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SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1

Rumen Ivanov, Tzvetina Brumbarova, Ailisa Blum, Anna-Maria Jantke, Claudia Fink-Straube, Petra Bauer
Rumen Ivanov
aInstitute of Botany, Heinrich-Heine University, D-40225 Duesseldorf, Germany
bCluster of Excellence on Plant Sciences, Heinrich-Heine University, D-40225 Duesseldorf, Germany
cDepartment of Biosciences-Plant Biology, Saarland University, D-66123 Saarbrücken, Germany
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  • For correspondence: rumen.ivanov@uni-duesseldorf.de
Tzvetina Brumbarova
aInstitute of Botany, Heinrich-Heine University, D-40225 Duesseldorf, Germany
bCluster of Excellence on Plant Sciences, Heinrich-Heine University, D-40225 Duesseldorf, Germany
cDepartment of Biosciences-Plant Biology, Saarland University, D-66123 Saarbrücken, Germany
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Ailisa Blum
cDepartment of Biosciences-Plant Biology, Saarland University, D-66123 Saarbrücken, Germany
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Anna-Maria Jantke
cDepartment of Biosciences-Plant Biology, Saarland University, D-66123 Saarbrücken, Germany
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Claudia Fink-Straube
dLeibniz Institute for New Materials, D-66123 Saarbrücken, Germany
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Petra Bauer
aInstitute of Botany, Heinrich-Heine University, D-40225 Duesseldorf, Germany
bCluster of Excellence on Plant Sciences, Heinrich-Heine University, D-40225 Duesseldorf, Germany
cDepartment of Biosciences-Plant Biology, Saarland University, D-66123 Saarbrücken, Germany
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Published March 2014. DOI: https://doi.org/10.1105/tpc.113.116244

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  • © 2014 American Society of Plant Biologists. All rights reserved.

Abstract

Dicotyledonous plants growing under limited iron availability initiate a response resulting in the solubilization, reduction, and uptake of soil iron. The protein factors responsible for these steps are transmembrane proteins, suggesting that the intracellular trafficking machinery may be involved in iron acquisition. In search for components involved in the regulation of Arabidopsis thaliana iron deficiency responses, we identified the members of the SORTING NEXIN (SNX) protein family. SNX loss-of-function plants display enhanced susceptibility to iron deficiency in comparison to the wild type. The absence of SNX led to reduced iron import efficiency into the root. SNX1 showed partial colocalization with the principal root iron importer IRON-REGULATED TRANSPORTER1 (IRT1). In SNX loss-of-function plants, IRT1 protein levels were decreased compared with the wild type due to enhanced IRT1 degradation. This resulted in diminished amounts of the IRT1 protein at the plasma membrane. snx mutants exhibited enhanced iron deficiency responses compared with the wild type, presumably due to the lower iron uptake through IRT1. Our results reveal a role of SNX1 for the correct trafficking of IRT1 and, thus, for modulating the activity of the iron uptake machinery.

  • Glossary

    PM
    plasma membrane
    bHLH
    basic helix-loop-helix
    Col-0
    Columbia-0
    TGN
    trans-Golgi network
    CHX
    cycloheximide
    • Received July 16, 2013.
    • Revised February 3, 2014.
    • Accepted February 10, 2014.
    • Published March 4, 2014.
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    SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1
    Rumen Ivanov, Tzvetina Brumbarova, Ailisa Blum, Anna-Maria Jantke, Claudia Fink-Straube, Petra Bauer
    The Plant Cell Mar 2014, 26 (3) 1294-1307; DOI: 10.1105/tpc.113.116244

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    SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1
    Rumen Ivanov, Tzvetina Brumbarova, Ailisa Blum, Anna-Maria Jantke, Claudia Fink-Straube, Petra Bauer
    The Plant Cell Mar 2014, 26 (3) 1294-1307; DOI: 10.1105/tpc.113.116244
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