Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Research ArticleResearch Article
You have accessRestricted Access

Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis

Justin O. Borevitz, Yiji Xia, Jack Blount, Richard A. Dixon, Chris Lamb
Justin O. Borevitz
a Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yiji Xia
a Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037
b Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jack Blount
b Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Richard A. Dixon
b Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chris Lamb
a Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: chris.lamb@bbsrc.ac.uk

Published December 2000. DOI: https://doi.org/10.1105/tpc.12.12.2383

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • © 2000 American Society of Plant Physiologists

Abstract

Plants produce a wide array of natural products, many of which are likely to be useful bioactive structures. Unfortunately, these complex natural products usually occur at very low abundance and with restricted tissue distribution, thereby hindering their evaluation. Here, we report a novel approach for enhancing the accumulation of natural products based on activation tagging by Agrobacterium-mediated transformation with a T-DNA that carries cauliflower mosaic virus 35S enhancer sequences at its right border. Among ∼5000 Arabidopsis activation-tagged lines, we found a plant that exhibited intense purple pigmentation in many vegetative organs throughout development. This upregulation of pigmentation reflected a dominant mutation that resulted in massive activation of phenylpropanoid biosynthetic genes and enhanced accumulation of lignin, hydroxycinnamic acid esters, and flavonoids, including various anthocyanins that were responsible for the purple color. These phenotypes, caused by insertion of the viral enhancer sequences adjacent to an MYB transcription factor gene, indicate that activation tagging can overcome the stringent genetic controls regulating the accumulation of specific natural products during plant development. Our findings suggest a functional genomics approach to the biotechnological evaluation of phytochemical biodiversity through the generation of massively enriched tissue sources for drug screening and for isolating underlying regulatory and biosynthetic genes.

  • Received June 6, 2000.
  • Accepted October 3, 2000.
  • Published December 1, 2000.
View Full Text
PreviousNext
Back to top

Table of Contents

Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
Justin O. Borevitz, Yiji Xia, Jack Blount, Richard A. Dixon, Chris Lamb
The Plant Cell Dec 2000, 12 (12) 2383-2393; DOI: 10.1105/tpc.12.12.2383

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
Justin O. Borevitz, Yiji Xia, Jack Blount, Richard A. Dixon, Chris Lamb
The Plant Cell Dec 2000, 12 (12) 2383-2393; DOI: 10.1105/tpc.12.12.2383
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • METHODS
    • Acknowledgments
    • Footnotes
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell Online: 12 (12)
The Plant Cell
Vol. 12, Issue 12
Dec 2000
  • Table of Contents
  • About the Cover
  • Index by author
View this article with LENS

More in this TOC Section

  • Abscisic Acid-Triggered Persulfidation of the Cys Protease ATG4 Mediates Regulation of Autophagy by Sulfide
  • Temporal Regulation of the Metabolome and Proteome in Photosynthetic and Photorespiratory Pathways Contributes to Maize Heterosis
  • Chloroplast Chaperonin-Mediated Targeting of a Thylakoid Membrane Protein
Show more RESEARCH ARTICLES

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire