Plant Cell Huazhong Agricultural University
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Cell Advance Online Publication
Published on April 3, 2009; 10.1105/tpc.109.066159

This Article
Right arrow Full Text - TPC Advance Online Pub. (PDF)
Right arrow Supplemental Data
Right arrow Author Profile
Right arrow All Versions of this Article:
21/4/1166    most recent
tpc.109.066159v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ueki, N.
Right arrow Articles by Nishii, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ueki, N.
Right arrow Articles by Nishii, I.
Agricola
Right arrow Articles by Ueki, N.
Right arrow Articles by Nishii, I.

Received February 10, 2009
Returned for revision March 11, 2009
Accepted March 18, 2009

Controlled Enlargement of the Glycoprotein Vesicle Surrounding a Volvox Embryo Requires the InvB Nucleotide-Sugar Transporter and Is Required for Normal Morphogenesis

Noriko Ueki 1* and Ichiro Nishii 1

1 Nishii Initiative Research Unit, RIKEN Advanced Science Institute, Wako-shi, 351-0198, Japan

* To whom correspondence should be addressed. E-mail: noriko.ueki{at}uni-bielefeld.de.

Here, we report our analysis of a mutant of Volvox carteri, InvB, whose embryos fail to execute inversion, the process in which each Volvox embryo normally turns itself inside-out at the end of embryogenesis, thereby achieving the adult configuration. The invB gene encodes a nucleotide-sugar transporter that exhibits GDP-mannose transport activity when expressed in yeast. In wild-type embryos, the invB transcript is maximally abundant before and during inversion. A mannoside probe (fluorescent concanavalin A) stains the glycoprotein-rich gonidial vesicle (GV) surrounding wild-type embryos much more strongly than it stains the GV surrounding InvB embryos. Direct measurements revealed that throughout embryogenesis the GV surrounding a wild-type embryo increases in size much more than the GV surrounding an InvB embryo does, and the fully cleaved InvB embryo is much more tightly packed within its GV than a wild-type embryo is. To test the hypothesis that the restraint imposed by a smaller than normal GV directly causes the inversion defect in the mutant, we released InvB embryos from their GVs microsurgically. The resulting embryos inverted normally, demonstrating that controlled enlargement of the GV, by a process in which requires the InvB nucleotide-sugar transporter, is essential to provide the embryo sufficient space to complete inversion.




This article has been cited by other articles:


Home page
 Plant CellHome page
N. A. Eckardt
A Volvox Inversionless Mutant Highlights the Importance of the Extracellular Matrix in Morphogenesis
PLANT CELL, April 1, 2009; 21(4): 1029 - 1029.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2009 by the American Society of Plant Biologists