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Abstract
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ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters.

E Lam, N H Chua
E Lam
Laboratory of Plant Molecular Biology, Rockefeller University, New York 10021-6399.
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N H Chua
Laboratory of Plant Molecular Biology, Rockefeller University, New York 10021-6399.
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Published December 1989. DOI: https://doi.org/10.1105/tpc.1.12.1147

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  • Copyright © 1989 by American Society of Plant Biologists

Abstract

We have used nuclear extracts prepared from tobacco leaf tissue to characterize a factor binding site, designated as-2 (activating sequence-2), at the -100 region of the cauliflower mosaic virus 35S promoter. The activity of this factor, called ASF-2 (activating sequence factor-2), is not detected in tobacco root extracts. as-2 includes two GT motifs with sequence homology to the SV40 enhancer core A element and the Box II element of pea rbcS. Nevertheless, oligomers of these sequence elements do not compete for ASF-2 binding in gel retardation assays, indicating that the GT motifs may not be involved. Methylation interference studies identify two guanines (G93 and G98) that are required for interaction with ASF-2. Sequences surrounding these two critical guanines display homologies to a GATA repeat conserved among several light-responsive promoters. One such sequence from a petunia Cab promoter is able to compete with as-2 for factor binding. In transgenic plants, a tetramer of as-2 is able to confer leaf expression when fused 5' to the -90 derivative of the 35S promoter. The expression is not dependent on light and, thus, the as-2 tetramer does not function as a light-responsive element in this context. Histochemical localization of the reporter gene product suggests that the as-2 tetramer directs expression in trichomes, vascular elements, and epidermal and mesophyll cells.

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ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters.
E Lam, N H Chua
The Plant Cell Dec 1989, 1 (12) 1147-1156; DOI: 10.1105/tpc.1.12.1147

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ASF-2: a factor that binds to the cauliflower mosaic virus 35S promoter and a conserved GATA motif in Cab promoters.
E Lam, N H Chua
The Plant Cell Dec 1989, 1 (12) 1147-1156; DOI: 10.1105/tpc.1.12.1147
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The Plant Cell
Vol. 1, Issue 12
Dec 1989
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