Table of Contents
A
Abu el Heba, Ghada
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Afzal, Ahmed J.
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Álvarez, Consolación
- You have accessRestricted AccessCysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in ArabidopsisConsolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia GotorPlant Cell Nov 2012, 24 (11) 4621-4634; DOI: https://doi.org/10.1105/tpc.112.105403
This article highlights the role of hydrogen sulfide as a relevant signaling molecule in plants, of comparable importance as described in animals. This study shows the regulatory role of sulfide generated by the cytosolic l-Cys desulfhydrase 1 enzyme on autophagy in eukaryotes.
Amar, David
- You have accessRestricted AccessThe MORPH Algorithm: Ranking Candidate Genes for Membership in Arabidopsis and Tomato PathwaysOren Tzfadia, David Amar, Louis M.T. Bradbury, Eleanore T. Wurtzel, Ron ShamirPlant Cell Nov 2012, 24 (11) 4389-4406; DOI: https://doi.org/10.1105/tpc.112.104513
A fundamental challenge in plant biology is to discover the unknown connections within and between pathways and to associate previously unknown genes with specific biological processes. MORPH is a computational method for predicting genes that function in or regulate a biological pathway. MORPH fills pathway gaps and defines complex biological networks as shown for Arabidopsis and tomato.
Ambrose, Mike
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Anderson, Ryan G.
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Angenent, Gerco C.
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Arenas-Huertero, Catalina
- You have accessRestricted AccessGenome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in ArabidopsisJun Liu, Choonkyun Jung, Jun Xu, Huan Wang, Shulin Deng, Lucia Bernad, Catalina Arenas-Huertero, Nam-Hai ChuaPlant Cell Nov 2012, 24 (11) 4333-4345; DOI: https://doi.org/10.1105/tpc.112.102855
This work identifies 6480 long intergenic noncoding RNAs in Arabidopsis, many of which show organ-specific and stress-responsive expression. The biogenesis of a group of long intergenic noncoding RNAs is coregulated by the RNA processing proteins SERRATE, CBP20, and CBP80.
B
Ballester, Ana Rosa
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Bassard, Jean-Etienne
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Bassham, Diane C.
- You have accessRestricted AccessDegradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in ArabidopsisYimo Liu, Junmarie Soto Burgos, Yan Deng, Renu Srivastava, Stephen H. Howell, Diane C. BasshamPlant Cell Nov 2012, 24 (11) 4635-4651; DOI: https://doi.org/10.1105/tpc.112.101535
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), cells activate an ER stress response to enable plants to tolerate these conditions. This work shows that one facet of this response is the activation of the autophagy pathway for degradation of ER fragments in the vacuole, which is regulated by the IRE1b splicing factor.
Bellizzi, Maria
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Bemer, Marian
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Benning, Christoph
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
Berendzen, Kenneth Wayne
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Bernad, Lucia
- You have accessRestricted AccessGenome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in ArabidopsisJun Liu, Choonkyun Jung, Jun Xu, Huan Wang, Shulin Deng, Lucia Bernad, Catalina Arenas-Huertero, Nam-Hai ChuaPlant Cell Nov 2012, 24 (11) 4333-4345; DOI: https://doi.org/10.1105/tpc.112.102855
This work identifies 6480 long intergenic noncoding RNAs in Arabidopsis, many of which show organ-specific and stress-responsive expression. The biogenesis of a group of long intergenic noncoding RNAs is coregulated by the RNA processing proteins SERRATE, CBP20, and CBP80.
Berson, Tobias
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Boerjan, Wout
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Borisov, Alexei Y.
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Böttcher, Christine
- You have accessRestricted AccessCrystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin HomeostasisThomas S. Peat, Christine Böttcher, Janet Newman, Del Lucent, Nathan Cowieson, Christopher DaviesPlant Cell Nov 2012, 24 (11) 4525-4538; DOI: https://doi.org/10.1105/tpc.112.102921
The crystal structure of an indole-3-acetic acid amido synthetase from Vitis vinifera involved in auxin homeostasis is presented. Residues likely to be involved in acyl group, amino acid, and ATP substrate binding have been identified, and this information provides a tool for designing new, effective auxins.
Bovy, Arnaud G.
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Braam, Janet
- You have accessRestricted AccessXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in ArabidopsisXiao Fang Zhu, Yuan Zhi Shi, Gui Jie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu, Shao Jian ZhengPlant Cell Nov 2012, 24 (11) 4731-4747; DOI: https://doi.org/10.1105/tpc.112.106039
Hemicellulose can retain a large amount of Al. This study demonstrates that an important component of hemicellulose, xyloglucan, can bind Al, and knockout of XTH31 increases Al resistance in Arabidopsis by decreasing xyloglucan endotransglucosylase action and the accumulation of xyloglucan in hemicellulose, which in turn reduces the retention of Al in the cell wall, thus excluding Al from roots.
Bradbury, Louis M.T.
- You have accessRestricted AccessThe MORPH Algorithm: Ranking Candidate Genes for Membership in Arabidopsis and Tomato PathwaysOren Tzfadia, David Amar, Louis M.T. Bradbury, Eleanore T. Wurtzel, Ron ShamirPlant Cell Nov 2012, 24 (11) 4389-4406; DOI: https://doi.org/10.1105/tpc.112.104513
A fundamental challenge in plant biology is to discover the unknown connections within and between pathways and to associate previously unknown genes with specific biological processes. MORPH is a computational method for predicting genes that function in or regulate a biological pathway. MORPH fills pathway gaps and defines complex biological networks as shown for Arabidopsis and tomato.
Briggs, Steven P.
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
Burgos, Junmarie Soto
- You have accessRestricted AccessDegradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in ArabidopsisYimo Liu, Junmarie Soto Burgos, Yan Deng, Renu Srivastava, Stephen H. Howell, Diane C. BasshamPlant Cell Nov 2012, 24 (11) 4635-4651; DOI: https://doi.org/10.1105/tpc.112.101535
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), cells activate an ER stress response to enable plants to tolerate these conditions. This work shows that one facet of this response is the activation of the autophagy pathway for degradation of ER fragments in the vacuole, which is regulated by the IRE1b splicing factor.
C
Cai, Chao
- Open AccessFIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin FringeHui Su, Jinsheng Zhu, Chao Cai, Weike Pei, Jiaojiao Wang, Huaijian Dong, Haiyun RenPlant Cell Nov 2012, 24 (11) 4539-4554; DOI: https://doi.org/10.1105/tpc.112.099358
Fimbrin family proteins have long been known as actin binding proteins that regulate the formation of higher-order actin filament structures. FIM1, a fimbrin homolog from Lilium longiflorum, is found to cross-link actin microfilaments into bundles in vitro and is demonstrated to play important roles in the maintenance of the actin fringe in pollen tubes.
Cannon, Steven B.
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Cao, Xiaofeng
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Carlsson, Anders S.
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Chandrasekar, Balakumaran
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Chen, Guanqun
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Chen, Haodong
- Open AccessArabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B LightXi Huang, Xinhao Ouyang, Panyu Yang, On Sun Lau, Gang Li, Jigang Li, Haodong Chen, Xing Wang DengPlant Cell Nov 2012, 24 (11) 4590-4606; DOI: https://doi.org/10.1105/tpc.112.103994
In UV-B–induced photomorphogenesis in Arabidopsis, COP1 is a UV-B–inducible gene and FHY3 and HY5 directly activate COP1, dependent on UV-B, by binding to the COP1 promoter to ensure photomorphogenic UV-B signaling. The working mode of FHY3 and HY5 in UV-B–specific signaling is distinct from that in far-red light and circadian conditions.
Chen, Qilin
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Chen, Songbiao
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Chen, Ying
- Open AccessRegulatory Impact of RNA Secondary Structure across the Arabidopsis TranscriptomeFan Li, Qi Zheng, Lee E. Vandivier, Matthew R. Willmann, Ying Chen, Brian D. GregoryPlant Cell Nov 2012, 24 (11) 4346-4359; DOI: https://doi.org/10.1105/tpc.112.104232
This study employs a high-throughput, sequencing-based, structure-mapping approach to investigate RNA secondary structure throughout the Arabidopsis transcriptome. By combining these structural insights with a number of other RNA sequencing–based approaches, it provides a global assessment of RNA folding and its significant regulatory effects in a plant transcriptome.
Cheng, Jiye
- Open AccessThe Coronatine Toxin of Pseudomonas syringae Is a Multifunctional Suppressor of Arabidopsis DefenseXueqing Geng, Jiye Cheng, Anju Gangadharan, David MackeyPlant Cell Nov 2012, 24 (11) 4763-4774; DOI: https://doi.org/10.1105/tpc.112.105312
The bacterial phytotoxin coronatine mimics a plant hormone, jasmonic acid, and thus antagonizes signaling by another plant hormone, salicylic acid, important for plant defense. This study demonstrates that coronatine also suppresses SA-independent host defense, including the production of defense-promoting indole glucosinolates and has a target other than the jasmonic acid receptor.
Cheng, Zhijun
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Chua, Nam-Hai
- You have accessRestricted AccessGenome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in ArabidopsisJun Liu, Choonkyun Jung, Jun Xu, Huan Wang, Shulin Deng, Lucia Bernad, Catalina Arenas-Huertero, Nam-Hai ChuaPlant Cell Nov 2012, 24 (11) 4333-4345; DOI: https://doi.org/10.1105/tpc.112.102855
This work identifies 6480 long intergenic noncoding RNAs in Arabidopsis, many of which show organ-specific and stress-responsive expression. The biogenesis of a group of long intergenic noncoding RNAs is coregulated by the RNA processing proteins SERRATE, CBP20, and CBP80.
Coerper, Daniel
- You have accessRestricted AccessThe Novel Plant Protein INAPERTURATE POLLEN1 Marks Distinct Cellular Domains and Controls Formation of Apertures in the Arabidopsis Pollen ExineAnna A. Dobritsa, Daniel CoerperPlant Cell Nov 2012, 24 (11) 4452-4464; DOI: https://doi.org/10.1105/tpc.112.101220
Pollen grains are protected by beautiful and elaborate cell walls, exines. This work examines the formation of one distinct patterning element of pollen surfaces, apertures, or areas where exine is not deposited. It demonstrates that the formation of apertures depends on the novel plant protein INP1, which is directed to aperture areas and regulates aperture length in a dosage-dependent manner.
Cosson, Viviane
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Couzigou, Jean-Malo
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Cowieson, Nathan
- You have accessRestricted AccessCrystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin HomeostasisThomas S. Peat, Christine Böttcher, Janet Newman, Del Lucent, Nathan Cowieson, Christopher DaviesPlant Cell Nov 2012, 24 (11) 4525-4538; DOI: https://doi.org/10.1105/tpc.112.102921
The crystal structure of an indole-3-acetic acid amido synthetase from Vitis vinifera involved in auxin homeostasis is presented. Residues likely to be involved in acyl group, amino acid, and ATP substrate binding have been identified, and this information provides a tool for designing new, effective auxins.
Crespo, José L.
- You have accessRestricted AccessCysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in ArabidopsisConsolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia GotorPlant Cell Nov 2012, 24 (11) 4621-4634; DOI: https://doi.org/10.1105/tpc.112.105403
This article highlights the role of hydrogen sulfide as a relevant signaling molecule in plants, of comparable importance as described in animals. This study shows the regulatory role of sulfide generated by the cytosolic l-Cys desulfhydrase 1 enzyme on autophagy in eukaryotes.
Cui, Xiekui
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
D
Davies, Christopher
- You have accessRestricted AccessCrystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin HomeostasisThomas S. Peat, Christine Böttcher, Janet Newman, Del Lucent, Nathan Cowieson, Christopher DaviesPlant Cell Nov 2012, 24 (11) 4525-4538; DOI: https://doi.org/10.1105/tpc.112.102921
The crystal structure of an indole-3-acetic acid amido synthetase from Vitis vinifera involved in auxin homeostasis is presented. Residues likely to be involved in acyl group, amino acid, and ATP substrate binding have been identified, and this information provides a tool for designing new, effective auxins.
De Jaeger, Geert
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
de Maagd, Ruud A.
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Deng, Shulin
- You have accessRestricted AccessGenome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in ArabidopsisJun Liu, Choonkyun Jung, Jun Xu, Huan Wang, Shulin Deng, Lucia Bernad, Catalina Arenas-Huertero, Nam-Hai ChuaPlant Cell Nov 2012, 24 (11) 4333-4345; DOI: https://doi.org/10.1105/tpc.112.102855
This work identifies 6480 long intergenic noncoding RNAs in Arabidopsis, many of which show organ-specific and stress-responsive expression. The biogenesis of a group of long intergenic noncoding RNAs is coregulated by the RNA processing proteins SERRATE, CBP20, and CBP80.
Deng, Xing Wang
- Open AccessArabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B LightXi Huang, Xinhao Ouyang, Panyu Yang, On Sun Lau, Gang Li, Jigang Li, Haodong Chen, Xing Wang DengPlant Cell Nov 2012, 24 (11) 4590-4606; DOI: https://doi.org/10.1105/tpc.112.103994
In UV-B–induced photomorphogenesis in Arabidopsis, COP1 is a UV-B–inducible gene and FHY3 and HY5 directly activate COP1, dependent on UV-B, by binding to the COP1 promoter to ensure photomorphogenic UV-B signaling. The working mode of FHY3 and HY5 in UV-B–specific signaling is distinct from that in far-red light and circadian conditions.
Deng, Yan
- You have accessRestricted AccessDegradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in ArabidopsisYimo Liu, Junmarie Soto Burgos, Yan Deng, Renu Srivastava, Stephen H. Howell, Diane C. BasshamPlant Cell Nov 2012, 24 (11) 4635-4651; DOI: https://doi.org/10.1105/tpc.112.101535
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), cells activate an ER stress response to enable plants to tolerate these conditions. This work shows that one facet of this response is the activation of the autophagy pathway for degradation of ER fragments in the vacuole, which is regulated by the IRE1b splicing factor.
Dobritsa, Anna A.
- You have accessRestricted AccessThe Novel Plant Protein INAPERTURATE POLLEN1 Marks Distinct Cellular Domains and Controls Formation of Apertures in the Arabidopsis Pollen ExineAnna A. Dobritsa, Daniel CoerperPlant Cell Nov 2012, 24 (11) 4452-4464; DOI: https://doi.org/10.1105/tpc.112.101220
Pollen grains are protected by beautiful and elaborate cell walls, exines. This work examines the formation of one distinct patterning element of pollen surfaces, apertures, or areas where exine is not deposited. It demonstrates that the formation of apertures depends on the novel plant protein INP1, which is directed to aperture areas and regulates aperture length in a dosage-dependent manner.
Dong, Huaijian
- Open AccessFIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin FringeHui Su, Jinsheng Zhu, Chao Cai, Weike Pei, Jiaojiao Wang, Huaijian Dong, Haiyun RenPlant Cell Nov 2012, 24 (11) 4539-4554; DOI: https://doi.org/10.1105/tpc.112.099358
Fimbrin family proteins have long been known as actin binding proteins that regulate the formation of higher-order actin filament structures. FIM1, a fimbrin homolog from Lilium longiflorum, is found to cross-link actin microfilaments into bundles in vitro and is demonstrated to play important roles in the maintenance of the actin fringe in pollen tubes.
Drechsel, Gabriele
- You have accessRestricted AccessPolypyrimidine Tract Binding Protein Homologs from Arabidopsis Are Key Regulators of Alternative Splicing with Implications in Fundamental Developmental ProcessesChristina Rühl, Eva Stauffer, André Kahles, Gabriele Wagner, Gabriele Drechsel, Gunnar Rätsch, Andreas WachterPlant Cell Nov 2012, 24 (11) 4360-4375; DOI: https://doi.org/10.1105/tpc.112.103622
Alternative precursor mRNA splicing massively expands the transcriptome diversity in higher eukaryotes. In this report, we describe Arabidopsis Polypyrimidine tract binding protein homologs as critical components of the plant splicing code and provide links between regulated alternative splicing events and seed germination as well as flowering time control.
Du, Jianchang
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Duval, Frédéric
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
E
Ellis, T.H. Noel
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
F
Facette, Michelle
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
Farquharson, Kathleen L.
- You have accessRestricted AccessPolarization of Subsidiary Cell Division in Maize Stomatal ComplexesKathleen L. FarquharsonPlant Cell Nov 2012, 24 (11) 4313; DOI: https://doi.org/10.1105/tpc.112.241112
Fedewa, Marie
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
Frantzeskakis, Lamprinos
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Fry, Stephen C.
- You have accessRestricted AccessXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in ArabidopsisXiao Fang Zhu, Yuan Zhi Shi, Gui Jie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu, Shao Jian ZhengPlant Cell Nov 2012, 24 (11) 4731-4747; DOI: https://doi.org/10.1105/tpc.112.106039
Hemicellulose can retain a large amount of Al. This study demonstrates that an important component of hemicellulose, xyloglucan, can bind Al, and knockout of XTH31 increases Al resistance in Arabidopsis by decreasing xyloglucan endotransglucosylase action and the accumulation of xyloglucan in hemicellulose, which in turn reduces the retention of Al in the cell wall, thus excluding Al from roots.
Fujiwara, Sumire
- Open AccessA Triantagonistic Basic Helix-Loop-Helix System Regulates Cell Elongation in ArabidopsisMiho Ikeda, Sumire Fujiwara, Nobutaka Mitsuda, Masaru Ohme-TakagiPlant Cell Nov 2012, 24 (11) 4483-4497; DOI: https://doi.org/10.1105/tpc.112.105023
This work identifies three basic helix-loop-helix (bHLH) transcription factors that competitively regulate cell elongation. In this tri-antagonistic system, a negative regulatory bHLH interacts with and interferes with the function of an activator bHLH that activates genes for cell elongation. A third bHLH inactivates the negative bHLH; this double negative regulation activates cell elongation.
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Gaffney, Bobby
- Open AccessGenome-Wide Control of Polyadenylation Site Choice by CPSF30 in ArabidopsisPatrick E. Thomas, Xiaohui Wu, Man Liu, Bobby Gaffney, Guoli Ji, Qingshun Q. Li, Arthur G. HuntPlant Cell Nov 2012, 24 (11) 4376-4388; DOI: https://doi.org/10.1105/tpc.112.096107
This work shows that poly(A) site choice is affected in 45% or more of all genes in an Arabidopsis thaliana mutant that lacks a core polyadenylation factor subunit and that a novel poly(A) signal exists that can function in the absence of the affected protein. These results provide new insight into mechanisms of alternative polyadenylation in plants.
Gangadharan, Anju
- Open AccessThe Coronatine Toxin of Pseudomonas syringae Is a Multifunctional Suppressor of Arabidopsis DefenseXueqing Geng, Jiye Cheng, Anju Gangadharan, David MackeyPlant Cell Nov 2012, 24 (11) 4763-4774; DOI: https://doi.org/10.1105/tpc.112.105312
The bacterial phytotoxin coronatine mimics a plant hormone, jasmonic acid, and thus antagonizes signaling by another plant hormone, salicylic acid, important for plant defense. This study demonstrates that coronatine also suppresses SA-independent host defense, including the production of defense-promoting indole glucosinolates and has a target other than the jasmonic acid receptor.
García, Irene
- You have accessRestricted AccessCysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in ArabidopsisConsolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia GotorPlant Cell Nov 2012, 24 (11) 4621-4634; DOI: https://doi.org/10.1105/tpc.112.105403
This article highlights the role of hydrogen sulfide as a relevant signaling molecule in plants, of comparable importance as described in animals. This study shows the regulatory role of sulfide generated by the cytosolic l-Cys desulfhydrase 1 enzyme on autophagy in eukaryotes.
Geerinck, Jan
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Geng, Xueqing
- Open AccessThe Coronatine Toxin of Pseudomonas syringae Is a Multifunctional Suppressor of Arabidopsis DefenseXueqing Geng, Jiye Cheng, Anju Gangadharan, David MackeyPlant Cell Nov 2012, 24 (11) 4763-4774; DOI: https://doi.org/10.1105/tpc.112.105312
The bacterial phytotoxin coronatine mimics a plant hormone, jasmonic acid, and thus antagonizes signaling by another plant hormone, salicylic acid, important for plant defense. This study demonstrates that coronatine also suppresses SA-independent host defense, including the production of defense-promoting indole glucosinolates and has a target other than the jasmonic acid receptor.
Goossens, Alain
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Goring, Daphne R.
- You have accessRestricted AccessThe ARC1 E3 Ligase Gene Is Frequently Deleted in Self-Compatible Brassicaceae Species and Has a Conserved Role in Arabidopsis lyrata Self-Pollen RejectionEmily Indriolo, Pirashaanthy Tharmapalan, Stephen I. Wright, Daphne R. GoringPlant Cell Nov 2012, 24 (11) 4607-4620; DOI: https://doi.org/10.1105/tpc.112.104943
The ARC1 E3 ubiquitin ligase was previously shown to be required for self-pollen rejection in Brassica, and this report shows that its function is conserved in other Brassicaceae species. ARC1 was found to be required for self-pollen rejection in Arabidopsis lyrata and was frequently deleted in genomes of Brassicaceae species that had lost this self-incompatibility trait.
Gotor, Cecilia
- You have accessRestricted AccessCysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in ArabidopsisConsolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia GotorPlant Cell Nov 2012, 24 (11) 4621-4634; DOI: https://doi.org/10.1105/tpc.112.105403
This article highlights the role of hydrogen sulfide as a relevant signaling molecule in plants, of comparable importance as described in animals. This study shows the regulatory role of sulfide generated by the cytosolic l-Cys desulfhydrase 1 enzyme on autophagy in eukaryotes.
Gregory, Brian D.
- Open AccessRegulatory Impact of RNA Secondary Structure across the Arabidopsis TranscriptomeFan Li, Qi Zheng, Lee E. Vandivier, Matthew R. Willmann, Ying Chen, Brian D. GregoryPlant Cell Nov 2012, 24 (11) 4346-4359; DOI: https://doi.org/10.1105/tpc.112.104232
This study employs a high-throughput, sequencing-based, structure-mapping approach to investigate RNA secondary structure throughout the Arabidopsis transcriptome. By combining these structural insights with a number of other RNA sequencing–based approaches, it provides a global assessment of RNA folding and its significant regulatory effects in a plant transcriptome.
Greiner, Andre
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Gu, Lianfeng
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Gu, Yangnan
- Open AccessThe KEEP ON GOING Protein of Arabidopsis Regulates Intracellular Protein Trafficking and Is Degraded during Fungal InfectionYangnan Gu, Roger W. InnesPlant Cell Nov 2012, 24 (11) 4717-4730; DOI: https://doi.org/10.1105/tpc.112.105254
The targeting of proteins to specific locations within a cell is a dynamic process that requires sophisticated sorting mechanisms. This work shows that Arabidopsis KEEP ON GOING plays a central role in regulating movement of proteins from the plasma membrane to the central vacuole and in the secretion of defense proteins to the extracellular space.
Guo, Xiuping
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
H
Hegemann, Peter
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Hofer, Julie
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Hofmann, Nancy R.
- You have accessRestricted AccessUbiquitination and Exocytosis in Plant ImmunityNancy R. HofmannPlant Cell Nov 2012, 24 (11) 4312; DOI: https://doi.org/10.1105/tpc.112.241111
Hohn, Barbara
- You have accessRestricted AccessIn Planta Somatic Homologous Recombination Assay Revisited: A Successful and Versatile, but Delicate ToolHolger Puchta, Barbara HohnPlant Cell Nov 2012, 24 (11) 4324-4331; DOI: https://doi.org/10.1105/tpc.112.101824
Hommelsheim, Carl Maximilian
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Howell, Stephen H.
- You have accessRestricted AccessDegradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in ArabidopsisYimo Liu, Junmarie Soto Burgos, Yan Deng, Renu Srivastava, Stephen H. Howell, Diane C. BasshamPlant Cell Nov 2012, 24 (11) 4635-4651; DOI: https://doi.org/10.1105/tpc.112.101535
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), cells activate an ER stress response to enable plants to tolerate these conditions. This work shows that one facet of this response is the activation of the autophagy pathway for degradation of ER fragments in the vacuole, which is regulated by the IRE1b splicing factor.
Huang, Xi
- Open AccessArabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B LightXi Huang, Xinhao Ouyang, Panyu Yang, On Sun Lau, Gang Li, Jigang Li, Haodong Chen, Xing Wang DengPlant Cell Nov 2012, 24 (11) 4590-4606; DOI: https://doi.org/10.1105/tpc.112.103994
In UV-B–induced photomorphogenesis in Arabidopsis, COP1 is a UV-B–inducible gene and FHY3 and HY5 directly activate COP1, dependent on UV-B, by binding to the COP1 promoter to ensure photomorphogenic UV-B signaling. The working mode of FHY3 and HY5 in UV-B–specific signaling is distinct from that in far-red light and circadian conditions.
Humphries, John A.
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
Hunt, Arthur G.
- Open AccessGenome-Wide Control of Polyadenylation Site Choice by CPSF30 in ArabidopsisPatrick E. Thomas, Xiaohui Wu, Man Liu, Bobby Gaffney, Guoli Ji, Qingshun Q. Li, Arthur G. HuntPlant Cell Nov 2012, 24 (11) 4376-4388; DOI: https://doi.org/10.1105/tpc.112.096107
This work shows that poly(A) site choice is affected in 45% or more of all genes in an Arabidopsis thaliana mutant that lacks a core polyadenylation factor subunit and that a novel poly(A) signal exists that can function in the absence of the affected protein. These results provide new insight into mechanisms of alternative polyadenylation in plants.
I
Ichimura, Kazuya
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Ikeda, Miho
- Open AccessA Triantagonistic Basic Helix-Loop-Helix System Regulates Cell Elongation in ArabidopsisMiho Ikeda, Sumire Fujiwara, Nobutaka Mitsuda, Masaru Ohme-TakagiPlant Cell Nov 2012, 24 (11) 4483-4497; DOI: https://doi.org/10.1105/tpc.112.105023
This work identifies three basic helix-loop-helix (bHLH) transcription factors that competitively regulate cell elongation. In this tri-antagonistic system, a negative regulatory bHLH interacts with and interferes with the function of an activator bHLH that activates genes for cell elongation. A third bHLH inactivates the negative bHLH; this double negative regulation activates cell elongation.
Indriolo, Emily
- You have accessRestricted AccessThe ARC1 E3 Ligase Gene Is Frequently Deleted in Self-Compatible Brassicaceae Species and Has a Conserved Role in Arabidopsis lyrata Self-Pollen RejectionEmily Indriolo, Pirashaanthy Tharmapalan, Stephen I. Wright, Daphne R. GoringPlant Cell Nov 2012, 24 (11) 4607-4620; DOI: https://doi.org/10.1105/tpc.112.104943
The ARC1 E3 ubiquitin ligase was previously shown to be required for self-pollen rejection in Brassica, and this report shows that its function is conserved in other Brassicaceae species. ARC1 was found to be required for self-pollen rejection in Arabidopsis lyrata and was frequently deleted in genomes of Brassicaceae species that had lost this self-incompatibility trait.
Innes, Roger W.
- Open AccessThe KEEP ON GOING Protein of Arabidopsis Regulates Intracellular Protein Trafficking and Is Degraded during Fungal InfectionYangnan Gu, Roger W. InnesPlant Cell Nov 2012, 24 (11) 4717-4730; DOI: https://doi.org/10.1105/tpc.112.105254
The targeting of proteins to specific locations within a cell is a dynamic process that requires sophisticated sorting mechanisms. This work shows that Arabidopsis KEEP ON GOING plays a central role in regulating movement of proteins from the plasma membrane to the central vacuole and in the secretion of defense proteins to the extracellular space.
J
Ji, Guoli
- Open AccessGenome-Wide Control of Polyadenylation Site Choice by CPSF30 in ArabidopsisPatrick E. Thomas, Xiaohui Wu, Man Liu, Bobby Gaffney, Guoli Ji, Qingshun Q. Li, Arthur G. HuntPlant Cell Nov 2012, 24 (11) 4376-4388; DOI: https://doi.org/10.1105/tpc.112.096107
This work shows that poly(A) site choice is affected in 45% or more of all genes in an Arabidopsis thaliana mutant that lacks a core polyadenylation factor subunit and that a novel poly(A) signal exists that can function in the absence of the affected protein. These results provide new insight into mechanisms of alternative polyadenylation in plants.
Jiang, Ling
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Jiang, Tao
- You have accessRestricted AccessXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in ArabidopsisXiao Fang Zhu, Yuan Zhi Shi, Gui Jie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu, Shao Jian ZhengPlant Cell Nov 2012, 24 (11) 4731-4747; DOI: https://doi.org/10.1105/tpc.112.106039
Hemicellulose can retain a large amount of Al. This study demonstrates that an important component of hemicellulose, xyloglucan, can bind Al, and knockout of XTH31 increases Al resistance in Arabidopsis by decreasing xyloglucan endotransglucosylase action and the accumulation of xyloglucan in hemicellulose, which in turn reduces the retention of Al in the cell wall, thus excluding Al from roots.
Johnny, Cassandra
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
Jung, Choonkyun
- You have accessRestricted AccessGenome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in ArabidopsisJun Liu, Choonkyun Jung, Jun Xu, Huan Wang, Shulin Deng, Lucia Bernad, Catalina Arenas-Huertero, Nam-Hai ChuaPlant Cell Nov 2012, 24 (11) 4333-4345; DOI: https://doi.org/10.1105/tpc.112.102855
This work identifies 6480 long intergenic noncoding RNAs in Arabidopsis, many of which show organ-specific and stress-responsive expression. The biogenesis of a group of long intergenic noncoding RNAs is coregulated by the RNA processing proteins SERRATE, CBP20, and CBP80.
K
Kahles, André
- You have accessRestricted AccessPolypyrimidine Tract Binding Protein Homologs from Arabidopsis Are Key Regulators of Alternative Splicing with Implications in Fundamental Developmental ProcessesChristina Rühl, Eva Stauffer, André Kahles, Gabriele Wagner, Gabriele Drechsel, Gunnar Rätsch, Andreas WachterPlant Cell Nov 2012, 24 (11) 4360-4375; DOI: https://doi.org/10.1105/tpc.112.103622
Alternative precursor mRNA splicing massively expands the transcriptome diversity in higher eukaryotes. In this report, we describe Arabidopsis Polypyrimidine tract binding protein homologs as critical components of the plant splicing code and provide links between regulated alternative splicing events and seed germination as well as flowering time control.
Karlova, Rumyana
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Kateriya, Suneel
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Kazachkov, Michael
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Khang, Chang Hyun
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Kong, Yingzhen
- You have accessRestricted AccessA Galacturonic Acid–Containing Xyloglucan Is Involved in Arabidopsis Root Hair Tip GrowthMaria J. Peña, Yingzhen Kong, William S. York, Malcolm A. O’NeillPlant Cell Nov 2012, 24 (11) 4511-4524; DOI: https://doi.org/10.1105/tpc.112.103390
A unique galacturonic acidic–containing xyloglucan was identified in Arabidopsis root hair cell walls and characterized using genetic, biochemical, and chemical methods. Plants with a null mutation in a gene encoding a GT47 glycosyltransferase synthesize xyloglucan that lacks galacturonic acid and have short root hairs. These findings highlight a key role for acidic xyloglucan in root hair growth.
Kreimer, Georg
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Kuo, Min-Hao
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
L
Lam, Hon-Ming
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Lau, On Sun
- Open AccessArabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B LightXi Huang, Xinhao Ouyang, Panyu Yang, On Sun Lau, Gang Li, Jigang Li, Haodong Chen, Xing Wang DengPlant Cell Nov 2012, 24 (11) 4590-4606; DOI: https://doi.org/10.1105/tpc.112.103994
In UV-B–induced photomorphogenesis in Arabidopsis, COP1 is a UV-B–inducible gene and FHY3 and HY5 directly activate COP1, dependent on UV-B, by binding to the COP1 promoter to ensure photomorphogenic UV-B signaling. The working mode of FHY3 and HY5 in UV-B–specific signaling is distinct from that in far-red light and circadian conditions.
Lei, Gui Jie
- You have accessRestricted AccessXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in ArabidopsisXiao Fang Zhu, Yuan Zhi Shi, Gui Jie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu, Shao Jian ZhengPlant Cell Nov 2012, 24 (11) 4731-4747; DOI: https://doi.org/10.1105/tpc.112.106039
Hemicellulose can retain a large amount of Al. This study demonstrates that an important component of hemicellulose, xyloglucan, can bind Al, and knockout of XTH31 increases Al resistance in Arabidopsis by decreasing xyloglucan endotransglucosylase action and the accumulation of xyloglucan in hemicellulose, which in turn reduces the retention of Al in the cell wall, thus excluding Al from roots.
Li, Fan
- Open AccessRegulatory Impact of RNA Secondary Structure across the Arabidopsis TranscriptomeFan Li, Qi Zheng, Lee E. Vandivier, Matthew R. Willmann, Ying Chen, Brian D. GregoryPlant Cell Nov 2012, 24 (11) 4346-4359; DOI: https://doi.org/10.1105/tpc.112.104232
This study employs a high-throughput, sequencing-based, structure-mapping approach to investigate RNA secondary structure throughout the Arabidopsis transcriptome. By combining these structural insights with a number of other RNA sequencing–based approaches, it provides a global assessment of RNA folding and its significant regulatory effects in a plant transcriptome.
Li, Gang
- Open AccessArabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B LightXi Huang, Xinhao Ouyang, Panyu Yang, On Sun Lau, Gang Li, Jigang Li, Haodong Chen, Xing Wang DengPlant Cell Nov 2012, 24 (11) 4590-4606; DOI: https://doi.org/10.1105/tpc.112.103994
In UV-B–induced photomorphogenesis in Arabidopsis, COP1 is a UV-B–inducible gene and FHY3 and HY5 directly activate COP1, dependent on UV-B, by binding to the COP1 promoter to ensure photomorphogenic UV-B signaling. The working mode of FHY3 and HY5 in UV-B–specific signaling is distinct from that in far-red light and circadian conditions.
Li, Jigang
- Open AccessArabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B LightXi Huang, Xinhao Ouyang, Panyu Yang, On Sun Lau, Gang Li, Jigang Li, Haodong Chen, Xing Wang DengPlant Cell Nov 2012, 24 (11) 4590-4606; DOI: https://doi.org/10.1105/tpc.112.103994
In UV-B–induced photomorphogenesis in Arabidopsis, COP1 is a UV-B–inducible gene and FHY3 and HY5 directly activate COP1, dependent on UV-B, by binding to the COP1 promoter to ensure photomorphogenic UV-B signaling. The working mode of FHY3 and HY5 in UV-B–specific signaling is distinct from that in far-red light and circadian conditions.
Li, Man-Wah
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Li, Qingshun Q.
- Open AccessGenome-Wide Control of Polyadenylation Site Choice by CPSF30 in ArabidopsisPatrick E. Thomas, Xiaohui Wu, Man Liu, Bobby Gaffney, Guoli Ji, Qingshun Q. Li, Arthur G. HuntPlant Cell Nov 2012, 24 (11) 4376-4388; DOI: https://doi.org/10.1105/tpc.112.096107
This work shows that poly(A) site choice is affected in 45% or more of all genes in an Arabidopsis thaliana mutant that lacks a core polyadenylation factor subunit and that a novel poly(A) signal exists that can function in the absence of the affected protein. These results provide new insight into mechanisms of alternative polyadenylation in plants.
Li, Xiaobo
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
Lin, Feng
- You have accessRestricted AccessPhosphatidic Acid Regulates Microtubule Organization by Interacting with MAP65-1 in Response to Salt Stress in ArabidopsisQun Zhang, Feng Lin, Tonglin Mao, Jianing Nie, Min Yan, Ming Yuan, Wenhua ZhangPlant Cell Nov 2012, 24 (11) 4555-4576; DOI: https://doi.org/10.1105/tpc.112.104182
A plant phospholipase D (PLDα1) is activated by salt stress, and the produced lipid phosphatidic acid (PA) binds to a microtubule-associated protein MAP65-1. The PA and MAP65-1 interaction is essential for the regulation of microtubule organization and salt tolerance. This finding couples lipid signaling to the cytoskeleton and reveals a lipid-mediated signaling pathway that responds to stress.
Liu, Bensheng
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
Liu, Jun
- You have accessRestricted AccessGenome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in ArabidopsisJun Liu, Choonkyun Jung, Jun Xu, Huan Wang, Shulin Deng, Lucia Bernad, Catalina Arenas-Huertero, Nam-Hai ChuaPlant Cell Nov 2012, 24 (11) 4333-4345; DOI: https://doi.org/10.1105/tpc.112.102855
This work identifies 6480 long intergenic noncoding RNAs in Arabidopsis, many of which show organ-specific and stress-responsive expression. The biogenesis of a group of long intergenic noncoding RNAs is coregulated by the RNA processing proteins SERRATE, CBP20, and CBP80.
Liu, Man
- Open AccessGenome-Wide Control of Polyadenylation Site Choice by CPSF30 in ArabidopsisPatrick E. Thomas, Xiaohui Wu, Man Liu, Bobby Gaffney, Guoli Ji, Qingshun Q. Li, Arthur G. HuntPlant Cell Nov 2012, 24 (11) 4376-4388; DOI: https://doi.org/10.1105/tpc.112.096107
This work shows that poly(A) site choice is affected in 45% or more of all genes in an Arabidopsis thaliana mutant that lacks a core polyadenylation factor subunit and that a novel poly(A) signal exists that can function in the absence of the affected protein. These results provide new insight into mechanisms of alternative polyadenylation in plants.
Liu, Xin
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Liu, Yimo
- You have accessRestricted AccessDegradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in ArabidopsisYimo Liu, Junmarie Soto Burgos, Yan Deng, Renu Srivastava, Stephen H. Howell, Diane C. BasshamPlant Cell Nov 2012, 24 (11) 4635-4651; DOI: https://doi.org/10.1105/tpc.112.101535
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), cells activate an ER stress response to enable plants to tolerate these conditions. This work shows that one facet of this response is the activation of the autophagy pathway for degradation of ER fragments in the vacuole, which is regulated by the IRE1b splicing factor.
Lu, Yinghong
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Lucent, Del
- You have accessRestricted AccessCrystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin HomeostasisThomas S. Peat, Christine Böttcher, Janet Newman, Del Lucent, Nathan Cowieson, Christopher DaviesPlant Cell Nov 2012, 24 (11) 4525-4538; DOI: https://doi.org/10.1105/tpc.112.102921
The crystal structure of an indole-3-acetic acid amido synthetase from Vitis vinifera involved in auxin homeostasis is presented. Residues likely to be involved in acyl group, amino acid, and ATP substrate binding have been identified, and this information provides a tool for designing new, effective auxins.
M
Ma, Jianxin
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Mach, Jennifer
- You have accessRestricted AccessOpen Wide! Exine Patterning and Aperture Formation in Arabidopsis PollenJennifer MachPlant Cell Nov 2012, 24 (11) 4311; DOI: https://doi.org/10.1105/tpc.112.241110
Mackey, David
- Open AccessThe Coronatine Toxin of Pseudomonas syringae Is a Multifunctional Suppressor of Arabidopsis DefenseXueqing Geng, Jiye Cheng, Anju Gangadharan, David MackeyPlant Cell Nov 2012, 24 (11) 4763-4774; DOI: https://doi.org/10.1105/tpc.112.105312
The bacterial phytotoxin coronatine mimics a plant hormone, jasmonic acid, and thus antagonizes signaling by another plant hormone, salicylic acid, important for plant defense. This study demonstrates that coronatine also suppresses SA-independent host defense, including the production of defense-promoting indole glucosinolates and has a target other than the jasmonic acid receptor.
Mao, Chuan Zao
- You have accessRestricted AccessXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in ArabidopsisXiao Fang Zhu, Yuan Zhi Shi, Gui Jie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu, Shao Jian ZhengPlant Cell Nov 2012, 24 (11) 4731-4747; DOI: https://doi.org/10.1105/tpc.112.106039
Hemicellulose can retain a large amount of Al. This study demonstrates that an important component of hemicellulose, xyloglucan, can bind Al, and knockout of XTH31 increases Al resistance in Arabidopsis by decreasing xyloglucan endotransglucosylase action and the accumulation of xyloglucan in hemicellulose, which in turn reduces the retention of Al in the cell wall, thus excluding Al from roots.
Mao, Tonglin
- You have accessRestricted AccessPhosphatidic Acid Regulates Microtubule Organization by Interacting with MAP65-1 in Response to Salt Stress in ArabidopsisQun Zhang, Feng Lin, Tonglin Mao, Jianing Nie, Min Yan, Ming Yuan, Wenhua ZhangPlant Cell Nov 2012, 24 (11) 4555-4576; DOI: https://doi.org/10.1105/tpc.112.104182
A plant phospholipase D (PLDα1) is activated by salt stress, and the produced lipid phosphatidic acid (PA) binds to a microtubule-associated protein MAP65-1. The PA and MAP65-1 interaction is essential for the regulation of microtubule organization and salt tolerance. This finding couples lipid signaling to the cytoskeleton and reveals a lipid-mediated signaling pathway that responds to stress.
McDowell, John M.
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Mely, Yves
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Meyer, Etienne
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Mitsuda, Nobutaka
- Open AccessA Triantagonistic Basic Helix-Loop-Helix System Regulates Cell Elongation in ArabidopsisMiho Ikeda, Sumire Fujiwara, Nobutaka Mitsuda, Masaru Ohme-TakagiPlant Cell Nov 2012, 24 (11) 4483-4497; DOI: https://doi.org/10.1105/tpc.112.105023
This work identifies three basic helix-loop-helix (bHLH) transcription factors that competitively regulate cell elongation. In this tri-antagonistic system, a negative regulatory bHLH interacts with and interferes with the function of an activator bHLH that activates genes for cell elongation. A third bHLH inactivates the negative bHLH; this double negative regulation activates cell elongation.
Moellering, Eric R.
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
Mondy, Samuel
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Moreno, Inmaculada
- You have accessRestricted AccessCysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in ArabidopsisConsolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia GotorPlant Cell Nov 2012, 24 (11) 4621-4634; DOI: https://doi.org/10.1105/tpc.112.105403
This article highlights the role of hydrogen sulfide as a relevant signaling molecule in plants, of comparable importance as described in animals. This study shows the regulatory role of sulfide generated by the cytosolic l-Cys desulfhydrase 1 enzyme on autophagy in eukaryotes.
Mutterer, Jerôme
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Mysore, Kirankumar S.
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
N
Neukam, Martin
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Newman, Janet
- You have accessRestricted AccessCrystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin HomeostasisThomas S. Peat, Christine Böttcher, Janet Newman, Del Lucent, Nathan Cowieson, Christopher DaviesPlant Cell Nov 2012, 24 (11) 4525-4538; DOI: https://doi.org/10.1105/tpc.112.102921
The crystal structure of an indole-3-acetic acid amido synthetase from Vitis vinifera involved in auxin homeostasis is presented. Residues likely to be involved in acyl group, amino acid, and ATP substrate binding have been identified, and this information provides a tool for designing new, effective auxins.
Nie, Jianing
- You have accessRestricted AccessPhosphatidic Acid Regulates Microtubule Organization by Interacting with MAP65-1 in Response to Salt Stress in ArabidopsisQun Zhang, Feng Lin, Tonglin Mao, Jianing Nie, Min Yan, Ming Yuan, Wenhua ZhangPlant Cell Nov 2012, 24 (11) 4555-4576; DOI: https://doi.org/10.1105/tpc.112.104182
A plant phospholipase D (PLDα1) is activated by salt stress, and the produced lipid phosphatidic acid (PA) binds to a microtubule-associated protein MAP65-1. The PA and MAP65-1 interaction is essential for the regulation of microtubule organization and salt tolerance. This finding couples lipid signaling to the cytoskeleton and reveals a lipid-mediated signaling pathway that responds to stress.
Ning, Yuese
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
O
O’Neill, Malcolm A.
- You have accessRestricted AccessA Galacturonic Acid–Containing Xyloglucan Is Involved in Arabidopsis Root Hair Tip GrowthMaria J. Peña, Yingzhen Kong, William S. York, Malcolm A. O’NeillPlant Cell Nov 2012, 24 (11) 4511-4524; DOI: https://doi.org/10.1105/tpc.112.103390
A unique galacturonic acidic–containing xyloglucan was identified in Arabidopsis root hair cell walls and characterized using genetic, biochemical, and chemical methods. Plants with a null mutation in a gene encoding a GT47 glycosyltransferase synthesize xyloglucan that lacks galacturonic acid and have short root hairs. These findings highlight a key role for acidic xyloglucan in root hair growth.
Ohme-Takagi, Masaru
- Open AccessA Triantagonistic Basic Helix-Loop-Helix System Regulates Cell Elongation in ArabidopsisMiho Ikeda, Sumire Fujiwara, Nobutaka Mitsuda, Masaru Ohme-TakagiPlant Cell Nov 2012, 24 (11) 4483-4497; DOI: https://doi.org/10.1105/tpc.112.105023
This work identifies three basic helix-loop-helix (bHLH) transcription factors that competitively regulate cell elongation. In this tri-antagonistic system, a negative regulatory bHLH interacts with and interferes with the function of an activator bHLH that activates genes for cell elongation. A third bHLH inactivates the negative bHLH; this double negative regulation activates cell elongation.
Olcay, Ahmet Can
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Ouyang, Xinhao
- Open AccessArabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B LightXi Huang, Xinhao Ouyang, Panyu Yang, On Sun Lau, Gang Li, Jigang Li, Haodong Chen, Xing Wang DengPlant Cell Nov 2012, 24 (11) 4590-4606; DOI: https://doi.org/10.1105/tpc.112.103994
In UV-B–induced photomorphogenesis in Arabidopsis, COP1 is a UV-B–inducible gene and FHY3 and HY5 directly activate COP1, dependent on UV-B, by binding to the COP1 promoter to ensure photomorphogenic UV-B signaling. The working mode of FHY3 and HY5 in UV-B–specific signaling is distinct from that in far-red light and circadian conditions.
P
Pan, Yuan Jiang
- You have accessRestricted AccessXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in ArabidopsisXiao Fang Zhu, Yuan Zhi Shi, Gui Jie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu, Shao Jian ZhengPlant Cell Nov 2012, 24 (11) 4731-4747; DOI: https://doi.org/10.1105/tpc.112.106039
Hemicellulose can retain a large amount of Al. This study demonstrates that an important component of hemicellulose, xyloglucan, can bind Al, and knockout of XTH31 increases Al resistance in Arabidopsis by decreasing xyloglucan endotransglucosylase action and the accumulation of xyloglucan in hemicellulose, which in turn reduces the retention of Al in the cell wall, thus excluding Al from roots.
Park, Chan-Ho
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Park, Yeri
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
Peat, Thomas S.
- You have accessRestricted AccessCrystal Structure of an Indole-3-Acetic Acid Amido Synthetase from Grapevine Involved in Auxin HomeostasisThomas S. Peat, Christine Böttcher, Janet Newman, Del Lucent, Nathan Cowieson, Christopher DaviesPlant Cell Nov 2012, 24 (11) 4525-4538; DOI: https://doi.org/10.1105/tpc.112.102921
The crystal structure of an indole-3-acetic acid amido synthetase from Vitis vinifera involved in auxin homeostasis is presented. Residues likely to be involved in acyl group, amino acid, and ATP substrate binding have been identified, and this information provides a tool for designing new, effective auxins.
Pecenkova, Tamara
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Pei, Weike
- Open AccessFIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin FringeHui Su, Jinsheng Zhu, Chao Cai, Weike Pei, Jiaojiao Wang, Huaijian Dong, Haiyun RenPlant Cell Nov 2012, 24 (11) 4539-4554; DOI: https://doi.org/10.1105/tpc.112.099358
Fimbrin family proteins have long been known as actin binding proteins that regulate the formation of higher-order actin filament structures. FIM1, a fimbrin homolog from Lilium longiflorum, is found to cross-link actin microfilaments into bundles in vitro and is demonstrated to play important roles in the maintenance of the actin fringe in pollen tubes.
Peña, Maria J.
- You have accessRestricted AccessA Galacturonic Acid–Containing Xyloglucan Is Involved in Arabidopsis Root Hair Tip GrowthMaria J. Peña, Yingzhen Kong, William S. York, Malcolm A. O’NeillPlant Cell Nov 2012, 24 (11) 4511-4524; DOI: https://doi.org/10.1105/tpc.112.103390
A unique galacturonic acidic–containing xyloglucan was identified in Arabidopsis root hair cell walls and characterized using genetic, biochemical, and chemical methods. Plants with a null mutation in a gene encoding a GT47 glycosyltransferase synthesize xyloglucan that lacks galacturonic acid and have short root hairs. These findings highlight a key role for acidic xyloglucan in root hair growth.
Pérez-Pérez, María Esther
- You have accessRestricted AccessCysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in ArabidopsisConsolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia GotorPlant Cell Nov 2012, 24 (11) 4621-4634; DOI: https://doi.org/10.1105/tpc.112.105403
This article highlights the role of hydrogen sulfide as a relevant signaling molecule in plants, of comparable importance as described in animals. This study shows the regulatory role of sulfide generated by the cytosolic l-Cys desulfhydrase 1 enzyme on autophagy in eukaryotes.
Puchta, Holger
- You have accessRestricted AccessIn Planta Somatic Homologous Recombination Assay Revisited: A Successful and Versatile, but Delicate ToolHolger Puchta, Barbara HohnPlant Cell Nov 2012, 24 (11) 4324-4331; DOI: https://doi.org/10.1105/tpc.112.101824
Putterill, Joanna
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Q
Qi, Xinpeng
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Qin, Ruizhen
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Qiu, Yang
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
R
Ratet, Pascal
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Rätsch, Gunnar
- You have accessRestricted AccessPolypyrimidine Tract Binding Protein Homologs from Arabidopsis Are Key Regulators of Alternative Splicing with Implications in Fundamental Developmental ProcessesChristina Rühl, Eva Stauffer, André Kahles, Gabriele Wagner, Gabriele Drechsel, Gunnar Rätsch, Andreas WachterPlant Cell Nov 2012, 24 (11) 4360-4375; DOI: https://doi.org/10.1105/tpc.112.103622
Alternative precursor mRNA splicing massively expands the transcriptome diversity in higher eukaryotes. In this report, we describe Arabidopsis Polypyrimidine tract binding protein homologs as critical components of the plant splicing code and provide links between regulated alternative splicing events and seed germination as well as flowering time control.
Ren, Haiyun
- Open AccessFIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin FringeHui Su, Jinsheng Zhu, Chao Cai, Weike Pei, Jiaojiao Wang, Huaijian Dong, Haiyun RenPlant Cell Nov 2012, 24 (11) 4539-4554; DOI: https://doi.org/10.1105/tpc.112.099358
Fimbrin family proteins have long been known as actin binding proteins that regulate the formation of higher-order actin filament structures. FIM1, a fimbrin homolog from Lilium longiflorum, is found to cross-link actin microfilaments into bundles in vitro and is demonstrated to play important roles in the maintenance of the actin fringe in pollen tubes.
Renault, Hugues
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Reuter, Patrick
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Richert, Ludovic
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Romero, Luis C.
- You have accessRestricted AccessCysteine-Generated Sulfide in the Cytosol Negatively Regulates Autophagy and Modulates the Transcriptional Profile in ArabidopsisConsolación Álvarez, Irene García, Inmaculada Moreno, María Esther Pérez-Pérez, José L. Crespo, Luis C. Romero, Cecilia GotorPlant Cell Nov 2012, 24 (11) 4621-4634; DOI: https://doi.org/10.1105/tpc.112.105403
This article highlights the role of hydrogen sulfide as a relevant signaling molecule in plants, of comparable importance as described in animals. This study shows the regulatory role of sulfide generated by the cytosolic l-Cys desulfhydrase 1 enzyme on autophagy in eukaryotes.
Rossetto, Priscilla de Barros
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Rottmann, Theresa
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Rühl, Christina
- You have accessRestricted AccessPolypyrimidine Tract Binding Protein Homologs from Arabidopsis Are Key Regulators of Alternative Splicing with Implications in Fundamental Developmental ProcessesChristina Rühl, Eva Stauffer, André Kahles, Gabriele Wagner, Gabriele Drechsel, Gunnar Rätsch, Andreas WachterPlant Cell Nov 2012, 24 (11) 4360-4375; DOI: https://doi.org/10.1105/tpc.112.103622
Alternative precursor mRNA splicing massively expands the transcriptome diversity in higher eukaryotes. In this report, we describe Arabidopsis Polypyrimidine tract binding protein homologs as critical components of the plant splicing code and provide links between regulated alternative splicing events and seed germination as well as flowering time control.
S
Schellwat, Jana
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Schmitt, Martine
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Sears, Barbara B.
- You have accessRestricted AccessA Galactoglycerolipid Lipase Is Required for Triacylglycerol Accumulation and Survival Following Nitrogen Deprivation in Chlamydomonas reinhardtiiXiaobo Li, Eric R. Moellering, Bensheng Liu, Cassandra Johnny, Marie Fedewa, Barbara B. Sears, Min-Hao Kuo, Christoph BenningPlant Cell Nov 2012, 24 (11) 4670-4686; DOI: https://doi.org/10.1105/tpc.112.105106
A mutant of Chlamydomonas reinhardtii with impaired oil accumulation is shown to be deficient in a lipase with specificity for newly assembled monogalactolipids. Passage of fatty acids synthesized in the chloroplast through a transient chloroplast membrane lipid pool into triacylglycerols is proposed. A role of oil biosynthesis for survival following nutrient deprivation is demonstrated.
Shamir, Ron
- You have accessRestricted AccessThe MORPH Algorithm: Ranking Candidate Genes for Membership in Arabidopsis and Tomato PathwaysOren Tzfadia, David Amar, Louis M.T. Bradbury, Eleanore T. Wurtzel, Ron ShamirPlant Cell Nov 2012, 24 (11) 4389-4406; DOI: https://doi.org/10.1105/tpc.112.104513
A fundamental challenge in plant biology is to discover the unknown connections within and between pathways and to associate previously unknown genes with specific biological processes. MORPH is a computational method for predicting genes that function in or regulate a biological pathway. MORPH fills pathway gaps and defines complex biological networks as shown for Arabidopsis and tomato.
She, Maoyun
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Shen, Wenyun
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Shen, Zhouxin
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
Shi, Yuan Zhi
- You have accessRestricted AccessXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in ArabidopsisXiao Fang Zhu, Yuan Zhi Shi, Gui Jie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu, Shao Jian ZhengPlant Cell Nov 2012, 24 (11) 4731-4747; DOI: https://doi.org/10.1105/tpc.112.106039
Hemicellulose can retain a large amount of Al. This study demonstrates that an important component of hemicellulose, xyloglucan, can bind Al, and knockout of XTH31 increases Al resistance in Arabidopsis by decreasing xyloglucan endotransglucosylase action and the accumulation of xyloglucan in hemicellulose, which in turn reduces the retention of Al in the cell wall, thus excluding Al from roots.
Shirasu, Ken
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Shirsekar, Gautam
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Smith, Laurie G.
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
Songkumarn, Pattavipha
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Srivastava, Renu
- You have accessRestricted AccessDegradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in ArabidopsisYimo Liu, Junmarie Soto Burgos, Yan Deng, Renu Srivastava, Stephen H. Howell, Diane C. BasshamPlant Cell Nov 2012, 24 (11) 4635-4651; DOI: https://doi.org/10.1105/tpc.112.101535
Upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), cells activate an ER stress response to enable plants to tolerate these conditions. This work shows that one facet of this response is the activation of the autophagy pathway for degradation of ER fragments in the vacuole, which is regulated by the IRE1b splicing factor.
Stauffer, Eva
- You have accessRestricted AccessPolypyrimidine Tract Binding Protein Homologs from Arabidopsis Are Key Regulators of Alternative Splicing with Implications in Fundamental Developmental ProcessesChristina Rühl, Eva Stauffer, André Kahles, Gabriele Wagner, Gabriele Drechsel, Gunnar Rätsch, Andreas WachterPlant Cell Nov 2012, 24 (11) 4360-4375; DOI: https://doi.org/10.1105/tpc.112.103622
Alternative precursor mRNA splicing massively expands the transcriptome diversity in higher eukaryotes. In this report, we describe Arabidopsis Polypyrimidine tract binding protein homologs as critical components of the plant splicing code and provide links between regulated alternative splicing events and seed germination as well as flowering time control.
Stegmann, Martin
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Stymne, Sten
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Su, Hui
- Open AccessFIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin FringeHui Su, Jinsheng Zhu, Chao Cai, Weike Pei, Jiaojiao Wang, Huaijian Dong, Haiyun RenPlant Cell Nov 2012, 24 (11) 4539-4554; DOI: https://doi.org/10.1105/tpc.112.099358
Fimbrin family proteins have long been known as actin binding proteins that regulate the formation of higher-order actin filament structures. FIM1, a fimbrin homolog from Lilium longiflorum, is found to cross-link actin microfilaments into bundles in vitro and is demonstrated to play important roles in the maintenance of the actin fringe in pollen tubes.
Sutimantanapi, Dena
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
Sylvester, Anne W.
- Open AccessIdentification of PAN2 by Quantitative Proteomics as a Leucine-Rich Repeat–Receptor-Like Kinase Acting Upstream of PAN1 to Polarize Cell Division in MaizeXiaoguo Zhang, Michelle Facette, John A. Humphries, Zhouxin Shen, Yeri Park, Dena Sutimantanapi, Anne W. Sylvester, Steven P. Briggs, Laurie G. SmithPlant Cell Nov 2012, 24 (11) 4577-4589; DOI: https://doi.org/10.1105/tpc.112.104125
PAN2 functions with PAN1, a Leu-rich repeat–receptor-like kinase (LRR-RLK) to polarize the divisions that form stomatal subsidiary cells in maize. Quantitative proteomics was used to identify PAN2 as a second LRR-RLK. PAN2 functions upstream of PAN1, potentially perceiving extracellular cues that initiate or amplify premitotic subsidiary mother cell polarity.
T
Tadege, Million
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Tharmapalan, Pirashaanthy
- You have accessRestricted AccessThe ARC1 E3 Ligase Gene Is Frequently Deleted in Self-Compatible Brassicaceae Species and Has a Conserved Role in Arabidopsis lyrata Self-Pollen RejectionEmily Indriolo, Pirashaanthy Tharmapalan, Stephen I. Wright, Daphne R. GoringPlant Cell Nov 2012, 24 (11) 4607-4620; DOI: https://doi.org/10.1105/tpc.112.104943
The ARC1 E3 ubiquitin ligase was previously shown to be required for self-pollen rejection in Brassica, and this report shows that its function is conserved in other Brassicaceae species. ARC1 was found to be required for self-pollen rejection in Arabidopsis lyrata and was frequently deleted in genomes of Brassicaceae species that had lost this self-incompatibility trait.
Thomas, Patrick E.
- Open AccessGenome-Wide Control of Polyadenylation Site Choice by CPSF30 in ArabidopsisPatrick E. Thomas, Xiaohui Wu, Man Liu, Bobby Gaffney, Guoli Ji, Qingshun Q. Li, Arthur G. HuntPlant Cell Nov 2012, 24 (11) 4376-4388; DOI: https://doi.org/10.1105/tpc.112.096107
This work shows that poly(A) site choice is affected in 45% or more of all genes in an Arabidopsis thaliana mutant that lacks a core polyadenylation factor subunit and that a novel poly(A) signal exists that can function in the absence of the affected protein. These results provide new insight into mechanisms of alternative polyadenylation in plants.
Thomas, Stefan
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Tian, Zhixi
- You have accessRestricted AccessGenome-Wide Characterization of Nonreference Transposons Reveals Evolutionary Propensities of Transposons in SoybeanZhixi Tian, Meixia Zhao, Maoyun She, Jianchang Du, Steven B. Cannon, Xin Liu, Xun Xu, Xinpeng Qi, Man-Wah Li, Hon-Ming Lam, Jianxin MaPlant Cell Nov 2012, 24 (11) 4422-4436; DOI: https://doi.org/10.1105/tpc.112.103630
The authors developed a strategy to identify putative transposon insertions that are present in a resequenced soybean population but absent in the soybean reference genome. Comparison of the distribution patterns of these elements and the patterns in the reference genome revealed evolutionary processes that reshaped the landscape of transposons after their integration in the host genome.
Tikhonovich, Igor
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Tikunov, Yury M.
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.
Trippens, Jessica
- You have accessRestricted AccessPhototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas reinhardtiiJessica Trippens, Andre Greiner, Jana Schellwat, Martin Neukam, Theresa Rottmann, Yinghong Lu, Suneel Kateriya, Peter Hegemann, Georg KreimerPlant Cell Nov 2012, 24 (11) 4687-4702; DOI: https://doi.org/10.1105/tpc.112.103523
This study shows that an eyespot is a dynamic organelle and that the blue light photoreceptor phototropin is involved in the regulation of eyespot size and level of channelrhodopsin 1, which is the primary photoreceptor for photo-movement responses. This work also describes that, in addition to the C-terminal kinase domain, the N-terminal photoreceptor domains have independent signaling functions.
Trujillo, Marco
- You have accessRestricted AccessThe Ubiquitin Ligase PUB22 Targets a Subunit of the Exocyst Complex Required for PAMP-Triggered Responses in ArabidopsisMartin Stegmann, Ryan G. Anderson, Kazuya Ichimura, Tamara Pecenkova, Patrick Reuter, Viktor Žárský, John M. McDowell, Ken Shirasu, Marco TrujilloPlant Cell Nov 2012, 24 (11) 4703-4716; DOI: https://doi.org/10.1105/tpc.112.104463
Exo70B2, a subunit of the exocyst complex, which is involved in exocytosis, is targeted for degradation by the ubiquitin ligase PUB22 upon activation of the immune system. This work also reveals that Exo70B2 is required for the activation of PAMP-triggered responses.
Tzfadia, Oren
- You have accessRestricted AccessThe MORPH Algorithm: Ranking Candidate Genes for Membership in Arabidopsis and Tomato PathwaysOren Tzfadia, David Amar, Louis M.T. Bradbury, Eleanore T. Wurtzel, Ron ShamirPlant Cell Nov 2012, 24 (11) 4389-4406; DOI: https://doi.org/10.1105/tpc.112.104513
A fundamental challenge in plant biology is to discover the unknown connections within and between pathways and to associate previously unknown genes with specific biological processes. MORPH is a computational method for predicting genes that function in or regulate a biological pathway. MORPH fills pathway gaps and defines complex biological networks as shown for Arabidopsis and tomato.
U
Ülker, Bekir
- You have accessRestricted AccessReevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter LinesBekir Ülker, Carl Maximilian Hommelsheim, Tobias Berson, Stefan Thomas, Balakumaran Chandrasekar, Ahmet Can Olcay, Kenneth Wayne Berendzen, Lamprinos FrantzeskakisPlant Cell Nov 2012, 24 (11) 4314-4323; DOI: https://doi.org/10.1105/tpc.112.100404
Ullmann, Pascaline
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
V
Valent, Barbara
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Vandivier, Lee E.
- Open AccessRegulatory Impact of RNA Secondary Structure across the Arabidopsis TranscriptomeFan Li, Qi Zheng, Lee E. Vandivier, Matthew R. Willmann, Ying Chen, Brian D. GregoryPlant Cell Nov 2012, 24 (11) 4346-4359; DOI: https://doi.org/10.1105/tpc.112.104232
This study employs a high-throughput, sequencing-based, structure-mapping approach to investigate RNA secondary structure throughout the Arabidopsis transcriptome. By combining these structural insights with a number of other RNA sequencing–based approaches, it provides a global assessment of RNA folding and its significant regulatory effects in a plant transcriptome.
W
Wachter, Andreas
- You have accessRestricted AccessPolypyrimidine Tract Binding Protein Homologs from Arabidopsis Are Key Regulators of Alternative Splicing with Implications in Fundamental Developmental ProcessesChristina Rühl, Eva Stauffer, André Kahles, Gabriele Wagner, Gabriele Drechsel, Gunnar Rätsch, Andreas WachterPlant Cell Nov 2012, 24 (11) 4360-4375; DOI: https://doi.org/10.1105/tpc.112.103622
Alternative precursor mRNA splicing massively expands the transcriptome diversity in higher eukaryotes. In this report, we describe Arabidopsis Polypyrimidine tract binding protein homologs as critical components of the plant splicing code and provide links between regulated alternative splicing events and seed germination as well as flowering time control.
Wagner, Gabriele
- You have accessRestricted AccessPolypyrimidine Tract Binding Protein Homologs from Arabidopsis Are Key Regulators of Alternative Splicing with Implications in Fundamental Developmental ProcessesChristina Rühl, Eva Stauffer, André Kahles, Gabriele Wagner, Gabriele Drechsel, Gunnar Rätsch, Andreas WachterPlant Cell Nov 2012, 24 (11) 4360-4375; DOI: https://doi.org/10.1105/tpc.112.103622
Alternative precursor mRNA splicing massively expands the transcriptome diversity in higher eukaryotes. In this report, we describe Arabidopsis Polypyrimidine tract binding protein homologs as critical components of the plant splicing code and provide links between regulated alternative splicing events and seed germination as well as flowering time control.
Wan, Jianmin
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Wang, Dan
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Wang, Guo-Liang
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Wang, Haiyang
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Wang, Huan
- You have accessRestricted AccessGenome-Wide Analysis Uncovers Regulation of Long Intergenic Noncoding RNAs in ArabidopsisJun Liu, Choonkyun Jung, Jun Xu, Huan Wang, Shulin Deng, Lucia Bernad, Catalina Arenas-Huertero, Nam-Hai ChuaPlant Cell Nov 2012, 24 (11) 4333-4345; DOI: https://doi.org/10.1105/tpc.112.102855
This work identifies 6480 long intergenic noncoding RNAs in Arabidopsis, many of which show organ-specific and stress-responsive expression. The biogenesis of a group of long intergenic noncoding RNAs is coregulated by the RNA processing proteins SERRATE, CBP20, and CBP80.
Wang, Jiaojiao
- Open AccessFIMBRIN1 Is Involved in Lily Pollen Tube Growth by Stabilizing the Actin FringeHui Su, Jinsheng Zhu, Chao Cai, Weike Pei, Jiaojiao Wang, Huaijian Dong, Haiyun RenPlant Cell Nov 2012, 24 (11) 4539-4554; DOI: https://doi.org/10.1105/tpc.112.099358
Fimbrin family proteins have long been known as actin binding proteins that regulate the formation of higher-order actin filament structures. FIM1, a fimbrin homolog from Lilium longiflorum, is found to cross-link actin microfilaments into bundles in vitro and is demonstrated to play important roles in the maintenance of the actin fringe in pollen tubes.
Wang, Jiu-Lin
- Open AccessIdentification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceLiguo Zhang, Zhijun Cheng, Ruizhen Qin, Yang Qiu, Jiu-Lin Wang, Xiekui Cui, Lianfeng Gu, Xin Zhang, Xiuping Guo, Dan Wang, Ling Jiang, Chuan-yin Wu, Haiyang Wang, Xiaofeng Cao, Jianmin WanPlant Cell Nov 2012, 24 (11) 4407-4421; DOI: https://doi.org/10.1105/tpc.112.102269
This work identifies an epi-allele of rice Fertilization Independent Endosperm1 (FIE1) with DNA hypomethylation, reduced H3 Lys 9 dimethylation, increased H3 Lys 4 trimethylation, ectopic FIE1 expression, and loss imprinting, plus altered H3 Lys 27 trimethylation and perturbed expression of hundreds of genes. This suggests a regulatory link among these epigenetic marks.
Wang, Liping
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Wang, Ruyi
- Open AccessThe Magnaporthe oryzae Effector AvrPiz-t Targets the RING E3 Ubiquitin Ligase APIP6 to Suppress Pathogen-Associated Molecular Pattern–Triggered Immunity in RiceChan-Ho Park, Songbiao Chen, Gautam Shirsekar, Bo Zhou, Chang Hyun Khang, Pattavipha Songkumarn, Ahmed J. Afzal, Yuese Ning, Ruyi Wang, Maria Bellizzi, Barbara Valent, Guo-Liang WangPlant Cell Nov 2012, 24 (11) 4748-4762; DOI: https://doi.org/10.1105/tpc.112.105429
This work shows that the Magnaporthe oryzae effector AvrPiz-t enters into rice cells to target the RING E3 ubiquitin ligase APIP6 for suppression of PAMP-triggered immunity in rice. It also describes that APIP6 degrades AvrPiz-t in planta and positively regulates basal defense to M. oryzae.
Wen, Jiangqi
- Open AccessNODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE GenesJean-Malo Couzigou, Vladimir Zhukov, Samuel Mondy, Ghada Abu el Heba, Viviane Cosson, T.H. Noel Ellis, Mike Ambrose, Jiangqi Wen, Million Tadege, Igor Tikhonovich, Kirankumar S. Mysore, Joanna Putterill, Julie Hofer, Alexei Y. Borisov, Pascal RatetPlant Cell Nov 2012, 24 (11) 4498-4510; DOI: https://doi.org/10.1105/tpc.112.103747
Medicago truncatula NOOT and Pisum sativum COCH were found to maintain nodule identity during symbiotic interactions with rhizobia and were identified as orthologs of Arabidopsis BLADE-ON-PETIOLE genes, which are involved in leaf and flower development.
Werck-Reichhart, Danièle
- Open AccessProtein–Protein and Protein–Membrane Associations in the Lignin PathwayJean-Etienne Bassard, Ludovic Richert, Jan Geerinck, Hugues Renault, Frédéric Duval, Pascaline Ullmann, Martine Schmitt, Etienne Meyer, Jerôme Mutterer, Wout Boerjan, Geert De Jaeger, Yves Mely, Alain Goossens, Danièle Werck-ReichhartPlant Cell Nov 2012, 24 (11) 4465-4482; DOI: https://doi.org/10.1105/tpc.112.102566
Analysis of the supramolecular organization of enzymes in the lignin pathway shows that cytochrome P450s oligomerize and move along with the very mobile plant endoplasmic reticulum. Their expression favors relocalization of their soluble partner proteins nearer the membrane and association of sequential enzymes in the pathway.
Weselake, Randall J.
- You have accessRestricted AccessMetabolic Interactions between the Lands Cycle and the Kennedy Pathway of Glycerolipid Synthesis in Arabidopsis Developing SeedsLiping Wang, Wenyun Shen, Michael Kazachkov, Guanqun Chen, Qilin Chen, Anders S. Carlsson, Sten Stymne, Randall J. Weselake, Jitao ZouPlant Cell Nov 2012, 24 (11) 4652-4669; DOI: https://doi.org/10.1105/tpc.112.104604
The Kennedy pathway and the Lands cycle are two principal metabolic modules of glycerolipid metabolism. This work examines the crosstalk of these two pathways and shows that loss of Lands cycle activity leads to an enhanced de novo phosphatidylcholine PC synthesis through the Kennedy pathway and PC turnover in Arabidopsis developing seeds.
Willmann, Matthew R.
- Open AccessRegulatory Impact of RNA Secondary Structure across the Arabidopsis TranscriptomeFan Li, Qi Zheng, Lee E. Vandivier, Matthew R. Willmann, Ying Chen, Brian D. GregoryPlant Cell Nov 2012, 24 (11) 4346-4359; DOI: https://doi.org/10.1105/tpc.112.104232
This study employs a high-throughput, sequencing-based, structure-mapping approach to investigate RNA secondary structure throughout the Arabidopsis transcriptome. By combining these structural insights with a number of other RNA sequencing–based approaches, it provides a global assessment of RNA folding and its significant regulatory effects in a plant transcriptome.
Wolters-Arts, Mieke
- You have accessRestricted AccessThe Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 Regulate Ethylene-Independent Aspects of Fruit RipeningMarian Bemer, Rumyana Karlova, Ana Rosa Ballester, Yury M. Tikunov, Arnaud G. Bovy, Mieke Wolters-Arts, Priscilla de Barros Rossetto, Gerco C. Angenent, Ruud A. de MaagdPlant Cell Nov 2012, 24 (11) 4437-4451; DOI: https://doi.org/10.1105/tpc.112.103283
Ripening of the tomato fruit is accompanied by an increase in ethylene production and involves color changes, altered sugar metabolism, tissue softening, and the synthesis of aroma volatiles. This study shows that the MADS domain transcription factors FUL1 and FUL2 play a role in the regulation of these ripening processes, but in an ethylene-independent manner.