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The Branching Gene RAMOSUS1 Mediates Interactions among Two Novel Signals and Auxin in Pea

Eloise Foo, Erika Bullier, Magali Goussot, Fabrice Foucher, Catherine Rameau, Christine Anne Beveridge
Eloise Foo
aAustralian Research Council Centre of Excellence for Integrative Legume Research, University of Queensland, St. Lucia, Queensland, 4072, Australia
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Erika Bullier
bStation de Génétique et d'Amélioration des Plantes, Institut J.P. Bourgin, Institut National de la Recherche Agronomique, 78026, Versailles Cedex, France
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Magali Goussot
bStation de Génétique et d'Amélioration des Plantes, Institut J.P. Bourgin, Institut National de la Recherche Agronomique, 78026, Versailles Cedex, France
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Fabrice Foucher
bStation de Génétique et d'Amélioration des Plantes, Institut J.P. Bourgin, Institut National de la Recherche Agronomique, 78026, Versailles Cedex, France
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Catherine Rameau
bStation de Génétique et d'Amélioration des Plantes, Institut J.P. Bourgin, Institut National de la Recherche Agronomique, 78026, Versailles Cedex, France
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Christine Anne Beveridge
aAustralian Research Council Centre of Excellence for Integrative Legume Research, University of Queensland, St. Lucia, Queensland, 4072, Australia
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Published February 2005. DOI: https://doi.org/10.1105/tpc.104.026716

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    Figure 1.

    Working Model of Branching Control in Pea.

    Adapted from Beveridge (2000), Morris et al. (2001), and Beveridge et al. (2003). The model includes a branching inhibitor and a feedback signal that are novel long-distance signals produced in shoot and rootstock. The feedback signal is induced under conditions of low levels or reduced response to the branching inhibitor and upregulates branching inhibitor synthesis and downregulates xylem cytokinin (CK) content. IAA depresses cytokinin both in xylem sap and shoot and increases branching inhibitor levels. Lines with flat ends indicate suppression; arrows represent promotion.

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    Figure 2.

    Structure of the RMS1 Gene, Showing the Relative Positions of Mutations in Various rms1 Alleles.

    Exons are represented by shaded boxes and introns by black lines.

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    Figure 3.

    RMS1 Gene Expression in Different Tissues of Wild-Type Plants.

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    Figure 4.

    RMS1 Gene Expression in Epicotyl of Wild-Type, Single rms Mutant (rms2 to rms5), and Double rms Mutant (rms2 rms3 to rms2 rms5) Plants.

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    Figure 5.

    Branching Phenotype and RMS1 Gene Expression in Reciprocal Grafts between Wild-Type and rms4 Plants 46 d after Grafting.

    (A) Ratio of total lateral length to main stem length (n = 10 to 26).

    (B) RMS1 expression in rootstock epicotyl expressed relative to the wild-type self-grafts.

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    Figure 6.

    RMS1 Expression in Wild-Type Plants over the Course of a Decapitation Experiment.

    RMS1 gene expression at different times in intact plants or plants decapitated in internode 5 treated with 0, 500, or 3000 mg·L−1 IAA to the decapitated stump; internode 5 (A), internode 4 (B), and epicotyl (C). Values are average ± se of two or three pools.

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    Figure 7.

    RMS1 Expression in Internode 4 of Intact or Decapitated Wild-Type, rms2, rms3, and rms4 Plants 12 h after Treatment with 0 or 3000 mg·L−1 IAA.

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    Figure 8.

    RMS1 Expression in Internode 4 of Wild-Type, rms2, rms3, and rms4 Plants 24 h after Intact Plants Were Treated with Ring of 0 or 3000 mg·L−1 of TIBA in Lanolin around Internode 5 or Decapitated in Internode 5.

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    Table 1.

    IAA Level in Wild-Type Plants after Decapitation

    Treatment
    Decapitated
    TimeIntact0500 (mg·L−1 IAA)3000 (mg·L−1 IAA)
    1 h72.0 ± 0.359.8 ± 6.864.5 ± 58.5299 ± 85.7
    3 h76.8 ± 10.845.7 ± 2.2458.7 ± 142.21292 ± 708.0
    • IAA level in internode 5, 1, and 3 h after decapitation and/or replacement of the apex by 0, 500, or 3000 mg·L−1 of IAA. Values are average ± se of two or three pools from the same plants as shown in Figure 6.

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The Branching Gene RAMOSUS1 Mediates Interactions among Two Novel Signals and Auxin in Pea
Eloise Foo, Erika Bullier, Magali Goussot, Fabrice Foucher, Catherine Rameau, Christine Anne Beveridge
The Plant Cell Feb 2005, 17 (2) 464-474; DOI: 10.1105/tpc.104.026716

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The Branching Gene RAMOSUS1 Mediates Interactions among Two Novel Signals and Auxin in Pea
Eloise Foo, Erika Bullier, Magali Goussot, Fabrice Foucher, Catherine Rameau, Christine Anne Beveridge
The Plant Cell Feb 2005, 17 (2) 464-474; DOI: 10.1105/tpc.104.026716
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The Plant Cell Online: 17 (2)
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