Table of Contents
A
Ahn, Tae Kyu
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
Alonso, Rosario
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
Aparna, Gudlur
- You have accessRestricted AccessA Cell Wall–Degrading Esterase of Xanthomonas oryzae Requires a Unique Substrate Recognition Module for Pathogenesis on RiceGudlur Aparna, Avradip Chatterjee, Ramesh V. Sonti, Rajan SankaranarayananPlant Cell Jun 2009, 21 (6) 1860-1873; DOI: https://doi.org/10.1105/tpc.109.066886
The structural study of a rice cell wall–degrading esterase LipA from Xanthomonas oryzae pv oryzae, the causal agent of bacterial leaf blight disease, reveals a unique domain that is essential for LipA function in planta and is conserved across the phytopathogenic genus Xanthomonas.
Avenson, Thomas J.
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
B
Ballottari, Matteo
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
Bandyopadhyay, Anindita
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Bangari, Bharat
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Bassi, Roberto
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
Benfey, Philip N.
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Blakeslee, Joshua J.
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Bolduc, Nathalie
- Open AccessThe Maize Transcription Factor KNOTTED1 Directly Regulates the Gibberellin Catabolism Gene ga2ox1Nathalie Bolduc, Sarah HakePlant Cell Jun 2009, 21 (6) 1647-1658; DOI: https://doi.org/10.1105/tpc.109.068221
Little is known about how KN1 and other KNOX proteins promote the establishment and maintenance of plant meristems, as few of their targets have been identified. This work shows that KN1 negatively regulates gibberellin accumulation through positive control of ga2ox1, which codes for an enzyme that inactivates gibberellin. KN1 directly binds to a cis-regulatory element in an intron of ga2ox1.
Böttcher, Christoph
- Open AccessThe Multifunctional Enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) Converts Cysteine-Indole-3-Acetonitrile to Camalexin in the Indole-3-Acetonitrile Metabolic Network of Arabidopsis thalianaChristoph Böttcher, Lore Westphal, Constanze Schmotz, Elke Prade, Dierk Scheel, Erich GlawischnigPlant Cell Jun 2009, 21 (6) 1830-1845; DOI: https://doi.org/10.1105/tpc.109.066670
In a comprehensive metabolomics and precursor feeding approach, a network of indole-3-acetonitrile (IAN)–derived metabolites was identified in mutants of camalexin biosynthesis. A Cys conjugate of IAN was shown to be turned over directly to camalexin and cyanide by PHYTOALEXIN DEFICIENT3, a multifunctional cytochrome P450 enzyme.
Brodersen, Peter
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
C
Cang, Huaixing
- You have accessRestricted AccessCrystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-PtoJing Dong, Fangming Xiao, Fenxia Fan, Lichuan Gu, Huaixing Cang, Gregory B. Martin, Jijie ChaiPlant Cell Jun 2009, 21 (6) 1846-1859; DOI: https://doi.org/10.1105/tpc.109.066878
Resistance to bacterial speck disease in tomato is activated upon interaction of the host Pto kinase with either of two pathogen proteins. This work provides structural insight into how a single host resistance protein can recognize two divergent bacterial effector proteins and supports a proposed mechanism in which each of these effector proteins relieves negative regulation by Pto.
Carrie, Chris
- You have accessRestricted AccessExploring the Function-Location Nexus: Using Multiple Lines of Evidence in Defining the Subcellular Location of Plant ProteinsA. Harvey Millar, Chris Carrie, Barry Pogson, James WhelanPlant Cell Jun 2009, 21 (6) 1625-1631; DOI: https://doi.org/10.1105/tpc.109.066019
Chai, Jijie
- You have accessRestricted AccessCrystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-PtoJing Dong, Fangming Xiao, Fenxia Fan, Lichuan Gu, Huaixing Cang, Gregory B. Martin, Jijie ChaiPlant Cell Jun 2009, 21 (6) 1846-1859; DOI: https://doi.org/10.1105/tpc.109.066878
Resistance to bacterial speck disease in tomato is activated upon interaction of the host Pto kinase with either of two pathogen proteins. This work provides structural insight into how a single host resistance protein can recognize two divergent bacterial effector proteins and supports a proposed mechanism in which each of these effector proteins relieves negative regulation by Pto.
Chatterjee, Avradip
- You have accessRestricted AccessA Cell Wall–Degrading Esterase of Xanthomonas oryzae Requires a Unique Substrate Recognition Module for Pathogenesis on RiceGudlur Aparna, Avradip Chatterjee, Ramesh V. Sonti, Rajan SankaranarayananPlant Cell Jun 2009, 21 (6) 1860-1873; DOI: https://doi.org/10.1105/tpc.109.066886
The structural study of a rice cell wall–degrading esterase LipA from Xanthomonas oryzae pv oryzae, the causal agent of bacterial leaf blight disease, reveals a unique domain that is essential for LipA function in planta and is conserved across the phytopathogenic genus Xanthomonas.
Cheng, Yan
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Crété, Patrice
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
Cruz, Jeffrey A.
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
D
Delannoy, Etienne
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
Diaz, Isabel
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
Dietrich, Katrin
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
Dong, Jing
- You have accessRestricted AccessCrystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-PtoJing Dong, Fangming Xiao, Fenxia Fan, Lichuan Gu, Huaixing Cang, Gregory B. Martin, Jijie ChaiPlant Cell Jun 2009, 21 (6) 1846-1859; DOI: https://doi.org/10.1105/tpc.109.066878
Resistance to bacterial speck disease in tomato is activated upon interaction of the host Pto kinase with either of two pathogen proteins. This work provides structural insight into how a single host resistance protein can recognize two divergent bacterial effector proteins and supports a proposed mechanism in which each of these effector proteins relieves negative regulation by Pto.
Dröge-Laser, Wolfgang
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
E
Eckardt, Nancy A.
- You have accessRestricted AccessA High-Resolution Map of Auxin Distribution in the Arabidopsis Root ApexNancy A. EckardtPlant Cell Jun 2009, 21 (6) 1621; DOI: https://doi.org/10.1105/tpc.109.210610
- You have accessRestricted AccessInvestigating Translational Repression by MicroRNAs in ArabidopsisNancy A. EckardtPlant Cell Jun 2009, 21 (6) 1624; DOI: https://doi.org/10.1105/tpc.109.210613
Ehlert, Andrea
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
F
Fan, Fenxia
- You have accessRestricted AccessCrystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-PtoJing Dong, Fangming Xiao, Fenxia Fan, Lichuan Gu, Huaixing Cang, Gregory B. Martin, Jijie ChaiPlant Cell Jun 2009, 21 (6) 1846-1859; DOI: https://doi.org/10.1105/tpc.109.066878
Resistance to bacterial speck disease in tomato is activated upon interaction of the host Pto kinase with either of two pathogen proteins. This work provides structural insight into how a single host resistance protein can recognize two divergent bacterial effector proteins and supports a proposed mechanism in which each of these effector proteins relieves negative regulation by Pto.
Farquharson, Kathleen L.
- You have accessRestricted AccessA Plastidic Transporter Involved in Aliphatic Glucosinolate BiosynthesisKathleen L. FarquharsonPlant Cell Jun 2009, 21 (6) 1622; DOI: https://doi.org/10.1105/tpc.109.210611
Fleming, Graham R.
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
Floris, Maïna
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
Flügge, Ulf-Ingo
- You have accessRestricted AccessThe Plastidic Bile Acid Transporter 5 Is Required for the Biosynthesis of Methionine-Derived Glucosinolates in Arabidopsis thalianaTamara Gigolashvili, Ruslan Yatusevich, Inga Rollwitz, Melanie Humphry, Jonathan Gershenzon, Ulf-Ingo FlüggePlant Cell Jun 2009, 21 (6) 1813-1829; DOI: https://doi.org/10.1105/tpc.109.066399
This work demonstrates that the chloroplastidic BAT5 transporter is required for side chain elongation of Met-derived glucosinolates in Arabidopsis. Comparative feeding experiments of bat5 and wild-type plants using 2-keto acids and amino acids of different chain length suggested that BAT5 functions as a plastidic transporter for 2-keto acids.
G
Gershenzon, Jonathan
- You have accessRestricted AccessThe Plastidic Bile Acid Transporter 5 Is Required for the Biosynthesis of Methionine-Derived Glucosinolates in Arabidopsis thalianaTamara Gigolashvili, Ruslan Yatusevich, Inga Rollwitz, Melanie Humphry, Jonathan Gershenzon, Ulf-Ingo FlüggePlant Cell Jun 2009, 21 (6) 1813-1829; DOI: https://doi.org/10.1105/tpc.109.066399
This work demonstrates that the chloroplastidic BAT5 transporter is required for side chain elongation of Met-derived glucosinolates in Arabidopsis. Comparative feeding experiments of bat5 and wild-type plants using 2-keto acids and amino acids of different chain length suggested that BAT5 functions as a plastidic transporter for 2-keto acids.
Gigolashvili, Tamara
- You have accessRestricted AccessThe Plastidic Bile Acid Transporter 5 Is Required for the Biosynthesis of Methionine-Derived Glucosinolates in Arabidopsis thalianaTamara Gigolashvili, Ruslan Yatusevich, Inga Rollwitz, Melanie Humphry, Jonathan Gershenzon, Ulf-Ingo FlüggePlant Cell Jun 2009, 21 (6) 1813-1829; DOI: https://doi.org/10.1105/tpc.109.066399
This work demonstrates that the chloroplastidic BAT5 transporter is required for side chain elongation of Met-derived glucosinolates in Arabidopsis. Comparative feeding experiments of bat5 and wild-type plants using 2-keto acids and amino acids of different chain length suggested that BAT5 functions as a plastidic transporter for 2-keto acids.
Glawischnig, Erich
- Open AccessThe Multifunctional Enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) Converts Cysteine-Indole-3-Acetonitrile to Camalexin in the Indole-3-Acetonitrile Metabolic Network of Arabidopsis thalianaChristoph Böttcher, Lore Westphal, Constanze Schmotz, Elke Prade, Dierk Scheel, Erich GlawischnigPlant Cell Jun 2009, 21 (6) 1830-1845; DOI: https://doi.org/10.1105/tpc.109.066670
In a comprehensive metabolomics and precursor feeding approach, a network of indole-3-acetonitrile (IAN)–derived metabolites was identified in mutants of camalexin biosynthesis. A Cys conjugate of IAN was shown to be turned over directly to camalexin and cyanide by PHYTOALEXIN DEFICIENT3, a multifunctional cytochrome P450 enzyme.
Grebe, Markus
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Gu, Lichuan
- You have accessRestricted AccessCrystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-PtoJing Dong, Fangming Xiao, Fenxia Fan, Lichuan Gu, Huaixing Cang, Gregory B. Martin, Jijie ChaiPlant Cell Jun 2009, 21 (6) 1846-1859; DOI: https://doi.org/10.1105/tpc.109.066878
Resistance to bacterial speck disease in tomato is activated upon interaction of the host Pto kinase with either of two pathogen proteins. This work provides structural insight into how a single host resistance protein can recognize two divergent bacterial effector proteins and supports a proposed mechanism in which each of these effector proteins relieves negative regulation by Pto.
Gu, Xiaofeng
- You have accessRestricted AccessEstablishment of the Winter-Annual Growth Habit via FRIGIDA-Mediated Histone Methylation at FLOWERING LOCUS C in ArabidopsisDanhua Jiang, Xiaofeng Gu, Yuehui HePlant Cell Jun 2009, 21 (6) 1733-1746; DOI: https://doi.org/10.1105/tpc.109.067967
This study shows that an Arabidopsis homolog of the human WDR5 protein, a core component of histone H3 lysine-4 (H3K4) methyltransferase complexes, binds the H3 tail and interacts with an H3K4 methyltransferase. FRIGIDA mediates the enrichment of this protein at FLOWERING LOCUS C, leading to elevated H3K4 trimethylation in FLC, to upregulate its expression and thus inhibits flowering.
H
Hake, Sarah
- Open AccessThe Maize Transcription Factor KNOTTED1 Directly Regulates the Gibberellin Catabolism Gene ga2ox1Nathalie Bolduc, Sarah HakePlant Cell Jun 2009, 21 (6) 1647-1658; DOI: https://doi.org/10.1105/tpc.109.068221
Little is known about how KN1 and other KNOX proteins promote the establishment and maintenance of plant meristems, as few of their targets have been identified. This work shows that KN1 negatively regulates gibberellin accumulation through positive control of ga2ox1, which codes for an enzyme that inactivates gibberellin. KN1 directly binds to a cis-regulatory element in an intron of ga2ox1.
Hall, Anthony
- You have accessRestricted AccessA Role for Multiple Circadian Clock Genes in the Response to Signals That Break Seed Dormancy in ArabidopsisSteven Penfield, Anthony HallPlant Cell Jun 2009, 21 (6) 1722-1732; DOI: https://doi.org/10.1105/tpc.108.064022
After-ripening is a little-known process occurring in dry seeds that results in loss of dormancy upon imbibition. This work shows that after-ripening affects the expression of genes comprising the circadian oscillator and that well-known circadian clock genes have roles in the control of Arabidopsis seed germination.
He, Yuehui
- You have accessRestricted AccessEstablishment of the Winter-Annual Growth Habit via FRIGIDA-Mediated Histone Methylation at FLOWERING LOCUS C in ArabidopsisDanhua Jiang, Xiaofeng Gu, Yuehui HePlant Cell Jun 2009, 21 (6) 1733-1746; DOI: https://doi.org/10.1105/tpc.109.067967
This study shows that an Arabidopsis homolog of the human WDR5 protein, a core component of histone H3 lysine-4 (H3K4) methyltransferase complexes, binds the H3 tail and interacts with an H3K4 methyltransferase. FRIGIDA mediates the enrichment of this protein at FLOWERING LOCUS C, leading to elevated H3K4 trimethylation in FLC, to upregulate its expression and thus inhibits flowering.
Hirabayashi, Yoshino
- You have accessRestricted AccessA 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope MembraneShingo Kikuchi, Maya Oishi, Yoshino Hirabayashi, Dong Wook Lee, Inhwan Hwang, Masato NakaiPlant Cell Jun 2009, 21 (6) 1781-1797; DOI: https://doi.org/10.1105/tpc.108.063552
This work describes the identification of a 1-MD translocation complex as a novel intermediate during general protein translocation across the inner membrane of chloroplasts. Tic20 and Tic21 are involved in the 1-MD complex, whereas Tic110, the most characterized component of the protein translocon at the inner membrane, exists as a distinct entity from the 1-MD complex.
Hofmann, Nancy R.
- You have accessRestricted AccessThe Tomato Pto Kinase Uses Shared and Unique Surfaces to Recognize Divergent Avirulence ProteinsNancy R. HofmannPlant Cell Jun 2009, 21 (6) 1623; DOI: https://doi.org/10.1105/tpc.109.210612
Hosein, Fazeeda N.
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Humphry, Melanie
- You have accessRestricted AccessThe Plastidic Bile Acid Transporter 5 Is Required for the Biosynthesis of Methionine-Derived Glucosinolates in Arabidopsis thalianaTamara Gigolashvili, Ruslan Yatusevich, Inga Rollwitz, Melanie Humphry, Jonathan Gershenzon, Ulf-Ingo FlüggePlant Cell Jun 2009, 21 (6) 1813-1829; DOI: https://doi.org/10.1105/tpc.109.066399
This work demonstrates that the chloroplastidic BAT5 transporter is required for side chain elongation of Met-derived glucosinolates in Arabidopsis. Comparative feeding experiments of bat5 and wild-type plants using 2-keto acids and amino acids of different chain length suggested that BAT5 functions as a plastidic transporter for 2-keto acids.
Hwang, Inhwan
- You have accessRestricted AccessA 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope MembraneShingo Kikuchi, Maya Oishi, Yoshino Hirabayashi, Dong Wook Lee, Inhwan Hwang, Masato NakaiPlant Cell Jun 2009, 21 (6) 1781-1797; DOI: https://doi.org/10.1105/tpc.108.063552
This work describes the identification of a 1-MD translocation complex as a novel intermediate during general protein translocation across the inner membrane of chloroplasts. Tic20 and Tic21 are involved in the 1-MD complex, whereas Tic110, the most characterized component of the protein translocon at the inner membrane, exists as a distinct entity from the 1-MD complex.
I
Ichikawa, Takanari
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
J
Jiang, Danhua
- You have accessRestricted AccessEstablishment of the Winter-Annual Growth Habit via FRIGIDA-Mediated Histone Methylation at FLOWERING LOCUS C in ArabidopsisDanhua Jiang, Xiaofeng Gu, Yuehui HePlant Cell Jun 2009, 21 (6) 1733-1746; DOI: https://doi.org/10.1105/tpc.109.067967
This study shows that an Arabidopsis homolog of the human WDR5 protein, a core component of histone H3 lysine-4 (H3K4) methyltransferase complexes, binds the H3 tail and interacts with an H3K4 methyltransferase. FRIGIDA mediates the enrichment of this protein at FLOWERING LOCUS C, leading to elevated H3K4 trimethylation in FLC, to upregulate its expression and thus inhibits flowering.
Johansson, Annika I.
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
K
Kabeya, Yukihiro
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
Keasling, Jay D.
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
Kikuchi, Shingo
- You have accessRestricted AccessA 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope MembraneShingo Kikuchi, Maya Oishi, Yoshino Hirabayashi, Dong Wook Lee, Inhwan Hwang, Masato NakaiPlant Cell Jun 2009, 21 (6) 1781-1797; DOI: https://doi.org/10.1105/tpc.108.063552
This work describes the identification of a 1-MD translocation complex as a novel intermediate during general protein translocation across the inner membrane of chloroplasts. Tic20 and Tic21 are involved in the 1-MD complex, whereas Tic110, the most characterized component of the protein translocon at the inner membrane, exists as a distinct entity from the 1-MD complex.
Kim, Jitae
- You have accessRestricted AccessSubunits of the Plastid ClpPR Protease Complex Have Differential Contributions to Embryogenesis, Plastid Biogenesis, and Plant Development in ArabidopsisJitae Kim, Andrea Rudella, Verenice Ramirez Rodriguez, Boris Zybailov, Paul Dominic B. Olinares, Klaas J. van WijkPlant Cell Jun 2009, 21 (6) 1669-1692; DOI: https://doi.org/10.1105/tpc.108.063784
Mutant, microscopy, and large-scale comparative proteome analyses of Arabidopsis demonstrate a central role of the Clp protease complex in chloroplast biogenesis and protein homeostasis, with different contributions of the ClpP and ClpR subunits. Lack of transcriptional and translational regulation suggests that regulation of Clp activity occurs through Clp complex assembly and substrate delivery.
Kliebenstein, DanielJ.
- You have accessRestricted AccessAdvancing Genetic Theory and Application by Metabolic Quantitative Trait Loci AnalysisDanielJ. KliebensteinPlant Cell Jun 2009, 21 (6) 1637-1646; DOI: https://doi.org/10.1105/tpc.109.067611
Kowalczyk, Mariusz
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Kramer, David M.
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
L
Lanet, Elodie
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
Lee, Dong Wook
- You have accessRestricted AccessA 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope MembraneShingo Kikuchi, Maya Oishi, Yoshino Hirabayashi, Dong Wook Lee, Inhwan Hwang, Masato NakaiPlant Cell Jun 2009, 21 (6) 1781-1797; DOI: https://doi.org/10.1105/tpc.108.063552
This work describes the identification of a 1-MD translocation complex as a novel intermediate during general protein translocation across the inner membrane of chloroplasts. Tic20 and Tic21 are involved in the 1-MD complex, whereas Tic110, the most characterized component of the protein translocon at the inner membrane, exists as a distinct entity from the 1-MD complex.
Lee, Gil-Je
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Li, Zhirong
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
Ljung, Karin
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
M
Makam, Srinivas N.
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Makoveychuk, Alexander
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Martin, Gregory B.
- You have accessRestricted AccessCrystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-PtoJing Dong, Fangming Xiao, Fenxia Fan, Lichuan Gu, Huaixing Cang, Gregory B. Martin, Jijie ChaiPlant Cell Jun 2009, 21 (6) 1846-1859; DOI: https://doi.org/10.1105/tpc.109.066878
Resistance to bacterial speck disease in tomato is activated upon interaction of the host Pto kinase with either of two pathogen proteins. This work provides structural insight into how a single host resistance protein can recognize two divergent bacterial effector proteins and supports a proposed mechanism in which each of these effector proteins relieves negative regulation by Pto.
Matsui, Minami
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
Millar, A. Harvey
- You have accessRestricted AccessExploring the Function-Location Nexus: Using Multiple Lines of Evidence in Defining the Subcellular Location of Plant ProteinsA. Harvey Millar, Chris Carrie, Barry Pogson, James WhelanPlant Cell Jun 2009, 21 (6) 1625-1631; DOI: https://doi.org/10.1105/tpc.109.066019
Miyagishima, Shin-ya
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
Moore, Ian
- You have accessRestricted AccessValidating the Location of Fluorescent Protein Fusions in the Endomembrane SystemIan Moore, Angus MurphyPlant Cell Jun 2009, 21 (6) 1632-1636; DOI: https://doi.org/10.1105/tpc.109.068668
Mori, Toshiyuki
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
Moritz, Thomas
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Murphy, Angus
- You have accessRestricted AccessValidating the Location of Fluorescent Protein Fusions in the Endomembrane SystemIan Moore, Angus MurphyPlant Cell Jun 2009, 21 (6) 1632-1636; DOI: https://doi.org/10.1105/tpc.109.068668
Murphy, Angus S.
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
N
Nakai, Masato
- You have accessRestricted AccessA 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope MembraneShingo Kikuchi, Maya Oishi, Yoshino Hirabayashi, Dong Wook Lee, Inhwan Hwang, Masato NakaiPlant Cell Jun 2009, 21 (6) 1781-1797; DOI: https://doi.org/10.1105/tpc.108.063552
This work describes the identification of a 1-MD translocation complex as a novel intermediate during general protein translocation across the inner membrane of chloroplasts. Tic20 and Tic21 are involved in the 1-MD complex, whereas Tic110, the most characterized component of the protein translocon at the inner membrane, exists as a distinct entity from the 1-MD complex.
Nakanishi, Hiromitsu
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
Niyogi, Krishna K.
- Open AccessLutein Accumulation in the Absence of Zeaxanthin Restores Nonphotochemical Quenching in the Arabidopsis thaliana npq1 MutantZhirong Li, Tae Kyu Ahn, Thomas J. Avenson, Matteo Ballottari, Jeffrey A. Cruz, David M. Kramer, Roberto Bassi, Graham R. Fleming, Jay D. Keasling, Krishna K. NiyogiPlant Cell Jun 2009, 21 (6) 1798-1812; DOI: https://doi.org/10.1105/tpc.109.066571
A mutation in the lycopene β-cyclase gene of Arabidopsis increases the accumulation of lutein, which can partially substitute for the role of zeaxanthin in regulation of photosynthetic light harvesting via thermal dissipation of excess absorbed light energy.
O
Oishi, Maya
- You have accessRestricted AccessA 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope MembraneShingo Kikuchi, Maya Oishi, Yoshino Hirabayashi, Dong Wook Lee, Inhwan Hwang, Masato NakaiPlant Cell Jun 2009, 21 (6) 1781-1797; DOI: https://doi.org/10.1105/tpc.108.063552
This work describes the identification of a 1-MD translocation complex as a novel intermediate during general protein translocation across the inner membrane of chloroplasts. Tic20 and Tic21 are involved in the 1-MD complex, whereas Tic110, the most characterized component of the protein translocon at the inner membrane, exists as a distinct entity from the 1-MD complex.
Okazaki, Kumiko
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
Olinares, Paul Dominic B.
- You have accessRestricted AccessSubunits of the Plastid ClpPR Protease Complex Have Differential Contributions to Embryogenesis, Plastid Biogenesis, and Plant Development in ArabidopsisJitae Kim, Andrea Rudella, Verenice Ramirez Rodriguez, Boris Zybailov, Paul Dominic B. Olinares, Klaas J. van WijkPlant Cell Jun 2009, 21 (6) 1669-1692; DOI: https://doi.org/10.1105/tpc.108.063784
Mutant, microscopy, and large-scale comparative proteome analyses of Arabidopsis demonstrate a central role of the Clp protease complex in chloroplast biogenesis and protein homeostasis, with different contributions of the ClpP and ClpR subunits. Lack of transcriptional and translational regulation suggests that regulation of Clp activity occurs through Clp complex assembly and substrate delivery.
Oñate-Sánchez, Luis
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
Otegui, Marisa S.
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
P
Peer, Wendy Ann
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Penfield, Steven
- You have accessRestricted AccessA Role for Multiple Circadian Clock Genes in the Response to Signals That Break Seed Dormancy in ArabidopsisSteven Penfield, Anthony HallPlant Cell Jun 2009, 21 (6) 1722-1732; DOI: https://doi.org/10.1105/tpc.108.064022
After-ripening is a little-known process occurring in dry seeds that results in loss of dormancy upon imbibition. This work shows that after-ripening affects the expression of genes comprising the circadian oscillator and that well-known circadian clock genes have roles in the control of Arabidopsis seed germination.
Petersson, Sara V.
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Pogson, Barry
- You have accessRestricted AccessExploring the Function-Location Nexus: Using Multiple Lines of Evidence in Defining the Subcellular Location of Plant ProteinsA. Harvey Millar, Chris Carrie, Barry Pogson, James WhelanPlant Cell Jun 2009, 21 (6) 1625-1631; DOI: https://doi.org/10.1105/tpc.109.066019
Prade, Elke
- Open AccessThe Multifunctional Enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) Converts Cysteine-Indole-3-Acetonitrile to Camalexin in the Indole-3-Acetonitrile Metabolic Network of Arabidopsis thalianaChristoph Böttcher, Lore Westphal, Constanze Schmotz, Elke Prade, Dierk Scheel, Erich GlawischnigPlant Cell Jun 2009, 21 (6) 1830-1845; DOI: https://doi.org/10.1105/tpc.109.066670
In a comprehensive metabolomics and precursor feeding approach, a network of indole-3-acetonitrile (IAN)–derived metabolites was identified in mutants of camalexin biosynthesis. A Cys conjugate of IAN was shown to be turned over directly to camalexin and cyanide by PHYTOALEXIN DEFICIENT3, a multifunctional cytochrome P450 enzyme.
R
Ramirez Rodriguez, Verenice
- You have accessRestricted AccessSubunits of the Plastid ClpPR Protease Complex Have Differential Contributions to Embryogenesis, Plastid Biogenesis, and Plant Development in ArabidopsisJitae Kim, Andrea Rudella, Verenice Ramirez Rodriguez, Boris Zybailov, Paul Dominic B. Olinares, Klaas J. van WijkPlant Cell Jun 2009, 21 (6) 1669-1692; DOI: https://doi.org/10.1105/tpc.108.063784
Mutant, microscopy, and large-scale comparative proteome analyses of Arabidopsis demonstrate a central role of the Clp protease complex in chloroplast biogenesis and protein homeostasis, with different contributions of the ClpP and ClpR subunits. Lack of transcriptional and translational regulation suggests that regulation of Clp activity occurs through Clp complex assembly and substrate delivery.
Robaglia, Christophe
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
Rollwitz, Inga
- You have accessRestricted AccessThe Plastidic Bile Acid Transporter 5 Is Required for the Biosynthesis of Methionine-Derived Glucosinolates in Arabidopsis thalianaTamara Gigolashvili, Ruslan Yatusevich, Inga Rollwitz, Melanie Humphry, Jonathan Gershenzon, Ulf-Ingo FlüggePlant Cell Jun 2009, 21 (6) 1813-1829; DOI: https://doi.org/10.1105/tpc.109.066399
This work demonstrates that the chloroplastidic BAT5 transporter is required for side chain elongation of Met-derived glucosinolates in Arabidopsis. Comparative feeding experiments of bat5 and wild-type plants using 2-keto acids and amino acids of different chain length suggested that BAT5 functions as a plastidic transporter for 2-keto acids.
Rudella, Andrea
- You have accessRestricted AccessSubunits of the Plastid ClpPR Protease Complex Have Differential Contributions to Embryogenesis, Plastid Biogenesis, and Plant Development in ArabidopsisJitae Kim, Andrea Rudella, Verenice Ramirez Rodriguez, Boris Zybailov, Paul Dominic B. Olinares, Klaas J. van WijkPlant Cell Jun 2009, 21 (6) 1669-1692; DOI: https://doi.org/10.1105/tpc.108.063784
Mutant, microscopy, and large-scale comparative proteome analyses of Arabidopsis demonstrate a central role of the Clp protease complex in chloroplast biogenesis and protein homeostasis, with different contributions of the ClpP and ClpR subunits. Lack of transcriptional and translational regulation suggests that regulation of Clp activity occurs through Clp complex assembly and substrate delivery.
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Sandberg, Göran
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Sankaranarayanan, Rajan
- You have accessRestricted AccessA Cell Wall–Degrading Esterase of Xanthomonas oryzae Requires a Unique Substrate Recognition Module for Pathogenesis on RiceGudlur Aparna, Avradip Chatterjee, Ramesh V. Sonti, Rajan SankaranarayananPlant Cell Jun 2009, 21 (6) 1860-1873; DOI: https://doi.org/10.1105/tpc.109.066886
The structural study of a rice cell wall–degrading esterase LipA from Xanthomonas oryzae pv oryzae, the causal agent of bacterial leaf blight disease, reveals a unique domain that is essential for LipA function in planta and is conserved across the phytopathogenic genus Xanthomonas.
Scheel, Dierk
- Open AccessThe Multifunctional Enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) Converts Cysteine-Indole-3-Acetonitrile to Camalexin in the Indole-3-Acetonitrile Metabolic Network of Arabidopsis thalianaChristoph Böttcher, Lore Westphal, Constanze Schmotz, Elke Prade, Dierk Scheel, Erich GlawischnigPlant Cell Jun 2009, 21 (6) 1830-1845; DOI: https://doi.org/10.1105/tpc.109.066670
In a comprehensive metabolomics and precursor feeding approach, a network of indole-3-acetonitrile (IAN)–derived metabolites was identified in mutants of camalexin biosynthesis. A Cys conjugate of IAN was shown to be turned over directly to camalexin and cyanide by PHYTOALEXIN DEFICIENT3, a multifunctional cytochrome P450 enzyme.
Schmotz, Constanze
- Open AccessThe Multifunctional Enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) Converts Cysteine-Indole-3-Acetonitrile to Camalexin in the Indole-3-Acetonitrile Metabolic Network of Arabidopsis thalianaChristoph Böttcher, Lore Westphal, Constanze Schmotz, Elke Prade, Dierk Scheel, Erich GlawischnigPlant Cell Jun 2009, 21 (6) 1830-1845; DOI: https://doi.org/10.1105/tpc.109.066670
In a comprehensive metabolomics and precursor feeding approach, a network of indole-3-acetonitrile (IAN)–derived metabolites was identified in mutants of camalexin biosynthesis. A Cys conjugate of IAN was shown to be turned over directly to camalexin and cyanide by PHYTOALEXIN DEFICIENT3, a multifunctional cytochrome P450 enzyme.
Sonti, Ramesh V.
- You have accessRestricted AccessA Cell Wall–Degrading Esterase of Xanthomonas oryzae Requires a Unique Substrate Recognition Module for Pathogenesis on RiceGudlur Aparna, Avradip Chatterjee, Ramesh V. Sonti, Rajan SankaranarayananPlant Cell Jun 2009, 21 (6) 1860-1873; DOI: https://doi.org/10.1105/tpc.109.066886
The structural study of a rice cell wall–degrading esterase LipA from Xanthomonas oryzae pv oryzae, the causal agent of bacterial leaf blight disease, reveals a unique domain that is essential for LipA function in planta and is conserved across the phytopathogenic genus Xanthomonas.
Sormani, Rodnay
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
Suzuki, Kenji
- You have accessRestricted AccessThe PLASTID DIVISION1 and 2 Components of the Chloroplast Division Machinery Determine the Rate of Chloroplast Division in Land Plant Cell DifferentiationKumiko Okazaki, Yukihiro Kabeya, Kenji Suzuki, Toshiyuki Mori, Takanari Ichikawa, Minami Matsui, Hiromitsu Nakanishi, Shin-ya MiyagishimaPlant Cell Jun 2009, 21 (6) 1769-1780; DOI: https://doi.org/10.1105/tpc.109.067785
In contrast with most algae, in which chloroplast division keeps pace with cell division, land plant cells change the chloroplast division rate (i.e., size and number of chloroplasts) in accord with cell differentiation. This study suggests that acquisition of PLASTID DIVISION proteins by land plants enabled cells to change the chloroplast division rate based on cell differentiation.
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Titapiwatanakun, Boosaree
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
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van Wijk, Klaas J.
- You have accessRestricted AccessSubunits of the Plastid ClpPR Protease Complex Have Differential Contributions to Embryogenesis, Plastid Biogenesis, and Plant Development in ArabidopsisJitae Kim, Andrea Rudella, Verenice Ramirez Rodriguez, Boris Zybailov, Paul Dominic B. Olinares, Klaas J. van WijkPlant Cell Jun 2009, 21 (6) 1669-1692; DOI: https://doi.org/10.1105/tpc.108.063784
Mutant, microscopy, and large-scale comparative proteome analyses of Arabidopsis demonstrate a central role of the Clp protease complex in chloroplast biogenesis and protein homeostasis, with different contributions of the ClpP and ClpR subunits. Lack of transcriptional and translational regulation suggests that regulation of Clp activity occurs through Clp complex assembly and substrate delivery.
Vicente-Carbajosa, Jesús
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
Voinnet, Olivier
- You have accessRestricted AccessBiochemical Evidence for Translational Repression by Arabidopsis MicroRNAsElodie Lanet, Etienne Delannoy, Rodnay Sormani, Maïna Floris, Peter Brodersen, Patrice Crété, Olivier Voinnet, Christophe RobagliaPlant Cell Jun 2009, 21 (6) 1762-1768; DOI: https://doi.org/10.1105/tpc.108.063412
This work provides biochemical evidence of the association of miRNAs with translating polysomes in Arabidopsis. These data support the idea that RNA silencing mechanisms in animal and plant kingdoms are more similar than previously thought.
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Wang, Jean Y.
- You have accessRestricted AccessAn Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and SynthesisSara V. Petersson, Annika I. Johansson, Mariusz Kowalczyk, Alexander Makoveychuk, Jean Y. Wang, Thomas Moritz, Markus Grebe, Philip N. Benfey, Göran Sandberg, Karin LjungPlant Cell Jun 2009, 21 (6) 1659-1668; DOI: https://doi.org/10.1105/tpc.109.066480
This study describes a novel method for cell-specific analysis of the important plant hormone auxin. Cell sorting on cell type–specific tagged lines followed by precise mass spectrometry measurements were used to produce a high-resolution auxin distribution map of the Arabidopsis root, demonstrating the existence of an auxin gradient and maximum and sites of high auxin biosynthesis rates.
Weltmeier, Fridtjof
- You have accessRestricted AccessA Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex FormationRosario Alonso, Luis Oñate-Sánchez, Fridtjof Weltmeier, Andrea Ehlert, Isabel Diaz, Katrin Dietrich, Jesús Vicente-Carbajosa, Wolfgang Dröge-LaserPlant Cell Jun 2009, 21 (6) 1747-1761; DOI: https://doi.org/10.1105/tpc.108.062968
This work identifies the Arabidopsis basic leucine zipper protein bZIP53 as a regulator of seed maturation genes. The mechanism of transcriptional control is defined by heterodimerization with bZIP10 or bZIP25 and ternary complex formation with ABI3.
Westphal, Lore
- Open AccessThe Multifunctional Enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) Converts Cysteine-Indole-3-Acetonitrile to Camalexin in the Indole-3-Acetonitrile Metabolic Network of Arabidopsis thalianaChristoph Böttcher, Lore Westphal, Constanze Schmotz, Elke Prade, Dierk Scheel, Erich GlawischnigPlant Cell Jun 2009, 21 (6) 1830-1845; DOI: https://doi.org/10.1105/tpc.109.066670
In a comprehensive metabolomics and precursor feeding approach, a network of indole-3-acetonitrile (IAN)–derived metabolites was identified in mutants of camalexin biosynthesis. A Cys conjugate of IAN was shown to be turned over directly to camalexin and cyanide by PHYTOALEXIN DEFICIENT3, a multifunctional cytochrome P450 enzyme.
Whelan, James
- You have accessRestricted AccessExploring the Function-Location Nexus: Using Multiple Lines of Evidence in Defining the Subcellular Location of Plant ProteinsA. Harvey Millar, Chris Carrie, Barry Pogson, James WhelanPlant Cell Jun 2009, 21 (6) 1625-1631; DOI: https://doi.org/10.1105/tpc.109.066019
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Xiao, Fangming
- You have accessRestricted AccessCrystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-PtoJing Dong, Fangming Xiao, Fenxia Fan, Lichuan Gu, Huaixing Cang, Gregory B. Martin, Jijie ChaiPlant Cell Jun 2009, 21 (6) 1846-1859; DOI: https://doi.org/10.1105/tpc.109.066878
Resistance to bacterial speck disease in tomato is activated upon interaction of the host Pto kinase with either of two pathogen proteins. This work provides structural insight into how a single host resistance protein can recognize two divergent bacterial effector proteins and supports a proposed mechanism in which each of these effector proteins relieves negative regulation by Pto.
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Yakubov, Bahktiyor
- You have accessRestricted AccessMutation of the Membrane-Associated M1 Protease APM1 Results in Distinct Embryonic and Seedling Developmental Defects in ArabidopsisWendy Ann Peer, Fazeeda N. Hosein, Anindita Bandyopadhyay, Srinivas N. Makam, Marisa S. Otegui, Gil-Je Lee, Joshua J. Blakeslee, Yan Cheng, Boosaree Titapiwatanakun, Bahktiyor Yakubov, Bharat Bangari, Angus S. MurphyPlant Cell Jun 2009, 21 (6) 1693-1721; DOI: https://doi.org/10.1105/tpc.108.059634
Analysis of apm1 loss-of-function mutants and inducible expression/silencing transformants indicate that this M1 metallopeptidase is required for root meristem initiation during embryogenesis and meristem maintenance during seedling establishment. Meristem identity and maintenance also require auxin, and APM1 is a low-affinity target of the auxin transport inhibitor NPA.
Yatusevich, Ruslan
- You have accessRestricted AccessThe Plastidic Bile Acid Transporter 5 Is Required for the Biosynthesis of Methionine-Derived Glucosinolates in Arabidopsis thalianaTamara Gigolashvili, Ruslan Yatusevich, Inga Rollwitz, Melanie Humphry, Jonathan Gershenzon, Ulf-Ingo FlüggePlant Cell Jun 2009, 21 (6) 1813-1829; DOI: https://doi.org/10.1105/tpc.109.066399
This work demonstrates that the chloroplastidic BAT5 transporter is required for side chain elongation of Met-derived glucosinolates in Arabidopsis. Comparative feeding experiments of bat5 and wild-type plants using 2-keto acids and amino acids of different chain length suggested that BAT5 functions as a plastidic transporter for 2-keto acids.
Z
Zybailov, Boris
- You have accessRestricted AccessSubunits of the Plastid ClpPR Protease Complex Have Differential Contributions to Embryogenesis, Plastid Biogenesis, and Plant Development in ArabidopsisJitae Kim, Andrea Rudella, Verenice Ramirez Rodriguez, Boris Zybailov, Paul Dominic B. Olinares, Klaas J. van WijkPlant Cell Jun 2009, 21 (6) 1669-1692; DOI: https://doi.org/10.1105/tpc.108.063784
Mutant, microscopy, and large-scale comparative proteome analyses of Arabidopsis demonstrate a central role of the Clp protease complex in chloroplast biogenesis and protein homeostasis, with different contributions of the ClpP and ClpR subunits. Lack of transcriptional and translational regulation suggests that regulation of Clp activity occurs through Clp complex assembly and substrate delivery.