Table of Contents
A
Aki, Shiori
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Akman, Ozgur E.
- You have accessRestricted AccessPeeking into Pit Fields: A Multiple Twinning Model of Secondary Plasmodesmata Formation in TobaccoChristine Faulkner, Ozgur E. Akman, Karen Bell, Chris Jeffree, Karl OparkaPlant Cell Jun 2008, 20 (6) 1504-1518; DOI: https://doi.org/10.1105/tpc.107.056903
Pores called plasmodesmata that connect plant cells arise at cytokinesis and secondarily during wall extension. This work uses a range of imaging and computational approaches to show that secondary plasmodesmata arise in direct contact with existing plasmodesmata during radial wall extension.
Andriopoulou, Athina H.
- You have accessRestricted AccessSpermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in TobaccoPanagiotis N. Moschou, Konstantinos A. Paschalidis, Ioannis D. Delis, Athina H. Andriopoulou, George D. Lagiotis, Dimitrios I. Yakoumakis, Kalliopi A. Roubelakis-AngelakisPlant Cell Jun 2008, 20 (6) 1708-1724; DOI: https://doi.org/10.1105/tpc.108.059733
Polyamines play a protective role against stress challenges in plants. This work shows that abiotic stress induces spermidine secretion into the apoplast, where it is oxidized by polyamine oxidase. Depending on its levels and those of intracellular polyamines, the generated H2O2 induces either tolerance events or programmed cell death.
Aoyama, Takashi
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Arimura, Shin-ichi
- You have accessRestricted AccessArabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission SitesShin-ichi Arimura, Masaru Fujimoto, Yoko Doniwa, Naoki Kadoya, Mikio Nakazono, Wataru Sakamoto, Nobuhiro TsutsumiPlant Cell Jun 2008, 20 (6) 1555-1566; DOI: https://doi.org/10.1105/tpc.108.058578
Mitochondrial fission is a fundamental but poorly understood process in plant cells. DYNAMIN-RELATED PROTEIN3A is a conserved eukaryotic mitochondrial fission factor found in Arabidopsis that forms a ring around and severs mitochondria. Here, we identify a novel plant-specific factor, ELONGATED MITOCHONDRIA1, that is required for the correct localization of DRP3A.
Asami, Tadao
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
B
Barthélémy, Julien
- You have accessRestricted AccessHigh-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in ArabidopsisElisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, Julien Barthélémy, Jean-Christophe PalauquiPlant Cell Jun 2008, 20 (6) 1494-1503; DOI: https://doi.org/10.1105/tpc.107.056069
This work images phloem development using a remarkable improvement in plant tissue staining that, in combination with confocal microscopy, reveals three-dimensional cellular organization of plant tissue with a precision that formerly required tissue sectioning. Furthermore, coupled with staining for β-glucuronidase activity, this method shows gene expression with single-cell resolution.
Bauby, Hélène
- You have accessRestricted AccessHigh-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in ArabidopsisElisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, Julien Barthélémy, Jean-Christophe PalauquiPlant Cell Jun 2008, 20 (6) 1494-1503; DOI: https://doi.org/10.1105/tpc.107.056069
This work images phloem development using a remarkable improvement in plant tissue staining that, in combination with confocal microscopy, reveals three-dimensional cellular organization of plant tissue with a precision that formerly required tissue sectioning. Furthermore, coupled with staining for β-glucuronidase activity, this method shows gene expression with single-cell resolution.
Bell, Karen
- You have accessRestricted AccessPeeking into Pit Fields: A Multiple Twinning Model of Secondary Plasmodesmata Formation in TobaccoChristine Faulkner, Ozgur E. Akman, Karen Bell, Chris Jeffree, Karl OparkaPlant Cell Jun 2008, 20 (6) 1504-1518; DOI: https://doi.org/10.1105/tpc.107.056903
Pores called plasmodesmata that connect plant cells arise at cytokinesis and secondarily during wall extension. This work uses a range of imaging and computational approaches to show that secondary plasmodesmata arise in direct contact with existing plasmodesmata during radial wall extension.
Berthold, Peter
- You have accessRestricted AccessChannelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-Induced DepolarizationPeter Berthold, Satoshi P. Tsunoda, Oliver P. Ernst, Wolfgang Mages, Dietrich Gradmann, Peter HegemannPlant Cell Jun 2008, 20 (6) 1665-1677; DOI: https://doi.org/10.1105/tpc.108.057919
This study reanalyzed channelrhodopsin-1 (CHR1)–based photocurrents in Chlamydomonas and demonstrated that, in addition to H+, ChR1 also conducts other cations. This conductance enables the alga to orient in the light even at alkaline pH.
Bevan, Michael W.
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Bigler, Laurent
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Bingham, Scott
- You have accessRestricted AccessArabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of PeroxisomesMatthew J. Lingard, Satinder K. Gidda, Scott Bingham, Steven J. Rothstein, Robert T. Mullen, Richard N. TreleasePlant Cell Jun 2008, 20 (6) 1567-1585; DOI: https://doi.org/10.1105/tpc.107.057679
This study examines the molecular interactions of PEROXIN11 isoforms with each other, with FISSION1 isoforms, and with DYNAMIN-RELATED PROTEIN3A (DRP3A) in peroxisome replication during cell division. Gene silencing and protein interaction experiments show that these proteins play distinct but overlapping roles in the elongation and fission of replicating peroxisomes.
Birney, Ewan
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Bosch, Dirk
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Brkljacic, Jelena
- You have accessRestricted AccessTwo Distinct Interacting Classes of Nuclear Envelope–Associated Coiled-Coil Proteins Are Required for the Tissue-Specific Nuclear Envelope Targeting of Arabidopsis RanGAPQiao Zhao, Jelena Brkljacic, Iris MeierPlant Cell Jun 2008, 20 (6) 1639-1651; DOI: https://doi.org/10.1105/tpc.108.059220
Tandem affinity purification and mass spectrometry were used to identify a family of novel coiled-coil proteins required for nuclear envelope association of RanGAP1 in Arabidopsis root tip cells. The findings suggest an unanticipated complexity of RanGAP nuclear envelope targeting in higher plant cells, involving at least two families of likely plant-specific nuclear pore–associated proteins.
Burgert, Ingo
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
C
Castillon, Alicia
- You have accessRestricted AccessLight-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 from Arabidopsis Depend upon Its Direct Physical Interactions with Photoactivated PhytochromesHui Shen, Ling Zhu, Alicia Castillon, Manoj Majee, Bruce Downie, Enamul HuqPlant Cell Jun 2008, 20 (6) 1586-1602; DOI: https://doi.org/10.1105/tpc.108.060020
PHYTOCHROME INTERACTING FACTOR1 is rapidly phosphorylated, ubiquitinated, and degraded under red and far-red light. Overexpression of a light-stable truncated PIF1 showed a constitutive photomorphogenic phenotype in the dark, suggesting that light-induced proteolytic degradation of negative regulators (e.g. PIFs) might be sufficient to promote photomorphogenesis.
Cavalier, David M.
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Chen, Fangfang
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
Chen, Haodong
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
Chen, Songbiao
- You have accessRestricted AccessSPIN1, a K Homology Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11, Is Involved in Flowering Time Control in RiceMiguel E. Vega-Sánchez, Lirong Zeng, Songbiao Chen, Hei Leung, Guo-Liang WangPlant Cell Jun 2008, 20 (6) 1456-1469; DOI: https://doi.org/10.1105/tpc.108.058610
Both RNA processing and ubiquitination play important roles in the regulation of flowering. This work implicates the E3 ligase SPOTTED LEAF11 and its substrate SPIN1, a nuclear RNA/DNA binding protein of the STAR family, in rice flowering time control. This study links ubiquitination and RNA/DNA metabolism in flowering time regulation in plants.
Choi, Seungjin
- You have accessRestricted AccessArabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence MotifsDong Wook Lee, Jong Kyoung Kim, Sumin Lee, Seungjin Choi, Sanguk Kim, Inhwan HwangPlant Cell Jun 2008, 20 (6) 1603-1622; DOI: https://doi.org/10.1105/tpc.108.060541
This work examines sequences critical for chloroplast protein import by alanine substitution mutagenesis of proteins with very different transit peptide sequences. This approach is complemented by bioinformatic grouping of peptides and motif identification within subgroups, producing a highly accurate algorithm to predict chloroplast localization.
Couchman, Matthew
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
D
D'Eustachio, Peter
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Delis, Ioannis D.
- You have accessRestricted AccessSpermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in TobaccoPanagiotis N. Moschou, Konstantinos A. Paschalidis, Ioannis D. Delis, Athina H. Andriopoulou, George D. Lagiotis, Dimitrios I. Yakoumakis, Kalliopi A. Roubelakis-AngelakisPlant Cell Jun 2008, 20 (6) 1708-1724; DOI: https://doi.org/10.1105/tpc.108.059733
Polyamines play a protective role against stress challenges in plants. This work shows that abiotic stress induces spermidine secretion into the apoplast, where it is oxidized by polyamine oxidase. Depending on its levels and those of intracellular polyamines, the generated H2O2 induces either tolerance events or programmed cell death.
Deng, Xing Wang
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
Diet, Anouck
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Doniwa, Yoko
- You have accessRestricted AccessArabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission SitesShin-ichi Arimura, Masaru Fujimoto, Yoko Doniwa, Naoki Kadoya, Mikio Nakazono, Wataru Sakamoto, Nobuhiro TsutsumiPlant Cell Jun 2008, 20 (6) 1555-1566; DOI: https://doi.org/10.1105/tpc.108.058578
Mitochondrial fission is a fundamental but poorly understood process in plant cells. DYNAMIN-RELATED PROTEIN3A is a conserved eukaryotic mitochondrial fission factor found in Arabidopsis that forms a ring around and severs mitochondria. Here, we identify a novel plant-specific factor, ELONGATED MITOCHONDRIA1, that is required for the correct localization of DRP3A.
Doonan, John H.
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Downie, Bruce
- You have accessRestricted AccessLight-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 from Arabidopsis Depend upon Its Direct Physical Interactions with Photoactivated PhytochromesHui Shen, Ling Zhu, Alicia Castillon, Manoj Majee, Bruce Downie, Enamul HuqPlant Cell Jun 2008, 20 (6) 1586-1602; DOI: https://doi.org/10.1105/tpc.108.060020
PHYTOCHROME INTERACTING FACTOR1 is rapidly phosphorylated, ubiquitinated, and degraded under red and far-red light. Overexpression of a light-stable truncated PIF1 showed a constitutive photomorphogenic phenotype in the dark, suggesting that light-induced proteolytic degradation of negative regulators (e.g. PIFs) might be sufficient to promote photomorphogenesis.
Dubreucq, Bertrand
- You have accessRestricted AccessHigh-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in ArabidopsisElisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, Julien Barthélémy, Jean-Christophe PalauquiPlant Cell Jun 2008, 20 (6) 1494-1503; DOI: https://doi.org/10.1105/tpc.107.056069
This work images phloem development using a remarkable improvement in plant tissue staining that, in combination with confocal microscopy, reveals three-dimensional cellular organization of plant tissue with a precision that formerly required tissue sectioning. Furthermore, coupled with staining for β-glucuronidase activity, this method shows gene expression with single-cell resolution.
E
Eckardt, Nancy A.
- You have accessRestricted AccessRole of Xyloglucan in Primary Cell WallsNancy A. EckardtPlant Cell Jun 2008, 20 (6) 1421-1422; DOI: https://doi.org/10.1105/tpc.108.061382
- You have accessRestricted AccessHigh-Resolution Three-Dimensional Imaging of Plant TissuesNancy A. EckardtPlant Cell Jun 2008, 20 (6) 1423; DOI: https://doi.org/10.1105/tpc.108.200610
Ernst, Oliver P.
- You have accessRestricted AccessChannelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-Induced DepolarizationPeter Berthold, Satoshi P. Tsunoda, Oliver P. Ernst, Wolfgang Mages, Dietrich Gradmann, Peter HegemannPlant Cell Jun 2008, 20 (6) 1665-1677; DOI: https://doi.org/10.1105/tpc.108.057919
This study reanalyzed channelrhodopsin-1 (CHR1)–based photocurrents in Chlamydomonas and demonstrated that, in addition to H+, ChR1 also conducts other cations. This conductance enables the alga to orient in the light even at alkaline pH.
F
Faulkner, Christine
- You have accessRestricted AccessPeeking into Pit Fields: A Multiple Twinning Model of Secondary Plasmodesmata Formation in TobaccoChristine Faulkner, Ozgur E. Akman, Karen Bell, Chris Jeffree, Karl OparkaPlant Cell Jun 2008, 20 (6) 1504-1518; DOI: https://doi.org/10.1105/tpc.107.056903
Pores called plasmodesmata that connect plant cells arise at cytokinesis and secondarily during wall extension. This work uses a range of imaging and computational approaches to show that secondary plasmodesmata arise in direct contact with existing plasmodesmata during radial wall extension.
Feng, Suhua
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
Freshour, Glenn
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Frey, Beat
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Fujimoto, Masaru
- You have accessRestricted AccessArabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission SitesShin-ichi Arimura, Masaru Fujimoto, Yoko Doniwa, Naoki Kadoya, Mikio Nakazono, Wataru Sakamoto, Nobuhiro TsutsumiPlant Cell Jun 2008, 20 (6) 1555-1566; DOI: https://doi.org/10.1105/tpc.108.058578
Mitochondrial fission is a fundamental but poorly understood process in plant cells. DYNAMIN-RELATED PROTEIN3A is a conserved eukaryotic mitochondrial fission factor found in Arabidopsis that forms a ring around and severs mitochondria. Here, we identify a novel plant-specific factor, ELONGATED MITOCHONDRIA1, that is required for the correct localization of DRP3A.
Fujita, Miki
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Fujita, Yasunari
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
G
Gidda, Satinder K.
- You have accessRestricted AccessArabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of PeroxisomesMatthew J. Lingard, Satinder K. Gidda, Scott Bingham, Steven J. Rothstein, Robert T. Mullen, Richard N. TreleasePlant Cell Jun 2008, 20 (6) 1567-1585; DOI: https://doi.org/10.1105/tpc.107.057679
This study examines the molecular interactions of PEROXIN11 isoforms with each other, with FISSION1 isoforms, and with DYNAMIN-RELATED PROTEIN3A (DRP3A) in peroxisome replication during cell division. Gene silencing and protein interaction experiments show that these proteins play distinct but overlapping roles in the elongation and fission of replicating peroxisomes.
Gradmann, Dietrich
- You have accessRestricted AccessChannelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-Induced DepolarizationPeter Berthold, Satoshi P. Tsunoda, Oliver P. Ernst, Wolfgang Mages, Dietrich Gradmann, Peter HegemannPlant Cell Jun 2008, 20 (6) 1665-1677; DOI: https://doi.org/10.1105/tpc.108.057919
This study reanalyzed channelrhodopsin-1 (CHR1)–based photocurrents in Chlamydomonas and demonstrated that, in addition to H+, ChR1 also conducts other cations. This conductance enables the alga to orient in the light even at alkaline pH.
Grandjean, Olivier
- You have accessRestricted AccessHigh-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in ArabidopsisElisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, Julien Barthélémy, Jean-Christophe PalauquiPlant Cell Jun 2008, 20 (6) 1494-1503; DOI: https://doi.org/10.1105/tpc.107.056069
This work images phloem development using a remarkable improvement in plant tissue staining that, in combination with confocal microscopy, reveals three-dimensional cellular organization of plant tissue with a precision that formerly required tissue sectioning. Furthermore, coupled with staining for β-glucuronidase activity, this method shows gene expression with single-cell resolution.
Gu, Hongya
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
H
Hahn, Michael G.
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
- Open AccessAnalysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich MucilageRobin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn, A. Lacey SamuelsPlant Cell Jun 2008, 20 (6) 1623-1638; DOI: https://doi.org/10.1105/tpc.108.058842
Golgi stacks in Arabidopsis seed coat cells produce mucilage synchronously at a specific stage of differentiation. The morphology of stacks was dependent on the volume of mucilage produced, but the number was not, suggesting that proliferation of stacks is developmentally programmed. Mucilage-producing Golgi stacks were randomly distributed in the cell and not clustered at the site of secretion.
Han, Pei
- Open AccessMutation of Arabidopsis BARD1 Causes Meristem Defects by Failing to Confine WUSCHEL Expression to the Organizing CenterPei Han, Qing Li, Yu-Xian ZhuPlant Cell Jun 2008, 20 (6) 1482-1493; DOI: https://doi.org/10.1105/tpc.108.058867
BARD1 represses the transcriptional activity of WUSCHEL, a gene essential for maintenance of stem cell fate in Arabidopsis. In a bard1-null mutant, severe shoot apical meristem defects are observed as a result of WUSCHEL expression outside of the meristem organizing center. BARD1 may inhibit a chromatin-remodeling process vital for WUS expression.
Haughn, George W.
- Open AccessAnalysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich MucilageRobin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn, A. Lacey SamuelsPlant Cell Jun 2008, 20 (6) 1623-1638; DOI: https://doi.org/10.1105/tpc.108.058842
Golgi stacks in Arabidopsis seed coat cells produce mucilage synchronously at a specific stage of differentiation. The morphology of stacks was dependent on the volume of mucilage produced, but the number was not, suggesting that proliferation of stacks is developmentally programmed. Mucilage-producing Golgi stacks were randomly distributed in the cell and not clustered at the site of secretion.
Hegemann, Peter
- You have accessRestricted AccessChannelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-Induced DepolarizationPeter Berthold, Satoshi P. Tsunoda, Oliver P. Ernst, Wolfgang Mages, Dietrich Gradmann, Peter HegemannPlant Cell Jun 2008, 20 (6) 1665-1677; DOI: https://doi.org/10.1105/tpc.108.057919
This study reanalyzed channelrhodopsin-1 (CHR1)–based photocurrents in Chlamydomonas and demonstrated that, in addition to H+, ChR1 also conducts other cations. This conductance enables the alga to orient in the light even at alkaline pH.
Helsper, Johannes
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Henquet, Maurice
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Higgins, Janet
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Hofmann, Nancy
- You have accessRestricted AccessAbscisic Acid–Mediated Suppression of Systemic Acquired Resistance SignalingNancy HofmannPlant Cell Jun 2008, 20 (6) 1425; DOI: https://doi.org/10.1105/tpc.108.200612
Huq, Enamul
- You have accessRestricted AccessLight-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 from Arabidopsis Depend upon Its Direct Physical Interactions with Photoactivated PhytochromesHui Shen, Ling Zhu, Alicia Castillon, Manoj Majee, Bruce Downie, Enamul HuqPlant Cell Jun 2008, 20 (6) 1586-1602; DOI: https://doi.org/10.1105/tpc.108.060020
PHYTOCHROME INTERACTING FACTOR1 is rapidly phosphorylated, ubiquitinated, and degraded under red and far-red light. Overexpression of a light-stable truncated PIF1 showed a constitutive photomorphogenic phenotype in the dark, suggesting that light-induced proteolytic degradation of negative regulators (e.g. PIFs) might be sufficient to promote photomorphogenesis.
Hwang, Inhwan
- You have accessRestricted AccessArabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence MotifsDong Wook Lee, Jong Kyoung Kim, Sumin Lee, Seungjin Choi, Sanguk Kim, Inhwan HwangPlant Cell Jun 2008, 20 (6) 1603-1622; DOI: https://doi.org/10.1105/tpc.108.060541
This work examines sequences critical for chloroplast protein import by alanine substitution mutagenesis of proteins with very different transit peptide sequences. This approach is complemented by bioinformatic grouping of peptides and motif identification within subgroups, producing a highly accurate algorithm to predict chloroplast localization.
I
Ishikawa, Atsushi
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
J
Jeffree, Chris
- You have accessRestricted AccessPeeking into Pit Fields: A Multiple Twinning Model of Secondary Plasmodesmata Formation in TobaccoChristine Faulkner, Ozgur E. Akman, Karen Bell, Chris Jeffree, Karl OparkaPlant Cell Jun 2008, 20 (6) 1504-1518; DOI: https://doi.org/10.1105/tpc.107.056903
Pores called plasmodesmata that connect plant cells arise at cytokinesis and secondarily during wall extension. This work uses a range of imaging and computational approaches to show that secondary plasmodesmata arise in direct contact with existing plasmodesmata during radial wall extension.
Jikumaru, Yusuke
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
K
Kadoya, Naoki
- You have accessRestricted AccessArabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission SitesShin-ichi Arimura, Masaru Fujimoto, Yoko Doniwa, Naoki Kadoya, Mikio Nakazono, Wataru Sakamoto, Nobuhiro TsutsumiPlant Cell Jun 2008, 20 (6) 1555-1566; DOI: https://doi.org/10.1105/tpc.108.058578
Mitochondrial fission is a fundamental but poorly understood process in plant cells. DYNAMIN-RELATED PROTEIN3A is a conserved eukaryotic mitochondrial fission factor found in Arabidopsis that forms a ring around and severs mitochondria. Here, we identify a novel plant-specific factor, ELONGATED MITOCHONDRIA1, that is required for the correct localization of DRP3A.
Keegstra, Kenneth
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Kidokoro, Satoshi
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Kim, Jong Kyoung
- You have accessRestricted AccessArabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence MotifsDong Wook Lee, Jong Kyoung Kim, Sumin Lee, Seungjin Choi, Sanguk Kim, Inhwan HwangPlant Cell Jun 2008, 20 (6) 1603-1622; DOI: https://doi.org/10.1105/tpc.108.060541
This work examines sequences critical for chloroplast protein import by alanine substitution mutagenesis of proteins with very different transit peptide sequences. This approach is complemented by bioinformatic grouping of peptides and motif identification within subgroups, producing a highly accurate algorithm to predict chloroplast localization.
Kim, Sanguk
- You have accessRestricted AccessArabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence MotifsDong Wook Lee, Jong Kyoung Kim, Sumin Lee, Seungjin Choi, Sanguk Kim, Inhwan HwangPlant Cell Jun 2008, 20 (6) 1603-1622; DOI: https://doi.org/10.1105/tpc.108.060541
This work examines sequences critical for chloroplast protein import by alanine substitution mutagenesis of proteins with very different transit peptide sequences. This approach is complemented by bioinformatic grouping of peptides and motif identification within subgroups, producing a highly accurate algorithm to predict chloroplast localization.
Klein, Markus
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Kudo, Toshiaki
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
Kuhn, Benjamin M.
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
L
Lagiotis, George D.
- You have accessRestricted AccessSpermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in TobaccoPanagiotis N. Moschou, Konstantinos A. Paschalidis, Ioannis D. Delis, Athina H. Andriopoulou, George D. Lagiotis, Dimitrios I. Yakoumakis, Kalliopi A. Roubelakis-AngelakisPlant Cell Jun 2008, 20 (6) 1708-1724; DOI: https://doi.org/10.1105/tpc.108.059733
Polyamines play a protective role against stress challenges in plants. This work shows that abiotic stress induces spermidine secretion into the apoplast, where it is oxidized by polyamine oxidase. Depending on its levels and those of intracellular polyamines, the generated H2O2 induces either tolerance events or programmed cell death.
Lee, Dong Wook
- You have accessRestricted AccessArabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence MotifsDong Wook Lee, Jong Kyoung Kim, Sumin Lee, Seungjin Choi, Sanguk Kim, Inhwan HwangPlant Cell Jun 2008, 20 (6) 1603-1622; DOI: https://doi.org/10.1105/tpc.108.060541
This work examines sequences critical for chloroplast protein import by alanine substitution mutagenesis of proteins with very different transit peptide sequences. This approach is complemented by bioinformatic grouping of peptides and motif identification within subgroups, producing a highly accurate algorithm to predict chloroplast localization.
Lee, Sumin
- You have accessRestricted AccessArabidopsis Nuclear-Encoded Plastid Transit Peptides Contain Multiple Sequence Subgroups with Distinctive Chloroplast-Targeting Sequence MotifsDong Wook Lee, Jong Kyoung Kim, Sumin Lee, Seungjin Choi, Sanguk Kim, Inhwan HwangPlant Cell Jun 2008, 20 (6) 1603-1622; DOI: https://doi.org/10.1105/tpc.108.060541
This work examines sequences critical for chloroplast protein import by alanine substitution mutagenesis of proteins with very different transit peptide sequences. This approach is complemented by bioinformatic grouping of peptides and motif identification within subgroups, producing a highly accurate algorithm to predict chloroplast localization.
Lehle, Ludwig
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Leiber, Ruth-Maria
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Lerouxel, Olivier
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Leung, Hei
- You have accessRestricted AccessSPIN1, a K Homology Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11, Is Involved in Flowering Time Control in RiceMiguel E. Vega-Sánchez, Lirong Zeng, Songbiao Chen, Hei Leung, Guo-Liang WangPlant Cell Jun 2008, 20 (6) 1456-1469; DOI: https://doi.org/10.1105/tpc.108.058610
Both RNA processing and ubiquitination play important roles in the regulation of flowering. This work implicates the E3 ligase SPOTTED LEAF11 and its substrate SPIN1, a nuclear RNA/DNA binding protein of the STAR family, in rice flowering time control. This study links ubiquitination and RNA/DNA metabolism in flowering time regulation in plants.
Li, Qing
- Open AccessMutation of Arabidopsis BARD1 Causes Meristem Defects by Failing to Confine WUSCHEL Expression to the Organizing CenterPei Han, Qing Li, Yu-Xian ZhuPlant Cell Jun 2008, 20 (6) 1482-1493; DOI: https://doi.org/10.1105/tpc.108.058867
BARD1 represses the transcriptional activity of WUSCHEL, a gene essential for maintenance of stem cell fate in Arabidopsis. In a bard1-null mutant, severe shoot apical meristem defects are observed as a result of WUSCHEL expression outside of the meristem organizing center. BARD1 may inhibit a chromatin-remodeling process vital for WUS expression.
Lingard, Matthew J.
- You have accessRestricted AccessArabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of PeroxisomesMatthew J. Lingard, Satinder K. Gidda, Scott Bingham, Steven J. Rothstein, Robert T. Mullen, Richard N. TreleasePlant Cell Jun 2008, 20 (6) 1567-1585; DOI: https://doi.org/10.1105/tpc.107.057679
This study examines the molecular interactions of PEROXIN11 isoforms with each other, with FISSION1 isoforms, and with DYNAMIN-RELATED PROTEIN3A (DRP3A) in peroxisome replication during cell division. Gene silencing and protein interaction experiments show that these proteins play distinct but overlapping roles in the elongation and fission of replicating peroxisomes.
Liu, Jingjing
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Luo, Guo
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
M
Mach, Jennifer
- You have accessRestricted AccessUbiquitin Ligation RINGs Twice: Redundant Control of Plant Processes by E3 Ubiquitin LigasesJennifer MachPlant Cell Jun 2008, 20 (6) 1424; DOI: https://doi.org/10.1105/tpc.108.200611
Mages, Wolfgang
- You have accessRestricted AccessChannelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-Induced DepolarizationPeter Berthold, Satoshi P. Tsunoda, Oliver P. Ernst, Wolfgang Mages, Dietrich Gradmann, Peter HegemannPlant Cell Jun 2008, 20 (6) 1665-1677; DOI: https://doi.org/10.1105/tpc.108.057919
This study reanalyzed channelrhodopsin-1 (CHR1)–based photocurrents in Chlamydomonas and demonstrated that, in addition to H+, ChR1 also conducts other cations. This conductance enables the alga to orient in the light even at alkaline pH.
Majee, Manoj
- You have accessRestricted AccessLight-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 from Arabidopsis Depend upon Its Direct Physical Interactions with Photoactivated PhytochromesHui Shen, Ling Zhu, Alicia Castillon, Manoj Majee, Bruce Downie, Enamul HuqPlant Cell Jun 2008, 20 (6) 1586-1602; DOI: https://doi.org/10.1105/tpc.108.060020
PHYTOCHROME INTERACTING FACTOR1 is rapidly phosphorylated, ubiquitinated, and degraded under red and far-red light. Overexpression of a light-stable truncated PIF1 showed a constitutive photomorphogenic phenotype in the dark, suggesting that light-induced proteolytic degradation of negative regulators (e.g. PIFs) might be sufficient to promote photomorphogenesis.
Maruyama, Kyonoshin
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Maruyama-Nakashita, Akiko
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
McCall, Chad
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
McFarlane, Heather E.
- Open AccessAnalysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich MucilageRobin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn, A. Lacey SamuelsPlant Cell Jun 2008, 20 (6) 1623-1638; DOI: https://doi.org/10.1105/tpc.108.058842
Golgi stacks in Arabidopsis seed coat cells produce mucilage synchronously at a specific stage of differentiation. The morphology of stacks was dependent on the volume of mucilage produced, but the number was not, suggesting that proliferation of stacks is developmentally programmed. Mucilage-producing Golgi stacks were randomly distributed in the cell and not clustered at the site of secretion.
Meier, Iris
- You have accessRestricted AccessTwo Distinct Interacting Classes of Nuclear Envelope–Associated Coiled-Coil Proteins Are Required for the Tissue-Specific Nuclear Envelope Targeting of Arabidopsis RanGAPQiao Zhao, Jelena Brkljacic, Iris MeierPlant Cell Jun 2008, 20 (6) 1639-1651; DOI: https://doi.org/10.1105/tpc.108.059220
Tandem affinity purification and mass spectrometry were used to identify a family of novel coiled-coil proteins required for nuclear envelope association of RanGAP1 in Arabidopsis root tip cells. The findings suggest an unanticipated complexity of RanGAP nuclear envelope targeting in higher plant cells, involving at least two families of likely plant-specific nuclear pore–associated proteins.
Miyazono, Ken-ichi
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Molthoff, Jos
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Morris, Richard J.
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Moschou, Panagiotis N.
- You have accessRestricted AccessSpermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in TobaccoPanagiotis N. Moschou, Konstantinos A. Paschalidis, Ioannis D. Delis, Athina H. Andriopoulou, George D. Lagiotis, Dimitrios I. Yakoumakis, Kalliopi A. Roubelakis-AngelakisPlant Cell Jun 2008, 20 (6) 1708-1724; DOI: https://doi.org/10.1105/tpc.108.059733
Polyamines play a protective role against stress challenges in plants. This work shows that abiotic stress induces spermidine secretion into the apoplast, where it is oxidized by polyamine oxidase. Depending on its levels and those of intracellular polyamines, the generated H2O2 induces either tolerance events or programmed cell death.
Mullen, Robert T.
- You have accessRestricted AccessArabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of PeroxisomesMatthew J. Lingard, Satinder K. Gidda, Scott Bingham, Steven J. Rothstein, Robert T. Mullen, Richard N. TreleasePlant Cell Jun 2008, 20 (6) 1567-1585; DOI: https://doi.org/10.1105/tpc.107.057679
This study examines the molecular interactions of PEROXIN11 isoforms with each other, with FISSION1 isoforms, and with DYNAMIN-RELATED PROTEIN3A (DRP3A) in peroxisome replication during cell division. Gene silencing and protein interaction experiments show that these proteins play distinct but overlapping roles in the elongation and fission of replicating peroxisomes.
N
Nakashita, Hideo
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
Nakazono, Mikio
- You have accessRestricted AccessArabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission SitesShin-ichi Arimura, Masaru Fujimoto, Yoko Doniwa, Naoki Kadoya, Mikio Nakazono, Wataru Sakamoto, Nobuhiro TsutsumiPlant Cell Jun 2008, 20 (6) 1555-1566; DOI: https://doi.org/10.1105/tpc.108.058578
Mitochondrial fission is a fundamental but poorly understood process in plant cells. DYNAMIN-RELATED PROTEIN3A is a conserved eukaryotic mitochondrial fission factor found in Arabidopsis that forms a ring around and severs mitochondria. Here, we identify a novel plant-specific factor, ELONGATED MITOCHONDRIA1, that is required for the correct localization of DRP3A.
Neumetzler, Lutz
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
O
Oka, Atsuhiro
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Oparka, Karl
- You have accessRestricted AccessPeeking into Pit Fields: A Multiple Twinning Model of Secondary Plasmodesmata Formation in TobaccoChristine Faulkner, Ozgur E. Akman, Karen Bell, Chris Jeffree, Karl OparkaPlant Cell Jun 2008, 20 (6) 1504-1518; DOI: https://doi.org/10.1105/tpc.107.056903
Pores called plasmodesmata that connect plant cells arise at cytokinesis and secondarily during wall extension. This work uses a range of imaging and computational approaches to show that secondary plasmodesmata arise in direct contact with existing plasmodesmata during radial wall extension.
P
Palauqui, Jean-Christophe
- You have accessRestricted AccessHigh-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in ArabidopsisElisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, Julien Barthélémy, Jean-Christophe PalauquiPlant Cell Jun 2008, 20 (6) 1494-1503; DOI: https://doi.org/10.1105/tpc.107.056069
This work images phloem development using a remarkable improvement in plant tissue staining that, in combination with confocal microscopy, reveals three-dimensional cellular organization of plant tissue with a precision that formerly required tissue sectioning. Furthermore, coupled with staining for β-glucuronidase activity, this method shows gene expression with single-cell resolution.
Paschalidis, Konstantinos A.
- You have accessRestricted AccessSpermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in TobaccoPanagiotis N. Moschou, Konstantinos A. Paschalidis, Ioannis D. Delis, Athina H. Andriopoulou, George D. Lagiotis, Dimitrios I. Yakoumakis, Kalliopi A. Roubelakis-AngelakisPlant Cell Jun 2008, 20 (6) 1708-1724; DOI: https://doi.org/10.1105/tpc.108.059733
Polyamines play a protective role against stress challenges in plants. This work shows that abiotic stress induces spermidine secretion into the apoplast, where it is oxidized by polyamine oxidase. Depending on its levels and those of intracellular polyamines, the generated H2O2 induces either tolerance events or programmed cell death.
Pauly, Markus
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Pollmann, Stephan
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Q
Qin, Feng
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Qin, Genji
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Qu, Li-Jia
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
R
Raikhel, Natasha V.
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Reinecke, Antje
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Ringli, Christoph
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Rothstein, Steven J.
- You have accessRestricted AccessArabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of PeroxisomesMatthew J. Lingard, Satinder K. Gidda, Scott Bingham, Steven J. Rothstein, Robert T. Mullen, Richard N. TreleasePlant Cell Jun 2008, 20 (6) 1567-1585; DOI: https://doi.org/10.1105/tpc.107.057679
This study examines the molecular interactions of PEROXIN11 isoforms with each other, with FISSION1 isoforms, and with DYNAMIN-RELATED PROTEIN3A (DRP3A) in peroxisome replication during cell division. Gene silencing and protein interaction experiments show that these proteins play distinct but overlapping roles in the elongation and fission of replicating peroxisomes.
Roubelakis-Angelakis, Kalliopi A.
- You have accessRestricted AccessSpermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in TobaccoPanagiotis N. Moschou, Konstantinos A. Paschalidis, Ioannis D. Delis, Athina H. Andriopoulou, George D. Lagiotis, Dimitrios I. Yakoumakis, Kalliopi A. Roubelakis-AngelakisPlant Cell Jun 2008, 20 (6) 1708-1724; DOI: https://doi.org/10.1105/tpc.108.059733
Polyamines play a protective role against stress challenges in plants. This work shows that abiotic stress induces spermidine secretion into the apoplast, where it is oxidized by polyamine oxidase. Depending on its levels and those of intracellular polyamines, the generated H2O2 induces either tolerance events or programmed cell death.
Rouwendal, Gerard
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Runions, John
- You have accessRestricted AccessHigh-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in ArabidopsisElisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, Julien Barthélémy, Jean-Christophe PalauquiPlant Cell Jun 2008, 20 (6) 1494-1503; DOI: https://doi.org/10.1105/tpc.107.056069
This work images phloem development using a remarkable improvement in plant tissue staining that, in combination with confocal microscopy, reveals three-dimensional cellular organization of plant tissue with a precision that formerly required tissue sectioning. Furthermore, coupled with staining for β-glucuronidase activity, this method shows gene expression with single-cell resolution.
S
Sakaguchi, Norihiro
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Sakamoto, Wataru
- You have accessRestricted AccessArabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission SitesShin-ichi Arimura, Masaru Fujimoto, Yoko Doniwa, Naoki Kadoya, Mikio Nakazono, Wataru Sakamoto, Nobuhiro TsutsumiPlant Cell Jun 2008, 20 (6) 1555-1566; DOI: https://doi.org/10.1105/tpc.108.058578
Mitochondrial fission is a fundamental but poorly understood process in plant cells. DYNAMIN-RELATED PROTEIN3A is a conserved eukaryotic mitochondrial fission factor found in Arabidopsis that forms a ring around and severs mitochondria. Here, we identify a novel plant-specific factor, ELONGATED MITOCHONDRIA1, that is required for the correct localization of DRP3A.
Sakuma, Yoh
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Samuels, A. Lacey
- Open AccessAnalysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich MucilageRobin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn, A. Lacey SamuelsPlant Cell Jun 2008, 20 (6) 1623-1638; DOI: https://doi.org/10.1105/tpc.108.058842
Golgi stacks in Arabidopsis seed coat cells produce mucilage synchronously at a specific stage of differentiation. The morphology of stacks was dependent on the volume of mucilage produced, but the number was not, suggesting that proliferation of stacks is developmentally programmed. Mucilage-producing Golgi stacks were randomly distributed in the cell and not clustered at the site of secretion.
Santelia, Diana
- You have accessRestricted AccessThe Modified Flavonol Glycosylation Profile in the Arabidopsis rol1 Mutants Results in Alterations in Plant Growth and Cell Shape FormationChristoph Ringli, Laurent Bigler, Benjamin M. Kuhn, Ruth-Maria Leiber, Anouck Diet, Diana Santelia, Beat Frey, Stephan Pollmann, Markus KleinPlant Cell Jun 2008, 20 (6) 1470-1481; DOI: https://doi.org/10.1105/tpc.107.053249
Flavonoids have been shown to affect growth in a variety of plant species. This work demonstrates that changes in the Arabidopsis flavonol glycosylation profile can interfere with cell development, resulting in defects in cotyledon growth, pavement cell shape, stomatal development, and trichome formation.
Sawano, Yoriko
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Schmidt, Esther E.
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Schreuder, Mariëlle
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Seifert, Georg J.
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Seki, Motoaki
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
Shen, Hui
- You have accessRestricted AccessLight-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 from Arabidopsis Depend upon Its Direct Physical Interactions with Photoactivated PhytochromesHui Shen, Ling Zhu, Alicia Castillon, Manoj Majee, Bruce Downie, Enamul HuqPlant Cell Jun 2008, 20 (6) 1586-1602; DOI: https://doi.org/10.1105/tpc.108.060020
PHYTOCHROME INTERACTING FACTOR1 is rapidly phosphorylated, ubiquitinated, and degraded under red and far-red light. Overexpression of a light-stable truncated PIF1 showed a constitutive photomorphogenic phenotype in the dark, suggesting that light-induced proteolytic degradation of negative regulators (e.g. PIFs) might be sufficient to promote photomorphogenesis.
Shi, Dongqiao
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Shinozaki, Kazuo
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Smith, Alison
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Stein, Lincoln D.
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Sun, Kangtai
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
T
Tanokura, Masaru
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Tran, Lam-Son Phan
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Trelease, Richard N.
- You have accessRestricted AccessArabidopsis PEROXIN11c-e, FISSION1b, and DYNAMIN-RELATED PROTEIN3A Cooperate in Cell Cycle–Associated Replication of PeroxisomesMatthew J. Lingard, Satinder K. Gidda, Scott Bingham, Steven J. Rothstein, Robert T. Mullen, Richard N. TreleasePlant Cell Jun 2008, 20 (6) 1567-1585; DOI: https://doi.org/10.1105/tpc.107.057679
This study examines the molecular interactions of PEROXIN11 isoforms with each other, with FISSION1 isoforms, and with DYNAMIN-RELATED PROTEIN3A (DRP3A) in peroxisome replication during cell division. Gene silencing and protein interaction experiments show that these proteins play distinct but overlapping roles in the elongation and fission of replicating peroxisomes.
Truernit, Elisabeth
- You have accessRestricted AccessHigh-Resolution Whole-Mount Imaging of Three-Dimensional Tissue Organization and Gene Expression Enables the Study of Phloem Development and Structure in ArabidopsisElisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, Julien Barthélémy, Jean-Christophe PalauquiPlant Cell Jun 2008, 20 (6) 1494-1503; DOI: https://doi.org/10.1105/tpc.107.056069
This work images phloem development using a remarkable improvement in plant tissue staining that, in combination with confocal microscopy, reveals three-dimensional cellular organization of plant tissue with a precision that formerly required tissue sectioning. Furthermore, coupled with staining for β-glucuronidase activity, this method shows gene expression with single-cell resolution.
Tsesmetzis, Nicolas
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Tsuge, Tomohiko
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Tsunoda, Satoshi P.
- You have accessRestricted AccessChannelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-Induced DepolarizationPeter Berthold, Satoshi P. Tsunoda, Oliver P. Ernst, Wolfgang Mages, Dietrich Gradmann, Peter HegemannPlant Cell Jun 2008, 20 (6) 1665-1677; DOI: https://doi.org/10.1105/tpc.108.057919
This study reanalyzed channelrhodopsin-1 (CHR1)–based photocurrents in Chlamydomonas and demonstrated that, in addition to H+, ChR1 also conducts other cations. This conductance enables the alga to orient in the light even at alkaline pH.
Tsutsumi, Nobuhiro
- You have accessRestricted AccessArabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission SitesShin-ichi Arimura, Masaru Fujimoto, Yoko Doniwa, Naoki Kadoya, Mikio Nakazono, Wataru Sakamoto, Nobuhiro TsutsumiPlant Cell Jun 2008, 20 (6) 1555-1566; DOI: https://doi.org/10.1105/tpc.108.058578
Mitochondrial fission is a fundamental but poorly understood process in plant cells. DYNAMIN-RELATED PROTEIN3A is a conserved eukaryotic mitochondrial fission factor found in Arabidopsis that forms a ring around and severs mitochondria. Here, we identify a novel plant-specific factor, ELONGATED MITOCHONDRIA1, that is required for the correct localization of DRP3A.
U
Umeda, Masaaki
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Umezawa, Taishi
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
V
van der Krol, Sander
- You have accessRestricted AccessIdentification of the Gene Encoding the α1,3-Mannosyltransferase (ALG3) in Arabidopsis and Characterization of Downstream N-Glycan ProcessingMaurice Henquet, Ludwig Lehle, Mariëlle Schreuder, Gerard Rouwendal, Jos Molthoff, Johannes Helsper, Sander van der Krol, Dirk BoschPlant Cell Jun 2008, 20 (6) 1652-1664; DOI: https://doi.org/10.1105/tpc.108.060731
This work describes the identification of a plant glycosyltransferase that is involved in the initial phase of N-glycan biosynthesis in the endoplasmic reticulum. A detailed analysis of a mutant of this glycosyltransferase provides insights into properties of downstream enzymes in the N-glycan biosynthesis pathway in plants.
Vastrik, Imre
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Vega-Sánchez, Miguel E.
- You have accessRestricted AccessSPIN1, a K Homology Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11, Is Involved in Flowering Time Control in RiceMiguel E. Vega-Sánchez, Lirong Zeng, Songbiao Chen, Hei Leung, Guo-Liang WangPlant Cell Jun 2008, 20 (6) 1456-1469; DOI: https://doi.org/10.1105/tpc.108.058610
Both RNA processing and ubiquitination play important roles in the regulation of flowering. This work implicates the E3 ligase SPOTTED LEAF11 and its substrate SPIN1, a nuclear RNA/DNA binding protein of the STAR family, in rice flowering time control. This study links ubiquitination and RNA/DNA metabolism in flowering time regulation in plants.
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Walsh, Sean V.
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
Wang, Guo-Liang
- You have accessRestricted AccessSPIN1, a K Homology Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11, Is Involved in Flowering Time Control in RiceMiguel E. Vega-Sánchez, Lirong Zeng, Songbiao Chen, Hei Leung, Guo-Liang WangPlant Cell Jun 2008, 20 (6) 1456-1469; DOI: https://doi.org/10.1105/tpc.108.058610
Both RNA processing and ubiquitination play important roles in the regulation of flowering. This work implicates the E3 ligase SPOTTED LEAF11 and its substrate SPIN1, a nuclear RNA/DNA binding protein of the STAR family, in rice flowering time control. This study links ubiquitination and RNA/DNA metabolism in flowering time regulation in plants.
Wang, Jia
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
Western, Tamara L.
- Open AccessAnalysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich MucilageRobin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn, A. Lacey SamuelsPlant Cell Jun 2008, 20 (6) 1623-1638; DOI: https://doi.org/10.1105/tpc.108.058842
Golgi stacks in Arabidopsis seed coat cells produce mucilage synchronously at a specific stage of differentiation. The morphology of stacks was dependent on the volume of mucilage produced, but the number was not, suggesting that proliferation of stacks is developmentally programmed. Mucilage-producing Golgi stacks were randomly distributed in the cell and not clustered at the site of secretion.
Wu, Guanming
- You have accessRestricted AccessArabidopsis Reactome: A Foundation Knowledgebase for Plant Systems BiologyNicolas Tsesmetzis, Matthew Couchman, Janet Higgins, Alison Smith, John H. Doonan, Georg J. Seifert, Esther E. Schmidt, Imre Vastrik, Ewan Birney, Guanming Wu, Peter D'Eustachio, Lincoln D. Stein, Richard J. Morris, Michael W. Bevan, Sean V. WalshPlant Cell Jun 2008, 20 (6) 1426-1436; DOI: https://doi.org/10.1105/tpc.108.057976
X
Xie, Qi
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Xiong, Yue
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
Y
Yakoumakis, Dimitrios I.
- You have accessRestricted AccessSpermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in TobaccoPanagiotis N. Moschou, Konstantinos A. Paschalidis, Ioannis D. Delis, Athina H. Andriopoulou, George D. Lagiotis, Dimitrios I. Yakoumakis, Kalliopi A. Roubelakis-AngelakisPlant Cell Jun 2008, 20 (6) 1708-1724; DOI: https://doi.org/10.1105/tpc.108.059733
Polyamines play a protective role against stress challenges in plants. This work shows that abiotic stress induces spermidine secretion into the apoplast, where it is oxidized by polyamine oxidase. Depending on its levels and those of intracellular polyamines, the generated H2O2 induces either tolerance events or programmed cell death.
Yamaguchi-Shinozaki, Kazuko
- You have accessRestricted AccessArabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene ExpressionFeng Qin, Yoh Sakuma, Lam-Son Phan Tran, Kyonoshin Maruyama, Satoshi Kidokoro, Yasunari Fujita, Miki Fujita, Taishi Umezawa, Yoriko Sawano, Ken-ichi Miyazono, Masaru Tanokura, Kazuo Shinozaki, Kazuko Yamaguchi-ShinozakiPlant Cell Jun 2008, 20 (6) 1693-1707; DOI: https://doi.org/10.1105/tpc.107.057380
Drought-inducible gene expression is controlled by the key transcription factor DREB2A. We identified two C3HC4 RING domain–containing proteins that interact with DREB2A and function as E3 ubiquitin ligases to mediate DREB2A degradation to tightly control DREB2A protein abundance.
Yamauchi, Kazuchika
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Yang, Weicai
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Yasuda, Michiko
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
Yoshida, Shigeo
- You have accessRestricted AccessAntagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in ArabidopsisMichiko Yasuda, Atsushi Ishikawa, Yusuke Jikumaru, Motoaki Seki, Taishi Umezawa, Tadao Asami, Akiko Maruyama-Nakashita, Toshiaki Kudo, Kazuo Shinozaki, Shigeo Yoshida, Hideo NakashitaPlant Cell Jun 2008, 20 (6) 1678-1692; DOI: https://doi.org/10.1105/tpc.107.054296
Several types of self-protecting systems that involve hormone signaling allow plants to survive various types of external stresses. This work details antagonistic interactions between abscisic acid–mediated responses to environmental stresses and salicylic acid–mediated signaling in the induction of systemic acquired resistance to pathogens.
Young, Robin E.
- Open AccessAnalysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich MucilageRobin E. Young, Heather E. McFarlane, Michael G. Hahn, Tamara L. Western, George W. Haughn, A. Lacey SamuelsPlant Cell Jun 2008, 20 (6) 1623-1638; DOI: https://doi.org/10.1105/tpc.108.058842
Golgi stacks in Arabidopsis seed coat cells produce mucilage synchronously at a specific stage of differentiation. The morphology of stacks was dependent on the volume of mucilage produced, but the number was not, suggesting that proliferation of stacks is developmentally programmed. Mucilage-producing Golgi stacks were randomly distributed in the cell and not clustered at the site of secretion.
Z
Zabotina, Olga A.
- Open AccessDisrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall ComponentDavid M. Cavalier, Olivier Lerouxel, Lutz Neumetzler, Kazuchika Yamauchi, Antje Reinecke, Glenn Freshour, Olga A. Zabotina, Michael G. Hahn, Ingo Burgert, Markus Pauly, Natasha V. Raikhel, Kenneth KeegstraPlant Cell Jun 2008, 20 (6) 1519-1537; DOI: https://doi.org/10.1105/tpc.108.059873
This study adopted a reverse genetics approach to provide evidence that two Arabidopsis xylosyltransferase genes are involved in xyloglucan biosynthesis in vivo. Disrupting both of these genes resulted in plants with a severe root hair phenotype that lacked detectable xyloglucan. These findings challenge conventional models of the plant primary cell wall.
Zeng, Lirong
- You have accessRestricted AccessSPIN1, a K Homology Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11, Is Involved in Flowering Time Control in RiceMiguel E. Vega-Sánchez, Lirong Zeng, Songbiao Chen, Hei Leung, Guo-Liang WangPlant Cell Jun 2008, 20 (6) 1456-1469; DOI: https://doi.org/10.1105/tpc.108.058610
Both RNA processing and ubiquitination play important roles in the regulation of flowering. This work implicates the E3 ligase SPOTTED LEAF11 and its substrate SPIN1, a nuclear RNA/DNA binding protein of the STAR family, in rice flowering time control. This study links ubiquitination and RNA/DNA metabolism in flowering time regulation in plants.
Zhang, Yiyue
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Zhang, Yu
- You have accessRestricted AccessArabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 Forms a Nuclear E3 Ubiquitin Ligase with DDB1 and CUL4 That Is Involved in Multiple Plant Developmental ProcessesYu Zhang, Suhua Feng, Fangfang Chen, Haodong Chen, Jia Wang, Chad McCall, Yue Xiong, Xing Wang DengPlant Cell Jun 2008, 20 (6) 1437-1455; DOI: https://doi.org/10.1105/tpc.108.058891
Protein ubiquitination is a key posttranslational modification in eukaryotes. This study reveals a nuclear CULLIN4-based E3 ubiquitin ligase complex in Arabidopsis that plays a role in numerous plant developmental processes.
Zhao, Qiao
- You have accessRestricted AccessTwo Distinct Interacting Classes of Nuclear Envelope–Associated Coiled-Coil Proteins Are Required for the Tissue-Specific Nuclear Envelope Targeting of Arabidopsis RanGAPQiao Zhao, Jelena Brkljacic, Iris MeierPlant Cell Jun 2008, 20 (6) 1639-1651; DOI: https://doi.org/10.1105/tpc.108.059220
Tandem affinity purification and mass spectrometry were used to identify a family of novel coiled-coil proteins required for nuclear envelope association of RanGAP1 in Arabidopsis root tip cells. The findings suggest an unanticipated complexity of RanGAP nuclear envelope targeting in higher plant cells, involving at least two families of likely plant-specific nuclear pore–associated proteins.
Zheng, Nuoyan
- Open AccessTargeted Degradation of the Cyclin-Dependent Kinase Inhibitor ICK4/KRP6 by RING-Type E3 Ligases Is Essential for Mitotic Cell Cycle Progression during Arabidopsis GametogenesisJingjing Liu, Yiyue Zhang, Genji Qin, Tomohiko Tsuge, Norihiro Sakaguchi, Guo Luo, Kangtai Sun, Dongqiao Shi, Shiori Aki, Nuoyan Zheng, Takashi Aoyama, Atsuhiro Oka, Weicai Yang, Masaaki Umeda, Qi Xie, Hongya Gu, Li-Jia QuPlant Cell Jun 2008, 20 (6) 1538-1554; DOI: https://doi.org/10.1105/tpc.108.059741
This work uses genetic and biochemical assays to examine two RING-type E3 ligases that interact with and target a specific inhibitor of cyclin-dependent kinases, ICK4/KRP6, for proteasome-mediated degradation. This cell cycle inhibitor is expressed during gametophyte meiosis and must be degraded prior to the subsequent mitotic divisions that form the male and female gametophytes.
Zhu, Ling
- You have accessRestricted AccessLight-Induced Phosphorylation and Degradation of the Negative Regulator PHYTOCHROME-INTERACTING FACTOR1 from Arabidopsis Depend upon Its Direct Physical Interactions with Photoactivated PhytochromesHui Shen, Ling Zhu, Alicia Castillon, Manoj Majee, Bruce Downie, Enamul HuqPlant Cell Jun 2008, 20 (6) 1586-1602; DOI: https://doi.org/10.1105/tpc.108.060020
PHYTOCHROME INTERACTING FACTOR1 is rapidly phosphorylated, ubiquitinated, and degraded under red and far-red light. Overexpression of a light-stable truncated PIF1 showed a constitutive photomorphogenic phenotype in the dark, suggesting that light-induced proteolytic degradation of negative regulators (e.g. PIFs) might be sufficient to promote photomorphogenesis.
Zhu, Yu-Xian
- Open AccessMutation of Arabidopsis BARD1 Causes Meristem Defects by Failing to Confine WUSCHEL Expression to the Organizing CenterPei Han, Qing Li, Yu-Xian ZhuPlant Cell Jun 2008, 20 (6) 1482-1493; DOI: https://doi.org/10.1105/tpc.108.058867
BARD1 represses the transcriptional activity of WUSCHEL, a gene essential for maintenance of stem cell fate in Arabidopsis. In a bard1-null mutant, severe shoot apical meristem defects are observed as a result of WUSCHEL expression outside of the meristem organizing center. BARD1 may inhibit a chromatin-remodeling process vital for WUS expression.