between the end of meiosis and the bilocular stage in the somatic tissues involved in the first step of dehiscence as well as in the microspores and in the junction region between anther and filament. In situ hybridizations of the auxin biosynthetic genes YUC2 and YUC6 suggest that auxin is synthesized in anthers. In agreement with the timing of auxin effects, the TIR1, AFB1, AFB2, and AFB3 auxin receptor-encoding genes are transcribed in anthers only during late stages of development starting at the end of meiosis. We found that in tir1 afb triple and quadruple mutants, anther dehiscence and pollen maturation occur earlier than in the wild type, causing the release of mature pollen grains before the completion of filament elongation. We also assessed the contribution of auxin transport to late stamen developmental processes. Our results suggest that auxin synthesized in anthers plays a major role in coordinating anther dehiscence and pollen maturation, while auxin transport contributes to the independent regulation of preanthesis filament elongation.
This article has been cited by other articles:
|
|
|
|
 
E. P. Thompson, C. Wilkins, V. Demidchik, J. M. Davies, and B. J. Glover
An Arabidopsis flavonoid transporter is required for anther dehiscence and pollen development
J. Exp. Bot.,
January 1, 2010;
61(2):
439 - 451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Sundberg and L. Ostergaard
Distinct and Dynamic Auxin Activities During Reproductive Development
Cold Spring Harb Perspect Biol,
December 1, 2009;
1(6):
a001628 - a001628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2008
J. Exp. Bot.,
June 23, 2009;
(2009)
erp154v1.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Grant-Downton, S. Hafidh, D. Twell, and H. G. Dickinson
Small RNA Pathways Are Present and Functional in the Angiosperm Male Gametophyte
Mol Plant,
May 1, 2009;
2(3):
500 - 512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. A. Wilson and D.-B. Zhang
From Arabidopsis to rice: pathways in pollen development
J. Exp. Bot.,
April 1, 2009;
60(5):
1479 - 1492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hirano, K. Aya, T. Hobo, H. Sakakibara, M. Kojima, R. A. Shim, Y. Hasegawa, M. Ueguchi-Tanaka, and M. Matsuoka
Comprehensive Transcriptome Analysis of Phytohormone Biosynthesis and Signaling Genes in Microspore/Pollen and Tapetum of Rice
Plant Cell Physiol.,
October 1, 2008;
49(10):
1429 - 1450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Eckardt
Auxin Regulation of Late Stamen Development
PLANT CELL,
July 1, 2008;
20(7):
1733 - 1733.
[Full Text]
[PDF]
|
|
|
|
|
