Article Figures & Data
Figures
- Figure 1.
Suppression of Oleosins in Arabidopsis.
(A) Scale diagram of the constructs used to suppress OLEO1 using RNAi methods. The Antisense, Hairpin, and Loop cassettes are shown.
(B) Scale diagram of the insertion of a T-DNA element into OLEO1 and OLEO2 genes (lines KnockOLEO1 and KnockOLEO2, respectively). Each gene has two exons (thick line) and one intron (thin line). Both T-DNA insertions are located in the middle of the second exon.
(C) SDS-PAGE profile of oilbody-associated proteins in different plants. The first lane (L) contains the protein ladder (Benchmark; Invitrogen). The second lane contains the oilbody-associated proteins from wild-type (C24) plants. Lanes numbered 1 to 3 contain the oilbody-associated proteins from SupOLEO1-Loop, SupOLEO1-Hairpin, and SupOLEO1-Antisense plants, respectively. Lanes 4 and 5 contain the oilbody-associated proteins from KnockOLEO1 and KnockOLEO2 lines, respectively. The positions of the three most abundant oleosin isoforms are indicated by arrows.
- Figure 2.
Phenotype of Arabidopsis Mature Embryos.
Bright-field microscopy of osmicated embryos thin-sectioned and stained with toluidine blue. Bars = 10 μm.
(A) The wild type. Black arrows indicate oilbodies; arrowheads indicate protein bodies.
(B) SupOLEO1-Loop. White arrows indicate larger oilbodies.
(C) Plant segregated from SupOLEO1-Loop (null).
- Figure 3.
In Vivo Analysis of Oilbody Morphology.
Confocal sections of living mature Arabidopsis embryos stained with Nile red. Large oilbodies in (C), (D), and (F) are shown by arrows. Bars = 2 μm.
(A) Wild-type plant.
(B) SupOLEO1-Antisense plant.
(C) SupOLEO1-Hairpin plant.
(D) SupOLEO1-Loop plant.
(E) KnockOLEO2 plant.
(F) KnockOLEO1 plant.
- Figure 4.
Introduction of a Recombinant Oleosin to Rescue the Oleosin-Deficient Phenotype.
SDS-PAGE profiles of oilbody-associated proteins are shown in left panels and confocal sections of mature embryos in right panels.
(A) SupOLEO1-Loop plant. OLEO1 polypeptide is indicated by the black arrow. White arrows show large oilbodies. Bar = 5 μm.
(B) MaizeOle line. OLEO1 and OLE16 are indicated by the black arrows. Bar = 8 μm.
(C) Progeny from crossing between SupOLEO1-Loop and MaizeOle plants. OLEO1 and OLE16 are indicated by the black arrows. Bar = 5 μm.
- Figure 5.
Comparison of Germination Frequency between Wild-Type and SupOLEO1-Loop Plants in Various Conditions.
Arabidopsis seeds were germinated in different conditions of light and sucrose availability.
(A) Wet filter paper; light.
(B) Wet filter paper; stratified seeds; light.
(C) Half-strength Murashige and Skoog (MS) medium + sucrose; light.
(D) Half-strength MS medium + sucrose; light.
(E) Half-strength MS medium − sucrose; dark.
(F) Half-strength MS medium + sucrose; dark.
- Figure 6.
Fatty Acid Profiles in Wild-Type, Nulls, and Oleosin-Suppressed Seeds of Arabidopsis.
(A) Comparison of major fatty acids (FA) in TAG in wild-type Columbia and oleosin T-DNA insertional knockouts of OLEO1 and OLEO2 in a Columbia background.
(B) Comparison of C24 and segregating nulls from the oleosin-suppressed line SupOLEO1-loop.
- Figure 7.
Fate of Oilbodies during Embryo Development.
Arabidopsis embryos were collected at different stages of development and stained with Nile red. Bars = 40 μm in (A) and (I), 60 μm in (B) and (J), 150 μm in (C) and (K), 20 μm in (D) and (L), and 5 μm in (E) to (H) and (M) to (P).
(A) to (D) Confocal sections of wild-type Arabidopsis embryos at late-heart stage, torpedo stage, walking-stick stage, and mature seed, respectively.
(E) to (H) Same as (A) to (D) in higher magnification.
(I) to (L) Confocal sections of KnockOLEO1 Arabidopsis embryos at late-heart stage, torpedo stage, walking-stick stage, and mature seed, respectively.
(M) to (P) Same as (I) to (L) in higher magnification.
- Figure 8.
Fate of Oilbodies during Seedling Growth.
Arabidopsis seeds were germinated in half-strength MS medium without sucrose supplement. Seedlings with different ages were stained with Nile red and analyzed by confocal microscopy. Nile red was detected in the red channel and autofluorescence of chlorophyll was detected in the blue channel. Bars = 10 μm.
(A) to (H) Confocal sections of wild-type Arabidopsis seedlings at 1 to 8 d after germination, respectively.
(I) to (P) Confocal sections of KnockOLEO1 Arabidopsis seedlings at 1 to 8 d after germination, respectively.
- Figure 9.
Transmission Electron Micrographs of Developing Arabidopsis Embryos.
(A) and (C) Wild-type embryos in the early stages of maturation. Black arrow indicates the ER.
(B) KnockOLEO1 embryo in the early stages of maturation.
(D) and (F) KnockOLEO1 embryo in the middle stages of maturation. Open arrow indicates an elongated oilbody.
(E) Wild-type embryo in the middle stages of maturation.
Bars = 1 μm in (A), (B), and (D) to (F) and 0.1 μm in (C).
- Figure 10.
Model of Oilbody Biogenesis in Oleosin-Suppressed Lines.
The three major components of oilbodies are shown as phospholipids, oleosins (purple), and the TAG matrix (yellow).
(A) Biogenesis of an oilbody in a wild-type cell. The oleosin-saturated environment in ER results in production of lipid bodies completely covered by oleosins.
(B) Biogenesis of an oilbody in an oleosin-suppressed cell. Oilbodies that do not contain enough oleosins to coat their entire surface coalesce, forming larger lipid bodies. These particles might fuse until the surface is completely covered of oleosins.
Tables
Lipid (%) Protein (%) Starch (%) Sucrose (%) Wild type (C24) 40.3 ± 1.4 25.1 ± 1.7 0.5 ± 0.3 3.2 ± 0.4 SupOLEO1-Loop 32.9 ± 2.0 33.9 ± 1.6 0.8 ± 0.4 2.8 ± 0.2 Wild-type (Col-0) 36.1 ± 1.6 35.9 ± 2.4 0.7 ± 0.1 2.9 ± 0.3 KnockOLEO1 30.3 ± 0.9 39.9 ± 1.3 0.8 ± 0.3 2.9 ± 0.1 KnockOLEO2 34.1 ± 1.5 35.8 ± 2.8 0.8 ± 0.4 2.2 ± 0.3 Mean values are given in percentage of seed fresh weight with standard deviations. For lipid, starch, and sucrose analysis, n = 5, and for protein analysis, n = 8.
Supplemental Data
Files in this Data Supplement:
- Supplemental Figure 1 - Comparison of germination frequency between wild-type (Columbia) and KnockOLEO1 plants. Arabidopsis seeds were germinated in wet filter paper in the presence of light.
- Supplemental Figure 2 - Comparison of seedling growth. Arabidopsis seeds were germinated in half strength MS media without sucrose supplement. (A) and (B) Five days old wild-type and SupOLEO1-Loop seedlings, respectively.
- Supplemental Figure 3 - Confocal section of Arabidopsis SupOLEO1 seedlings 8 days after germination. Seeds were germinated in half strength MS media without sucrose supplement, stained with Nile red and analysed by confocal microscopy. Autofluorescence of chlorophyll was detected in the blue channel.
- Supplemental Figure 4 - Suppression of OLEO1 in Arabidopsis using different constructs. Oilbodies were extracted from T2 seeds and submitted to SDS-PAGE. Each lane contains oilbody-associated proteins obtained from individual transgenic events. The events displaying significant reduction of OLEO1 in comparison to OLEO2 and OLEO4 are indicated by arrows. (A), (B) and (C) are transgenic events obtained using Antisense, Hairpin and Loop cassettes, respectively.
- Supplemental Figure 5 - Thin layer chromatography of oilbody lipids. Oilbodies were isolated from different plants and total lipids were extracted from these organelles. Lipids were applied on silica Gel 60 F254 plates and half-developed with chloroform-methanol-acetic acid-formic acid-water (70:30:12:4:2 v/v) and fully developed with hexane-diethyl ether-acetic acid (65:35:2 v/v). The lipids were visualized by heating the plates after they have been dipped in a solution containing cupric acetate (3%) and phosphoric acid (8%). Abbreviations are PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; PI, phosphatidylinositol (PI) and TAG, triacylglycerol.
