Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
OtherResearch Article
You have accessRestricted Access

Genome-Wide Medicago truncatula Small RNA Analysis Revealed Novel MicroRNAs and Isoforms Differentially Regulated in Roots and Nodules

Christine Lelandais-Brière, Loreto Naya, Erika Sallet, Fanny Calenge, Florian Frugier, Caroline Hartmann, Jérome Gouzy, Martin Crespi
Christine Lelandais-Brière
aInstitut des Sciences du Végétal, Centre National de la Recherche Scientifique, F-91198 Gif-sur-Yvette Cedex, France
bUniversité Paris Diderot-Paris 7, 75205 Paris Cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Loreto Naya
aInstitut des Sciences du Végétal, Centre National de la Recherche Scientifique, F-91198 Gif-sur-Yvette Cedex, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Erika Sallet
cLaboratoire des Interactions Plantes Micro-organismes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique–Institut National de la Recherche Agronomique 2594/441, F- 31320 Castanet Tolosan, France
dPlateforme Bioinformatique du Génopole Toulouse Midi-Pyrénées, Groupement d'Intérêt Scientifique Toulouse Genopole, F-31320 Castanet Tolosan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fanny Calenge
aInstitut des Sciences du Végétal, Centre National de la Recherche Scientifique, F-91198 Gif-sur-Yvette Cedex, France
bUniversité Paris Diderot-Paris 7, 75205 Paris Cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Florian Frugier
aInstitut des Sciences du Végétal, Centre National de la Recherche Scientifique, F-91198 Gif-sur-Yvette Cedex, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caroline Hartmann
aInstitut des Sciences du Végétal, Centre National de la Recherche Scientifique, F-91198 Gif-sur-Yvette Cedex, France
bUniversité Paris Diderot-Paris 7, 75205 Paris Cedex 13, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jérome Gouzy
cLaboratoire des Interactions Plantes Micro-organismes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique–Institut National de la Recherche Agronomique 2594/441, F- 31320 Castanet Tolosan, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Martin Crespi
aInstitut des Sciences du Végétal, Centre National de la Recherche Scientifique, F-91198 Gif-sur-Yvette Cedex, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published September 2009. DOI: https://doi.org/10.1105/tpc.109.068130

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • American Society of Plant Biologists

Abstract

Posttranscriptional regulation of a variety of mRNAs by small 21- to 24-nucleotide RNAs, notably the microRNAs (miRNAs), is emerging as a novel developmental mechanism. In legumes like the model Medicago truncatula, roots are able to develop a de novo meristem through the symbiotic interaction with nitrogen-fixing rhizobia. We used deep sequencing of small RNAs from root apexes and nodules of M. truncatula to identify 100 novel candidate miRNAs encoded by 265 hairpin precursors. New atypical precursor classes producing only specific 21- and 24-nucleotide small RNAs were found. Statistical analysis on sequencing reads abundance revealed specific miRNA isoforms in a same family showing contrasting expression patterns between nodules and root apexes. The differentially expressed conserved and nonconserved miRNAs may target a large variety of mRNAs. In root nodules, which show diverse cell types ranging from a persistent meristem to a fully differentiated central region, we discovered miRNAs spatially enriched in nodule meristematic tissues, vascular bundles, and bacterial infection zones using in situ hybridization. Spatial regulation of miRNAs may determine specialization of regulatory RNA networks in plant differentiation processes, such as root nodule formation.

  • Received April 23, 2009.
  • Revised July 9, 2009.
  • Accepted July 26, 2009.
  • Published September 18, 2009.
View Full Text
PreviousNext
Back to top

Table of Contents

Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Cell.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Genome-Wide Medicago truncatula Small RNA Analysis Revealed Novel MicroRNAs and Isoforms Differentially Regulated in Roots and Nodules
(Your Name) has sent you a message from Plant Cell
(Your Name) thought you would like to see the Plant Cell web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Genome-Wide Medicago truncatula Small RNA Analysis Revealed Novel MicroRNAs and Isoforms Differentially Regulated in Roots and Nodules
Christine Lelandais-Brière, Loreto Naya, Erika Sallet, Fanny Calenge, Florian Frugier, Caroline Hartmann, Jérome Gouzy, Martin Crespi
The Plant Cell Sep 2009, 21 (9) 2780-2796; DOI: 10.1105/tpc.109.068130

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Genome-Wide Medicago truncatula Small RNA Analysis Revealed Novel MicroRNAs and Isoforms Differentially Regulated in Roots and Nodules
Christine Lelandais-Brière, Loreto Naya, Erika Sallet, Fanny Calenge, Florian Frugier, Caroline Hartmann, Jérome Gouzy, Martin Crespi
The Plant Cell Sep 2009, 21 (9) 2780-2796; DOI: 10.1105/tpc.109.068130
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • METHODS
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

The Plant Cell Online: 21 (9)
The Plant Cell
Vol. 21, Issue 9
September 2009
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Advertising (PDF)
  • Front Matter (PDF)
View this article with LENS

More in this TOC Section

  • Abscisic Acid-Triggered Persulfidation of the Cys Protease ATG4 Mediates Regulation of Autophagy by Sulfide
  • Temporal Regulation of the Metabolome and Proteome in Photosynthetic and Photorespiratory Pathways Contributes to Maize Heterosis
  • Chloroplast Chaperonin-Mediated Targeting of a Thylakoid Membrane Protein
Show more RESEARCH ARTICLES

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Cell Preview
  • Archive
  • Teaching Tools in Plant Biology
  • Plant Physiology
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Peer Review Reports
  • Journal Miles
  • Transfer of reviews to Plant Direct
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds
  • Contact Us

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire