Plant Cell Advance Online Publication
Published on July 25, 2008; 10.1105/tpc.108.059139
OPEN ACCESS ARTICLE
Received March 1, 2008
Returned for revision June 4, 2008
Accepted July 4, 2008
The EPIP Peptide of INFLORESCENCE DEFICIENT IN ABSCISSION Is Sufficient to Induce Abscission in Arabidopsis through the Receptor-Like Kinases HAESA and HAESA-LIKE2
Grethe-Elisabeth Stenvik 1, Nora M. Tandstad 1, Yongfeng Guo 2, Chun-Lin Shi 1, Wenche Kristiansen 1, Asbjørn Holmgren 1, Steven E. Clark 2, Reidunn B. Aalen 1*, and Melinka A. Butenko 1
1 Department of Molecular Biosciences, University of Oslo, N-0316 Oslo, Norway
2 Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048
* To whom correspondence should be addressed. E-mail: reidunn.aalen{at}imbv.uio.no.
In Arabidopsis thaliana, the final step of floral organ abscission is regulated by INFLORESCENCE DEFICIENT IN ABSCISSION (IDA): ida mutants fail to abscise floral organs, and plants overexpressing IDA display earlier abscission. We show that five IDA-LIKE (IDL) genes are expressed in different tissues, but plants overexpressing these genes have phenotypes similar to IDA-overexpressing plants, suggesting functional redundancy. IDA/IDL proteins have N-terminal signal peptides and a C-terminal conserved motif (extended PIP [EPIP]) at the C terminus (EPIP-C). IDA can, similar to CLAVATA3, be processed by an activity from cauliflower meristems. The EPIP-C of IDA and IDL1 replaced IDA function in vivo, when the signal peptide was present. In addition, synthetic IDA and IDL1 EPIP peptides rescued ida and induced early floral abscission in wild-type flowers. The EPIP-C of the other IDL proteins could partially substitute for IDA function. Similarly to ida, a double mutant between the receptor-like kinases (RLKs) HAESA (HAE) and HAESA-LIKE2 (HSL2) displays nonabscising flowers. Neither overexpression of IDA nor synthetic EPIP or EPIP-C peptides could rescue the hae hsl2 abscission deficiency. We propose that IDA and the IDL proteins constitute a family of putative ligands that act through RLKs to regulate different events during plant development.
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