Endocytosis proceeds by the invagination of vesicles from the plasma membrane and their budding into the cytoplasm. Endocytosed membrane is transported through a series of organelles before its delivery to the vacuole. In the paper by Emans et al. (71), live cell imaging was used to follow the uptake of a fluorescent marker (FM 1-43) from the plasma membrane and its delivery to the vacuole in tobacco suspension cells. Uptake was temperature dependent and sensitive to two modulators of membrane traffic, Wortmannin and Brefeldin A. The cover image shows a time-lapse series of three-dimensional confocal images of the vacuole and endocytic vesicles after FM 1-43 uptake, demonstrating vesicle and trans-vacuolar strand movement. Each panel corresponds to a 7 µm Z section and are images taken at 1 min intervals from the top left to bottom right.