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OtherGenomics Article
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Growth Stage–Based Phenotypic Analysis of Arabidopsis

A Model for High Throughput Functional Genomics in Plants

Douglas C. Boyes, Adel M. Zayed, Robert Ascenzi, Amy J. McCaskill, Neil E. Hoffman, Keith R. Davis, Jörn Görlach
Douglas C. Boyes
Department of Plant Research, Paradigm Genetics, Inc., Research Triangle Park, North Carolina 27709
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Adel M. Zayed
Department of Plant Research, Paradigm Genetics, Inc., Research Triangle Park, North Carolina 27709
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Robert Ascenzi
Department of Plant Research, Paradigm Genetics, Inc., Research Triangle Park, North Carolina 27709
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Amy J. McCaskill
Department of Plant Research, Paradigm Genetics, Inc., Research Triangle Park, North Carolina 27709
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Neil E. Hoffman
Department of Plant Research, Paradigm Genetics, Inc., Research Triangle Park, North Carolina 27709
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Keith R. Davis
Department of Plant Research, Paradigm Genetics, Inc., Research Triangle Park, North Carolina 27709
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Jörn Görlach
Department of Plant Research, Paradigm Genetics, Inc., Research Triangle Park, North Carolina 27709
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Published July 2001. DOI: https://doi.org/10.1105/TPC.010011

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Abstract

With the completion of the Arabidopsis genome sequencing project, the next major challenge is the large-scale determination of gene function. As a model organism for agricultural biotechnology, Arabidopsis presents the opportunity to provide key insights into the way that gene function can affect commercial crop production. In an attempt to aid in the rapid discovery of gene function, we have established a high throughput phenotypic analysis process based on a series of defined growth stages that serve both as developmental landmarks and as triggers for the collection of morphological data. The data collection process has been divided into two complementary platforms to ensure the capture of detailed data describing Arabidopsis growth and development over the entire life of the plant. The first platform characterizes early seedling growth on vertical plates for a period of 2 weeks. The second platform consists of an extensive set of measurements from plants grown on soil for a period of ∼2 months. When combined with parallel processes for metabolic and gene expression profiling, these platforms constitute a core technology in the high throughput determination of gene function. We present here analyses of the development of wild-type Columbia (Col-0) plants and selected mutants to illustrate a framework methodology that can be used to identify and interpret phenotypic differences in plants resulting from genetic variation and/or environmental stress.

  • Received January 9, 2001.
  • Accepted May 17, 2001.
  • Published July 1, 2001.
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Growth Stage–Based Phenotypic Analysis of Arabidopsis
Douglas C. Boyes, Adel M. Zayed, Robert Ascenzi, Amy J. McCaskill, Neil E. Hoffman, Keith R. Davis, Jörn Görlach
The Plant Cell Jul 2001, 13 (7) 1499-1510; DOI: 10.1105/TPC.010011

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Growth Stage–Based Phenotypic Analysis of Arabidopsis
Douglas C. Boyes, Adel M. Zayed, Robert Ascenzi, Amy J. McCaskill, Neil E. Hoffman, Keith R. Davis, Jörn Görlach
The Plant Cell Jul 2001, 13 (7) 1499-1510; DOI: 10.1105/TPC.010011
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The Plant Cell Online: 13 (7)
The Plant Cell
Vol. 13, Issue 7
Jul 2001
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More in this TOC Section

  • Genome-Wide Analysis of NBS-LRR–Encoding Genes in Arabidopsis
  • Laser-Capture Microdissection, a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize
  • A High-Throughput Arabidopsis Reverse Genetics System
Show more GENOMICS ARTICLE

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