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RopGEF7 Regulates PLETHORA-Dependent Maintenance of the Root Stem Cell Niche in Arabidopsis

Min Chen, Huili Liu, Jixiang Kong, Yali Yang, Naichao Zhang, Ruijing Li, Jianbin Yue, Jiaqing Huang, Chuanyou Li, Alice Y. Cheung, Li-zhen Tao
Min Chen
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Huili Liu
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Jixiang Kong
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Yali Yang
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Naichao Zhang
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Ruijing Li
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Jianbin Yue
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Jiaqing Huang
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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Chuanyou Li
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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Alice Y. Cheung
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003
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Li-zhen Tao
Key Laboratory of Ministry of Education for Rice Fertility Development and Stress Resistance, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
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  • For correspondence: lztao2005@yahoo.com

Published August 2011. DOI: https://doi.org/10.1105/tpc.111.085514

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  • © 2011 American Society of Plant Biologists. All rights reserved.

Abstract

The root stem cell niche defines the area that specifies and maintains the stem cells and is essential for the maintenance of root growth. Here, we characterize and examine the functional role of a quiescent center (QC)–expressed RAC/ROP GTPase activator, RopGEF7, in Arabidopsis thaliana. We show that RopGEF7 interacts with At RAC1 and overexpression of a C-terminally truncated constitutively active RopGEF7 (RopGEF7ΔC) activates RAC/ROP GTPases. Knockdown of RopGEF7 by RNA interference causes defects in embryo patterning and maintenance of the QC and leads to postembryonic loss of root stem cell population. Gene expression studies indicate that RopGEF7 is required for root meristem maintenance as it regulates the expression of PLETHORA1 (PLT1) and PLT2, which are key transcription factors that mediate the patterning of the root stem cell niche. Genetic analyses show that RopGEF7 interacts with PLT genes to regulate QC maintenance. Moreover, RopGEF7 is induced transcriptionally by auxin while its function is required for the expression of the auxin efflux protein PIN1 and maintenance of normal auxin maxima in embryos and seedling roots. These results suggest that RopGEF7 may integrate auxin-derived positional information in a feed-forward mechanism, regulating PLT transcription factors and thereby controlling the maintenance of root stem cell niches.

  • Received March 24, 2011.
  • Revised June 16, 2011.
  • Accepted July 17, 2011.
  • Published August 9, 2011.

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RopGEF7 Regulates PLETHORA-Dependent Maintenance of the Root Stem Cell Niche in Arabidopsis
Min Chen, Huili Liu, Jixiang Kong, Yali Yang, Naichao Zhang, Ruijing Li, Jianbin Yue, Jiaqing Huang, Chuanyou Li, Alice Y. Cheung, Li-zhen Tao
The Plant Cell Aug 2011, 23 (8) 2880-2894; DOI: 10.1105/tpc.111.085514

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RopGEF7 Regulates PLETHORA-Dependent Maintenance of the Root Stem Cell Niche in Arabidopsis
Min Chen, Huili Liu, Jixiang Kong, Yali Yang, Naichao Zhang, Ruijing Li, Jianbin Yue, Jiaqing Huang, Chuanyou Li, Alice Y. Cheung, Li-zhen Tao
The Plant Cell Aug 2011, 23 (8) 2880-2894; DOI: 10.1105/tpc.111.085514
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The Plant Cell Online: 23 (8)
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Vol. 23, Issue 8
Aug 2011
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