Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Research ArticleResearch Article
You have accessRestricted Access

Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint

Chizuko Yamamuro, Yoshihisa Ihara, Xiong Wu, Takahiro Noguchi, Shozo Fujioka, Suguru Takatsuto, Motoyuki Ashikari, Hidemi Kitano, Makoto Matsuoka
Chizuko Yamamuro
a Nagoya University, BioScience Center, Chikusa, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoshihisa Ihara
b School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiong Wu
b School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takahiro Noguchi
c Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shozo Fujioka
c Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Suguru Takatsuto
d Department of Chemistry, Joetsu University of Education, Joetsu, Niigata 943-8512, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Motoyuki Ashikari
a Nagoya University, BioScience Center, Chikusa, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hidemi Kitano
b School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Makoto Matsuoka
a Nagoya University, BioScience Center, Chikusa, Nagoya 464-8601, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: j45751a@nucc.cc.nagoya-u.ac.jp

Published September 2000. DOI: https://doi.org/10.1105/tpc.12.9.1591

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Phenotype of the d61 Mutants.

    (A) Schematic representation of the various elongation patterns of internodes in the wild type (N) and various rice dwarf mutants (dn-, dm-, d6-, nl- and sh-types), adapted from Takeda (1977).

    (B) Gross morphology of a wild-type plant (left); d61-1 mutant (center), a weak allele; and d61-2 mutant (right), a strong allele.

    (C) Elongation pattern of internodes. The wild-type plant (left) shows the N-type of the elongation pattern, whereas the d61-1 (center) and d61-2 (right) mutants show typical dm- and d6-type patterns, respectively. The number of each internode is indicated.

    (D) Panicle structure. The wild-type plant (left) has a short panicle; the d61-1 (center) and d61-2 (right) mutants have longer panicles. The arrows indicate the nodes.

    (E) Leaf morphology. The leaf of the wild-type plant (left) is bent at the lamina joint indicated by the white arrow, whereas the leaves of d61-1 (center) and d61-2 (right) mutants are more erect.

    (F) Leaf sheath morphology. The leaf sheath in the d61-1 (center) and d61-2 (right) mutants is shorter than in the wild-type plant (left).

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Structure of Well-Developed Internodes from Wild-Type and d61-2 Rice Plants and Orientation of Microtubules in Elongating Cells in the First Internode of Wild-Type and d61-2 Plants.

    (A) and (B) Longitudinal sections of the first internode from the wild type and d61-2, respectively.

    (C) and (D) Longitudinal sections of the second internode from the wild type and d61-2, respectively.

    (E) and (F) Longitudinal sections of the third internode from the wild type and d61-2, respectively.

    (G) and (H) Longitudinal sections of the fourth internode from the wild type and d61-2, respectively.

    (I) and (J) Immunofluorescence images of the microtubule arrangement in internodal parenchyma cells of the first internode from the wild type and d61-2, respectively.

    (K) and (L) Schematic presentation of the microtubule arrangement in internodal parenchyma cells of the first internode from the wild type and d61-2, respectively.

    Bars in (A) to (H) = 100 μm; bars in (I) to (L) = 50 μm.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Response of Seedlings to BL.

    (A) Seeds of the wild type (left) and the dwarf mutants d61-1 (center) and d61-2 (right) were germinated on agar plates in the presence (+) or absence (–) of 1 μM BL. Seedlings were examined 1 day after germination.

    (B) Effect of BL on the coleoptile and root elongation in wild-type and d61 seedlings. The plants were germinated in the same conditions as (A) with the indicated concentration of BL. Data presented are the means of results from five plants. Bars indicate sd.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Effect of BL on the Degree of Inclination of Etiolated Leaf Lamina in Wild-Type and d61 Plants.

    (A) Typical response of the second leaf lamina joint from wild-type, d61-1, and d61-2 plants to BL at 10–3 μg mL–1.

    (B) The dose response to BL of the bending angle in the wild type and d61 mutants. Data presented are the means of results from six plants. Bars indicate sd.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Endogenous BRs in Wild-Type and d61-2 Rice Plants.

    The amounts (ng g–1 fresh weight) of BL and its biosynthetic precursors in d61-2 (upper) and wild-type (lower) rice plants are shown. ND, not detected.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    DET Phenotype of the Wild Type, d61, and Two Gibberellin-Deficient Rice Mutants (d18 and d35) in the Dark.

    The left- and right-hand seedlings of each pair were grown for 2 weeks in the light and the dark, respectively. Arrows indicate the nodes and arrowheads indicate the mesocotyls.

  • Figure 7.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 7.

    Length of the Second Lower Internodes of the Wild Type and Mutants Grown in the Dark.

    Data presented are the means of the results from five plants. Bars indicate sd.

  • Figure 8.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 8.

    Deduced Amino Acid Sequences of OsBRI1 and Arabidopsis BRI1.

    Identical residues are shaded. The underlined regions indicate (1) a putative signal peptide, (2) a leucine zipper motif, (3) N-side, and (4) C-side of a cysteine pair.

  • Figure 9.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 9.

    Complementation and Antisense Phenotype of OsBRI1.

    (A) d61 plant with the wild-type OsBRI1gene (left) and d61 plant introduced with a vector only (right).

    (B) Dwarf phenotype of OsBRI1 antisense plants with intermediate and severe phenotypes (right) in comparison with a wild-type plant (left).

    (C) Close-up view of OsBRI1 antisense plants with the severe phenotype.

    (D) Naked culm internodes of transgenic plant. From left to right, wild-type plant with normal elongation pattern of internodes, and OsBRI1 antisense plants with the dm, dm–d6, and d6 phenotypes, respectively.

    (E) Abnormal leaf morphology of an OsBRI1 antisense plant with a severe phenotype, showing lack of developed sheath organs.

    (F) Panicle morphology in wild-type (left) and OsBRI1 antisense plants with the mild (center) and intermediate (right) phenotypes. The arrows indicate the nodes.

    (G) Leaf morphology of wild-type (left) and OsBRI1 antisense plants with mild phenotype (right), showing the erect leaves in the latter.

    Bar in (C) = 5 cm; bar in (E) = 10 cm; bar in (F) = 2 cm.

  • Figure 10.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 10.

    Expression Pattern of OsBRI1 in Various Organs.

    Total RNA (10 μg) from various organs of wild-type plants was probed by hybridization with an OsBRI1 cDNA clone.

    (A) Organ-specific expression of OsBRI1. Lane 1, leaf blade; lane 2, leaf sheath; lane 3, developed flower; lane 4, rachis; lane 5, shoot apex; lane 6, root; and lane 7, seed.

    (B) Region-specific expression of OsBRI1 in developing first internodes. Lane 1, node; lane 2, divisional zone; lane 3, elongation zone; and lane 4, elongated zone.

    (C) Differential expression of OsBRI1 in each elongating internode. Lanes 1 to 4, the divisional and elongation zones of the first to fourth internodes, respectively, at the actively elongating stage for each internode; lane 5, the unelongated stem at the vegetative phase.

    (D) Light-dependent and BL-dependent expression of OsBRI1 (upper panel). Rice seedlings were grown for 10 days in the light (lanes 1 and 2) or dark (lanes 3 and 4) on agar plates in the presence (lanes 2 and 4) or absence (lanes 1 and 3) of 1 μM BL. The middle panel shows the amount of the actin mRNA probed with an expressed sequence tag clone, S14002, from Rice Genome Project as a control.

Tables

  • Figures
    • View popup
    Table 1.

    Phenotype of OsBRI1 Antisense Plants

    Transgenic PlantErect LeavesInternode Elongation Patterna
      1Yesdm
      2YesSevere dwarfb
      3Yesdm
      4YesSevere dwarf
      5YesMixed dm–d6
      6YesSevere dwarf
      7Nodn
      8Yesdn
      9Nodn
    10YesSevere dwarf
    11YesMixed dm–d6
    12Yesdn
    13YesMixed dm–d6
    14YesSevere dwarf
    15Yesdn
    16Yesdn
    17YesSevere dwarf
    18Yesdm
    19YesMixed dm–d6
    20YesMixed dm–d6
    • ↵a The elongation pattern of rice internode was represented in Figure 1A.

    • ↵b Plants with the severe dwarf only formed abnormal leaves without internode elongation (see Figure 9C).

PreviousNext
Back to top

Table of Contents

Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint
Chizuko Yamamuro, Yoshihisa Ihara, Xiong Wu, Takahiro Noguchi, Shozo Fujioka, Suguru Takatsuto, Motoyuki Ashikari, Hidemi Kitano, Makoto Matsuoka
The Plant Cell Sep 2000, 12 (9) 1591-1605; DOI: 10.1105/tpc.12.9.1591

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Loss of Function of a Rice brassinosteroid insensitive1 Homolog Prevents Internode Elongation and Bending of the Lamina Joint
Chizuko Yamamuro, Yoshihisa Ihara, Xiong Wu, Takahiro Noguchi, Shozo Fujioka, Suguru Takatsuto, Motoyuki Ashikari, Hidemi Kitano, Makoto Matsuoka
The Plant Cell Sep 2000, 12 (9) 1591-1605; DOI: 10.1105/tpc.12.9.1591
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • METHODS
    • Acknowledgments
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell Online: 12 (9)
The Plant Cell
Vol. 12, Issue 9
Sep 2000
  • Table of Contents
  • About the Cover
  • Index by author
View this article with LENS

More in this TOC Section

  • Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana
  • Abscisic Acid-Triggered Persulfidation of the Cys Protease ATG4 Mediates Regulation of Autophagy by Sulfide
  • Temporal Regulation of the Metabolome and Proteome in Photosynthetic and Photorespiratory Pathways Contributes to Maize Heterosis
Show more RESEARCH ARTICLES

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire