Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Research ArticleResearch Article
You have accessRestricted Access

The Downy Mildew Effector Proteins ATR1 and ATR13 Promote Disease Susceptibility in Arabidopsis thaliana

Kee Hoon Sohn, Rita Lei, Adnane Nemri, Jonathan D.G. Jones
Kee Hoon Sohn
Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rita Lei
Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adnane Nemri
Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan D.G. Jones
Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published December 2007. DOI: https://doi.org/10.1105/tpc.107.054262

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • American Society of Plant Biologists

Abstract

The downy mildew (Hyaloperonospora parasitica) effector proteins ATR1 and ATR13 trigger RPP1-Nd/WsB– and RPP13-Nd–dependent resistance, respectively, in Arabidopsis thaliana. To better understand the functions of these effectors during compatible and incompatible interactions of H. parasitica isolates on Arabidopsis accessions, we developed a novel delivery system using Pseudomonas syringae type III secretion via fusions of ATRs to the N terminus of the P. syringae effector protein, AvrRPS4. ATR1 and ATR13 both triggered the hypersensitive response (HR) and resistance to bacterial pathogens in Arabidopsis carrying RPP1-Nd/WsB or RPP13-Nd, respectively, when delivered from P. syringae pv tomato (Pst) DC3000. In addition, multiple alleles of ATR1 and ATR13 confer enhanced virulence to Pst DC3000 on susceptible Arabidopsis accessions. We conclude that ATR1 and ATR13 positively contribute to pathogen virulence inside host cells. Two ATR13 alleles suppressed bacterial PAMP (for Pathogen-Associated Molecular Patterns)-triggered callose deposition in susceptible Arabidopsis when delivered by DC3000 ΔCEL mutants. Furthermore, expression of another allele of ATR13 in plant cells suppressed PAMP-triggered reactive oxygen species production in addition to callose deposition. Intriguingly, although Wassilewskija (Ws-0) is highly susceptible to H. parasitica isolate Emco5, ATR13Emco5 when delivered by Pst DC3000 triggered localized immunity, including HR, on Ws-0. We suggest that an additional H. parasitica Emco5 effector might suppress ATR13-triggered immunity.

INTRODUCTION

Plant innate immunity provides defense against microbial attack and involves at least two components called PAMP (for pathogen-associated molecular pattern)-triggered immunity (PTI) and effector-triggered immunity (ETI) (Chisholm et al., 2006; Jones and Dangl, 2006). The activation of PTI or ETI by direct or indirect recognition of extracellular or intracellular pathogen molecules, respectively, enhances plant disease resistance and restricts pathogen proliferation (Jia et al., 2000; Mackey et al., 2002, 2003; Axtell and Staskawicz, 2003; Deslandes et al., 2003; Zipfel et al., 2004, 2006; Dodds et al., 2006; Ade et al., 2007). However, successful pathogens efficiently suppress plant immunity and cause disease (Grant et al., 2006; Jones and Dangl, 2006).

In Pseudomonas syringae, the type III secretion system (T3SS) has been found to direct the translocation of effectors to the host cell cytoplasm (Jin et al., 2003; Buttner and Bonas, 2006; Cornelis, 2006). The N termini of T3SS effector proteins contain signals required for their translocation (Mudgett and Staskawicz, 1999; Mudgett et al., 2000; Jin et al., 2003). An intact T3SS is required for bacterial virulence and survival in host cells, since T3SS-deficient mutant bacteria cannot grow or cause disease on normally susceptible host plants (Lindgren et al., 1986; Jin et al., 2003). Extensive efforts have been made to uncover in planta functions of T3SS effectors from P. syringae. Several effectors were initially identified as avirulence (Avr) proteins, such as AvrPto and AvrRpt2, which specify race-specific immunity (ETI) upon recognition by tomato (Solanum lycopersicum) Pto and Arabidopsis thaliana RPS2, respectively (Ronald et al., 1992; Innes et al., 1993). In recent years, several P. syringae effectors were shown to suppress Arabidopsis cell wall defense triggered by bacterial PAMPs. AvrPto, AvrE, and HopM1 were found to suppress PAMP-triggered callose deposition in the susceptible Arabidopsis accession Columbia (Col-0) (Hauck et al., 2003; DebRoy et al., 2004). It was further shown that HopM1 recruits At MIN7, a guanine nucleotide exchange factor, for its degradation to suppress PTI via callose deposition (Nomura et al., 2006). In addition, AvrRpm1 and AvrRpt2 were shown to suppress PAMP-triggered callose deposition in Arabidopsis plants lacking corresponding R genes (RPM1 and RPS2, respectively) (Kim et al., 2005). Recently, HopAI1 was shown to be a phosphothreonine lyase that inactivates mitogen-activated protein kinases, disabling the oxidative burst and callose deposition (Zhang et al., 2007). In addition, P. syringae pv tomato (Pst) DC3000 HopU1 was found to ADP-ribosylate a RNA binding protein, GRP7, to suppress callose deposition and interfere with host defense, thus enhancing pathogen virulence (Fu et al., 2007).

In oomycete pathogens, such as Phytophthora spp and Hyaloperonospora parasitica, genes homologous with T3SS protein secretion components are absent. However, recent identification of several oomycete pathogen effectors that are presumably translocated to the host cell cytoplasm suggests that oomycete effectors may use an evolutionarily conserved mechanism to deliver effector proteins into plant cells (Allen et al., 2004; Shan et al., 2004; Armstrong et al., 2005; Rehmany et al., 2005). Four effector proteins from oomycete pathogens, ATR1 and ATR13 (H. parasitica), Avr3a (P. infestans), and Avr1b (P. sojae), contain an N-terminal signal peptide and an RxLR motif (Allen et al., 2004; Shan et al., 2004; Armstrong et al., 2005; Rehmany et al., 2005). The RxLR motif is similar to the RxLx motif that is required for the translocation of proteins from malaria parasites (Plasmodium spp) to host cells (Hiller et al., 2004; Haldar et al., 2006; Kamoun, 2006). An oomycete RxLR motif can confer host cell targeting in Plasmodium (Bhattacharjee et al., 2006). However, the mechanism of RxLR-mediated translocation of oomycete (and Plasmodium) effector proteins into host cells is still unknown.

Three tightly linked Arabidopsis R genes, RPP1 (for Recognition of Peronospora parasitica)-WsA, RPP1-WsB, and RPP1-WsC, were cloned from accession Wassilewskija (Ws-0) (Botella et al., 1998). Each of these RPP1 genes specifies resistance to different H. parasitica isolates. In Arabidopsis accession Niederzenz (Nd), resistance is specified by a locus containing RPP1-Nd. The corresponding Avr gene, designated ATR1NdWsB (for Arabidopsis thaliana Recognized1), recognized by RPP1-Nd was isolated by map-based cloning using a population derived from a cross between H. parasitica isolates Emoy2 and Maks9, which are avirulent and virulent, respectively, on Nd-0 (Rehmany et al., 2003, 2005). The H. parasitica Emoy2 allele of ATR1NdWsB is recognized by both RPP1-Nd and RPP1-WsB, whereas the Maks9 allele is recognized only by RPP1-WsB (Rehmany et al., 2005). In addition, the H. parasitica Cala2 allele of ATR1NdWsB is not recognized by either RPP1-WsB or RPP1-Nd (Rehmany et al., 2005). Arabidopsis accessions Col-0 and Nd-0 were found to be susceptible and resistant, respectively, to H. parasitica isolate Maks9, and resistance is conferred by RPP13-Nd (Bittner-Eddy et al., 1999). Col-5 (a glabrous derivative of Col-0) plants transformed with a cosmid clone containing RPP13-Nd are fully resistant to H. parasitica Maks9 (Bittner-Eddy et al., 2000). Subsequently, ATR13 alleles were cloned from several isolates of H. parasitica (Allen et al., 2004). Using biolistic bombardment of Col-5 and Col-5 (RPP13-Nd), Allen et al. (2004) characterized several ATR13 alleles. In addition, amino acid sequence alignments of avirulent and virulent alleles of ATR13 suggested that ATR13 has undergone diversifying selection, consistent with the polymorphism of the RPP13 locus in different Arabidopsis accessions (Allen et al., 2004; Rose et al., 2004). However, the functions of these oomycete effectors during pathogenesis in susceptible host plants are unknown. Analysis of the functions of oomycete effectors will help reveal how oomycete pathogens overcome host recognition and promote plant disease susceptibility.

The focus of our current research has been to test the hypothesis that ATR1 and ATR13 are virulence determinants during H. parasitica pathogenesis in Arabidopsis. A bacterial T3SS-based effector delivery system was developed to overcome the difficulties of studying effector functions in H. parasitica. We show that chimeric proteins of bacterial (AvrRPS4) and oomycete (ATR1 and ATR13) effectors can be efficiently delivered to plant cells by Pst DC3000 and trigger allele-specific ETI in resistant Arabidopsis plants carrying the corresponding RPP gene. We also demonstrate that several alleles of ATR1 and ATR13 can positively contribute to pathogen virulence in susceptible Arabidopsis accession Col-0 when delivered by Pst DC3000, suggesting that these effectors may interfere with host mechanisms involved in resistance to both bacterial and oomycete pathogens. Furthermore, ATR13 suppresses PAMP-triggered callose deposition at Arabidopsis leaf cell walls and reactive oxygen species (ROS) production, also consistent with the idea that ATR13 suppresses host cell immunity. However, ATR13 cannot fully restore the virulence of Pst DC3000 lacking a known bacterial suppressor of callose deposition, HopM1, indicating that ATR13 may suppress oomycete PTI by a mechanism that is distinct from HopM1. Finally, we show that even though H. parasitica Emco5 is virulent on Ws-0, ATR13Emco5 triggers race-specific immunity in Ws-0, and we propose that ATR13Emco5 recognition might be suppressed by an unknown effector(s) of H. parasitica Emco5.

RESULTS

AvrRPS4 Amino Acids 1 to 136 Can Direct AvrRpt2 Effector Protein into Plant Cells via the Pst DC3000 Type III Secretion Machinery

AvrRPS4 protein is secreted by the Pst DC3000 T3SS and is subsequently processed to a smaller form in plant cells (K.H. Sohn, Y. Zhang, and J.D.G. Jones, unpublished data). The in planta processing site of AvrRPS4 was identified as Gly-133–Gly-134 in Nicotiana benthamiana and Arabidopsis (K.H. Sohn, Y. Zhang, and J.D.G. Jones, unpublished data). Furthermore, it was shown that the C-terminal 88 amino acids of AvrRPS4 are sufficient for avirulence function. These findings led us to test whether AvrRPS4 N-terminal fusions to other proteins might be delivered into and processed in plant cells. A broad host range vector, pBBR 1MCS-5, carrying the nucleotide sequences encoding the N-terminal 136 amino acids of AvrRPS4 regulated by the AvrRPS4 promoter, was constructed and named pEDV3 (for Effector Detector Vector 3) (Figure 1A ). Pst DC3000 carrying pBBR 1MCS-5 or pEDV3 grew to a similar level compared with the wild-type strain carrying no broad host range vector (see Supplemental Figure 1 online). To test the AvrRPS4-mediated delivery of effector proteins into plant cells and the in planta processing of AvrRPS4 fusion proteins using this vector, we used it to deliver amino acids 72 to 255 of AvrRpt2, which are required for its effector function (Mudgett and Staskawicz, 1999). First, we tested whether Pst DC3000 expressing AvrRPS41-136-AvrRpt272-255-HA (hence, AvrRPS4N-AvrRpt2-HA) can trigger RPS2-dependent localized immune responses in Arabidopsis. Pst DC3000 carrying pEDV3 (AvrRPS4N-AvrRpt2-HA) triggered a hrp-dependent hypersensitive response (HR) in accession Col-0, which carries functional RPS2, but not in rps2-101c (Figure 1B). Furthermore, when inoculated at low concentrations, Pst DC3000 carrying pEDV3 (AvrRPS4N-AvrRpt2-HA) caused disease on rps2-101c plants but not on wild-type plants (Figure 1C). In addition, Pst DC3000 carrying pEDV3 (AvrRPS4N-AvrRpt2-HA) showed significantly reduced growth in Col-0 (RPS2), whereas this strain grew comparably to Pst DC3000 carrying pEDV3 (AvrRPS4N-HA) in rps2-101c (see Supplemental Figure 2 online).

Figure 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1.

AvrRPS4 Can Direct the AvrRpt2 Effector Protein into Plant Cells via the Pst DC3000 Type 3 Secretion Machinery.

(A) Construction of pEDV3. The effectors AvrRpt2, ATR1, and ATR13 were cloned in-frame with the N-terminal 136 amino acids encoding T3S signal to express AvrRPS4-Effector-HA chimeric proteins in Pst DC3000. The expression of the chimeric effector proteins were designed to be driven by 128 bp of native AvrRPS4 promoter.

(B) AvrRPS41-136, designated AvrRPS4N, delivers AvrRpt272-255-HA by the Pst DC3000 T3SS and triggers RPS2-dependent HR in Arabidopsis accession Col-0. Leaves of wild-type Col-0 and rps2-101c were hand-inoculated using a 1-mL syringe with 5 × 107 colony-forming units (cfu)/mL Pst DC3000 wild type or ΔhrcC mutant carrying pEDV3 (AvrRPS4N) or pEDV3(AvrRPS4N-AvrRpt272-255). Labeling refers to the amino acid number in the respective protein. The red arrow indicates the leaf showing AvrRpt2-triggered HR. Photographs were taken at 22 h after infection. This experiment was repeated six times with similar results.

(C) Pst DC3000 expressing AvrRPS4N-AvrRpt2 is unable to cause disease symptoms on Col-0 (RPS2). Leaves of wild-type Col-0 and rps2-101c were hand-inoculated using a 1-mL syringe with 5 × 105 cfu/mL Pst DC3000 wild type or ΔhrcC mutant carrying pEDV3 (AvrRPS4N) or pEDV3(AvrRPS4N-AvrRpt2). The red arrow indicates the leaf showing no disease symptoms caused by AvrRpt2-triggered immunity. Photographs were taken at 5 d after inoculation. This experiment was repeated six times with similar results.

(D) AvrRPS4N-ATR13Emco5 is processed in planta. Wild-type Col-0 leaves were hand-inoculated using a 1-mL syringe with 2 × 109 cfu/mL Pst DC3000 wild type expressing Embedded Image. Leaf samples were taken at 0, 5, and 10 h after infection (hpi) for protein extraction and immunoblot analysis using anti-HA antibody. The top bands show unprocessed AvrRPS4N-ATR13Emco5 protein, and the bottom band represents processed ATR13Emco5. This experiment was repeated twice with similar results.

AvrRPS41-136 Can Deliver Oomycete Effector ATR13 to Arabidopsis Leaf Cells

ATR13 is an H. parasitica effector protein that is recognized by a coiled coil–nucleotide binding–leucine-rich repeat protein, RPP13, in Arabidopsis accession Nd-0 (Allen et al., 2004). ATR13Emoy2 and ATR13Emco5 are virulent and avirulent alleles, respectively, on RPP13-Nd. The N-terminal signal peptide and RxLR motif of oomycete effectors are thought to be required for their translocation from haustorium to plant cell during pathogenesis (Bhattacharjee et al., 2006; Birch et al., 2006). Since the signal peptide and RxLR motif of ATR13 are not necessary for its avirulence function (Allen et al., 2004), we engineered Embedded Image and Embedded Image (hence, AvrRPS4N-ATR13Emoy2 and AvrRPS4N-ATR13Emco5) fusion protein constructs without the signal peptide and RxLR motif of ATR13.

When Pst DC3000 pEDV3 (AvrRPS4N) and pEDV3 (AvrRPS4N-ATR13Emco5) were grown in hrp-inducing minimal medium, the fusion proteins were detected as full-length protein without processing (see Supplemental Figure 3 online). However, we could detect processed ATR13Emco5-HA protein from the protein extracts of leaves infiltrated with Pst DC3000 expressing AvrRPS4N-ATR13Emco5 on Arabidopsis Col-0 leaves using anti-HA antibody (Figure 1D). We detected hrp-dependent expression of full-length AvrRPS4N-ATR13Emco5 at 5 h after infection and processed ATR13Emco5-HA protein at 10 h after infection (Figure 1D). These results demonstrated that AvrRPS4N can deliver C-terminally tagged effector proteins to plant cells, where they are subsequently processed. Therefore, we concluded that the AvrRPS4N-mediated effector delivery system can deliver oomycete effector proteins into plant cells.

An Avirulence Allele of ATR13 Triggers RPP13-Nd–Dependent HR in Arabidopsis When Delivered by Pst DC3000

We next tested RPP13-Nd–dependent HR elicitation of Arabidopsis plants in response to Pst DC3000 expressing AvrRPS4N, AvrRPS4N-ATR13Emoy2, or AvrRPS4N-ATR13Emco5. Arabidopsis Col-0, which carries an RPP13 allele that recognizes neither ATR13 allele, did not manifest HR after infiltration of any of the strains (Figure 2 ). Pst DC3000 expressing AvrRPS4N-ATR13Emco5 triggered strong HR in Col-5 plants carrying RPP13-Nd and wild-type Nd-0 plants, whereas AvrRPS4N and AvrRPS4N-ATR13Emoy2 did not elicit HR symptoms at 24 h after infection in Col-5 (RPP13-Nd) and Nd-0. As expected, HR triggered by AvrRPS4N-ATR13Emco5 required Pst DC3000 T3SS (Figure 2). These results indicate that T3SS-dependent expression and translocation of AvrRPS4N can deliver C-terminally tagged effector domains of ATR13 alleles to plant cells, resulting in allele-specific and RPP13-Nd–dependent HR.

Figure 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 2.

ATR13Emco5 Triggers RPP13-Nd–Dependent HR When Delivered by Pst DC3000.

Col-0, Col-5 (RPP13-Nd), and Nd-0 leaves were hand-inoculated using a 1-mL syringe with 5 × 107 cfu/mL Pst DC3000 wild type or ΔhrcC mutant carrying pEDV3 (AvrRPS4N), pEDV3 (Embedded Image), or pEDV3 (Embedded Image). Labeling refers to the allele or amino acid number in the respective protein. Red arrows indicate the leaves showing ATR13Emco5-triggered HR. Photographs were taken at 22 h after infection. The experiment was repeated five times with similar results.

ATR13Emco5 Restricts the Growth of Pst DC3000 on Arabidopsis Lines Carrying RPP13-Nd

We next tested whether HR elicited in Col-5 (RPP13-Nd) and Nd-0 by ATR13Emco5-HA is correlated with restricted growth and reduced disease symptoms after infiltration with low bacterial inocula. Wild-type Col-0 leaves developed chlorotic and necrotic lesions at 4 to 5 d after inoculation of Pst DC3000 expressing AvrRPS4N, AvrRPS4N-ATR13Emoy2, or AvrRPS4N-ATR13Emco5 (Figure 3A ). Col-5 (RPP13-Nd) and Nd-0 also showed typical disease symptoms caused by Pst DC3000 after inoculation with Pst DC3000 expressing AvrRPS4N or AvrRPS4N-ATR13Emoy2. However, Col-5 (RPP13-Nd) and Nd-0 did not show disease symptoms when infected by Pst DC3000 expressing AvrRPS4N-ATR13Emco5, suggesting that attenuation of disease symptoms was due to the activation of RPP13-Nd–dependent resistance upon recognition of ATR13Emco5. We measured the growth of Pst DC3000 expressing AvrRPS4N, AvrRPS4N-ATR13Emoy2, or AvrRPS4N-ATR13Emco5 in infected Col-0, Col-5 (RPP13-Nd), and Nd-0 plants. Col-0, Col-5 (RPP13-Nd), and Nd-0 plants allowed Pst DC3000 expressing AvrRPS4N to grow to high levels (Figure 3B). However, the growth of Pst DC3000 expressing AvrRPS4N-ATR13Emco5 was significantly reduced in Col-5 (RPP13-Nd) and Nd-0 plants, indicating that the recognition of ATR13Emco5 by RPP13-Nd in Arabidopsis caused restricted in planta bacterial growth when delivered from Pst DC3000.

Figure 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 3.

ATR13Emoy2 and ATR13Emco5 Confer Enhanced Virulence of Pst DC3000 in Arabidopsis.

(A) Disease symptoms of Arabidopsis leaves caused by Pst DC3000. Col-0, Col-5 (RPP13-Nd), and Nd-0 leaves were hand-inoculated using a 1-mL syringe with 5 × 105 cfu/mL Pst DC3000 wild type carrying pEDV3 (AvrRPS4N), pEDV3 (AvrRPS4N-ATR13Emoy2), or pEDV3 (AvrRPS4N-ATR13Emco5). Red arrows indicate the leaves showing no symptoms due to ATR13Emco5-triggered immunity. Photographs were taken at 5 d after inoculation. This experiment was repeated four times with similar results.

(B) Leaf bacterial populations of Arabidopsis plants infected with Pst DC3000. Bacterial infection was done as described for (A). Bacterial populations were measured at 0 and 4 d after inoculation (dpi). Each bar represents the mean number of bacterial colonies recovered on selective agar medium containing appropriate antibiotics from four independent replicates. This experiment was repeated three times with similar results. Single (P < 0.05) and double (P < 0.01) asterisks represent significant differences compared with Pst DC3000 (AvrRPS4N-HA).

Two Alleles of ATR13 Confer Enhanced Virulence of Pst DC3000 on Susceptible Arabidopsis Genotypes

ATR13 triggers localized immunity resulting in enhanced resistance to Pst DC3000 in Arabidopsis plants carrying RPP13-Nd. We also observed that Pst DC3000 expressing AvrRPS4N-ATR13Emoy2 or AvrRPS4N-ATR13Emco5 conferred enhanced growth compared with Pst DC3000 expressing AvrRPS4N in susceptible Arabidopsis (Col-0) plants (Figures 3A and 3B). The elevated growth of Pst DC3000 resulted in enhanced disease symptoms in Col-0 plants infected with Pst DC3000 expressing either AvrRPS4N-ATR13Emoy2 or AvrRPS4N-ATR13Emco5 (Figure 3A). In addition, AvrRPS4N-ATR13Emoy2 conferred enhanced growth of Pst DC3000 in Col-5 (RPP13-Nd), indicating that the virulence function of the ATR13Emoy2 allele is independent of RPP13-Nd. However, ATR13Emoy2 did not confer significantly enhanced growth of Pst DC3000 on Nd-0 at 3 d after inoculation. We obtained similar results by spraying bacteria on Arabidopsis leaves. Overall, these data indicate that the delivery of ATR13 alleles with distinct specificities by bacterial T3SS can confer enhanced virulence to Pst DC3000 growing on Arabidopsis.

ATR1Emoy2 Triggers RPP1-Nd– and RPP1-WsB–Dependent HR in Arabidopsis and Confers Resistance When Delivered by Pst DC3000

To examine further whether the pEDV system can deliver other H. parasitica effectors, we tested ATR1 (Rehmany et al., 2005). Like ATR13, ATR1 is highly polymorphic and coevolves with its host RPP locus. While ATR1Emoy2 is recognized by both RPP1-Nd and RPP1-WsB, ATR1Maks9 is recognized by RPP1-WsB but not RPP1-Nd, and ATR1Cala2 is not recognized by RPP1-WsB or RPP1-Nd (Rehmany et al., 2005). We fused AvrRPS4N to the Emoy2 and Cala2 alleles of ATR1, starting at the RxLR motif, to construct Embedded Image and Embedded Image (hence, AvrRPS4N-ATR1Emoy2 and AvrRPS4N-ATR1Cala2), respectively. Arabidopsis Ws-0 and Nd-0, which carry RPP1-WsB and RPP1-Nd, showed HR in response to Pst DC3000 expressing AvrRPS4N-ATR1Emoy2 but not AvrRPS4N-ATR1Cala2 or the empty vector control (Figure 4 ). As expected, this allele-specific HR was not observed in Col-0, which contains neither RPP1-WsB nor RPP1-Nd. The same constructs for both alleles of ATR1 lacking the epitope HA tag were made and gave the same results (see Supplemental Figure 4 online).

Figure 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 4.

ATR1Emoy2 Triggers RPP1-Dependent HR When Delivered by Pst DC3000.

Col-0, Col-5 (RPP13-Nd), and Nd-0 leaves were hand-inoculated using a 1-mL syringe with 5 × 107 cfu/mL Pst DC3000 wild type or ΔhrcC mutant carrying pEDV3 (AvrRPS4N), pEDV3(Embedded Image), or pEDV3 (Embedded Image). Labeling refers to the allele or amino acid number in the respective protein. Red arrows indicate the leaves showing ATR1Emoy2-triggered HR. Photographs were taken at 22 h after infection.

ATR1 Enhances the Virulence of Pst DC3000 on Arabidopsis

We examined disease progression caused by Pst DC3000 expressing either AvrRPS4N-ATR1Emoy2 or AvrRPS4N-ATR1Cala2 in Col-0, Ws-0, and Nd-0. As expected, Pst DC3000 carrying pEDV3 (AvrRPS4N-ATR1Emoy2) did not cause any chlorotic disease symptoms in Ws-0 or Nd-0, which correlated with the restricted growth of the bacteria (Figures 5A and 5B ). However, Pst DC3000 carrying pEDV3 (AvrRPS4N-ATR1Cala2) conferred enhanced disease symptoms on Col-0 and Ws-0 but not on Nd-0 (Figure 5A). The enhanced symptoms caused by ATR1Cala2 were correlated with elevated bacterial growth in the absence of any recognition event (Figure 5B), suggesting a contribution of ATR1Cala2 to virulence when delivered by Pst DC3000. In addition, ATR1Emoy2 also enhanced bacterial growth compared with Pst DC3000 carrying pEDV3 (AvrRPS4N) in the susceptible Arabidopsis accession Col-0, consistent with a virulence function of ATR1Emoy2 in the absence of RPP1 (Figure 5B).

Figure 5.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 5.

ATR1Emoy2 and ATR1Cala2 Confer Enhanced Virulence of Pst DC3000 in Arabidopsis.

(A) Disease symptoms of Arabidopsis leaves caused by Pst DC3000. Col-0, Col-5 (RPP13-Nd), and Nd-0 leaves were hand-inoculated using a 1-mL syringe with 5 × 105 cfu/mL Pst DC3000 wild type carrying pEDV3 (AvrRPS4N), pEDV3 (AvrRPS4N-ATR1Emoy2), or pEDV3 (AvrRPS4N-ATR1Cala2). Red arrows indicate the leaves showing no symptoms due to ATR1Emoy2-triggered immunity. Photographs were taken at 5 d after inoculation.

(B) Leaf bacterial populations of Arabidopsis plants infected with Pst DC3000. Bacterial infection was done as described for (A). Bacterial populations were measured at 0 and 3 d after inoculation (dpi). Each bar represents the mean number of bacterial colonies recovered on selective agar medium containing appropriate antibiotics from four independent replicates. Single (P < 0.05) and double (P < 0.01) asterisks represent significant differences compared with Pst DC3000 (AvrRPS4N-HA).

Two Alleles of ATR13 Suppress PAMP-Triggered Callose Deposition and Enhance Bacterial Virulence

ATR13 confers enhanced virulence on Pst DC3000 growing on Arabidopsis (Figure 3). We investigated how ATR13 confers enhanced virulence in susceptible Arabidopsis accessions. Since H. parasitica makes intimate contact with host cell walls during pathogenesis, we hypothesized that one important function of H. parasitica effectors might be the suppression of cell wall–based defense. Recently, three Pst DC3000 effectors, AvrPto, AvrE, and HopM1, were shown to suppress PAMP-triggered cell wall defense (callose deposition) when expressed in planta or delivered to Arabidopsis Col-0 leaf cells (Hauck et al., 2003; DebRoy et al., 2004; Nomura et al., 2006). HopM1 is located on conserved effector locus (CEL), and the Pst DC3000 ΔCEL strain can no longer suppress callose deposition (Alfano et al., 2000; DebRoy et al., 2004). Callose suppression of Pst DC3000 ΔCEL can be restored by introducing a plasmid (pORF43) expressing HopM1 and its chaperone, SchM (DebRoy et al., 2004). Therefore, we examined whether ATR13 can functionally complement AvrPto- or HopM1-mediated suppression of callose deposition using Pst DC3000 ΔAvrPto or in a ΔCEL mutant strain.

Col-0 exhibited extensive callose deposition when inoculated with the Pst DC3000 ΔhrcC mutant, whereas essentially no callose deposition was observed in leaves infiltrated with the wild-type Pst DC3000 (Figure 6A ) (Hauck et al., 2003). Infiltration of water did not induce any callose deposition. As expected, Pst DC3000 ΔCEL wild type or carrying pEDV3 (AvrRPS4N) was completely unable to suppress callose deposition in Col-0, whereas Pst DC3000 ΔCEL carrying pORF43 (HopM1/ShcM) could restore the callose suppression phenotype to the level of wild-type Pst DC3000 (Figure 6A) (DebRoy et al., 2004). We tested whether two alleles of ATR13 could suppress callose deposition induced by Pst DC3000 ΔCEL. Significantly, Pst DC3000 ΔCEL carrying pEDV3 (AvrRPS4N-ATR13Emoy2) or pEDV3 (AvrRPS4N-ATR13Emco5) suppressed callose deposition induced by Pst DC3000 ΔCEL to a level comparable to that of Pst DC3000 ΔCEL carrying pORF43 (HopM1/ShcM) (Figure 6A). We were unable to observe the accumulation of callose after inoculation of Pst DC3000 ΔAvrPto or ΔAvrPto/AvrPtoB in Col-0; therefore, we did not investigate ATR13-triggered callose suppression further with these mutants.

Figure 6.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 6.

ATR13 Suppresses PAMP-Triggered Callose Deposition at Susceptible Arabidopsis Leaf Cell Walls.

(A) Five-week-old Col-0 leaves were hand-inoculated with sterilized water or 1 × 108 cfu/mL suspensions of Pst DC3000 ΔhrcC mutant or wild type, Pst DC3000 ΔCEL mutant, or Pst DC3000 ΔCEL mutant carrying pORF43 (HopM1/ShcM), pEDV3 (AvrRPS4N), or pEDV3 (AvrRPS4N-ATR13Emco5). Leaf samples were taken at 14 h after infection and stained with aniline blue for visualization of callose (white dots). This experiment was repeated four times with similar results.

(B) Leaf bacterial populations of 5-week-old Arabidopsis Col-0 leaves infected with Pst DC3000 wild type or ΔCEL strains. Five-week-old Arabidopsis Col-0 leaves were hand-inoculated using a 1-mL syringe with 5 × 105 cfu/mL Pst DC3000 wild type or Pst DC3000 ΔCEL carrying pEDV3 (AvrRPS4N), pORF43 (HopM1/ShcM), pEDV3 (AvrRPS4N-ATR13Emoy2), or pEDV3 (Embedded Image). This experiment was repeated three times with similar results. Asterisks (P < 0.01) represents significant differences compared with Pst DC3000 ΔCEL (AvrRPS4N-HA). dpi, d after inoculation.

We tested whether the two alleles of ATR13 can also enhance Pst DC3000 ΔCEL growth on susceptible host plants. Pst DC3000 ΔCEL carrying pEDV3(AvrRPS4N-HA) grew ∼100-fold less than Pst DC3000 wild type, and the decreased growth of the Pst DC3000 ΔCEL mutant was recovered to a level comparable to that of wild-type Pst DC3000 by delivering HopM1/SchM (pORF43) (Figure 6B). Pst DC3000 ΔCEL carrying pEDV3 (AvrRPS4N-ATR13Emoy2-HA) or pEDV3 (AvrRPS4N-ATR13Emco5-HA) grew ∼10-fold more than Pst DC3000 ΔCEL carrying pEDV3(AvrRPS4N-HA), suggesting that both alleles of ATR13 can enhance pathogen virulence (Figure 6B).

Transient Expression of ATR13 in Arabidopsis Leaf Cells Suppresses Two PTI Phenotypes

We tested whether ATR13 can suppress flg22-triggered defense responses in transgenic Arabidopsis (Col-5) plants conditionally expressing ATR13Maks9 from a dexamethasone-inducible promoter (Allen et al., 2004). Spraying 5-week-old Col-5 (Dex-ATR13Maks9) plants with dexamethasone solution induced the transcription of ATR13Maks9 (Figure 7C ). Infiltration of flg22 triggered substantial callose deposition in wild-type Col-0 leaves with or without pretreatment of dexamethasone (Figure 7A). In Col-5 (Dex-ATR13Maks9), flg22-triggered callose depositions were greatly suppressed when the leaves were sprayed with dexamethasone before flg22 treatment, confirming that ATR13 suppresses PAMP-triggered callose depositions in plant cell (Figure 7A). It has been shown that flg22 triggers the production of ROS (Felix et al., 1999). Therefore, we tested whether the expression of ATR13 suppresses flg22-triggered ROS production. Dexamethasone-induced expression of ATR13Maks9 suppressed the flg22-triggered ROS burst by approximately threefold, suggesting that ATR13 might act upstream of ROS production in the PTI signaling pathway (Figure 7B).

Figure 7.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 7.

Expression of ATR13 in Plant Cells Suppresses PAMP-Triggered Immune Responses.

(A) Five-week-old Arabidopsis Col-0 and Col-5 (Dex-ATR13Maks9) plants were sprayed with water or 20 μM dexamethasone (Dex; 0.02% Silwet-77). Twenty-four hours later, the leaves were infiltrated with water or 1 μM flg22. Leaf samples were taken at 8 h after flg22 infiltration and stained with aniline blue for visualization of callose (white dots). This experiment was repeated twice with similar results.

(B) Leaf discs from 5-week-old Col-5 (Dex-ATR13Maks9) plants were taken and floated on water or 20 μM dexamethasone solution prior to flg22 treatment. Luminescence was measured every 2 min for at least 60 min after treatment with flg22 using the Photek camera system. Lines and error bars represent means and se, respectively, of 48 independent samples tested at the same time. The same experiments were done twice with similar results.

(C) Expression analysis of ATR13Maks9 in Col-5 (Dex-ATR13Maks9) plants. PCR products were amplified from cDNA derived from Col-5 (Dex-ATR13Maks9) leaves sprayed with water or 20 μM dexamethasone (0.02% Silwet-77). PCR was performed using gene-specific primers and separated on a 1.2% agarose gel. At Actin (At5g09810) was also amplified from the same cDNA samples as a control.

H. parasitica Race Emco5 Is Virulent on Ws-0, yet ATR13Emco5 Triggers HR and Restricts Bacterial Growth When Delivered from Pst DC3000

H. parasitica Emco5 is resisted in Arabidopsis plants carrying RPP13-Nd (Bittner-Eddy et al., 2000) but will infect accession Col-0 (Figure 8A ). Consistent with this, Nd-0 showed HR symptoms when infiltrated with a 5 × 107 cfu/mL suspension of Pst DC3000 carrying pEDV3 (AvrRPS4N-ATR13Emco5), whereas Col-0 did not (Figures 2 and 8A). Ws-0 was also highly susceptible to H. parasitica Emco5 (Figure 8A) (Botella et al., 1998). However, Ws-0 showed HR when inoculated with a 5 × 107 cfu/mL suspension of Pst DC3000 carrying pEDV3 (AvrRPS4N-ATR13Emco5), whereas Pst DC3000 carrying pEDV3 (AvrRPS4N) did not trigger any visible symptoms. We examined whether Ws-0 exhibits restricted bacterial growth when ATR13Emco5 is delivered by Pst DC3000. In Ws-0, bacterial growth was reduced by at least 10-fold at 3 d after inoculation of Pst DC3000 carrying pEDV3 (AvrRPS4N-ATR13Emco5) compared with pEDV3 (AvrRPS4N) (Figure 8C). Transcriptional expression of ATR13Emco5 was confirmed in Col-0, Nd-0, and Ws-0 plants infected with H. parasitica Emco5 using RT-PCR with gene-specific primers (Figure 8B). The nucleotide sequence of the amplified ATR13Emco5 cDNA fragment was confirmed by sequencing after inoculation of Ws-0 with Emco5.

Figure 8.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 8.

ATR13Emco5-Triggered Immunity Is Suppressed by H. parasitica Isolate Emco5 in Ws-0.

(A) Arabidopsis accessions Col-0, Nd-0, and Ws-0 leaves were sprayed with 105 spores/mL H. parasitica isolate Emco5 (top panel; 2-week-old leaves) or hand-inoculated with 5 × 107 cfu/mL suspensions of Pst DC3000 carrying pEDV3 (AvrRPS4N) or pEDV3 (AvrRPS4N-ATR13Emco5) (middle and bottom panels, respectively; 6-week-old leaves). Photographs were taken at 10 d after inoculation or 22 h after infection for H. parasitica Emco5– or Pst DC3000–infected leaves, respectively. Red arrows indicate ATR13Emco5-triggered HR. This experiment was repeated three times with similar results.

(B) Expression analysis of ATR13Emco5 in infected Arabidopsis seedlings. PCR products were amplified from cDNA derived from H. parasitica Emco5–infected 2-week-old Col-0, Nd-0, or Ws-0 seedlings using gene-specific primers and separated on a 1.2% agarose gel. H. parasitica methylenetetrahydrofolate dehydrogenase (MTD) was also amplified from the same cDNA samples as a control.

(C) Bacterial populations of Ws-0 leaves infected with Pst DC3000. Leaves were hand-inoculated using a 1-mL syringe with 5 × 105 cfu/mL wild-type Pst DC3000 carrying pEDV3 (AvrRPS4N), pEDV3 (AvrRPS4N-ATR13Emoy2), or pEDV3 (AvrRPS4N-ATR13Emco5). Bacterial populations were measured at 0 and 3 d after inoculation (dpi). Each bar represents the mean number of bacterial colonies recovered on selective agar medium containing appropriate antibiotics from four independent replicates. This experiment was repeated twice with similar results. The asterisk (P < 0.01) represents a significant difference compared with Pst DC3000 (AvrRPS4N-HA).

DISCUSSION

We demonstrate here that two effectors, ATR1 and ATR13, from the filamentous oomycete pathogen H. parasitica suppress host PTI during a compatible interaction and trigger ETI in resistant Arabidopsis accessions when delivered into Arabidopsis leaf cells by the Pst DC3000 T3SS. Furthermore, three alleles of ATR13 suppress PAMP-triggered callose deposition at Arabidopsis leaf cell walls. We also show that ATR13Maks9 can suppress the PAMP-triggered ROS burst on susceptible host plants. Additionally, we suggest that H. parasitica isolate Emco5 might carry additional effector(s) that suppress ATR13Emco5-triggered immunity in Arabidopsis accession Ws-0.

Implications of T3SS-Mediated Delivery of Nonbacterial Proteins

Extensive efforts have been made to identify effector proteins from bacterial, fungal, and oomycete plant pathogens. There are several well-known strategies that have been used to screen bacterial effector proteins that are secreted to plant cells. For example, truncated forms of avirulence proteins, such as AvrRpt2 or AvrBs2, lacking domains required for T3SS have been introduced to bacterial chromosomes by engineered transposons to identify T3S effectors enabling secretion of the reporter proteins (Guttman et al., 2002; Roden et al., 2004). Moreover, bioinformatic analysis has been performed to predict T3S effectors from sequenced plant pathogenic bacteria (Petnicki-Ocwieja et al., 2002; Vinatzer et al., 2006).

Since bacterial effectors are likely to be secreted by T3SS in an unfolded state, it is remarkable that AvrRPS4-ATR chimeric proteins were delivered by P. syringae T3SS and elicited defense responses in host plants (Stebbins and Galan, 2001; Akeda and Galan, 2005). In some cases, secretion of T3S effectors is dependent on interaction with their chaperones, and often the chaperone binding domain resides immediately after the N-terminal effector secretion signal (Fu and Galan, 1998; Lee and Galan, 2004). Recent evidence suggests that unfolding of T3S effector proteins by an ATPase is required for secretion (Akeda and Galan, 2005). It is possible that 1 to 136 amino acids of AvrRPS4 carries a chaperone binding or ATPase-interacting domain that is required for efficient unfolding and secretion.

During interactions of filamentous oomycete pathogens (such as P. infestans and H. parasitica) with host plants, a set of effectors are thought to be translocated to plant cells (Birch et al., 2006; Kamoun, 2006). However, it is not clear how these pathogen molecules are delivered. P. infestans and H. parasitica effectors usually carry a signal peptide and an RxLR amino acid motif (Allen et al., 2004; Shan et al., 2004; Armstrong et al., 2005; Rehmany et al., 2005; Kamoun, 2006). The RxLR motif of a P. infestans effector, Avr3a, can functionally complement the RxLx motif that is required for the translocation of Plasmodium falciparum effector proteins into hosts (Hiller et al., 2004; Bhattacharjee et al., 2006). Recently, it was shown that the RxLR motif is required for the translocation of Avr3a into the plant cell from P. infestans (Whisson et al., 2007). Additionally, no high-throughput method to transform filamentous oomycete pathogens has been developed to date. Therefore, the well-characterized bacterial T3SS could provide an efficient assay system to deliver oomycete effector proteins to study their cellular functions. It will be fascinating to try to use the T3SS-based effector detector system for studies of fungal as well as oomycete effectors, since there are several advantages of using bacterial T3SS. These include the following facts: (1) a wide range of phytopathogenic bacteria exist, (2) these bacteria are relatively easy to manipulate, and (3) mutant strains of bacterial pathogens such as Pst DC3000 lacking one or more T3S effectors are available. Recently, several shotgun genome sequencing projects of oomycete plant pathogens, such as P. infestans, P. ramorum, P. sojae, P. capsici (genome information of P. capsici will be released soon), and H. parasitica, have been completed (Tyler et al., 2006; http://www.broad.mit.edu/annotation/genome/phytophthora_infestans/Home.html; http://genome.wustl.edu/pub/organism/Fungi/Hyaloperonospora_parasitica/assembly/Hyaloperonospora_parasitica-2.0; http://genome.jgi-psf.org/Physo1_1/Physo1_1.home.html; http://genome.jgi-psf.org/Phyra1_1/Phyra1_1.home.html; http://www.jgi.doe.gov/sequencing/why/CSP2006/Pcapsici.html). Bioinformatic analysis of genome sequences of several Phytophthora spp and H. parasitica revealed several hundred signal peptide and RxLR motif-containing candidate effectors (Tyler et al., 2006; Win et al., 2007). Hitherto, there has been no efficient tool to experimentally confirm true effectors from these oomycete pathogens, apart from generating transgenic plants constitutively or conditionally expressing candidate effectors. Heterologous expression and delivery of the oomycete candidate effectors by bacterial pathogens to test their avirulence as well as virulence functions in host cells would accelerate such analysis. Some of the oomycete or fungal effectors may share common functions with bacterial effectors and therefore cannot be assayed properly using a bacterial strain that carries an effector(s) with a similar function. However, this problem can be solved using a reference strain, such as Pst DC3000, in which many single or multiple effector knockout strains are available. In addition, various approaches to reveal the diversity and functions of bacterial effectors will increase our chances to precisely test oomycete or fungal effectors using the EDV system.

Possible Roles of ATR1 and ATR13 in H. parasitica Pathogenesis in Arabidopsis

Oomycete-derived Nep1 (for necrosis and ethylene-inducing peptide1)-like proteins (NLPs) have been found to trigger various immune responses, including the activation of mitogen-activated protein kinase, callose deposition, and production of nitric oxide and ethylene, in Arabidopsis (Qutob et al., 2006). Two fungal effectors, Avra10 and AvrK1, from the obligate biotrophic fungal pathogen of barley (Hordeum vulgare), Blumeria graminis f sp hordei, have been cloned and found to enhance infection rates of the pathogen in susceptible host plants lacking corresponding R genes (Ridout et al., 2006). For H. parasitica, due to limited accessibility for genetic manipulation of the pathogen, it has not been demonstrated that secreted proteins contribute to the pathogen virulence by suppressing host immunity. Since avirulent and virulent alleles of both ATR1 and ATR13 enhance the virulence of Pst DC3000 on susceptible hosts, it is reasonable to propose that ATR1 and ATR13 may promote Arabidopsis susceptibility by targeting host proteins that are involved in resistance to both bacterial and oomycete pathogens. It seems likely that effector functions of ATR1 and ATR13 are not identical to those of Pst DC3000 effectors, since both enhance the in planta growth of wild-type bacteria in susceptible Arabidopsis accessions. These results were surprising because Pst DC3000, which carries >30 effector proteins, is already a highly virulent bacterial pathogen (Collmer et al., 2002; Buell et al., 2003). Moreover, ATR13-mediated suppression of callose deposition at Arabidopsis cell walls suggests that ATR13 may play a role in suppressing PTI activated by oomycete elicitors such as Nep1-like proteins or CBEL (Gaulin et al., 2006; Qutob et al., 2006). ATR13-mediated callose suppression is the first indication to date of the suppression of host PTI by an oomycete effector, and this suggests that the molecular functions of bacterial and oomycete effectors can overlap. Qutob et al. (2006) showed that there is a significant overlap between Arabidopsis genes that are upregulated by treatments of synthetic peptide derived from bacterial flagellin, flg22, and purified Nep1-like protein from Phytophthora parasitica, indicating that downstream signaling networks activated by bacterial and oomycete PAMPs at least partially overlap. In addition, Arabidopsis lines carrying mutations at plant innate immune signaling components, such as enhanced disease susceptibility1, show enhanced susceptibility to both Pst DC3000 and H. parasitica (Aarts et al., 1998). Together, these data suggest that bacterial and oomycete effectors may suppress host immune networks by targeting plant proteins positioned in defense signaling pathways that are activated by both bacterial and oomycete pathogens. A Pst DC3000 effector, HopM1, suppresses callose deposition and enhances bacterial virulence by degrading At MIN7 protein via the Arabidopsis proteasome (Nomura et al., 2006). Although ATR13 has a suppressor function of PAMP-induced callose deposition similar to HopM1, it seems likely that ATR13 and HopM1 have distinct modes of action in Arabidopsis, because the delivery of ATR13 by Pst DC3000 still enhances the growth of the bacteria carrying functional HopM1. Therefore, ATR13 may target defense components other than At MIN7 that are involved in cell wall–based defense in Arabidopsis. In addition, transient expression of ATR13Maks9 suppressed flg22-triggered ROS production within 20 min, suggesting that the virulence function of ATR13 might be the suppression of early steps in PTI signaling (Figure 7B). These findings suggest that multiple alleles of ATR13 contain common virulence functions that have evolved during interaction with host plants.

Molecular Evolution of H. parasitica Virulence to Suppress Host Immunity

It is now apparent that pathogens have evolved to overcome plant innate immunity triggered by direct or indirect recognition of pathogen molecules by plant extracellular or intracellular receptors (Jones and Dangl, 2006). In bacterial pathogens, especially P. syringae strains, proteins that are secreted to host plant cells, so-called T3SS effectors, are key factors for suppressing host immunity and bacterial survival (Jin et al., 2003). Our T3SS-based analysis of effector functions of ATR13 and phenotypic analysis of H. parasitica isolates in Arabidopsis accessions Col-0, Nd-0, and Ws-0 enabled us to learn more about the molecular evolution of H. parasitica virulence, particularly effectors, during its coevolution with Arabidopsis. ATR13 has undergone diversifying selection processes during H. parasitica–Arabidopsis coevolution (Allen et al., 2004). There are several hypotheses that could explain our paradoxical observations that H. parasitica Emco5 causes disease on Ws-0, yet ATR13Emco5 is recognized by Ws-0. Conceivably, ATR13 protein is not expressed during the infection process on Ws-0. However, this is unlikely, because at least ATR13Emco5 mRNA was expressed (Figure 8B). Another possibility is that not enough ATR13Emco5 protein is delivered to trigger immunity. This is possible if translocation of H. parasitica effectors to plant cell cytoplasm requires plant extracellular components, which can vary among Arabidopsis accessions. Nevertheless, this hypothesis is also inconsistent with our observation that Ws-0 is highly susceptible to H. parasitica Emco5, because it would be very difficult for the pathogen to successfully colonize host plant cells without delivering its effectors. Since the compatibility of pathogens on host plants is the outcome of a complex interaction between pathogen and host factors, the susceptible phenotype of a host plant to a given pathogen can be due not only to the absence of an avirulence effector protein(s) but also to the presence of an ETI suppressor(s), a hypothesis that is supported in the literature. The presence of inhibitor genes was initially suggested in studies on flax rust fungus (Melampsora lini) on flax (Linum usitatissimum) (Jones, 1988). In P. syringae pv phaseolicola, it was shown that the presence of AvrPphC converts a normally avirulent strain to virulence by interfering with AvrPphF-triggered immunity (Tsiamis et al., 2000). Moreover, VirPphA and AvrPtoB have been shown to have suppressor functions on several Avr-triggered HRs (Jackson et al., 1999; Abramovitch et al., 2003). It was also shown that the ETI suppressor function of AvrPtoB requires E3-ligase activity (Abramovitch et al., 2006; Janjusevic et al., 2006). Suppression of AvrRpm1-triggered immunity by another Pseudomonas effector, AvrRpt2 (Ritter and Dangl, 1996), was later shown to be due to direct proteolysis of RIN4 by AvrRpt2 (Mackey et al., 2003). Previously, it was found that the Emco5 allele of ATR1NdWsB is recognized by Arabidopsis accession Ws-0, which is susceptible to H. parasitica Emco5, indicating the presence of an ETI suppressor in H. parasitica Emco5 (Rehmany et al., 2005). Therefore, we favor the hypothesis that H. parasitica Emco5 carries one or more suppressors that are able to convert normally avirulent H. parasitica carrying the Emco5 allele of ATR1 or ATR13 to virulence on Ws-0. It will be interesting to test whether such putative suppressors can be identified among the H. parasitica Emco5 effector complement.

METHODS

Construction of pEDV3

Standard PCR techniques were used to create pEDV3. The multiple cloning site (MCS) of a broad host range vector, pBBR 1MCS-5 (Kovach et al., 1995), was modified by inserting two annealed oligonucleotides, 5′-GAAGCTTATCGATGAATTCCAGCT-3′ and 5′-GGAATTCATCGATAAGCTTCGTAC-3′, at KpnI and SstI sites to create mpBBR 1MCS-5 (MCS was replaced by HindIII-ClaI-EcoRI). A total of 128 bp (from ATG) of the AvrRPS4 promoter and the first 408 bp of the AvrRPS4 open reading frame was amplified from pV316-1A (Hinsch and Staskawicz, 1996) by standard PCR using KS_96 (5′-CCCAAGCTTTTTCCCCGAAGATTAGGAACTGTC-3′) and KS_107 (5′-GGAATTCTTATGCGTAGTCTGGTACGTCGTACGGATAGGATCCGTCGACTCGTTTACCTCCACCCAATAGG-3′) primers to create a DNA fragment containing 5′-HindIII-AvrRPS4N-SalI-BamHI-HA-EcoRI-3′. The amplified DNA fragment was digested with HindIII and EcoRI and purified from a 1.0% agarose gel to ligate with HindIII- and EcoRI-treated mpBBR 1MCS-5. The resulting plasmid was named pEDV3. The sequence of the inserted DNA fragment was confirmed by sequencing with M13F and M13R primers.

AvrRpt2 and ATR13 Plasmid Constructions

The DNA fragment encoding AvrRpt272-255 was amplified from plasmid pVSP61 (own promoter:AvrRpt2; a gift from B.J. Staskawicz) using KS_115 (5′-ACGCGTCGACGGGTGGTTCAAAAAGAAATCATCTA-3′) and KS_116 (5′-CGGGATCCGCGGTAGAGCATTGCGTGTG-3′) primers to have SalI and BamHI at the 5′ and 3′ ends, respectively. DNA fragments encoding Embedded Image, Embedded Image, Embedded Image, Embedded Image, Embedded Image, and Embedded Image were amplified from DNA of Hyaloperonospora parasitica Emoy2 or Emco5 spores as templates using standard PCR conditions to have SalI and BamHI at the 5′ and 3′ ends, respectively. Amplified AvrRpt2 and ATR13 fragments were digested with SalI and BamHI and cloned in SalI- and BamHI-treated pEDV3 to create AvrRpt272-255-pEDV3, Embedded Image (KS_108 [5′-ACGCGTCGACGCAGCCGCCAGCGAAGT-3′] and KS_110 [5′-CGGGATCCCTGACTGGCAACGGCAGTCT-3′]), and Embedded Image (KS_108 and KS_109 [5′-CGGGATCCCTGTCTGTCAAGAGCATCCCGA-3′]). DNA sequences of these constructs were verified by sequencing inserted DNA fragments using M13F and M13R primers.

Bacterial Strains and Plasmid Mobilizations

Bacterial strains used in this study were as follows: Escherichia coli DH5α, Pseudomonas syringae pv tomato DC3000 wild type, ΔhrcC, and ΔCEL (Yuan and He, 1996; Alfano et al., 2000). E. coli DH5α and Pst DC3000 strains were grown in low-salt Luria-Bertani broth at 37 and 28°C, respectively. Pst DC3000 cultures were centrifuged to recover bacteria, washed briefly, and resuspended in sterile water for infection assays. Plasmids were mobilized from E. coli DH5α to wild-type or mutant Pst DC3000 strains by standard triparental matings using E. coli HB101 (pRK2013) as a helper strain.

RNA Expression Analysis

Total RNA was extracted from H. parasitica Emco5–infected 2-week-old Arabidopsis thaliana seedlings from accessions Col-0, Nd-0, and Ws-0 at 48 h after inoculation or from transgenic Col-5 (Dex-ATR13Maks9) plants after 24 h of dexamethasone treatment (20 μM) using TRIZOL reagent (Invitrogen) following the instructions from the manufacturer. First-strand cDNA was synthesized using SuperScript II reverse transcriptase (Invitrogen) and the oligo(dT) primer. PCR (39 cycles for ATR13Emco5 and MTD, 25 cycles for ATR13Maks9 and At Actin) was performed using Taq DNA polymerase (Qiagen). Primers used for PCR were KS_271 (5′-GCAGCCGCCAGCGAAGTAT-3′) and KS_272 (5′-CTGTCTGTCAAGAGCATCCCGAAG-3′) primers for ATR13Emco5, MTD1 (5′-GACCCGGCTGCGAAGAAGTATGC-3′) and MTD2 (5′-CCAGCGGCCGACCAACAATG-3′) for MTD, KS_271 (5′-GCAGCCGCCAGCGAAGTAT-3′) and KS_168 (5′-CGGGATCCTACACTGGCATACTGGATGGGGG-3′) for ATR13Maks9, and AC1 (5′-ATGGCAGACGGTGAGGATATTCA-3′) and AC2 (5′-GCCTTTGCAATCCACATCTGTTTG-3′) for At Actin (At5g09810) amplifications.

Protein Analysis

Frozen plant tissue was ground and mixed with an equal volume of cold protein isolation buffer (20 mm Tris-HCl, pH 7.5, 1 mm EDTA, pH 8.0, 5 mm DTT, 150 mm NaCl, 1% Triton X-100, 0.1% SDS, 10% glycerol, and 1× Protease Inhibitor Cocktail [Sigma-Aldrich]). The mixture was spun down, the supernatant was transferred to a new tube, and SDS loading buffer (300 mm Tris-HCl, pH 6.8, 8.7% SDS, 5% β-mercaptoethanol, 30% glycerol, and 0.12 mg/mL bromophenol blue) was added. Proteins were separated by SDS-PAGE, electroblotted onto a polyvinylidene difluoride membrane (Bio-Rad), and probed with horseradish peroxidase–conjugated anti-HA antibody (Sigma-Aldrich). Bands were visualized using ECL+ (Amersham).

Pst DC3000 and H. parasitica Pathology Experiments

Five-week-old Arabidopsis plants were hand-infiltrated with 5 × 107 or 5 × 105 cfu/mL Pst DC3000 suspensions for HR or in planta growth assays, respectively. HR symptoms were scored at 22 h after infection, and in planta bacterial growth was measured at 0 and 3 (or 4) d after infiltration by extracting bacteria from leaf discs and plating a series of dilutions on the medium supplemented with appropriate antibiotics. Disease symptoms were observed until 7 d after infiltration. For H. parasitica Emco5 infection, 2-week-old plants were sprayed with a spore suspension at a concentration of 105 spores/mL. Plants were kept in a growth cabinet at 17°C for 7 to 10 d with a 10-h/14-h day/night cycle.

Callose Staining and Microscopic Analysis

Leaves of 5-week-old Arabidopsis accession Col-0 plants were hand-infiltrated with 1 × 108 cfu/mL Pst DC3000 suspensions, and leaf samples were taken at 12 to 14 h after inoculation. For transgenic Col-5 (Dex-ATR13Maks9) plants, 4-week-old leaves were sprayed with dexamethasone (20 μM) and subsequently hand-infiltrated with flg22 (1 μM) after 24 h. Samples were taken at 8 h after flg22 treatment. A total of eight leaf discs were taken from eight leaves for callose staining. Harvested leaf samples were cleared three times (overnight incubation at room temperature) with 100% methanol, washed three times (2 h for each washing) with sterilized water, and stained with aniline blue (0.05% in phosphate buffer, pH 8.0) for 24 h. Leaf samples stained with aniline blue were examined with a Zeiss Axiophot photomicroscope using an A3 fluorescence cube, and the images were analyzed using Image-Pro Plus software (Media Cybernetics).

Measurement of ROS Production

A total of 96 leaf discs from 5-week-old Col-5 (Dex-ATR13Maks9) plants were taken and floated on water or 20 μM dexamethasone solution for 19 h prior to flg22 treatment. ROS production was measured by adding luminol (final concentration, 20 μM; Sigma-Aldrich), 1 μg of horseradish peroxidase (Sigma-Aldrich), and flg22 (final concentration, 100 nM) to a 96-well plate containing leaf discs (48 samples for each pretreatment). Luminescence was measured every 2 min for at least 60 min after treatment with flg22 using the Photek camera system.

Accession Numbers

Sequence data from this article can be found in the GenBank/EMBL data libraries under the following accession numbers: AJ870977 (avrRpt2), L43559 (avrRps4), AY842877 (ATR1Emoy2), AY842883 (ATR1Cala2), AY785302 (ATR13Emoy2), and AY785305 (ATR13Emco5).

Supplemental Data

The following materials are available in the online version of this article.

  • Supplemental Figure 1. Test of pBBR 1MCS-5 and pEDV3 on Bacterial Growth.

  • Supplemental Figure 2. Delivery of AvrRPS4N-AvrRpt2-HA Confers RPS2-Dependent Resistance against Pst DC3000 in Arabidopsis Accession Col-0.

  • Supplemental Figure 3. AvrRPS4N-ATR13Emco5-HA Is Not Processed When Expressed in Pst DC3000.

  • Supplemental Figure 4. The C-Terminal HA Epitope Tag Does Not Interfere with ATR1Emoy2-Triggered HR When Delivered by Pst DC3000.

Acknowledgments

We thank J. Beynon (University of Warwick) for transgenic Col-5 (Dex-ATR13Maks9) and Col-5 (RPP13-Nd) seeds, S.Y. He (Michigan State University) for Pst DC3000 ΔCEL mutant strains, J. Dangl (University of North Carolina) for rps2-101c mutant seeds, and C. Segonzac (Sainsbury Laboratory) for technical help to measure ROS burst. We are also grateful to B. Staskawicz (University of California, Berkeley) for Pst DC3000 wild type and ΔhrcC mutant strains and for helpful discussions about engineering type III delivery of oomycete effectors. This work was funded by the Gatsby Charitable Foundation.

Footnotes

  • The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Jonathan D.G. Jones (jonathan.jones{at}tsl.ac.uk).

  • www.plantcell.org/cgi/doi/10.1105/tpc.107.054262

  • ↵[W] Online version contains Web-only data.

  • Received September 14, 2007.
  • Revised November 27, 2007.
  • Accepted December 11, 2007.
  • Published December 28, 2007.

References

  1. ↵
    Aarts, N., Metz, M., Holub, E., Staskawicz, B.J., Daniels, M.J., and Parker, J.E. (1998). Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis. Proc. Natl. Acad. Sci. USA 95: 10306–10311.
    OpenUrlAbstract/FREE Full Text
  2. ↵
    Abramovitch, R.B., Janjusevic, R., Stebbins, C.E., and Martin, G.B. (2006). Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Proc. Natl. Acad. Sci. USA 103: 2851–2856.
    OpenUrlAbstract/FREE Full Text
  3. ↵
    Abramovitch, R.B., Kim, Y.J., Chen, S.R., Dickman, M.B., and Martin, G.B. (2003). Pseudomonas type III effector AvrPtoB induces plant disease susceptibility by inhibition of host programmed cell death. EMBO J. 22: 60–69.
    OpenUrlAbstract
  4. ↵
    Ade, J., DeYoung, B.J., Golstein, C., and Innes, R.W. (2007). Indirect activation of a plant nucleotide binding site-leucine-rich repeat protein by a bacterial protease. Proc. Natl. Acad. Sci. USA 104: 2531–2536.
    OpenUrlAbstract/FREE Full Text
  5. ↵
    Akeda, Y., and Galan, J.E. (2005). Chaperone release and unfolding of substrates in type III secretion. Nature 437: 911–915.
    OpenUrlCrossRefPubMed
  6. ↵
    Alfano, J.R., Charkowski, A.O., Deng, W.L., Badel, J.L., Petnicki-Ocwieja, T., van Dijk, K., and Collmer, A. (2000). The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic structure composed of a cluster of type III secretion genes bounded by exchangeable effector and conserved effector loci that contribute to parasitic fitness and pathogenicity in plants. Proc. Natl. Acad. Sci. USA 97: 4856–4861.
    OpenUrlAbstract/FREE Full Text
  7. ↵
    Allen, R.L., Bittner-Eddy, P.D., Grenvitte-Briggs, L.J., Meitz, J.C., Rehmany, A.P., Rose, L.E., and Beynon, J.L. (2004). Host-parasite coevolutionary conflict between Arabidopsis and downy mildew. Science. 306: 1957–1960.
    OpenUrlAbstract/FREE Full Text
  8. ↵
    Armstrong, M.R., et al. (2005). An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm. Proc. Natl. Acad. Sci. USA 102: 7766–7771.
    OpenUrlAbstract/FREE Full Text
  9. ↵
    Axtell, M.J., and Staskawicz, B.J. (2003). Initiation of RPS2-specified disease resistance in Arabidopsis is coupled to the AvrRpt2-directed elimination of RIN4. Cell 112: 369–377.
    OpenUrlCrossRefPubMed
  10. ↵
    Bhattacharjee, S., Hiller, N.L., Liolios, K., Win, J., Kanneganti, T.D., Young, C., Kamoun, S., and Haldar, K. (2006). The malarial host-targeting signal is conserved in the Irish potato famine pathogen. PLoS Pathog. 2: e50.
    OpenUrlCrossRefPubMed
  11. ↵
    Birch, P.R., Rehmany, A.P., Pritchard, L., Kamoun, S., and Beynon, J.L. (2006). Trafficking arms: Oomycete effectors enter host plant cells. Trends Microbiol. 14: 8–11.
    OpenUrlCrossRefPubMed
  12. ↵
    Bittner-Eddy, P., Can, C., Gunn, N., Pinel, M., Tor, M., Crute, I., Holub, E.B., and Beynon, J. (1999). Genetic and physical mapping of the RPP13 locus, in Arabidopsis, responsible for specific recognition of several Peronospora parasitica (downy mildew) isolates. Mol. Plant Microbe Interact. 12: 792–802.
    OpenUrlCrossRefPubMed
  13. ↵
    Bittner-Eddy, P.D., Crute, I.R., Holub, E.B., and Beynon, J.L. (2000). RPP13 is a simple locus in Arabidopsis thaliana for alleles that specify downy mildew resistance to different avirulence determinants in Peronospora parasitica. Plant J. 21: 177–188.
    OpenUrlCrossRefPubMed
  14. ↵
    Botella, M.A., Parker, J.E., Frost, L.N., Bittner-Eddy, P.D., Beynon, J.L., Daniels, M.J., Holub, E.B., and Jones, J.D. (1998). Three genes of the Arabidopsis RPP1 complex resistance locus recognize distinct Peronospora parasitica avirulence determinants. Plant Cell 10: 1847–1860.
    OpenUrlAbstract/FREE Full Text
  15. ↵
    Buell, C.R., et al. (2003). The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000. Proc. Natl. Acad. Sci. USA 100: 10181–10186.
    OpenUrlAbstract/FREE Full Text
  16. ↵
    Buttner, D., and Bonas, U. (2006). Who comes first? How plant pathogenic bacteria orchestrate type III secretion. Curr. Opin. Microbiol. 9: 193–200.
    OpenUrlCrossRefPubMed
  17. ↵
    Chisholm, S.T., Coaker, G., Day, B., and Staskawicz, B.J. (2006). Host-microbe interactions: Shaping the evolution of the plant immune response. Cell 124: 803–814.
    OpenUrlCrossRefPubMed
  18. ↵
    Collmer, A., Lindeberg, M., Petnicki-Ocwieja, T., Schneider, D.J., and Alfano, J.R. (2002). Genomic mining type III secretion system effectors in Pseudomonas syringae yields new picks for all TTSS prospectors. Trends Microbiol. 10: 462–469.
    OpenUrlCrossRefPubMed
  19. ↵
    Cornelis, G.R. (2006). The type III secretion injectisome. Nat. Rev. Microbiol. 4: 811–825.
    OpenUrlCrossRefPubMed
  20. ↵
    DebRoy, S., Thilmony, R., Kwack, Y.B., Nomura, K., and He, S.Y. (2004). A family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants. Proc. Natl. Acad. Sci. USA 101: 9927–9932.
    OpenUrlAbstract/FREE Full Text
  21. ↵
    Deslandes, L., Olivier, J., Peeters, N., Feng, D.X., Khounlotham, M., Boucher, C., Somssich, L., Genin, S., and Marco, Y. (2003). Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc. Natl. Acad. Sci. USA 100: 8024–8029.
    OpenUrlAbstract/FREE Full Text
  22. ↵
    Dodds, P.N., Lawrence, G.J., Catanzariti, A.-M., Teh, T., Wang, C.-I.A., Ayliffe, M.A., Kobe, B., and Ellis, J.G. (2006). Direct protein interaction underlies gene-for-gene specificity and co-evolution of the flax resistance genes and flax rust avirulence genes. Proc. Natl. Acad. Sci. USA 103: 8888–8893.
    OpenUrlAbstract/FREE Full Text
  23. ↵
    Felix, G., Duran, J.D., Volko, S., and Boller, T. (1999). Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J. 18: 265–276.
    OpenUrlCrossRefPubMed
  24. ↵
    Fu, Y., and Galan, J.E. (1998). Identification of a specific chaperone for SptP, a substrate of the centisome 63 type III secretion system of Salmonella typhimurium. J. Bacteriol. 180: 3393–3399.
    OpenUrlAbstract/FREE Full Text
  25. ↵
    Fu, Z.Q., Guo, M., Jeong, B.R., Tian, F., Elthon, T.E., Cerny, R.L., Staiger, D., and Alfano, J.R. (2007). A type III effector ADP-ribosylates RNA-binding proteins and quells plant immunity. Nature 447: 284–288.
    OpenUrlCrossRefPubMed
  26. ↵
    Gaulin, E., et al. (2006). Cellulose binding domains of a Phytophthora cell wall protein are novel pathogen-associated molecular patterns. Plant Cell 18: 1766–1777.
    OpenUrlAbstract/FREE Full Text
  27. ↵
    Grant, S.R., Fisher, E.J., Chang, J.H., Mole, B.M., and Dangl, J.L. (2006). Subterfuge and manipulation: Type III effector proteins of phytopathogenic bacteria. Annu. Rev. Microbiol. 60: 425–449.
    OpenUrlCrossRefPubMed
  28. ↵
    Guttman, D.S., Vinatzer, B.A., Sarkar, S.F., Ranall, M.V., Kettler, G., and Greenberg, J.T. (2002). A functional screen for the type III (Hrp) secretome of the plant pathogen Pseudomonas syringae. Science. 295: 1722–1726.
    OpenUrlAbstract/FREE Full Text
  29. ↵
    Haldar, K., Kamoun, S., Hiller, N.L., Bhattacharje, S., and van Ooij, C. (2006). Common infection strategies of pathogenic eukaryotes. Nat. Rev. Microbiol. 4: 922–931.
    OpenUrlCrossRefPubMed
  30. ↵
    Hauck, P., Thilmony, R., and He, S.Y. (2003). A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants. Proc. Natl. Acad. Sci. USA 100: 8577–8582.
    OpenUrlAbstract/FREE Full Text
  31. ↵
    Hiller, N.L., Bhattacharjee, S., van Ooij, C., Liolios, K., Harrison, T., Lopez-Estrano, C., and Haldar, K. (2004). A host-targeting signal in virulence proteins reveals a secretome in malarial infection. Science. 306: 1934–1937.
    OpenUrlAbstract/FREE Full Text
  32. ↵
    Hinsch, M., and Staskawicz, B. (1996). Identification of a new Arabidopsis disease resistance locus, RPs4, and cloning of the corresponding avirulence gene, avrRps4, from Pseudomonas syringae pv. pisi. Mol. Plant Microbe Interact. 9: 55–61.
    OpenUrlCrossRefPubMed
  33. ↵
    Innes, R.W., Bent, A.F., Kunkel, B.N., Bisgrove, S.R., and Staskawicz, B.J. (1993). Molecular analysis of avirulence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringae avirulence genes. J. Bacteriol. 175: 4859–4869.
    OpenUrlAbstract/FREE Full Text
  34. ↵
    Jackson, R.W., Athanassopoulos, E., Tsiamis, G., Mansfield, J.W., Sesma, A., Arnold, D.L., Gibbon, M.J., Murillo, J., Taylor, J.D., and Vivian, A. (1999). Identification of a pathogenicity island, which contains genes for virulence and avirulence, on a large native plasmid in the bean pathogen Pseudomonas syringae pathovar phaseolicola. Proc. Natl. Acad. Sci. USA 96: 10875–10880.
    OpenUrlAbstract/FREE Full Text
  35. ↵
    Janjusevic, R., Abramovitch, R.B., Martin, G.B., and Stebbins, C.E. (2006). A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase. Science. 311: 222–226.
    OpenUrlAbstract/FREE Full Text
  36. ↵
    Jia, Y., McAdams, S.A., Bryan, G.T., Hershey, H.P., and Valent, B. (2000). Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. EMBO J. 19: 4004–4014.
    OpenUrlAbstract
  37. ↵
    Jin, Q., Thilmony, R., Zwiesler-Vollick, J., and He, S.Y. (2003). Type III protein secretion in Pseudomonas syringae. Microbes Infect. 5: 301–310.
    OpenUrlCrossRefPubMed
  38. ↵
    Jones, D.A. (1988). Genetic properties of inhibitor genes in flax rust that alter avirulence to virulence on flax. Phytopathology 78: 342–344.
    OpenUrlCrossRef
  39. ↵
    Jones, J.D., and Dangl, J.L. (2006). The plant immune system. Nature 444: 323–329.
    OpenUrlCrossRefPubMed
  40. ↵
    Kamoun, S. (2006). A catalogue of the effector secretome of plant pathogenic oomycetes. Annu. Rev. Phytopathol. 44: 41–60.
    OpenUrlCrossRefPubMed
  41. ↵
    Kim, M.G., da Cunha, L., McFall, A.J., Belkhadir, Y., DebRoy, S., Dangl, J.L., and Mackey, D. (2005). Two Pseudomonas syringae type III effectors inhibit RIN4-regulated basal defense in Arabidopsis. Cell 121: 749–759.
    OpenUrlCrossRefPubMed
  42. ↵
    Kovach, M.E., Elzer, P.H., Hill, D.S., Robertson, G.T., Farris, M.A., Roop, R.M., II, and Peterson, K.M. (1995). Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166: 175–176.
    OpenUrlCrossRefPubMed
  43. ↵
    Lee, S.H., and Galan, J.E. (2004). Salmonella type III secretion-associated chaperones confer secretion-pathway specificity. Mol. Microbiol. 51: 483–495.
    OpenUrlCrossRefPubMed
  44. ↵
    Lindgren, P.B., Peet, R.C., and Panopoulos, N.J. (1986). Gene cluster of Pseudomonas syringae pv. “phaseolicola” controls pathogenicity of bean plants and hypersensitivity of nonhost plants. J. Bacteriol. 168: 512–522.
    OpenUrlAbstract/FREE Full Text
  45. ↵
    Mackey, D., Belkhadir, Y., Alonso, J.M., Ecker, J.R., and Dangl, J.L. (2003). Arabidopsis RIN4 is a target of the type III virulence effector AvrRpt2 and modulates RPS2-mediated resistance. Cell 112: 379–389.
    OpenUrlCrossRefPubMed
  46. ↵
    Mackey, D., Holt, B.F., Wiig, A., and Dangl, J.L. (2002). RIN4 interacts with Pseudomonas syringae type III effector molecules and is required for RPM1-mediated resistance in Arabidopsis. Cell 108: 743–754.
    OpenUrlCrossRefPubMed
  47. ↵
    Mudgett, M.B., Chesnokova, O., Dahlbeck, D., Clark, E.T., Rossier, O., Bonas, U., and Staskawicz, B.J. (2000). Molecular signals required for type III secretion and translocation of the Xanthomonas campestris AvrBs2 protein to pepper plants. Proc. Natl. Acad. Sci. USA 97: 13324–13329.
    OpenUrlAbstract/FREE Full Text
  48. ↵
    Mudgett, M.B., and Staskawicz, B.J. (1999). Characterization of the Pseudomonas syringae pv. tomato AvrRpt2 protein: Demonstration of secretion and processing during bacterial pathogenesis. Mol. Microbiol. 32: 927–941.
    OpenUrlCrossRefPubMed
  49. ↵
    Nomura, K., Debroy, S., Lee, Y.H., Pumplin, N., Jones, J., and He, S.Y. (2006). A bacterial virulence protein suppresses host innate immunity to cause plant disease. Science. 313: 220–223.
    OpenUrlAbstract/FREE Full Text
  50. ↵
    Petnicki-Ocwieja, T., Schneider, D.J., Tam, V.C., Chancey, S.T., Shan, L., Jamir, Y., Schechter, L.M., Janes, M.D., Buell, C.R., Tang, X., Collmer, A., and Alfano, J.R. (2002). Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000. Proc. Natl. Acad. Sci. USA 99: 7652–7657.
    OpenUrlAbstract/FREE Full Text
  51. ↵
    Qutob, D., et al. (2006). Phytotoxicity and innate immune responses induced by Nep1-like proteins. Plant Cell 18: 3721–3744.
    OpenUrlAbstract/FREE Full Text
  52. ↵
    Rehmany, A.P., Gordon, A., Rose, L.E., Allen, R.L., Armstrong, M.R., Whisson, S.C., Kamoun, S., Tyler, B.M., Birch, P.R.J., and Beynon, J.L. (2005). Differential recognition of highly divergent downy mildew avirulence gene alleles by RPP1 resistance genes from two Arabidopsis lines. Plant Cell 17: 1839–1850.
    OpenUrlAbstract/FREE Full Text
  53. ↵
    Rehmany, A.P., Grenville, L.J., Gunn, N.D., Allen, R.L., Paniwnyk, Z., Byrne, J., Whisson, S.C., Birch, P.R.J., and Beynon, J.L. (2003). A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd. Fungal Genet. Biol. 38: 33–42.
    OpenUrlCrossRefPubMed
  54. ↵
    Ridout, C.J., Skamnioti, P., Porritt, O., Sacristan, S., Jones, J.D., and Brown, J.K. (2006). Multiple avirulence paralogues in cereal powdery mildew fungi may contribute to parasite fitness and defeat of plant resistance. Plant Cell 18: 2402–2414.
    OpenUrlAbstract/FREE Full Text
  55. ↵
    Ritter, C., and Dangl, J.L. (1996). Interference between two specific pathogen recognition events mediated by distinct plant disease resistance genes. Plant Cell 8: 251–257.
    OpenUrlAbstract/FREE Full Text
  56. ↵
    Roden, J.A., Belt, B., Ross, J.B., Tachibana, T., Vargas, J., and Mudgett, M.B. (2004). A genetic screen to isolate type III effectors translocated into pepper cells during Xanthomonas infection. Proc. Natl. Acad. Sci. USA 101: 16624–16629.
    OpenUrlAbstract/FREE Full Text
  57. ↵
    Ronald, P.C., Salmeron, J.M., Carland, F.M., and Staskawicz, B.J. (1992). The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene. J. Bacteriol. 174: 1604–1611.
    OpenUrlAbstract/FREE Full Text
  58. ↵
    Rose, L.E., Bittner-Eddy, P.D., Langley, C.H., Holub, E.B., Michelmore, R.W., and Beynon, J.L. (2004). The maintenance of extreme amino acid diversity at the disease resistance gene, RPP13, in Arabidopsis thaliana. Genetics 166: 1517–1527.
    OpenUrlAbstract/FREE Full Text
  59. ↵
    Shan, W.X., Cao, M., Dan, L.U., and Tyler, B.M. (2004). The Avr1b locus of Phytophthora sojae encodes an elicitor and a regulator required for avirulence on soybean plants carrying resistance gene Rps1b. Mol. Plant Microbe Interact. 17: 394–403.
    OpenUrlCrossRefPubMed
  60. ↵
    Stebbins, C.E., and Galan, J.E. (2001). Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion. Nature 414: 77–81.
    OpenUrlCrossRefPubMed
  61. ↵
    Tsiamis, G., Mansfield, J.W., Hockenhull, R., Jackson, R.W., Sesma, A., Athanassopoulos, E., Bennett, M.A., Stevens, C., Vivian, A., Taylor, J.D., and Murillo, J. (2000). Cultivar-specific avirulence and virulence functions assigned to avrPphF in Pseudomonas syringae pv. phaseolicola, the cause of bean halo-blight disease. EMBO J. 19: 3204–3214.
    OpenUrlAbstract/FREE Full Text
  62. ↵
    Tyler, B.M., et al. (2006). Phytophthora genome sequences uncover evolutionary origins and mechanisms of pathogenesis. Science 313: 1261–1266.
    OpenUrlAbstract/FREE Full Text
  63. ↵
    Vinatzer, B.A., Teitzel, G.M., Lee, M.W., Jelenska, J., Hotton, S., Fairfax, K., Jenrette, J., and Greenberg, J.T. (2006). The type III effector repertoire of Pseudomonas syringae pv. syringae B728a and its role in survival and disease on host and non-host plants. Mol. Microbiol. 62: 26–44.
    OpenUrlCrossRefPubMed
  64. ↵
    Whisson, S.C., et al. (2007). A translocation signal for delivery of oomycete effector proteins into host plant cells. Nature 450: 115–118.
    OpenUrlCrossRefPubMed
  65. ↵
    Win, J., Morgan, W., Bos, J., Krasileva, K.V., Cano, L.M., Chaparro-Garcia, A., Ammar, R., Staskawicz, B.J., and Kamoun, S. (2007). Adaptive evolution has targeted the C-terminal domain of the RXLR effectors of plant pathogenic oomycetes. Plant Cell 19: 2349–2369.
    OpenUrlAbstract/FREE Full Text
  66. ↵
    Yuan, J., and He, S.Y. (1996). The Pseudomonas syringae Hrp regulation and secretion system controls the production and secretion of multiple extracellular proteins. J. Bacteriol. 178: 6399–6402.
    OpenUrlAbstract/FREE Full Text
  67. ↵
    Zhang, J., et al. (2007). A Pseudomonas syringae effector inactivates MAPKs to suppress PAMP-induced immunity in plants. Cell Host Microbe 1: 175–185.
    OpenUrlCrossRefPubMed
  68. ↵
    Zipfel, C., Kunze, G., Chinchilla, D., Caniard, A., Jones, J.D., Boller, T., and Felix, G. (2006). Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation. Cell 125: 749–760.
    OpenUrlCrossRefPubMed
  69. ↵
    Zipfel, C., Robatzek, S., Navarro, L., Oakeley, E.J., Jones, J.D., Felix, G., and Boller, T. (2004). Bacterial disease resistance in Arabidopsis through flagellin perception. Nature 428: 764–767.
    OpenUrlCrossRefPubMed

NOTE ADDED IN PROOF

  1. Rentel et al. (2008) have also shown that ATR13 proteins can be delivered to plant cells via type III secretions, in their case after fusion to the AvrRpm1 N terminus, where they also confer reduced bacterial virulence depending on the host genotype.
  2. ↵
    Rentel, M.C., Leonelli, L., Dahlbeck, D., Zhao, B., and Staskawicz, B.J. (2008). Recognition of the Hyaloperonospora parasitica effector ATR13 triggers resistance against oomycete, bacterial, and viral pathogens. Proc. Natl. Acad. Sci. USA, in press.
View Abstract
PreviousNext
Back to top

Table of Contents

Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The Downy Mildew Effector Proteins ATR1 and ATR13 Promote Disease Susceptibility in Arabidopsis thaliana
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
The Downy Mildew Effector Proteins ATR1 and ATR13 Promote Disease Susceptibility in Arabidopsis thaliana
Kee Hoon Sohn, Rita Lei, Adnane Nemri, Jonathan D.G. Jones
The Plant Cell Dec 2007, 19 (12) 4077-4090; DOI: 10.1105/tpc.107.054262

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
The Downy Mildew Effector Proteins ATR1 and ATR13 Promote Disease Susceptibility in Arabidopsis thaliana
Kee Hoon Sohn, Rita Lei, Adnane Nemri, Jonathan D.G. Jones
The Plant Cell Dec 2007, 19 (12) 4077-4090; DOI: 10.1105/tpc.107.054262
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • METHODS
    • Acknowledgments
    • Footnotes
    • References
    • NOTE ADDED IN PROOF
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell Online: 19 (12)
The Plant Cell
Vol. 19, Issue 12
December 2007
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Advertising (PDF)
  • Back Matter (PDF)
  • Front Matter (PDF)
View this article with LENS

More in this TOC Section

  • Regulation of Aluminum Resistance in Arabidopsis Involves the SUMOylation of the Zinc Finger Transcription Factor STOP1
  • Molecular Mechanism Underlying the Synergetic Effect of Jasmonate on Abscisic Acid Signaling during Seed Germination in Arabidopsis
  • Substrate Specificity of LACCASE8 Facilitates Polymerization of Caffeyl Alcohol for C-Lignin Biosynthesis in the Seed Coat of Cleome hassleriana
Show more RESEARCH ARTICLES

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire