Article Figures & Data
Figures
- Figure 1.
Flowering Phenotype of Lines Described in This Study.
(A) Homozygous FRI-SF2 in the Col background (left) and a fast-neutron-induced flc allele in the same genetic background (right).
(B) Homozygous FRI-SF2 in the Ler background (left) and introduction of a genomic clone containing a late-flowering allele of FLC into a FRI-SF2 in Ler (right).
(C) Ler wild type (left) and introduction of constitutively expressed FLC into the Ler background (right).
- Figure 2.
Cloning and Characterization of the FLC Gene.
(A) Summary of the positional cloning of FLC, genomic structure, and positions of mutations. Exons are represented by filled boxes, and lengths are give above; introns are represented by open boxes, and lengths are given below. Asterisks denote the positions of mutations.
(B) The predicted amino acid sequence of FLC, with the MADS, I, and K domains indicated.
- Figure 3.
Expression of FLC.
(A) Expression of FLC in Col wild type (Col), FRI-SF2 in Col (FRI), a fast-neutron allele of fri in Col (fri), Ws wild type, and the ld-3 mutant in the Ws background.
(B) Time course of FLC expression in RNA prepared from shoots. Time is measured as days after germination (DAG).
(C) Expression of FLC in various organs.
(D) Suppression of FLC expression by 30 days after cold treatment. Plants were grown for 10 days at 22°C before harvesting. Blots were probed with 18S rDNA as a control for loading.
- Figure 4.
Model for the Interaction of FLC, FRI, LD, and Vernalization in the Regulation of Flowering in the Autonomous Pathway.
Boldface lines indicate interactions with FLC, and dashed lines indicate other effects that occur in the absence of FLC activity.
Tables
Line Leaf Number at Floweringa Description Col 12.2 (.40)b Col wild type (fri-Col/fri-Col; FLC-Col/FLC-Col) FRI-SF2 in Col 67.8 (4.7) Homozygous for the SF2 allele of FRI in the Col background, derived from the eighth backcrossc (FRI-SF2/FRI-SF2; FLC-Col/FLC-Col) flc-2 in FRI-SF2 in Col 11.8 (.75) Fast-neutron flc allele in FRI-SF2 in the Col background (FRI-SF2/FRI-SF2; flc-2/flc-2) flc-3 in FRI-SF2 in Col 11.3 (.47) Fast-neutron flc allele in FRI-SF2 in the Col background (FRI-SF2/FRI-SF2; flc-3/flc-3) flc-4 in FRI-SF2 in Col 11.5 (.50) Fast-neutron flc allele in FRI-SF2 in the Col background (FRI-SF2/FRI-SF2; flc-4/flc-4) FRI-SF2 in Ler 12.2 (.40) Homozygous for the SF2 allele of FRI in the Ler background, derived from the tenth backcrossc (FRI-SF2/FRI-SF2; flc-Ler/flc-Ler) FN235 11.8 (.63) Fast-neutron fri allele isolated from FRI-SF2 in the Col background (fri/fri; FLC-Col/FLC-Col)
