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Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis

Jacob Krüger Jensen, Susanne Oxenbøll Sørensen, Jesper Harholt, Naomi Geshi, Yumiko Sakuragi, Isabel Møller, Joris Zandleven, Adriana J. Bernal, Niels Bjerg Jensen, Charlotte Sørensen, Markus Pauly, Gerrit Beldman, William G.T. Willats, Henrik Vibe Scheller
Jacob Krüger Jensen
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
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Susanne Oxenbøll Sørensen
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
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Jesper Harholt
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
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Naomi Geshi
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
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Yumiko Sakuragi
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
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Isabel Møller
bDepartment of Molecular Biology, Faculty of Science, University of Copenhagen, DK-1353 Copenhagen, Denmark
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Joris Zandleven
cLaboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, 6700 EV Wageningen, The Netherlands
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Adriana J. Bernal
bDepartment of Molecular Biology, Faculty of Science, University of Copenhagen, DK-1353 Copenhagen, Denmark
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Niels Bjerg Jensen
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
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Charlotte Sørensen
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
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Markus Pauly
dMax Planck Institute for Molecular Plant Physiology, D-14476 Golm, Germany
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Gerrit Beldman
cLaboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, 6700 EV Wageningen, The Netherlands
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William G.T. Willats
bDepartment of Molecular Biology, Faculty of Science, University of Copenhagen, DK-1353 Copenhagen, Denmark
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Henrik Vibe Scheller
aLaboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark
eFeedstocks Division, Joint Bioenergy Institute, Emeryville, California 94608
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Published May 2008. DOI: https://doi.org/10.1105/tpc.107.050906

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Abstract

Xylogalacturonan (XGA) is a class of pectic polysaccharide found in plant cell walls. The Arabidopsis thaliana locus At5g33290 encodes a predicted Type II membrane protein, and insertion mutants of the At5g33290 locus had decreased cell wall xylose. Immunological studies, enzymatic extraction of polysaccharides, monosaccharide linkage analysis, and oligosaccharide mass profiling were employed to identify the affected cell wall polymer. Pectic XGA was reduced to much lower levels in mutant than in wild-type leaves, indicating a role of At5g33290 in XGA biosynthesis. The mutated gene was designated xylogalacturonan deficient1 (xgd1). Transformation of the xgd1-1 mutant with the wild-type gene restored XGA to wild-type levels. XGD1 protein heterologously expressed in Nicotiana benthamiana catalyzed the transfer of xylose from UDP-xylose onto oligogalacturonides and endogenous acceptors. The products formed could be hydrolyzed with an XGA-specific hydrolase. These results confirm that the XGD1 protein is a XGA xylosyltransferase. The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis.

  • Received February 6, 2007.
  • Revised April 11, 2008.
  • Accepted April 21, 2008.
  • Published May 6, 2008.

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Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis
Jacob Krüger Jensen, Susanne Oxenbøll Sørensen, Jesper Harholt, Naomi Geshi, Yumiko Sakuragi, Isabel Møller, Joris Zandleven, Adriana J. Bernal, Niels Bjerg Jensen, Charlotte Sørensen, Markus Pauly, Gerrit Beldman, William G.T. Willats, Henrik Vibe Scheller
The Plant Cell May 2008, 20 (5) 1289-1302; DOI: 10.1105/tpc.107.050906

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Identification of a Xylogalacturonan Xylosyltransferase Involved in Pectin Biosynthesis in Arabidopsis
Jacob Krüger Jensen, Susanne Oxenbøll Sørensen, Jesper Harholt, Naomi Geshi, Yumiko Sakuragi, Isabel Møller, Joris Zandleven, Adriana J. Bernal, Niels Bjerg Jensen, Charlotte Sørensen, Markus Pauly, Gerrit Beldman, William G.T. Willats, Henrik Vibe Scheller
The Plant Cell May 2008, 20 (5) 1289-1302; DOI: 10.1105/tpc.107.050906
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The Plant Cell Online: 20 (5)
The Plant Cell
Vol. 20, Issue 5
May 2008
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