Table of Contents

This work shows that SOC1 transcripts are directly recognized and regulated by an RNA binding protein EARLY FLOWERING9 (ELF9). In addition, ELF9 regulates multiple transcripts that are the targets of nonsense-mediated mRNA decay (NMD). Thus, ELF9 might be an RNA binding component of the Arabidopsis NMD system.

For plant infection, the cucumber anthracnose fungus requires Atg26, a glucosyl transferase involved in pexophagy, the selective degradation of peroxisomes. Appressoria of an atg26 mutant fail to develop invasion hyphae and show defective pexophagy. Domain analysis of Atg26 suggests that both its phosphoinositide binding domain and catalytic domain are required for pexophagy and pathogenicity.

Little is known about how cellulose synthases (CESAs) reach their site of activity at the plasma membrane. Here, intracellular trafficking of CESA3 was investigated using live-cell imaging and electron microscopy. CESA3 secretion is accompanied by pausing of Golgi bodies on microtubules, and CESA3 is internalized in compartments whose movement depends on cortical microtubule array dynamics.

This work shows that the plant pathogen Xanthomonas campestris pathovar vesicatoria uses the XopN T3S effector protein to suppress tomato defense responses at the early stages of pathogen recognition. Biochemical studies indicate that XopN interacts specifically with a tomato atypical receptor kinase TARK1 that is partially required to limit bacterial multiplication in leaves.

The authors analyze host and pathogen gene expression after enrichment for biotrophic invasive hyphae (IH) of Magnaporthe oryzae growing in the first-invaded rice cells. IH express genes encoding biotrophy-associated secreted proteins, some of which accumulate in blast biotrophic interfacial complexes in compatible interactions, resemble known avirulence effectors.

This study uses an RNAi screen in Zinnia to identify two new components, Tracheary Element Differentiation-Related6 (TED6) and TED7, which contribute to cell wall biosynthesis during xylem vessel formation. The authors study TED6 and TED7 functions and interactions in Arabidopsis plants and Zinnia cultured cells.

In this work, expression of a fatty acid elongase in the epidermis results in glabrous plants because newly initiated trichomes undergo rapid cell death. Chemical genetic screens identify cell death inhibitors that restore cell differentiation program.

In this work, expression of a fatty acid elongase in the epidermis results in glabrous plants because newly initiated trichomes undergo rapid cell death. Chemical genetic screens identify cell death inhibitors that restore cell differentiation program.

In this work, expression of a fatty acid elongase in the epidermis results in glabrous plants because newly initiated trichomes undergo rapid cell death. Chemical genetic screens identify cell death inhibitors that restore cell differentiation program.

Little is known about how cellulose synthases (CESAs) reach their site of activity at the plasma membrane. Here, intracellular trafficking of CESA3 was investigated using live-cell imaging and electron microscopy. CESA3 secretion is accompanied by pausing of Golgi bodies on microtubules, and CESA3 is internalized in compartments whose movement depends on cortical microtubule array dynamics.

In this work, expression of a fatty acid elongase in the epidermis results in glabrous plants because newly initiated trichomes undergo rapid cell death. Chemical genetic screens identify cell death inhibitors that restore cell differentiation program.

This work examines the role of the late wound-response gene Leucine aminopeptidase A (LapA) in herbivory and wound-induced gene expression. Examination of silenced and overexpressing lines indicates that LAP-A promotes defense responses downstream of jasmonic acid.

The authors analyze host and pathogen gene expression after enrichment for biotrophic invasive hyphae (IH) of Magnaporthe oryzae growing in the first-invaded rice cells. IH express genes encoding biotrophy-associated secreted proteins, some of which accumulate in blast biotrophic interfacial complexes in compatible interactions, resemble known avirulence effectors.

This work shows that the plant pathogen Xanthomonas campestris pathovar vesicatoria uses the XopN T3S effector protein to suppress tomato defense responses at the early stages of pathogen recognition. Biochemical studies indicate that XopN interacts specifically with a tomato atypical receptor kinase TARK1 that is partially required to limit bacterial multiplication in leaves.

Analysis of post-Golgi transport machinery revealed a mobile structure of clustered secretory vesicles, termed the secretory vesicle cluster (SVC), which originates from the trans-Golgi network and fuses with plasma membrane or cell plate. The presence of secretory proteins and glycans suggested that SVC is involved in mass transport from the Golgi to the cell exterior.

The Arabidopsis GCN5 histone acetyltransferase complex attenuates the recently discovered gradient expression of the PLETHORA genes and thereby regulates root stem cell niche maintenance and proliferation. Therefore, chromatin modifications play an important role in stem cell maintenance and in shaping a developmentally instructive gradient in the root.

It has long been hypothesized that extracellular transfer of lipids across the epidermal cell wall to the waxy cuticle would require lipid transfer proteins (LTPs). An LTP with a GPI-anchor, LTPG, is required for export of wax to the cuticle in the stems of Arabidopsis. LTPG is primarily localized to the plasma membrane, and recombinant LTPG displays binding affinity for a lipophilic analog.

This work studies the role of a homolog of bacterial recombinase RecA in the dynamics of the plant mitochondrial genome. The analysis of mitochondrial recA disruptants shows that RecA does not promote but rather suppresses recombination among short repeated sequences scattered throughout the mitochondrial genome, thereby maintaining mitochondrial genome stability.

GOLDEN2-LIKE (GLK) transcription factors are required for normal chloroplast development in several land plants. This study shows that GLK proteins synchronously upregulate genes relating to light harvesting and chlorophyll biosynthesis via direct interaction with promoter sequences. GLK activity most likely optimizes photosynthesis by coordinating responses to variable environmental conditions.

Higher plant cells organize microtubules in the absence of a structurally defined microtubule-organizing center. This article reports that the WD40 repeat protein NEDD1 appears preferentially toward the minus ends of microtubules and plays a critical role in microtubule organization during cell division in Arabidopsis thaliana.

This work shows that the plant pathogen Xanthomonas campestris pathovar vesicatoria uses the XopN T3S effector protein to suppress tomato defense responses at the early stages of pathogen recognition. Biochemical studies indicate that XopN interacts specifically with a tomato atypical receptor kinase TARK1 that is partially required to limit bacterial multiplication in leaves.

Higher plant cells organize microtubules in the absence of a structurally defined microtubule-organizing center. This article reports that the WD40 repeat protein NEDD1 appears preferentially toward the minus ends of microtubules and plays a critical role in microtubule organization during cell division in Arabidopsis thaliana.

Analysis of post-Golgi transport machinery revealed a mobile structure of clustered secretory vesicles, termed the secretory vesicle cluster (SVC), which originates from the trans-Golgi network and fuses with plasma membrane or cell plate. The presence of secretory proteins and glycans suggested that SVC is involved in mass transport from the Golgi to the cell exterior.

In this work, expression of a fatty acid elongase in the epidermis results in glabrous plants because newly initiated trichomes undergo rapid cell death. Chemical genetic screens identify cell death inhibitors that restore cell differentiation program.

The authors analyze host and pathogen gene expression after enrichment for biotrophic invasive hyphae (IH) of Magnaporthe oryzae growing in the first-invaded rice cells. IH express genes encoding biotrophy-associated secreted proteins, some of which accumulate in blast biotrophic interfacial complexes in compatible interactions, resemble known avirulence effectors.

In this work, expression of a fatty acid elongase in the epidermis results in glabrous plants because newly initiated trichomes undergo rapid cell death. Chemical genetic screens identify cell death inhibitors that restore cell differentiation program.

For plant infection, the cucumber anthracnose fungus requires Atg26, a glucosyl transferase involved in pexophagy, the selective degradation of peroxisomes. Appressoria of an atg26 mutant fail to develop invasion hyphae and show defective pexophagy. Domain analysis of Atg26 suggests that both its phosphoinositide binding domain and catalytic domain are required for pexophagy and pathogenicity.

This study uses an RNAi screen in Zinnia to identify two new components, Tracheary Element Differentiation-Related6 (TED6) and TED7, which contribute to cell wall biosynthesis during xylem vessel formation. The authors study TED6 and TED7 functions and interactions in Arabidopsis plants and Zinnia cultured cells.

The first step of shoot branching is production of axillary meristems (AMs) that are formed in the axils of leaves. Rice LAX PANICLE1 (LAX1) is required for AM formation through the plant's life. Here, we show that LAX1 protein, produced in the boundary between the subtending leaf and the future AM, moves toward the growing AM to establish a new AM.

This genome-wide survey shows that epigenetic patterns differ significantly between protein-coding genes and transposable elements in maize. It is found that activating histone modifications co-occur, but the repressive marks H3K27me3 and DNA methylation exclude each other. Evidence is provided that changing mop1 transcript levels might lead to a tissue-specific shift of small RNA populations.

Little is known about how cellulose synthases (CESAs) reach their site of activity at the plasma membrane. Here, intracellular trafficking of CESA3 was investigated using live-cell imaging and electron microscopy. CESA3 secretion is accompanied by pausing of Golgi bodies on microtubules, and CESA3 is internalized in compartments whose movement depends on cortical microtubule array dynamics.

It has long been hypothesized that extracellular transfer of lipids across the epidermal cell wall to the waxy cuticle would require lipid transfer proteins (LTPs). An LTP with a GPI-anchor, LTPG, is required for export of wax to the cuticle in the stems of Arabidopsis. LTPG is primarily localized to the plasma membrane, and recombinant LTPG displays binding affinity for a lipophilic analog.

GOLDEN2-LIKE (GLK) transcription factors are required for normal chloroplast development in several land plants. This study shows that GLK proteins synchronously upregulate genes relating to light harvesting and chlorophyll biosynthesis via direct interaction with promoter sequences. GLK activity most likely optimizes photosynthesis by coordinating responses to variable environmental conditions.

The precise specification of distinct cell fates during development requires accurate regulatory mechanisms. This work shows that MYB23 provides a positive transcriptional feedback loop for cell fate specification in the Arabidopsis root epidermis, and together with the previously identified lateral inhibition mechanism, ensures robust establishment of the cell type pattern.

Little is known about how cellulose synthases (CESAs) reach their site of activity at the plasma membrane. Here, intracellular trafficking of CESA3 was investigated using live-cell imaging and electron microscopy. CESA3 secretion is accompanied by pausing of Golgi bodies on microtubules, and CESA3 is internalized in compartments whose movement depends on cortical microtubule array dynamics.

This work shows that SOC1 transcripts are directly recognized and regulated by an RNA binding protein EARLY FLOWERING9 (ELF9). In addition, ELF9 regulates multiple transcripts that are the targets of nonsense-mediated mRNA decay (NMD). Thus, ELF9 might be an RNA binding component of the Arabidopsis NMD system.

Little is known about how cellulose synthases (CESAs) reach their site of activity at the plasma membrane. Here, intracellular trafficking of CESA3 was investigated using live-cell imaging and electron microscopy. CESA3 secretion is accompanied by pausing of Golgi bodies on microtubules, and CESA3 is internalized in compartments whose movement depends on cortical microtubule array dynamics.

For plant infection, the cucumber anthracnose fungus requires Atg26, a glucosyl transferase involved in pexophagy, the selective degradation of peroxisomes. Appressoria of an atg26 mutant fail to develop invasion hyphae and show defective pexophagy. Domain analysis of Atg26 suggests that both its phosphoinositide binding domain and catalytic domain are required for pexophagy and pathogenicity.

This study uses an RNAi screen in Zinnia to identify two new components, Tracheary Element Differentiation-Related6 (TED6) and TED7, which contribute to cell wall biosynthesis during xylem vessel formation. The authors study TED6 and TED7 functions and interactions in Arabidopsis plants and Zinnia cultured cells.

Little is known about how cellulose synthases (CESAs) reach their site of activity at the plasma membrane. Here, intracellular trafficking of CESA3 was investigated using live-cell imaging and electron microscopy. CESA3 secretion is accompanied by pausing of Golgi bodies on microtubules, and CESA3 is internalized in compartments whose movement depends on cortical microtubule array dynamics.

GOLDEN2-LIKE (GLK) transcription factors are required for normal chloroplast development in several land plants. This study shows that GLK proteins synchronously upregulate genes relating to light harvesting and chlorophyll biosynthesis via direct interaction with promoter sequences. GLK activity most likely optimizes photosynthesis by coordinating responses to variable environmental conditions.

For plant infection, the cucumber anthracnose fungus requires Atg26, a glucosyl transferase involved in pexophagy, the selective degradation of peroxisomes. Appressoria of an atg26 mutant fail to develop invasion hyphae and show defective pexophagy. Domain analysis of Atg26 suggests that both its phosphoinositide binding domain and catalytic domain are required for pexophagy and pathogenicity.