Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
OtherGENOMICS HIGHLIGHT
You have accessRestricted Access

Chlamydomonas Chloroplast Genome and Transcriptome Analysis

Nancy A. Eckardt
Nancy A. Eckardt
Roles: News and Reviews Editor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published November 2002. DOI: https://doi.org/10.1105/tpc.141170

  • Article
  • Figures & Data
  • Info & Metrics
Loading
  • American Society of Plant Biologists

Chlamydomonas reinhardtii is a unicellular green alga that is widely used as a model system in the study of photosynthesis and other aspects of cell biology. This issue of The Plant Cell includes two reports on Chlamydomonas genomics that represent important landmarks in plant cell biology (Figure 1) . Maul et al. (pages 2659–2679) present the complete sequence and a comparative analysis of the Chlamydomonas chloroplast genome, and in a companion article, Lilly et al. (pages 2681–2706) examine the Chlamydomonas chloroplast and mitochondrial transcriptomes under a variety of environmental conditions.

Figure 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1.

The Genome Map of Chlamydomonas, Showing the Major Classes of Genes, Superimposed on a False-Color Image of a Chloroplast/Mitochondria Transcriptome DNA Microarray and an Epifluorescence Microscopy Image of Cells Expressing Green Fluorescent Protein in the Chloroplast.

In this issue, Maul et al. and Lilly et al. present bioinformatic and functional analyses of the completed Chlamydomonas plastid genome. (GFP microscopic image courtesy of Yutaka Komine.)

The sequencing of the Chlamydomonas chloroplast genome and genome analysis presented by Maul et al. (2002) is the result of a collaborative effort between researchers at three institutions (Jude Maul, Jason Lilly, and David Stern at the Boyce Thompson Institute, Cornell University, Ithaca, NY; Liying Cui, Claude dePamphilis, and Webb Miller at Pennsylvania State University, University Park, PA; and Elizabeth Harris at Duke University, Durham, NC). In addition to a complete annotation of the genome, Maul et al. (2002) performed a comparative analysis of the Chlamydomonas chloroplast with 13 other fully sequenced plastid genomes and 2 cyanobacterial genomes. A surprising finding was that >20% of the Chlamydomonas chloroplast genome consists of repetitive DNA that includes numerous classes of short dispersed repeats. The only other sequenced chloroplast genome found to share this feature was that of another chlorophyte, Chlorella vulgaris. The Chlamydomonas/Chlo-rella lineage also was found to be characterized by an accelerated rate of gene loss compared with other chloroplast lineages. This analysis reveals a highly dynamic and unusual chloroplast genome and a wealth of new data that will benefit studies of functional, structural, and com-parative genomics.

In the second article, Lilly, Maul, and Stern conducted global gene expression analysis of the Chlamydomonas chloroplast and mitochondrial transcriptomes under a variety of environmental conditions. The results showed strong organellar transcriptional changes in response to abiotic stresses such as phosphate and sulfur limitation, including changes in the expression of genes known to be involved in post-transcriptional processing and regulation (e.g., RNA processing and translation initiation). The results sug-gest that chloroplast transcript abundance is fully integrated into the cell's signaling network. Future work using the tools and approach described by Lilly et al. (2002) will provide valuable insights into the coordination of organellar and nuclear gene expression.

More information on Chlamydomonas genomics, as well as access to databases discussed in these articles, can be found at The Chlamydomonas Genetics Center World Wide Web site at http://www.biology.duke.edu/chlamy/. An interactive site featuring the Chlamydomonas chloroplast genome and relevant resources can be found at http://bti.cornell.edu/bti2/chlamyweb/.

References

  1. ↵
    Lilly, J.W., Maul, J.E., and Stern, D.B. (2002). The Chlamydomonas reinhardtii organellar genomes respond transcriptionally and post-transcriptionally to abiotic stimuli. Plant Cell 14, 2681–2706.
    OpenUrlAbstract/FREE Full Text
  2. ↵
    Maul, J.E., Lilly, J.W., Cui, L., dePamphilis, C.W., Miller, W., Harris, E.H., and Stern, D.B. (2002). The Chlamydomonas reinhardtii plastid chromosome: Islands of genes in a sea of repeats. Plant Cell 14, 2659–2679.
    OpenUrlAbstract/FREE Full Text
PreviousNext
Back to top

Table of Contents

Print
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Chlamydomonas Chloroplast Genome and Transcriptome Analysis
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Chlamydomonas Chloroplast Genome and Transcriptome Analysis
Nancy A. Eckardt
The Plant Cell Nov 2002, 14 (11) 2657-2658; DOI: 10.1105/tpc.141170

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Chlamydomonas Chloroplast Genome and Transcriptome Analysis
Nancy A. Eckardt
The Plant Cell Nov 2002, 14 (11) 2657-2658; DOI: 10.1105/tpc.141170
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • References
  • Figures & Data
  • Info & Metrics

In this issue

The Plant Cell Online: 14 (11)
The Plant Cell
Vol. 14, Issue 11
Nov 2002
  • Table of Contents
  • About the Cover
  • Index by author
View this article with LENS

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire