Table of Contents
A
Adam, Zach
- You have accessRestricted AccessThe Thylakoid Lumen Protease Deg1 Is Involved in the Repair of Photosystem II from Photoinhibition in ArabidopsisEinat Kapri-Pardes, Leah Naveh, Zach AdamPlant Cell Mar 2007, 19 (3) 1039-1047; DOI: https://doi.org/10.1105/tpc.106.046573
Photosynthesis is accompanied by accumulation of highly oxidizing species and oxygen radicals that cause photodamage within the photosystem II complex. Removal of damaged copies of the D1 protein is a prerequisite for reassembly of this complex. RNAi mutant and other analyses suggest that the lumenal protease Deg1 is involved in degradation of the D1 protein during PSII repair from photoinhibition.
Ali, Rashid
- You have accessRestricted AccessDeath Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate ImmunityRashid Ali, Wei Ma, Fouad Lemtiri-Chlieh, Dimitrios Tsaltas, Qiang Leng, Susannne von Bodman, Gerald A. BerkowitzPlant Cell Mar 2007, 19 (3) 1081-1095; DOI: https://doi.org/10.1105/tpc.106.045096
Plants lacking a Ca2+-conducting channel do not undergo hypersensitive response to pathogens and have impaired nitric oxide generation in response to application of a pathogen-associated molecular pattern elicitor. Patch clamp analysis demonstrated elicitor activation of inward cation currents; this work provides insights regarding early steps in pathogen response signaling in plants.
Anderson, Lisa K.
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Arazi, Tzahi
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Ariizumi, Tohru
- You have accessRestricted AccessSeed Germination of GA-Insensitive sleepy1 Mutants Does Not Require RGL2 Protein Disappearance in ArabidopsisTohru Ariizumi, Camille M. SteberPlant Cell Mar 2007, 19 (3) 791-804; DOI: https://doi.org/10.1105/tpc.106.048009
Proper regulation of seed dormancy and germination is critical to plant survival. It was thought that disappearance of the negative regulator RGA-LIKE2 (RGL2) protein is required for seed germination. This report finds instead that RGL2 accumulates in germinating sleepy1 (sly1) mutants, suggesting that protein disappearance is not the only mechanism that relieves RGL2 repression of seed germination.
Arnold, Maeva
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
Azoulay, Tamar
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
B
Baldwin, Ian T.
- You have accessRestricted AccessHerbivory Rapidly Activates MAPK Signaling in Attacked and Unattacked Leaf Regions but Not between Leaves of Nicotiana attenuataJianqiang Wu, Christian Hettenhausen, Stefan Meldau, Ian T. BaldwinPlant Cell Mar 2007, 19 (3) 1096-1122; DOI: https://doi.org/10.1105/tpc.106.049353
Mitogen-activated protein kinase (MAPK) signaling mediates plant responses to herbivore attack, but the transduction of these signals is poorly understood. This work examines how plants deploy defense reactions and demonstrates that herbivore oral secretions (OS) dramatically amplify wound-induced MAPK activity and that fatty acid–amino acid conjugates in herbivore OS are the elicitors.
Barlet, Xavier
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Barone, Mario
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
Bassi, Roberto
- You have accessRestricted AccessThe Arabidopsis aba4-1 Mutant Reveals a Specific Function for Neoxanthin in Protection against Photooxidative StressLuca Dall'Osto, Stefano Cazzaniga, Helen North, Annie Marion-Poll, Roberto BassiPlant Cell Mar 2007, 19 (3) 1048-1064; DOI: https://doi.org/10.1105/tpc.106.049114
Carotenoids help protect the chloroplast from photoxidative stress. The aba4-1 mutant lacks neoxanthin but retains all other xanthophyll species. This research shows that neoxanthin plays a specific role in protection of Lhc proteins, the PSII reaction center, and thylakoids from photooxidative stress and protects against the damaging effects of reactive oxygen species, particularly superoxide anions.
Ben-Yaakov, Eran
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Berkowitz, Gerald A.
- You have accessRestricted AccessDeath Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate ImmunityRashid Ali, Wei Ma, Fouad Lemtiri-Chlieh, Dimitrios Tsaltas, Qiang Leng, Susannne von Bodman, Gerald A. BerkowitzPlant Cell Mar 2007, 19 (3) 1081-1095; DOI: https://doi.org/10.1105/tpc.106.045096
Plants lacking a Ca2+-conducting channel do not undergo hypersensitive response to pathogens and have impaired nitric oxide generation in response to application of a pathogen-associated molecular pattern elicitor. Patch clamp analysis demonstrated elicitor activation of inward cation currents; this work provides insights regarding early steps in pathogen response signaling in plants.
Berrocal-Lobo, Marta
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Bohne, Alexandra-Viola
- You have accessRestricted AccessArabidopsis Phage-Type RNA Polymerases: Accurate in Vitro Transcription of Organellar GenesKristina Kühn, Alexandra-Viola Bohne, Karsten Liere, Andreas Weihe, Thomas BörnerPlant Cell Mar 2007, 19 (3) 959-971; DOI: https://doi.org/10.1105/tpc.106.046839
Three Arabidopsis nuclear-encoded T7 phage-type RNA polymerases (RNAPs) localize to mitochondria, plastids, or both. This study finds that two of them can initiate transcription from both mitochondrial and plastid promoters in vitro without auxiliary factors. The ability for promoter recognition appears to have been conserved over a long period of evolution of organellar RNAPs.
Börner, Thomas
- You have accessRestricted AccessArabidopsis Phage-Type RNA Polymerases: Accurate in Vitro Transcription of Organellar GenesKristina Kühn, Alexandra-Viola Bohne, Karsten Liere, Andreas Weihe, Thomas BörnerPlant Cell Mar 2007, 19 (3) 959-971; DOI: https://doi.org/10.1105/tpc.106.046839
Three Arabidopsis nuclear-encoded T7 phage-type RNA polymerases (RNAPs) localize to mitochondria, plastids, or both. This study finds that two of them can initiate transcription from both mitochondrial and plastid promoters in vitro without auxiliary factors. The ability for promoter recognition appears to have been conserved over a long period of evolution of organellar RNAPs.
Bradley, Desmond
- You have accessRestricted AccessTERMINAL FLOWER1 Is a Mobile Signal Controlling Arabidopsis ArchitectureLucio Conti, Desmond BradleyPlant Cell Mar 2007, 19 (3) 767-778; DOI: https://doi.org/10.1105/tpc.106.049767
TERMINAL FLOWER1 (TFL1) affects the number and identity of aerial organs. TFL1 mRNA is restricted to the inner cells of the meristem, but this work reveals that the protein is a mobile signal that becomes evenly distributed across the meristem and suggests a model for how floral and shoot meristems establish a feedback loop of expression and protein movement to control inflorescence architecture.
Broger, Larissa
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
C
Cao, Wenguang
- You have accessRestricted AccessThe N-Terminal Double-Stranded RNA Binding Domains of Arabidopsis HYPONASTIC LEAVES1 Are Sufficient for Pre-MicroRNA ProcessingFeijie Wu, Lin Yu, Wenguang Cao, Yanfei Mao, Zhongyuan Liu, Yuke HePlant Cell Mar 2007, 19 (3) 914-925; DOI: https://doi.org/10.1105/tpc.106.048637
Some double-stranded RNA binding proteins participate in miRNA biogenesis. A series of deletion mutants demonstrated that the N-terminal region of HYPONASTIC LEAVES1 with two double-stranded RNA binding domains is sufficient for miRNA biogenesis and capable of rescuing the hyl1 phenotype. This study also showed that the putative NLS is a true nuclear localization signal.
Carrington, James C.
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
Cascón, Tomas
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Castresana, Carmen
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Catarecha, Pablo
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Cazzaniga, Stefano
- You have accessRestricted AccessThe Arabidopsis aba4-1 Mutant Reveals a Specific Function for Neoxanthin in Protection against Photooxidative StressLuca Dall'Osto, Stefano Cazzaniga, Helen North, Annie Marion-Poll, Roberto BassiPlant Cell Mar 2007, 19 (3) 1048-1064; DOI: https://doi.org/10.1105/tpc.106.049114
Carotenoids help protect the chloroplast from photoxidative stress. The aba4-1 mutant lacks neoxanthin but retains all other xanthophyll species. This research shows that neoxanthin plays a specific role in protection of Lhc proteins, the PSII reaction center, and thylakoids from photooxidative stress and protects against the damaging effects of reactive oxygen species, particularly superoxide anions.
Chapman, Elisabeth J.
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
Chen, Rongzhi
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
Chen, Zhixiang
- You have accessRestricted AccessImproperly Terminated, Unpolyadenylated mRNA of Sense Transgenes Is Targeted by RDR6-Mediated RNA Silencing in ArabidopsisZhenghua Luo, Zhixiang ChenPlant Cell Mar 2007, 19 (3) 943-958; DOI: https://doi.org/10.1105/tpc.106.045724
RNA silencing induced by sense transgenes may protect against excessively expressed genes, but whether all transcripts or just aberrant ones activate genome surveillance is unclear. Constructs with enhanced or reduced production of aberrant RNAs were tested and showed RNA-DEPENDENT RNA POLYMERASE6–mediated silencing induced by aberrant mRNAs, which probably act as templates for the RNA polymerase.
Conti, Lucio
- You have accessRestricted AccessTERMINAL FLOWER1 Is a Mobile Signal Controlling Arabidopsis ArchitectureLucio Conti, Desmond BradleyPlant Cell Mar 2007, 19 (3) 767-778; DOI: https://doi.org/10.1105/tpc.106.049767
TERMINAL FLOWER1 (TFL1) affects the number and identity of aerial organs. TFL1 mRNA is restricted to the inner cells of the meristem, but this work reveals that the protein is a mobile signal that becomes evenly distributed across the meristem and suggests a model for how floral and shoot meristems establish a feedback loop of expression and protein movement to control inflorescence architecture.
Cumbie, Jason S.
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
D
Dall'Osto, Luca
- You have accessRestricted AccessThe Arabidopsis aba4-1 Mutant Reveals a Specific Function for Neoxanthin in Protection against Photooxidative StressLuca Dall'Osto, Stefano Cazzaniga, Helen North, Annie Marion-Poll, Roberto BassiPlant Cell Mar 2007, 19 (3) 1048-1064; DOI: https://doi.org/10.1105/tpc.106.049114
Carotenoids help protect the chloroplast from photoxidative stress. The aba4-1 mutant lacks neoxanthin but retains all other xanthophyll species. This research shows that neoxanthin plays a specific role in protection of Lhc proteins, the PSII reaction center, and thylakoids from photooxidative stress and protects against the damaging effects of reactive oxygen species, particularly superoxide anions.
Dell'Olivo, Alexandre
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
Deng, Xing Wang
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Deslandes, Laurent
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Dissmeyer, Nico
- Open AccessT-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate ResidueNico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini, Arp SchnittgerPlant Cell Mar 2007, 19 (3) 972-985; DOI: https://doi.org/10.1105/tpc.107.050401
To divide or not is one of the most crucial choices made by cells. As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases (CDKs), and one of the major regulatory inputs to CDK function is phosphorylation. Here, it is shown that Arabidopsis CDKA;1 is phosphorylated in the T-loop region and that phosphorylation is essential for kinase function.
Doke, Noriyuki
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
Dolan, Liam
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Duy, Daniela
- You have accessRestricted AccessPIC1, an Ancient Permease in Arabidopsis Chloroplasts, Mediates Iron TransportDaniela Duy, Gerhard Wanner, Anderson R. Meda, Nicolaus von Wirén, Jürgen Soll, Katrin PhilipparPlant Cell Mar 2007, 19 (3) 986-1006; DOI: https://doi.org/10.1105/tpc.106.047407
Chloroplasts are a major sink for metal ions like iron and copper, which play essential roles in photosynthetic electron transport. However, very little is known about iron uptake by the chloroplast. This study characterized PIC1 from Arabidopsis, which is a transmembrane protein of cyanobacterial origin that most likely functions as a metal ion permease in the chloroplast envelope.
E
Eckardt, Nancy A.
- You have accessRestricted AccessLight Regulation of Plant Development: HY5 Genomic Binding SitesNancy A. EckardtPlant Cell Mar 2007, 19 (3) 727-729; DOI: https://doi.org/10.1105/tpc.107.052233
- You have accessRestricted AccessFocus on Meiotic Crossover InterferenceNancy A. EckardtPlant Cell Mar 2007, 19 (3) 730a; DOI: https://doi.org/10.1105/tpc.107.190311
- You have accessRestricted AccessNovel Oxylipin Signaling CascadesNancy A. EckardtPlant Cell Mar 2007, 19 (3) 730; DOI: https://doi.org/10.1105/tpc.107.190310
Eyal, Yoram
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
F
Fahlgren, Noah
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
Feng, Dong Xin
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Figueroa, Pablo
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Franco-Zorrilla, José Manuel
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Fujiwara, Masayuki
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
G
Gal-On, Amit
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Gao, Ying
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
GarcÃa-Ponce, Berenice
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Gidoni, David
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Givan, Scott A.
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
Goldschmidt, Eliezer E.
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Gould, Billie
- You have accessRestricted AccessElaboration of B Gene Function to Include the Identity of Novel Floral Organs in the Lower Eudicot AquilegiaElena M. Kramer, Lynn Holappa, Billie Gould, M. Alejandra Jaramillo, Dimitriy Setnikov, Philip M. SantiagoPlant Cell Mar 2007, 19 (3) 750-766; DOI: https://doi.org/10.1105/tpc.107.050385
The classical ABC model postulates three functionally overlapping classes of gene activity to establish the identity of four organ types, but some plants, such as Aquilegia, exhibit variation in floral organ identity that suggests a more complex ABC program. Expression and yeast two-hybrid analyses as well as gene silencing reveal that canonical B class function has been elaborated in Aquilegia.
Grini, Paul E.
- Open AccessT-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate ResidueNico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini, Arp SchnittgerPlant Cell Mar 2007, 19 (3) 972-985; DOI: https://doi.org/10.1105/tpc.107.050401
To divide or not is one of the most crucial choices made by cells. As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases (CDKs), and one of the major regulatory inputs to CDK function is phosphorylation. Here, it is shown that Arabidopsis CDKA;1 is phosphorylated in the T-loop region and that phosphorylation is essential for kinase function.
Gübitz, Thomas
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
H
Hamberg, Mats
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Harpaz-Saad, Smadar
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
He, Guangcun
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
He, Kun
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
He, Ruifeng
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
He, Yuke
- You have accessRestricted AccessThe N-Terminal Double-Stranded RNA Binding Domains of Arabidopsis HYPONASTIC LEAVES1 Are Sufficient for Pre-MicroRNA ProcessingFeijie Wu, Lin Yu, Wenguang Cao, Yanfei Mao, Zhongyuan Liu, Yuke HePlant Cell Mar 2007, 19 (3) 914-925; DOI: https://doi.org/10.1105/tpc.106.048637
Some double-stranded RNA binding proteins participate in miRNA biogenesis. A series of deletion mutants demonstrated that the N-terminal region of HYPONASTIC LEAVES1 with two double-stranded RNA binding domains is sufficient for miRNA biogenesis and capable of rescuing the hyl1 phenotype. This study also showed that the putative NLS is a true nuclear localization signal.
Hernández-Blanco, Camilo
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Hettenhausen, Christian
- You have accessRestricted AccessHerbivory Rapidly Activates MAPK Signaling in Attacked and Unattacked Leaf Regions but Not between Leaves of Nicotiana attenuataJianqiang Wu, Christian Hettenhausen, Stefan Meldau, Ian T. BaldwinPlant Cell Mar 2007, 19 (3) 1096-1122; DOI: https://doi.org/10.1105/tpc.106.049353
Mitogen-activated protein kinase (MAPK) signaling mediates plant responses to herbivore attack, but the transduction of these signals is poorly understood. This work examines how plants deploy defense reactions and demonstrates that herbivore oral secretions (OS) dramatically amplify wound-induced MAPK activity and that fatty acid–amino acid conjugates in herbivore OS are the elicitors.
Heyting, Christa
- Open AccessThe Mismatch Repair Protein MLH1 Marks a Subset of Strongly Interfering Crossovers in TomatoFranck G.P. Lhuissier, Hildo H. Offenberg, Peter E. Wittich, Norbert O.E. Vischer, Christa HeytingPlant Cell Mar 2007, 19 (3) 862-876; DOI: https://doi.org/10.1105/tpc.106.049106
During meiosis, crossovers (COs) occur between homologous chromosomes, and CO positions are marked by protein complexes named late recombination nodules (LNs). This study shows that the mismatch repair protein MLH1 occurs in a specific subset of LNs and maps the chromosomal positions of the MLH1 foci. The maps of MLH1 foci and LNs could be valuable in various applications, including hybrid genetics.
Hoballah, Maria Elena
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
Holappa, Lynn
- You have accessRestricted AccessElaboration of B Gene Function to Include the Identity of Novel Floral Organs in the Lower Eudicot AquilegiaElena M. Kramer, Lynn Holappa, Billie Gould, M. Alejandra Jaramillo, Dimitriy Setnikov, Philip M. SantiagoPlant Cell Mar 2007, 19 (3) 750-766; DOI: https://doi.org/10.1105/tpc.107.050385
The classical ABC model postulates three functionally overlapping classes of gene activity to establish the identity of four organ types, but some plants, such as Aquilegia, exhibit variation in floral organ identity that suggests a more complex ABC program. Expression and yeast two-hybrid analyses as well as gene silencing reveal that canonical B class function has been elaborated in Aquilegia.
Hooker, Tanya S.
- You have accessRestricted AccessA Core Subunit of the RNA-Processing/Degrading Exosome Specifically Influences Cuticular Wax Biosynthesis in ArabidopsisTanya S. Hooker, Patricia Lam, Huanquan Zheng, Ljerka KunstPlant Cell Mar 2007, 19 (3) 904-913; DOI: https://doi.org/10.1105/tpc.106.049304
The cuticle, an extracellular matrix of cutin polyester and waxes, covers and protects plant aerial organs, but little is known about the regulation of cuticular wax deposition. Mutant analysis and gene isolation and characterization demonstrate that wax production is controlled, at least in part, by the mRNA stability of a repressor that is recognized and degraded by the CER7 ribonuclease.
Hörtensteiner, Stefan
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Howell, Miya D.
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
Hu, Jian
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
I
Ichimura, Kazuya
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Inzé, Dirk
- Open AccessT-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate ResidueNico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini, Arp SchnittgerPlant Cell Mar 2007, 19 (3) 972-985; DOI: https://doi.org/10.1105/tpc.107.050401
To divide or not is one of the most crucial choices made by cells. As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases (CDKs), and one of the major regulatory inputs to CDK function is phosphorylation. Here, it is shown that Arabidopsis CDKA;1 is phosphorylated in the T-loop region and that phosphorylation is essential for kinase function.
J
Jaramillo, M. Alejandra
- You have accessRestricted AccessElaboration of B Gene Function to Include the Identity of Novel Floral Organs in the Lower Eudicot AquilegiaElena M. Kramer, Lynn Holappa, Billie Gould, M. Alejandra Jaramillo, Dimitriy Setnikov, Philip M. SantiagoPlant Cell Mar 2007, 19 (3) 750-766; DOI: https://doi.org/10.1105/tpc.107.050385
The classical ABC model postulates three functionally overlapping classes of gene activity to establish the identity of four organ types, but some plants, such as Aquilegia, exhibit variation in floral organ identity that suggests a more complex ABC program. Expression and yeast two-hybrid analyses as well as gene silencing reveal that canonical B class function has been elaborated in Aquilegia.
Jin, Lifeng
- You have accessRestricted AccessArabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 Functions in Directional Cell Growth by Destabilizing Cortical MicrotubulesXia Wang, Lei Zhu, Baoquan Liu, Che Wang, Lifeng Jin, Qian Zhao, Ming YuanPlant Cell Mar 2007, 19 (3) 877-889; DOI: https://doi.org/10.1105/tpc.106.048579
Mutations in microtubule-associated proteins (MAPs) result in abnormal plant development and plant cell morphogenesis due to disruption of microtubule (MT) organization. This study identified Arabidopsis MAP18, which binds to microtubules and inhibits tubulin polymerization, destabilizes MTs, and plays an important role in the regulation of MT organization to determine directional plant cell growth.
K
Kapri-Pardes, Einat
- You have accessRestricted AccessThe Thylakoid Lumen Protease Deg1 Is Involved in the Repair of Photosystem II from Photoinhibition in ArabidopsisEinat Kapri-Pardes, Leah Naveh, Zach AdamPlant Cell Mar 2007, 19 (3) 1039-1047; DOI: https://doi.org/10.1105/tpc.106.046573
Photosynthesis is accompanied by accumulation of highly oxidizing species and oxygen radicals that cause photodamage within the photosystem II complex. Removal of damaged copies of the D1 protein is a prerequisite for reassembly of this complex. RNAi mutant and other analyses suggest that the lumenal protease Deg1 is involved in degradation of the D1 protein during PSII repair from photoinhibition.
Kasschau, Kristin D.
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
Kawakita, Kazuhito
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
Keller, Harald
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Kobayashi, Michie
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
Komine, Yutaka
- Open AccessIntegration of Chloroplast Nucleic Acid Metabolism into the Phosphate Deprivation Response in Chlamydomonas reinhardtiiShlomit Yehudai-Resheff, Sara L. Zimmer, Yutaka Komine, David B. SternPlant Cell Mar 2007, 19 (3) 1023-1038; DOI: https://doi.org/10.1105/tpc.106.045427
The role of the chloroplast as a phosphorus (P) reservoir in acclimation to P limitation is not well known. Polynucleotide phosphorylase consumes P through RNA decay, and while P deprivation usually stimulates RNA decay, in Chlamydomonas chloroplasts RNA levels rise but DNA levels fall during acclimation, suggesting that chloroplast polyploidy may be adaptive as a repository for P.
Kramer, Elena M.
- You have accessRestricted AccessElaboration of B Gene Function to Include the Identity of Novel Floral Organs in the Lower Eudicot AquilegiaElena M. Kramer, Lynn Holappa, Billie Gould, M. Alejandra Jaramillo, Dimitriy Setnikov, Philip M. SantiagoPlant Cell Mar 2007, 19 (3) 750-766; DOI: https://doi.org/10.1105/tpc.107.050385
The classical ABC model postulates three functionally overlapping classes of gene activity to establish the identity of four organ types, but some plants, such as Aquilegia, exhibit variation in floral organ identity that suggests a more complex ABC program. Expression and yeast two-hybrid analyses as well as gene silencing reveal that canonical B class function has been elaborated in Aquilegia.
Kuhlemeier, Cris
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
Kühn, Kristina
- You have accessRestricted AccessArabidopsis Phage-Type RNA Polymerases: Accurate in Vitro Transcription of Organellar GenesKristina Kühn, Alexandra-Viola Bohne, Karsten Liere, Andreas Weihe, Thomas BörnerPlant Cell Mar 2007, 19 (3) 959-971; DOI: https://doi.org/10.1105/tpc.106.046839
Three Arabidopsis nuclear-encoded T7 phage-type RNA polymerases (RNAPs) localize to mitochondria, plastids, or both. This study finds that two of them can initiate transcription from both mitochondrial and plastid promoters in vitro without auxiliary factors. The ability for promoter recognition appears to have been conserved over a long period of evolution of organellar RNAPs.
Kunst, Ljerka
- You have accessRestricted AccessA Core Subunit of the RNA-Processing/Degrading Exosome Specifically Influences Cuticular Wax Biosynthesis in ArabidopsisTanya S. Hooker, Patricia Lam, Huanquan Zheng, Ljerka KunstPlant Cell Mar 2007, 19 (3) 904-913; DOI: https://doi.org/10.1105/tpc.106.049304
The cuticle, an extracellular matrix of cutin polyester and waxes, covers and protects plant aerial organs, but little is known about the regulation of cuticular wax deposition. Mutant analysis and gene isolation and characterization demonstrate that wax production is controlled, at least in part, by the mRNA stability of a repressor that is recognized and degraded by the CER7 ribonuclease.
L
Lam, Patricia
- You have accessRestricted AccessA Core Subunit of the RNA-Processing/Degrading Exosome Specifically Influences Cuticular Wax Biosynthesis in ArabidopsisTanya S. Hooker, Patricia Lam, Huanquan Zheng, Ljerka KunstPlant Cell Mar 2007, 19 (3) 904-913; DOI: https://doi.org/10.1105/tpc.106.049304
The cuticle, an extracellular matrix of cutin polyester and waxes, covers and protects plant aerial organs, but little is known about the regulation of cuticular wax deposition. Mutant analysis and gene isolation and characterization demonstrate that wax production is controlled, at least in part, by the mRNA stability of a repressor that is recognized and degraded by the CER7 ribonuclease.
Lanza, Mónica
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Lee, Horim
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Lee, Ilha
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Lee, Jungeun
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Lemtiri-Chlieh, Fouad
- You have accessRestricted AccessDeath Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate ImmunityRashid Ali, Wei Ma, Fouad Lemtiri-Chlieh, Dimitrios Tsaltas, Qiang Leng, Susannne von Bodman, Gerald A. BerkowitzPlant Cell Mar 2007, 19 (3) 1081-1095; DOI: https://doi.org/10.1105/tpc.106.045096
Plants lacking a Ca2+-conducting channel do not undergo hypersensitive response to pathogens and have impaired nitric oxide generation in response to application of a pathogen-associated molecular pattern elicitor. Patch clamp analysis demonstrated elicitor activation of inward cation currents; this work provides insights regarding early steps in pathogen response signaling in plants.
Leng, Qiang
- You have accessRestricted AccessDeath Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate ImmunityRashid Ali, Wei Ma, Fouad Lemtiri-Chlieh, Dimitrios Tsaltas, Qiang Leng, Susannne von Bodman, Gerald A. BerkowitzPlant Cell Mar 2007, 19 (3) 1081-1095; DOI: https://doi.org/10.1105/tpc.106.045096
Plants lacking a Ca2+-conducting channel do not undergo hypersensitive response to pathogens and have impaired nitric oxide generation in response to application of a pathogen-associated molecular pattern elicitor. Patch clamp analysis demonstrated elicitor activation of inward cation currents; this work provides insights regarding early steps in pathogen response signaling in plants.
Leyva, Antonio
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Lhuissier, Franck G.P.
- Open AccessThe Mismatch Repair Protein MLH1 Marks a Subset of Strongly Interfering Crossovers in TomatoFranck G.P. Lhuissier, Hildo H. Offenberg, Peter E. Wittich, Norbert O.E. Vischer, Christa HeytingPlant Cell Mar 2007, 19 (3) 862-876; DOI: https://doi.org/10.1105/tpc.106.049106
During meiosis, crossovers (COs) occur between homologous chromosomes, and CO positions are marked by protein complexes named late recombination nodules (LNs). This study shows that the mismatch repair protein MLH1 occurs in a specific subset of LNs and maps the chromosomal positions of the MLH1 foci. The maps of MLH1 foci and LNs could be valuable in various applications, including hybrid genetics.
Liere, Karsten
- You have accessRestricted AccessArabidopsis Phage-Type RNA Polymerases: Accurate in Vitro Transcription of Organellar GenesKristina Kühn, Alexandra-Viola Bohne, Karsten Liere, Andreas Weihe, Thomas BörnerPlant Cell Mar 2007, 19 (3) 959-971; DOI: https://doi.org/10.1105/tpc.106.046839
Three Arabidopsis nuclear-encoded T7 phage-type RNA polymerases (RNAPs) localize to mitochondria, plastids, or both. This study finds that two of them can initiate transcription from both mitochondrial and plastid promoters in vitro without auxiliary factors. The ability for promoter recognition appears to have been conserved over a long period of evolution of organellar RNAPs.
Liu, Baoquan
- You have accessRestricted AccessArabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 Functions in Directional Cell Growth by Destabilizing Cortical MicrotubulesXia Wang, Lei Zhu, Baoquan Liu, Che Wang, Lifeng Jin, Qian Zhao, Ming YuanPlant Cell Mar 2007, 19 (3) 877-889; DOI: https://doi.org/10.1105/tpc.106.048579
Mutations in microtubule-associated proteins (MAPs) result in abnormal plant development and plant cell morphogenesis due to disruption of microtubule (MT) organization. This study identified Arabidopsis MAP18, which binds to microtubules and inhibits tubulin polymerization, destabilizes MTs, and plays an important role in the regulation of MT organization to determine directional plant cell growth.
Liu, Zhongyuan
- You have accessRestricted AccessThe N-Terminal Double-Stranded RNA Binding Domains of Arabidopsis HYPONASTIC LEAVES1 Are Sufficient for Pre-MicroRNA ProcessingFeijie Wu, Lin Yu, Wenguang Cao, Yanfei Mao, Zhongyuan Liu, Yuke HePlant Cell Mar 2007, 19 (3) 914-925; DOI: https://doi.org/10.1105/tpc.106.048637
Some double-stranded RNA binding proteins participate in miRNA biogenesis. A series of deletion mutants demonstrated that the N-terminal region of HYPONASTIC LEAVES1 with two double-stranded RNA binding domains is sufficient for miRNA biogenesis and capable of rescuing the hyl1 phenotype. This study also showed that the putative NLS is a true nuclear localization signal.
Llorente, Francisco
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
López, Miguel Angel
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Luo, Zhenghua
- You have accessRestricted AccessImproperly Terminated, Unpolyadenylated mRNA of Sense Transgenes Is Targeted by RDR6-Mediated RNA Silencing in ArabidopsisZhenghua Luo, Zhixiang ChenPlant Cell Mar 2007, 19 (3) 943-958; DOI: https://doi.org/10.1105/tpc.106.045724
RNA silencing induced by sense transgenes may protect against excessively expressed genes, but whether all transcripts or just aberrant ones activate genome surveillance is unclear. Constructs with enhanced or reduced production of aberrant RNAs were tested and showed RNA-DEPENDENT RNA POLYMERASE6–mediated silencing induced by aberrant mRNAs, which probably act as templates for the RNA polymerase.
M
Ma, Wei
- You have accessRestricted AccessDeath Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate ImmunityRashid Ali, Wei Ma, Fouad Lemtiri-Chlieh, Dimitrios Tsaltas, Qiang Leng, Susannne von Bodman, Gerald A. BerkowitzPlant Cell Mar 2007, 19 (3) 1081-1095; DOI: https://doi.org/10.1105/tpc.106.045096
Plants lacking a Ca2+-conducting channel do not undergo hypersensitive response to pathogens and have impaired nitric oxide generation in response to application of a pathogen-associated molecular pattern elicitor. Patch clamp analysis demonstrated elicitor activation of inward cation currents; this work provides insights regarding early steps in pathogen response signaling in plants.
Mandel, Therese
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
Mao, Yanfei
- You have accessRestricted AccessThe N-Terminal Double-Stranded RNA Binding Domains of Arabidopsis HYPONASTIC LEAVES1 Are Sufficient for Pre-MicroRNA ProcessingFeijie Wu, Lin Yu, Wenguang Cao, Yanfei Mao, Zhongyuan Liu, Yuke HePlant Cell Mar 2007, 19 (3) 914-925; DOI: https://doi.org/10.1105/tpc.106.048637
Some double-stranded RNA binding proteins participate in miRNA biogenesis. A series of deletion mutants demonstrated that the N-terminal region of HYPONASTIC LEAVES1 with two double-stranded RNA binding domains is sufficient for miRNA biogenesis and capable of rescuing the hyl1 phenotype. This study also showed that the putative NLS is a true nuclear localization signal.
Marco, Yves
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Marion-Poll, Annie
- You have accessRestricted AccessThe Arabidopsis aba4-1 Mutant Reveals a Specific Function for Neoxanthin in Protection against Photooxidative StressLuca Dall'Osto, Stefano Cazzaniga, Helen North, Annie Marion-Poll, Roberto BassiPlant Cell Mar 2007, 19 (3) 1048-1064; DOI: https://doi.org/10.1105/tpc.106.049114
Carotenoids help protect the chloroplast from photoxidative stress. The aba4-1 mutant lacks neoxanthin but retains all other xanthophyll species. This research shows that neoxanthin plays a specific role in protection of Lhc proteins, the PSII reaction center, and thylakoids from photooxidative stress and protects against the damaging effects of reactive oxygen species, particularly superoxide anions.
MartÃnez, Marta
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Maruyama, Kyonoshin
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Meda, Anderson R.
- You have accessRestricted AccessPIC1, an Ancient Permease in Arabidopsis Chloroplasts, Mediates Iron TransportDaniela Duy, Gerhard Wanner, Anderson R. Meda, Nicolaus von Wirén, Jürgen Soll, Katrin PhilipparPlant Cell Mar 2007, 19 (3) 986-1006; DOI: https://doi.org/10.1105/tpc.106.047407
Chloroplasts are a major sink for metal ions like iron and copper, which play essential roles in photosynthetic electron transport. However, very little is known about iron uptake by the chloroplast. This study characterized PIC1 from Arabidopsis, which is a transmembrane protein of cyanobacterial origin that most likely functions as a metal ion permease in the chloroplast envelope.
Meldau, Stefan
- You have accessRestricted AccessHerbivory Rapidly Activates MAPK Signaling in Attacked and Unattacked Leaf Regions but Not between Leaves of Nicotiana attenuataJianqiang Wu, Christian Hettenhausen, Stefan Meldau, Ian T. BaldwinPlant Cell Mar 2007, 19 (3) 1096-1122; DOI: https://doi.org/10.1105/tpc.106.049353
Mitogen-activated protein kinase (MAPK) signaling mediates plant responses to herbivore attack, but the transduction of these signals is poorly understood. This work examines how plants deploy defense reactions and demonstrates that herbivore oral secretions (OS) dramatically amplify wound-induced MAPK activity and that fatty acid–amino acid conjugates in herbivore OS are the elicitors.
Mett, Anahit
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Miao, Ying
- You have accessRestricted AccessThe Antagonist Function of Arabidopsis WRKY53 and ESR/ESP in Leaf Senescence Is Modulated by the Jasmonic and Salicylic Acid EquilibriumYing Miao, Ulrike ZentgrafPlant Cell Mar 2007, 19 (3) 819-830; DOI: https://doi.org/10.1105/tpc.106.042705
Crosstalk between salicylic acid (SA) and jasmonic acid (JA) in leaf senescence is not well-known. This work shows that the senescence-specific transcription factor WRKY53 is positively regulated by SA and negatively by JA signaling and that the antagonistically regulated EPITHIOSPECIFYING SENESCENCE REGULATOR functions in the cytoplasm in nitrile biosynthesis but in the nucleus as an interaction partner with WRKY53.
Mizoguchi, Tsuyoshi
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Molina, Antonio
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
N
Naveh, Leah
- You have accessRestricted AccessThe Thylakoid Lumen Protease Deg1 Is Involved in the Repair of Photosystem II from Photoinhibition in ArabidopsisEinat Kapri-Pardes, Leah Naveh, Zach AdamPlant Cell Mar 2007, 19 (3) 1039-1047; DOI: https://doi.org/10.1105/tpc.106.046573
Photosynthesis is accompanied by accumulation of highly oxidizing species and oxygen radicals that cause photodamage within the photosystem II complex. Removal of damaged copies of the D1 protein is a prerequisite for reassembly of this complex. RNAi mutant and other analyses suggest that the lumenal protease Deg1 is involved in degradation of the D1 protein during PSII repair from photoinhibition.
North, Helen
- You have accessRestricted AccessThe Arabidopsis aba4-1 Mutant Reveals a Specific Function for Neoxanthin in Protection against Photooxidative StressLuca Dall'Osto, Stefano Cazzaniga, Helen North, Annie Marion-Poll, Roberto BassiPlant Cell Mar 2007, 19 (3) 1048-1064; DOI: https://doi.org/10.1105/tpc.106.049114
Carotenoids help protect the chloroplast from photoxidative stress. The aba4-1 mutant lacks neoxanthin but retains all other xanthophyll species. This research shows that neoxanthin plays a specific role in protection of Lhc proteins, the PSII reaction center, and thylakoids from photooxidative stress and protects against the damaging effects of reactive oxygen species, particularly superoxide anions.
Nowack, Moritz K.
- Open AccessT-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate ResidueNico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini, Arp SchnittgerPlant Cell Mar 2007, 19 (3) 972-985; DOI: https://doi.org/10.1105/tpc.107.050401
To divide or not is one of the most crucial choices made by cells. As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases (CDKs), and one of the major regulatory inputs to CDK function is phosphorylation. Here, it is shown that Arabidopsis CDKA;1 is phosphorylated in the T-loop region and that phosphorylation is essential for kinase function.
O
Offenberg, Hildo H.
- Open AccessThe Mismatch Repair Protein MLH1 Marks a Subset of Strongly Interfering Crossovers in TomatoFranck G.P. Lhuissier, Hildo H. Offenberg, Peter E. Wittich, Norbert O.E. Vischer, Christa HeytingPlant Cell Mar 2007, 19 (3) 862-876; DOI: https://doi.org/10.1105/tpc.106.049106
During meiosis, crossovers (COs) occur between homologous chromosomes, and CO positions are marked by protein complexes named late recombination nodules (LNs). This study shows that the mismatch repair protein MLH1 occurs in a specific subset of LNs and maps the chromosomal positions of the MLH1 foci. The maps of MLH1 foci and LNs could be valuable in various applications, including hybrid genetics.
Ohura, Ikuko
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
P
Paz-Ares, Javier
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Philippar, Katrin
- You have accessRestricted AccessPIC1, an Ancient Permease in Arabidopsis Chloroplasts, Mediates Iron TransportDaniela Duy, Gerhard Wanner, Anderson R. Meda, Nicolaus von Wirén, Jürgen Soll, Katrin PhilipparPlant Cell Mar 2007, 19 (3) 986-1006; DOI: https://doi.org/10.1105/tpc.106.047407
Chloroplasts are a major sink for metal ions like iron and copper, which play essential roles in photosynthetic electron transport. However, very little is known about iron uptake by the chloroplast. This study characterized PIC1 from Arabidopsis, which is a transmembrane protein of cyanobacterial origin that most likely functions as a metal ion permease in the chloroplast envelope.
Pusch, Stefan
- Open AccessT-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate ResidueNico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini, Arp SchnittgerPlant Cell Mar 2007, 19 (3) 972-985; DOI: https://doi.org/10.1105/tpc.107.050401
To divide or not is one of the most crucial choices made by cells. As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases (CDKs), and one of the major regulatory inputs to CDK function is phosphorylation. Here, it is shown that Arabidopsis CDKA;1 is phosphorylated in the T-loop region and that phosphorylation is essential for kinase function.
S
Sánchez-RodrÃguez, Clara
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Sánchez-Vallet, Andrea
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Santiago, Philip M.
- You have accessRestricted AccessElaboration of B Gene Function to Include the Identity of Novel Floral Organs in the Lower Eudicot AquilegiaElena M. Kramer, Lynn Holappa, Billie Gould, M. Alejandra Jaramillo, Dimitriy Setnikov, Philip M. SantiagoPlant Cell Mar 2007, 19 (3) 750-766; DOI: https://doi.org/10.1105/tpc.107.050385
The classical ABC model postulates three functionally overlapping classes of gene activity to establish the identity of four organ types, but some plants, such as Aquilegia, exhibit variation in floral organ identity that suggests a more complex ABC program. Expression and yeast two-hybrid analyses as well as gene silencing reveal that canonical B class function has been elaborated in Aquilegia.
Schnittger, Arp
- Open AccessT-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate ResidueNico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini, Arp SchnittgerPlant Cell Mar 2007, 19 (3) 972-985; DOI: https://doi.org/10.1105/tpc.107.050401
To divide or not is one of the most crucial choices made by cells. As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases (CDKs), and one of the major regulatory inputs to CDK function is phosphorylation. Here, it is shown that Arabidopsis CDKA;1 is phosphorylated in the T-loop region and that phosphorylation is essential for kinase function.
Segura, Ma Dolores
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Seo, Shigemi
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Setnikov, Dimitriy
- You have accessRestricted AccessElaboration of B Gene Function to Include the Identity of Novel Floral Organs in the Lower Eudicot AquilegiaElena M. Kramer, Lynn Holappa, Billie Gould, M. Alejandra Jaramillo, Dimitriy Setnikov, Philip M. SantiagoPlant Cell Mar 2007, 19 (3) 750-766; DOI: https://doi.org/10.1105/tpc.107.050385
The classical ABC model postulates three functionally overlapping classes of gene activity to establish the identity of four organ types, but some plants, such as Aquilegia, exhibit variation in floral organ identity that suggests a more complex ABC program. Expression and yeast two-hybrid analyses as well as gene silencing reveal that canonical B class function has been elaborated in Aquilegia.
Shao, Zhe
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
Shiboleth, Yoel M.
- You have accessRestricted AccessChlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally RegulatedSmadar Harpaz-Saad, Tamar Azoulay, Tzahi Arazi, Eran Ben-Yaakov, Anahit Mett, Yoel M. Shiboleth, Stefan Hörtensteiner, David Gidoni, Amit Gal-On, Eliezer E. Goldschmidt, Yoram EyalPlant Cell Mar 2007, 19 (3) 1007-1022; DOI: https://doi.org/10.1105/tpc.107.050633
Chlorophyll is a central player in photosynthetic light harvesting, but details of chlorophyll catabolism and regulation are unresolved. Here, two different expression systems in two species were used to study the role and regulation of Chlase in chlorophyll catabolism. The results suggest that Chlase is a rate-limiting enzyme in chlorophyll catabolism controlled via posttranslational regulation.
Shimamoto, Ko
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
Shinozaki, Kazuo
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Solano, Roberto
- You have accessRestricted AccessA Mutant of the Arabidopsis Phosphate Transporter PHT1;1 Displays Enhanced Arsenic AccumulationPablo Catarecha, Ma Dolores Segura, José Manuel Franco-Zorrilla, Berenice GarcÃa-Ponce, Mónica Lanza, Roberto Solano, Javier Paz-Ares, Antonio LeyvaPlant Cell Mar 2007, 19 (3) 1123-1133; DOI: https://doi.org/10.1105/tpc.106.041871
Arsenate [As(V)], the most abundant chemical form of the toxic element arsenic, is very similar to phosphate (Pi), which enables its uptake from the soil through the high-affinity Pi transport system. This work identifies a Pi transporter mutant with enhanced ability to accumulate arsenic but with a reduced Pi and As(V) uptake rate, suggesting a protective mechanism operating in arsenic-tolerant plants.
Soll, Jürgen
- You have accessRestricted AccessPIC1, an Ancient Permease in Arabidopsis Chloroplasts, Mediates Iron TransportDaniela Duy, Gerhard Wanner, Anderson R. Meda, Nicolaus von Wirén, Jürgen Soll, Katrin PhilipparPlant Cell Mar 2007, 19 (3) 986-1006; DOI: https://doi.org/10.1105/tpc.106.047407
Chloroplasts are a major sink for metal ions like iron and copper, which play essential roles in photosynthetic electron transport. However, very little is known about iron uptake by the chloroplast. This study characterized PIC1 from Arabidopsis, which is a transmembrane protein of cyanobacterial origin that most likely functions as a metal ion permease in the chloroplast envelope.
Somerville, Shauna
- You have accessRestricted AccessImpairment of Cellulose Synthases Required for Arabidopsis Secondary Cell Wall Formation Enhances Disease ResistanceCamilo Hernández-Blanco, Dong Xin Feng, Jian Hu, Andrea Sánchez-Vallet, Laurent Deslandes, Francisco Llorente, Marta Berrocal-Lobo, Harald Keller, Xavier Barlet, Clara Sánchez-RodrÃguez, Lisa K. Anderson, Shauna Somerville, Yves Marco, Antonio MolinaPlant Cell Mar 2007, 19 (3) 890-903; DOI: https://doi.org/10.1105/tpc.106.048058
Mutations in cellulose synthase subunits required for secondary cell wall formation confer resistance to bacterial and fungal pathogens and upregulate ABA-responsive defense genes. Alteration of secondary cell wall integrity by inhibiting cellulose synthesis may lead to activation of novel defense pathways that create antimicrobial-enriched environments hostile to pathogens.
Stals, Hilde
- Open AccessT-Loop Phosphorylation of Arabidopsis CDKA;1 Is Required for Its Function and Can Be Partially Substituted by an Aspartate ResidueNico Dissmeyer, Moritz K. Nowack, Stefan Pusch, Hilde Stals, Dirk Inzé, Paul E. Grini, Arp SchnittgerPlant Cell Mar 2007, 19 (3) 972-985; DOI: https://doi.org/10.1105/tpc.107.050401
To divide or not is one of the most crucial choices made by cells. As in other eukaryotes, progression through the cell cycle in plants is governed by cyclin-dependent kinases (CDKs), and one of the major regulatory inputs to CDK function is phosphorylation. Here, it is shown that Arabidopsis CDKA;1 is phosphorylated in the T-loop region and that phosphorylation is essential for kinase function.
Steber, Camille M.
- You have accessRestricted AccessSeed Germination of GA-Insensitive sleepy1 Mutants Does Not Require RGL2 Protein Disappearance in ArabidopsisTohru Ariizumi, Camille M. SteberPlant Cell Mar 2007, 19 (3) 791-804; DOI: https://doi.org/10.1105/tpc.106.048009
Proper regulation of seed dormancy and germination is critical to plant survival. It was thought that disappearance of the negative regulator RGA-LIKE2 (RGL2) protein is required for seed germination. This report finds instead that RGL2 accumulates in germinating sleepy1 (sly1) mutants, suggesting that protein disappearance is not the only mechanism that relieves RGL2 repression of seed germination.
Stern, David B.
- Open AccessIntegration of Chloroplast Nucleic Acid Metabolism into the Phosphate Deprivation Response in Chlamydomonas reinhardtiiShlomit Yehudai-Resheff, Sara L. Zimmer, Yutaka Komine, David B. SternPlant Cell Mar 2007, 19 (3) 1023-1038; DOI: https://doi.org/10.1105/tpc.106.045427
The role of the chloroplast as a phosphorus (P) reservoir in acclimation to P limitation is not well known. Polynucleotide phosphorylase consumes P through RNA decay, and while P deprivation usually stimulates RNA decay, in Chlamydomonas chloroplasts RNA levels rise but DNA levels fall during acclimation, suggesting that chloroplast polyploidy may be adaptive as a repository for P.
Stolc, Viktor
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Stuurman, Jeroen
- You have accessRestricted AccessSingle Gene–Mediated Shift in Pollinator Attraction in PetuniaMaria Elena Hoballah, Thomas Gübitz, Jeroen Stuurman, Larissa Broger, Mario Barone, Therese Mandel, Alexandre Dell'Olivo, Maeva Arnold, Cris KuhlemeierPlant Cell Mar 2007, 19 (3) 779-790; DOI: https://doi.org/10.1105/tpc.106.048694
Many floral traits appear to be adapted to particular pollinators. AN2 is a well-defined myb-type transcription factor that is a major determinant of flower color variation between two Petunia species. Transformation of one species with AN2 from the other caused a switch in pollinator preference, suggesting that adaptation to a new pollinator may involve a limited number of genes of large effect.
Sullivan, Christopher M.
- Open AccessGenome-Wide Analysis of the RNA-DEPENDENT RNA POLYMERASE6/DICER-LIKE4 Pathway in Arabidopsis Reveals Dependency on miRNA- and tasiRNA-Directed TargetingMiya D. Howell, Noah Fahlgren, Elisabeth J. Chapman, Jason S. Cumbie, Christopher M. Sullivan, Scott A. Givan, Kristin D. Kasschau, James C. CarringtonPlant Cell Mar 2007, 19 (3) 926-942; DOI: https://doi.org/10.1105/tpc.107.050062
Using high-throughput sequencing methods, many transcripts were found to be routed through the RDR6/DCL4-dependent silencing pathway after single or dual targeting events by miRNA or tasiRNA. Several members of a rapidly expanding clade of PPR gene transcripts were targeted, suggesting that this silencing pathway may function to buffer the effects of rapidly expanding gene families.
Sun, Mengxiang
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
T
Takahashi, Fuminori
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Tongprasit, Waraporn
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Tsaltas, Dimitrios
- You have accessRestricted AccessDeath Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate ImmunityRashid Ali, Wei Ma, Fouad Lemtiri-Chlieh, Dimitrios Tsaltas, Qiang Leng, Susannne von Bodman, Gerald A. BerkowitzPlant Cell Mar 2007, 19 (3) 1081-1095; DOI: https://doi.org/10.1105/tpc.106.045096
Plants lacking a Ca2+-conducting channel do not undergo hypersensitive response to pathogens and have impaired nitric oxide generation in response to application of a pathogen-associated molecular pattern elicitor. Patch clamp analysis demonstrated elicitor activation of inward cation currents; this work provides insights regarding early steps in pathogen response signaling in plants.
V
Vellosillo, Tamara
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Vicente, Jorge
- You have accessRestricted AccessOxylipins Produced by the 9-Lipoxygenase Pathway in Arabidopsis Regulate Lateral Root Development and Defense Responses through a Specific Signaling CascadeTamara Vellosillo, Marta MartÃnez, Miguel Angel López, Jorge Vicente, Tomas Cascón, Liam Dolan, Mats Hamberg, Carmen CastresanaPlant Cell Mar 2007, 19 (3) 831-846; DOI: https://doi.org/10.1105/tpc.106.046052
The importance of oxylipins, such as jasmonic acid, in plant defense and adaptation is well known. This work suggests that other oxylipins, such as 9-hydroxyoctadecatrienoic acid, also function in developmental and defense responses, such as callose and pectin deposition and production of reactive oxygen species. Mutant analyses revealed at least three signaling cascades mediating oxylipin action.
Vischer, Norbert O.E.
- Open AccessThe Mismatch Repair Protein MLH1 Marks a Subset of Strongly Interfering Crossovers in TomatoFranck G.P. Lhuissier, Hildo H. Offenberg, Peter E. Wittich, Norbert O.E. Vischer, Christa HeytingPlant Cell Mar 2007, 19 (3) 862-876; DOI: https://doi.org/10.1105/tpc.106.049106
During meiosis, crossovers (COs) occur between homologous chromosomes, and CO positions are marked by protein complexes named late recombination nodules (LNs). This study shows that the mismatch repair protein MLH1 occurs in a specific subset of LNs and maps the chromosomal positions of the MLH1 foci. The maps of MLH1 foci and LNs could be valuable in various applications, including hybrid genetics.
von Bodman, Susannne
- You have accessRestricted AccessDeath Don't Have No Mercy and Neither Does Calcium: Arabidopsis CYCLIC NUCLEOTIDE GATED CHANNEL2 and Innate ImmunityRashid Ali, Wei Ma, Fouad Lemtiri-Chlieh, Dimitrios Tsaltas, Qiang Leng, Susannne von Bodman, Gerald A. BerkowitzPlant Cell Mar 2007, 19 (3) 1081-1095; DOI: https://doi.org/10.1105/tpc.106.045096
Plants lacking a Ca2+-conducting channel do not undergo hypersensitive response to pathogens and have impaired nitric oxide generation in response to application of a pathogen-associated molecular pattern elicitor. Patch clamp analysis demonstrated elicitor activation of inward cation currents; this work provides insights regarding early steps in pathogen response signaling in plants.
von Wirén, Nicolaus
- You have accessRestricted AccessPIC1, an Ancient Permease in Arabidopsis Chloroplasts, Mediates Iron TransportDaniela Duy, Gerhard Wanner, Anderson R. Meda, Nicolaus von Wirén, Jürgen Soll, Katrin PhilipparPlant Cell Mar 2007, 19 (3) 986-1006; DOI: https://doi.org/10.1105/tpc.106.047407
Chloroplasts are a major sink for metal ions like iron and copper, which play essential roles in photosynthetic electron transport. However, very little is known about iron uptake by the chloroplast. This study characterized PIC1 from Arabidopsis, which is a transmembrane protein of cyanobacterial origin that most likely functions as a metal ion permease in the chloroplast envelope.
W
Wang, Che
- You have accessRestricted AccessArabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 Functions in Directional Cell Growth by Destabilizing Cortical MicrotubulesXia Wang, Lei Zhu, Baoquan Liu, Che Wang, Lifeng Jin, Qian Zhao, Ming YuanPlant Cell Mar 2007, 19 (3) 877-889; DOI: https://doi.org/10.1105/tpc.106.048579
Mutations in microtubule-associated proteins (MAPs) result in abnormal plant development and plant cell morphogenesis due to disruption of microtubule (MT) organization. This study identified Arabidopsis MAP18, which binds to microtubules and inhibits tubulin polymerization, destabilizes MTs, and plays an important role in the regulation of MT organization to determine directional plant cell growth.
Wang, Xia
- You have accessRestricted AccessArabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 Functions in Directional Cell Growth by Destabilizing Cortical MicrotubulesXia Wang, Lei Zhu, Baoquan Liu, Che Wang, Lifeng Jin, Qian Zhao, Ming YuanPlant Cell Mar 2007, 19 (3) 877-889; DOI: https://doi.org/10.1105/tpc.106.048579
Mutations in microtubule-associated proteins (MAPs) result in abnormal plant development and plant cell morphogenesis due to disruption of microtubule (MT) organization. This study identified Arabidopsis MAP18, which binds to microtubules and inhibits tubulin polymerization, destabilizes MTs, and plays an important role in the regulation of MT organization to determine directional plant cell growth.
Wang, Yuanyuan
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
Wanner, Gerhard
- You have accessRestricted AccessPIC1, an Ancient Permease in Arabidopsis Chloroplasts, Mediates Iron TransportDaniela Duy, Gerhard Wanner, Anderson R. Meda, Nicolaus von Wirén, Jürgen Soll, Katrin PhilipparPlant Cell Mar 2007, 19 (3) 986-1006; DOI: https://doi.org/10.1105/tpc.106.047407
Chloroplasts are a major sink for metal ions like iron and copper, which play essential roles in photosynthetic electron transport. However, very little is known about iron uptake by the chloroplast. This study characterized PIC1 from Arabidopsis, which is a transmembrane protein of cyanobacterial origin that most likely functions as a metal ion permease in the chloroplast envelope.
Wei, Zhe
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
Weihe, Andreas
- You have accessRestricted AccessArabidopsis Phage-Type RNA Polymerases: Accurate in Vitro Transcription of Organellar GenesKristina Kühn, Alexandra-Viola Bohne, Karsten Liere, Andreas Weihe, Thomas BörnerPlant Cell Mar 2007, 19 (3) 959-971; DOI: https://doi.org/10.1105/tpc.106.046839
Three Arabidopsis nuclear-encoded T7 phage-type RNA polymerases (RNAPs) localize to mitochondria, plastids, or both. This study finds that two of them can initiate transcription from both mitochondrial and plastid promoters in vitro without auxiliary factors. The ability for promoter recognition appears to have been conserved over a long period of evolution of organellar RNAPs.
Wittich, Peter E.
- Open AccessThe Mismatch Repair Protein MLH1 Marks a Subset of Strongly Interfering Crossovers in TomatoFranck G.P. Lhuissier, Hildo H. Offenberg, Peter E. Wittich, Norbert O.E. Vischer, Christa HeytingPlant Cell Mar 2007, 19 (3) 862-876; DOI: https://doi.org/10.1105/tpc.106.049106
During meiosis, crossovers (COs) occur between homologous chromosomes, and CO positions are marked by protein complexes named late recombination nodules (LNs). This study shows that the mismatch repair protein MLH1 occurs in a specific subset of LNs and maps the chromosomal positions of the MLH1 foci. The maps of MLH1 foci and LNs could be valuable in various applications, including hybrid genetics.
Wu, Feijie
- You have accessRestricted AccessThe N-Terminal Double-Stranded RNA Binding Domains of Arabidopsis HYPONASTIC LEAVES1 Are Sufficient for Pre-MicroRNA ProcessingFeijie Wu, Lin Yu, Wenguang Cao, Yanfei Mao, Zhongyuan Liu, Yuke HePlant Cell Mar 2007, 19 (3) 914-925; DOI: https://doi.org/10.1105/tpc.106.048637
Some double-stranded RNA binding proteins participate in miRNA biogenesis. A series of deletion mutants demonstrated that the N-terminal region of HYPONASTIC LEAVES1 with two double-stranded RNA binding domains is sufficient for miRNA biogenesis and capable of rescuing the hyl1 phenotype. This study also showed that the putative NLS is a true nuclear localization signal.
Wu, Jianqiang
- You have accessRestricted AccessHerbivory Rapidly Activates MAPK Signaling in Attacked and Unattacked Leaf Regions but Not between Leaves of Nicotiana attenuataJianqiang Wu, Christian Hettenhausen, Stefan Meldau, Ian T. BaldwinPlant Cell Mar 2007, 19 (3) 1096-1122; DOI: https://doi.org/10.1105/tpc.106.049353
Mitogen-activated protein kinase (MAPK) signaling mediates plant responses to herbivore attack, but the transduction of these signals is poorly understood. This work examines how plants deploy defense reactions and demonstrates that herbivore oral secretions (OS) dramatically amplify wound-induced MAPK activity and that fatty acid–amino acid conjugates in herbivore OS are the elicitors.
Y
Yamaguchi-Shinozaki, Kazuko
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Yehudai-Resheff, Shlomit
- Open AccessIntegration of Chloroplast Nucleic Acid Metabolism into the Phosphate Deprivation Response in Chlamydomonas reinhardtiiShlomit Yehudai-Resheff, Sara L. Zimmer, Yutaka Komine, David B. SternPlant Cell Mar 2007, 19 (3) 1023-1038; DOI: https://doi.org/10.1105/tpc.106.045427
The role of the chloroplast as a phosphorus (P) reservoir in acclimation to P limitation is not well known. Polynucleotide phosphorylase consumes P through RNA decay, and while P deprivation usually stimulates RNA decay, in Chlamydomonas chloroplasts RNA levels rise but DNA levels fall during acclimation, suggesting that chloroplast polyploidy may be adaptive as a repository for P.
Yokota, Naohiko
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
Yonezawa, Masahiro
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Yoshida, Riichiro
- Open AccessThe Mitogen-Activated Protein Kinase Cascade MKK3–MPK6 Is an Important Part of the Jasmonate Signal Transduction Pathway in ArabidopsisFuminori Takahashi, Riichiro Yoshida, Kazuya Ichimura, Tsuyoshi Mizoguchi, Shigemi Seo, Masahiro Yonezawa, Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, Kazuo ShinozakiPlant Cell Mar 2007, 19 (3) 805-818; DOI: https://doi.org/10.1105/tpc.106.046581
The hormone jasmonic acid (JA) functions in stress responses and plant development. This work identified the mitogen-activated protein kinase cascade, MKK3–MPK6, which is activated by JA, and indicates key roles for this kinase unit in the JA signaling pathway in processes such as root growth and negative regulation of the transcriptional activator ATMYC2.
Yoshioka, Hirofumi
- Open AccessCalcium-Dependent Protein Kinases Regulate the Production of Reactive Oxygen Species by Potato NADPH OxidaseMichie Kobayashi, Ikuko Ohura, Kazuhito Kawakita, Naohiko Yokota, Masayuki Fujiwara, Ko Shimamoto, Noriyuki Doke, Hirofumi YoshiokaPlant Cell Mar 2007, 19 (3) 1065-1080; DOI: https://doi.org/10.1105/tpc.106.048884
The NADPH oxidase RBOH and calcium-dependent protein kinases (CDPKs) are implicated in rapid production of reactive oxygen species, or oxidative burst, early in plant–pathogen interactions, but the regulatory mechanisms are unknown. The results of this work suggest that Ca2+-dependent phosphorylation of potato RBOHB by CDPK5 plays a central role in oxidative burst during pathogen infection.
Yu, Lin
- You have accessRestricted AccessThe N-Terminal Double-Stranded RNA Binding Domains of Arabidopsis HYPONASTIC LEAVES1 Are Sufficient for Pre-MicroRNA ProcessingFeijie Wu, Lin Yu, Wenguang Cao, Yanfei Mao, Zhongyuan Liu, Yuke HePlant Cell Mar 2007, 19 (3) 914-925; DOI: https://doi.org/10.1105/tpc.106.048637
Some double-stranded RNA binding proteins participate in miRNA biogenesis. A series of deletion mutants demonstrated that the N-terminal region of HYPONASTIC LEAVES1 with two double-stranded RNA binding domains is sufficient for miRNA biogenesis and capable of rescuing the hyl1 phenotype. This study also showed that the putative NLS is a true nuclear localization signal.
Yuan, Ming
- You have accessRestricted AccessArabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 Functions in Directional Cell Growth by Destabilizing Cortical MicrotubulesXia Wang, Lei Zhu, Baoquan Liu, Che Wang, Lifeng Jin, Qian Zhao, Ming YuanPlant Cell Mar 2007, 19 (3) 877-889; DOI: https://doi.org/10.1105/tpc.106.048579
Mutations in microtubule-associated proteins (MAPs) result in abnormal plant development and plant cell morphogenesis due to disruption of microtubule (MT) organization. This study identified Arabidopsis MAP18, which binds to microtubules and inhibits tubulin polymerization, destabilizes MTs, and plays an important role in the regulation of MT organization to determine directional plant cell growth.
Z
Zentgraf, Ulrike
- You have accessRestricted AccessThe Antagonist Function of Arabidopsis WRKY53 and ESR/ESP in Leaf Senescence Is Modulated by the Jasmonic and Salicylic Acid EquilibriumYing Miao, Ulrike ZentgrafPlant Cell Mar 2007, 19 (3) 819-830; DOI: https://doi.org/10.1105/tpc.106.042705
Crosstalk between salicylic acid (SA) and jasmonic acid (JA) in leaf senescence is not well-known. This work shows that the senescence-specific transcription factor WRKY53 is positively regulated by SA and negatively by JA signaling and that the antagonistically regulated EPITHIOSPECIFYING SENESCENCE REGULATOR functions in the cytoplasm in nitrile biosynthesis but in the nucleus as an interaction partner with WRKY53.
Zhao, Hongyu
- You have accessRestricted AccessAnalysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentJungeun Lee, Kun He, Viktor Stolc, Horim Lee, Pablo Figueroa, Ying Gao, Waraporn Tongprasit, Hongyu Zhao, Ilha Lee, Xing Wang DengPlant Cell Mar 2007, 19 (3) 731-749; DOI: https://doi.org/10.1105/tpc.106.047688
Light signals induce complex developmental programs in plants. The transcription factor HY5 acts downstream of multiple photoreceptors, but its target genes are not well known. Putative HY5 binding sites were mapped to 3894 genes with diverse developmental functions, including early light-responsive genes. HY5 appears to be an early regulator of the transcriptional cascades for photomorphogenesis.
Zhao, Jie
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
Zhao, Qian
- You have accessRestricted AccessArabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 Functions in Directional Cell Growth by Destabilizing Cortical MicrotubulesXia Wang, Lei Zhu, Baoquan Liu, Che Wang, Lifeng Jin, Qian Zhao, Ming YuanPlant Cell Mar 2007, 19 (3) 877-889; DOI: https://doi.org/10.1105/tpc.106.048579
Mutations in microtubule-associated proteins (MAPs) result in abnormal plant development and plant cell morphogenesis due to disruption of microtubule (MT) organization. This study identified Arabidopsis MAP18, which binds to microtubules and inhibits tubulin polymerization, destabilizes MTs, and plays an important role in the regulation of MT organization to determine directional plant cell growth.
Zhao, Xiao
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
Zheng, Huanquan
- You have accessRestricted AccessA Core Subunit of the RNA-Processing/Degrading Exosome Specifically Influences Cuticular Wax Biosynthesis in ArabidopsisTanya S. Hooker, Patricia Lam, Huanquan Zheng, Ljerka KunstPlant Cell Mar 2007, 19 (3) 904-913; DOI: https://doi.org/10.1105/tpc.106.049304
The cuticle, an extracellular matrix of cutin polyester and waxes, covers and protects plant aerial organs, but little is known about the regulation of cuticular wax deposition. Mutant analysis and gene isolation and characterization demonstrate that wax production is controlled, at least in part, by the mRNA stability of a repressor that is recognized and degraded by the CER7 ribonuclease.
Zhu, Lei
- You have accessRestricted AccessArabidopsis MICROTUBULE-ASSOCIATED PROTEIN18 Functions in Directional Cell Growth by Destabilizing Cortical MicrotubulesXia Wang, Lei Zhu, Baoquan Liu, Che Wang, Lifeng Jin, Qian Zhao, Ming YuanPlant Cell Mar 2007, 19 (3) 877-889; DOI: https://doi.org/10.1105/tpc.106.048579
Mutations in microtubule-associated proteins (MAPs) result in abnormal plant development and plant cell morphogenesis due to disruption of microtubule (MT) organization. This study identified Arabidopsis MAP18, which binds to microtubules and inhibits tubulin polymerization, destabilizes MTs, and plays an important role in the regulation of MT organization to determine directional plant cell growth.
Zhu, Lili
- Open AccessRice UDP-Glucose Pyrophosphorylase1 Is Essential for Pollen Callose Deposition and Its Cosuppression Results in a New Type of Thermosensitive Genic Male SterilityRongzhi Chen, Xiao Zhao, Zhe Shao, Zhe Wei, Yuanyuan Wang, Lili Zhu, Jie Zhao, Mengxiang Sun, Ruifeng He, Guangcun HePlant Cell Mar 2007, 19 (3) 847-861; DOI: https://doi.org/10.1105/tpc.106.044123
UDP-glucose pyrophosphorylase functions in carbohydrate metabolism and cell wall biosynthesis. Silencing of rice Ugp1 affects normal callose deposition during pollen mother cell (PMC) meiosis and leads to the degeneration of PMC at the early meiosis stage. This work demonstrates that rice Ugp1 is essential for PMC meiosis and microspore development.
Zimmer, Sara L.
- Open AccessIntegration of Chloroplast Nucleic Acid Metabolism into the Phosphate Deprivation Response in Chlamydomonas reinhardtiiShlomit Yehudai-Resheff, Sara L. Zimmer, Yutaka Komine, David B. SternPlant Cell Mar 2007, 19 (3) 1023-1038; DOI: https://doi.org/10.1105/tpc.106.045427
The role of the chloroplast as a phosphorus (P) reservoir in acclimation to P limitation is not well known. Polynucleotide phosphorylase consumes P through RNA decay, and while P deprivation usually stimulates RNA decay, in Chlamydomonas chloroplasts RNA levels rise but DNA levels fall during acclimation, suggesting that chloroplast polyploidy may be adaptive as a repository for P.