Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • Info for
    • Instructions for Authors
    • Submit a Manuscript
    • Advertisers
    • Librarians
    • Subscribers
  • About
    • Editorial Board and Staff
    • About the Journal
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • Info for
    • Instructions for Authors
    • Submit a Manuscript
    • Advertisers
    • Librarians
    • Subscribers
  • About
    • Editorial Board and Staff
    • About the Journal
  • More
    • Alerts
    • Contact Us
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Research ArticleResearch Article
You have accessRestricted Access

Enzymes of Glycolysis Are Functionally Associated with the Mitochondrion in Arabidopsis Cells

Philippe Giegé, Joshua L. Heazlewood, Ute Roessner-Tunali, A. Harvey Millar, Alisdair R. Fernie, Christopher J. Leaver, Lee J. Sweetlove
Philippe Giegé
Institut de Biologie Moléculaire des Plantes, Strasbourg 67084, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joshua L. Heazlewood
School of BioMedical and Chemical Sciences, University of Western Australia, Crawley 6009, Western Australia, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ute Roessner-Tunali
Department of Lothar Willmitzer, Max-Planck-Institute for Molecular Plant Physiology, 14476 Golm, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. Harvey Millar
School of BioMedical and Chemical Sciences, University of Western Australia, Crawley 6009, Western Australia, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alisdair R. Fernie
Department of Lothar Willmitzer, Max-Planck-Institute for Molecular Plant Physiology, 14476 Golm, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher J. Leaver
Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lee J. Sweetlove
Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published September 2003. DOI: https://doi.org/10.1105/tpc.012500

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • American Society of Plant Biologists

Abstract

Mitochondria fulfill a wide range of metabolic functions in addition to the synthesis of ATP and contain a diverse array of proteins to perform these functions. Here, we present the unexpected discovery of the presence of the enzymes of glycolysis in a mitochondrial fraction of Arabidopsis cells. Proteomic analyses of this mitochondrial fraction revealed the presence of 7 of the 10 enzymes that constitute the glycolytic pathway. Four of these enzymes (glyceraldehyde-3-P dehydrogenase, aldolase, phosphoglycerate mutase, and enolase) were also identified in an intermembrane space/outer mitochondrial membrane fraction. Enzyme activity assays confirmed that the entire glycolytic pathway was present in preparations of isolated Arabidopsis mitochondria, and the sensitivity of these activities to protease treatments indicated that the glycolytic enzymes are present on the outside of the mitochondrion. The association of glycolytic enzymes with mitochondria was confirmed in vivo by the expression of enolase– and aldolase–yellow fluorescent protein fusions in Arabidopsis protoplasts. The yellow fluorescent protein fluorescence signal showed that these two fusion proteins are present throughout the cytosol but are also concentrated in punctate regions that colocalized with the mitochondrion-specific probe Mitotracker Red. Furthermore, when supplied with appropriate cofactors, isolated, intact mitochondria were capable of the metabolism of 13C-glucose to 13C-labeled intermediates of the trichloroacetic acid cycle, suggesting that the complete glycolytic sequence is present and active in this subcellular fraction. On the basis of these data, we propose that the entire glycolytic pathway is associated with plant mitochondria by attachment to the cytosolic face of the outer mitochondrial membrane and that this microcompartmentation of glycolysis allows pyruvate to be provided directly to the mitochondrion, where it is used as a respiratory substrate.

  • Received March 31, 2003.
  • Accepted June 24, 2003.
  • Published August 8, 2003.
View Full Text
PreviousNext
Back to top

Table of Contents

Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Enzymes of Glycolysis Are Functionally Associated with the Mitochondrion in Arabidopsis Cells
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
Citation Tools
Enzymes of Glycolysis Are Functionally Associated with the Mitochondrion in Arabidopsis Cells
Philippe Giegé, Joshua L. Heazlewood, Ute Roessner-Tunali, A. Harvey Millar, Alisdair R. Fernie, Christopher J. Leaver, Lee J. Sweetlove
The Plant Cell Sep 2003, 15 (9) 2140-2151; DOI: 10.1105/tpc.012500

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Enzymes of Glycolysis Are Functionally Associated with the Mitochondrion in Arabidopsis Cells
Philippe Giegé, Joshua L. Heazlewood, Ute Roessner-Tunali, A. Harvey Millar, Alisdair R. Fernie, Christopher J. Leaver, Lee J. Sweetlove
The Plant Cell Sep 2003, 15 (9) 2140-2151; DOI: 10.1105/tpc.012500
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • METHODS
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell Online: 15 (9)
The Plant Cell
Vol. 15, Issue 9
Sep 2003
  • Table of Contents
  • About the Cover
  • Index by author
View this article with LENS

More in this TOC Section

  • RecQ Helicases Function in Development, DNA Repair, and Gene Targeting in Physcomitrella patens
  • LLM-Domain B-GATA Transcription Factors Play Multifaceted Roles in Controlling Greening in Arabidopsis
  • A Plant Phytosulfokine Peptide Initiates Auxin-Dependent Immunity through Cytosolic Ca2+ Signaling in Tomato
Show more Research Article

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2018 by The American Society of Plant Biologists

Powered by HighWire