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Actin Interacting Protein1 and Actin Depolymerizing Factor Drive Rapid Actin Dynamics in Physcomitrella patens

Robert C. Augustine, Kelli A. Pattavina, Erkan Tüzel, Luis Vidali, Magdalena Bezanilla
Robert C. Augustine
aBiology Department, University of Massachusetts, Amherst, Massachusetts 01003
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Kelli A. Pattavina
aBiology Department, University of Massachusetts, Amherst, Massachusetts 01003
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Erkan Tüzel
bDepartment of Physics, Worcester Polytechnic Institute, Worcester, Massachusetts 01609
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Luis Vidali
cDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609
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Magdalena Bezanilla
aBiology Department, University of Massachusetts, Amherst, Massachusetts 01003
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  • For correspondence: bezanilla@bio.umass.edu

Published October 2011. DOI: https://doi.org/10.1105/tpc.111.090753

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  • © 2011 American Society of Plant Biologists. All rights reserved.

Abstract

The remodeling of actin networks is required for a variety of cellular processes in eukaryotes. In plants, several actin binding proteins have been implicated in remodeling cortical actin filaments (F-actin). However, the extent to which these proteins support F-actin dynamics in planta has not been tested. Using reverse genetics, complementation analyses, and cell biological approaches, we assessed the in vivo function of two actin turnover proteins: actin interacting protein1 (AIP1) and actin depolymerizing factor (ADF). We report that AIP1 is a single-copy gene in the moss Physcomitrella patens. AIP1 knockout plants are viable but have reduced expansion of tip-growing cells. AIP1 is diffusely cytosolic and functions in a common genetic pathway with ADF to promote tip growth. Specifically, ADF can partially compensate for loss of AIP1, and AIP1 requires ADF for function. Consistent with a role in actin remodeling, AIP1 knockout lines accumulate F-actin bundles, have fewer dynamic ends, and have reduced severing frequency. Importantly, we demonstrate that AIP1 promotes and ADF is essential for cortical F-actin dynamics.

  • Received August 19, 2011.
  • Revised September 20, 2011.
  • Accepted October 2, 2011.
  • Published October 14, 2011.
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Actin Interacting Protein1 and Actin Depolymerizing Factor Drive Rapid Actin Dynamics in Physcomitrella patens
Robert C. Augustine, Kelli A. Pattavina, Erkan Tüzel, Luis Vidali, Magdalena Bezanilla
The Plant Cell Oct 2011, 23 (10) 3696-3710; DOI: 10.1105/tpc.111.090753

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Actin Interacting Protein1 and Actin Depolymerizing Factor Drive Rapid Actin Dynamics in Physcomitrella patens
Robert C. Augustine, Kelli A. Pattavina, Erkan Tüzel, Luis Vidali, Magdalena Bezanilla
The Plant Cell Oct 2011, 23 (10) 3696-3710; DOI: 10.1105/tpc.111.090753
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