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An Arabidopsis Endo-1,4-β-d-Glucanase Involved in Cellulose Synthesis Undergoes Regulated Intracellular Cycling

Stéphanie Robert, Adeline Bichet, Olivier Grandjean, Daniel Kierzkowski, Béatrice Satiat-Jeunemaître, Sandra Pelletier, Marie-Theres Hauser, Herman Höfte, Samantha Vernhettes
Stéphanie Robert
aLaboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
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Adeline Bichet
aLaboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
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Olivier Grandjean
bLaboratoire Commun de Cytologie, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
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Daniel Kierzkowski
aLaboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
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Béatrice Satiat-Jeunemaître
cDynamique de la Compartimentation Cellulaire, Institut des Sciences du Végétal, Centre National de la Recherche Scientifique Unité Propre de Recherche 2355, 91198 Gif sur Yvette Cedex, France
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Sandra Pelletier
aLaboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
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Marie-Theres Hauser
dInstitute of Applied Genetics and Cell Biology, BOKU, University of Natural Resources and Applied Life Science, A-1190 Vienna, Austria
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Herman Höfte
aLaboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
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Samantha Vernhettes
aLaboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
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Published December 2005. DOI: https://doi.org/10.1105/tpc.105.036228

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Abstract

The synthesis of cellulose microfibrils requires the presence of a membrane-bound endo-1,4-β-d-glucanase, KORRIGAN1 (KOR1). Although the exact biochemical role of KOR1 in cellulose synthesis is unknown, we used the protein as a marker to explore the potential involvement of subcellular transport processes in cellulose synthesis. Using immunofluorescence and a green fluorescent protein (GFP)–KOR1 fusion that complemented the phenotype conferred by the kor1-1 mutant, we investigated the distribution of KOR1 in epidermal cells in the root meristem. KOR1 was localized in intracellular compartments corresponding to a heterogeneous population of organelles, which comprised the Golgi apparatus, FM4-64–labeled compartments referred to as early endosomes, and, in the case of GFP-KOR1, the tonoplast. Inhibition of cellulose synthesis by isoxaben promoted a net redistribution of GFP-KOR1 toward a homogeneous population of compartments, distinct from early endosomes, which were concentrated close to the plasma membrane facing the root surface. A redistribution of GFP-KOR1 away from early endosomes was also observed in the same cells at later stages of cell elongation. A subpopulation of GFP-KOR1–containing compartments followed trajectories along the plasma membrane, and this motility required intact microtubules. These observations demonstrate that the deposition of cellulose, like chitin synthesis in yeast, involves the regulated intracellular cycling of at least one enzyme required for its synthesis.

  • Received July 18, 2005.
  • Revised September 14, 2005.
  • Accepted October 13, 2005.
  • Published November 11, 2005.
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An Arabidopsis Endo-1,4-β-d-Glucanase Involved in Cellulose Synthesis Undergoes Regulated Intracellular Cycling
Stéphanie Robert, Adeline Bichet, Olivier Grandjean, Daniel Kierzkowski, Béatrice Satiat-Jeunemaître, Sandra Pelletier, Marie-Theres Hauser, Herman Höfte, Samantha Vernhettes
The Plant Cell Dec 2005, 17 (12) 3378-3389; DOI: 10.1105/tpc.105.036228

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An Arabidopsis Endo-1,4-β-d-Glucanase Involved in Cellulose Synthesis Undergoes Regulated Intracellular Cycling
Stéphanie Robert, Adeline Bichet, Olivier Grandjean, Daniel Kierzkowski, Béatrice Satiat-Jeunemaître, Sandra Pelletier, Marie-Theres Hauser, Herman Höfte, Samantha Vernhettes
The Plant Cell Dec 2005, 17 (12) 3378-3389; DOI: 10.1105/tpc.105.036228
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The Plant Cell Online: 17 (12)
The Plant Cell
Vol. 17, Issue 12
December 2005
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