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Abstract
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Cooperative Action of Rhizobium meliloti Nodulation and Infection Mutants during the Process of Forming Mixed Infected Alfalfa Nodules.

D. Kapp, K. Niehaus, J. Quandt, P. Muller, A. Puhler
D. Kapp
University of Bielefeld, Faculty of Biology, Department of Genetics, Postbox 8640, D-4800 Bielefeld 1, Federal Republic of Germany.
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K. Niehaus
University of Bielefeld, Faculty of Biology, Department of Genetics, Postbox 8640, D-4800 Bielefeld 1, Federal Republic of Germany.
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J. Quandt
University of Bielefeld, Faculty of Biology, Department of Genetics, Postbox 8640, D-4800 Bielefeld 1, Federal Republic of Germany.
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P. Muller
University of Bielefeld, Faculty of Biology, Department of Genetics, Postbox 8640, D-4800 Bielefeld 1, Federal Republic of Germany.
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A. Puhler
University of Bielefeld, Faculty of Biology, Department of Genetics, Postbox 8640, D-4800 Bielefeld 1, Federal Republic of Germany.
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Published February 1990. DOI: https://doi.org/10.1105/tpc.2.2.139

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  • Copyright © 1996 by American Society of Plant Biologists

Abstract

Alfalfa plants co-inoculated with Rhizobium meliloti nodulation (Nod-) and infection mutants deficient in exopolysaccharide production (Inf-EPS-) formed mixed infected nodules that were capable of fixing atmospheric nitrogen. The formation of infected nodules was dependent on close contact between the inoculation partners. When the partners were separated by a filter, empty Fix- nodules were formed, suggesting that infection thread formation in alfalfa is dependent on signals from the nodulation and infection genes. In mixed infected nodules, both nodulation and infection mutants colonized the plant cells and differentiated into bacteroids. The formation of bacteroids was not dependent on cell-to-cell contact between the mutants. Immunogold/silver staining revealed that the ratio of the two mutants varied considerably in colonized plant cells following mixed inoculation. The introduction of an additional nif/fix mutation into one of the inoculation partners did not abolish nitrogen fixation in mixed infected nodules. The expression of nif D::lacZ fusions additionally demonstrated that mutations in the nodulation and infection genes did not prevent the nif genes from being expressed in the mutant bacteroids.

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Cooperative Action of Rhizobium meliloti Nodulation and Infection Mutants during the Process of Forming Mixed Infected Alfalfa Nodules.
D. Kapp, K. Niehaus, J. Quandt, P. Muller, A. Puhler
The Plant Cell Feb 1990, 2 (2) 139-151; DOI: 10.1105/tpc.2.2.139

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Cooperative Action of Rhizobium meliloti Nodulation and Infection Mutants during the Process of Forming Mixed Infected Alfalfa Nodules.
D. Kapp, K. Niehaus, J. Quandt, P. Muller, A. Puhler
The Plant Cell Feb 1990, 2 (2) 139-151; DOI: 10.1105/tpc.2.2.139
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The Plant Cell
Vol. 2, Issue 2
Feb 1990
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