Table of Contents
A
Akhunov, Eduard
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Akhunova, Alina
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Alcocer, Marcelo
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Altmann, Friedrich
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Altmann, Simone
- You have accessRestricted AccessArabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription FactorsAnna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel BäurlePlant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851
The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.
Amtmann, Anna
- Open AccessAnalysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional SignalsFabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna AmtmannPlant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101
Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.
Andreuzza, Sebastien
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
Armbruster, Ute
- Open AccessHigh-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNARu Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. JonikasPlant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099
In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.
Armengaud, Patrick
- Open AccessAnalysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional SignalsFabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna AmtmannPlant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101
Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.
B
Bai, Ling
- You have accessRestricted AccessA Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in ArabidopsisLing Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng SongPlant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586
Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.
Ballottari, Matteo
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Bano, S. Asma
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Bassi, Roberto
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Bäurle, Isabel
- You have accessRestricted AccessArabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription FactorsAnna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel BäurlePlant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851
The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.
Blaby, Ian K.
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Blifernez-Klassen, Olga
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Blum, Sean R.
- Open AccessHigh-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNARu Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. JonikasPlant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099
In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.
Boden, Scott A.
- You have accessRestricted AccessEARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T ExpressionScott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. SwainPlant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794
This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.
Botella, Miguel A.
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
Boulay, Clémence
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
Bowler, Chris
- Open AccessMethylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutumFeng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua HuPlant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982
The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.
Boyle, Nanette R.
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Brand, Luise H.
- Open AccessThe Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional ActivationSebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. ZeemanPlant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703
This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.
Braun, Karl-Heinz
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
Braun, Pascal
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
Bucher, Marcel
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
Buell, C. Robin
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
Buschmann, Henrik
- You have accessRestricted AccessActin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf TrichomesAdrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik BuschmannPlant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273
This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.
C
Casero, David
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Chai, Maofeng
- Open AccessSTM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatulaChuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu WangPlant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885
An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.
Chandler, Peter M.
- You have accessRestricted AccessEARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T ExpressionScott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. SwainPlant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794
This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.
Chelysheva, Liudmila
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Chen, Jie
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Chen, Mingjiao
- You have accessRestricted AccessThe Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther DevelopmentZhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi LiangPlant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745
This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.
Chen, Tong
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
Chen, Zhuo
- Open AccessMethylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutumFeng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua HuPlant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982
The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.
Cheng, Xiaofei
- Open AccessSTM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatulaChuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu WangPlant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885
An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.
Cheng, Xiaowei
- You have accessRestricted AccessThe Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther DevelopmentZhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi LiangPlant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745
This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.
Chèvre, Anne Marie
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Cho, Hyung-Taeg
- Open AccessFunctional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED ProteinsAnindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg ChoPlant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422
The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.
Comai, Luca
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Coriton, Olivier
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Courty, Pierre-Emmanuel
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
Cousins, Donna
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Cuñado, Nieves
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
D
D’Andrea, Cosimo
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Danku, John
- Open AccessAnalysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional SignalsFabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna AmtmannPlant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101
Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.
Davies, Noel W.
- You have accessRestricted AccessEARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T ExpressionScott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. SwainPlant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794
This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.
Ding, Zhiwen
- Open AccessABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in ArabidopsisJie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing ZhangPlant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986
The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.
Downie, J. Allan
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Dubcovsky, Jorge
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
E
Eber, Frédérique
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Eckardt, Nancy A.
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas: Reduce, Reuse, Recycle, and ReallocateNancy A. EckardtPlant Cell Apr 2014, 26 (4) 1379; DOI: https://doi.org/10.1105/tpc.114.126334
F
Fan, Lusheng
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
Findlay, Kim
- You have accessRestricted AccessActin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf TrichomesAdrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik BuschmannPlant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273
This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.
Franken, Philipp
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
Fu, Chunxiang
- Open AccessSTM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatulaChuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu WangPlant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885
An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.
Fu, Zhenzhen
- You have accessRestricted AccessThe Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther DevelopmentZhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi LiangPlant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745
This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.
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Gang, Spencer S.
- Open AccessHigh-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNARu Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. JonikasPlant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099
In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.
Ganguly, Anindya
- Open AccessFunctional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED ProteinsAnindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg ChoPlant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422
The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.
Gao, Lijie
- You have accessRestricted AccessA Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in ArabidopsisLing Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng SongPlant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586
Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.
Ge, Feng
- Open AccessMethylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutumFeng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua HuPlant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982
The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.
Gerlach, Nina
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
Go, Young Sam
- Open AccessArabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription FactorYoung Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung SuhPlant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307
This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.
Gong, Zhiyun
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
Goring, Daphne R.
- You have accessRestricted AccessThe ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in ArabidopsisEmily Indriolo, Darya Safavian, Daphne R. GoringPlant Cell Apr 2014, 26 (4) 1525-1543; DOI: https://doi.org/10.1105/tpc.114.122879
This report examines ARC1’s role in reconstituting the self-incompatibility trait in Arabidopsis thaliana and demonstrates an important role for ARC1 in promoting a strong and stable pollen rejection response when expressed with two other A. lyrata self-incompatibility factors. The expression of ARC1 conferred another A. lyrata trait for self-pollen avoidance, termed approach herkogamy.
Grandont, Laurie
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Grass, Josephine
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Grelon, Mathilde
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Grewe, Sabrina
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Grossman, Arthur R.
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
H
Han, Danxiang
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Han, Lu
- Open AccessSTM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatulaChuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu WangPlant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885
An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.
Hankamer, Ben
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Hao, Huaiqing
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
Hause, Bettina
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
He, Qihua
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
He, Yuke
- You have accessRestricted AccessHEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in ArabidopsisShuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke HePlant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883
Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.
Hemme, Dorothea
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Henry, Isabelle M.
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Hoffmann, Karen
- You have accessRestricted AccessArabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription FactorsAnna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel BäurlePlant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851
The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.
Hofmann, Nancy
- You have accessRestricted AccessMarked for Destruction: MANNOSIDASE4 and 5 Process N-Linked Glycans into ER-Associated Degradation TagsNancy HofmannPlant Cell Apr 2014, 26 (4) 1381; DOI: https://doi.org/10.1105/tpc.114.126870
Hu, Hanhua
- Open AccessMethylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutumFeng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua HuPlant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982
The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.
Hu, Qiang
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Huang, Shi
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Huang, Weichao
- Open AccessMethylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutumFeng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua HuPlant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982
The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.
Huteau, Virgine
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Hüttner, Silvia
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
I
Indriolo, Emily
- You have accessRestricted AccessThe ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in ArabidopsisEmily Indriolo, Darya Safavian, Daphne R. GoringPlant Cell Apr 2014, 26 (4) 1525-1543; DOI: https://doi.org/10.1105/tpc.114.122879
This report examines ARC1’s role in reconstituting the self-incompatibility trait in Arabidopsis thaliana and demonstrates an important role for ARC1 in promoting a strong and stable pollen rejection response when expressed with two other A. lyrata self-incompatibility factors. The expression of ARC1 conferred another A. lyrata trait for self-pollen avoidance, termed approach herkogamy.
J
Jarsch, Iris K.
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
Jenczewski, Eric
- You have accessRestricted AccessHomoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric JenczewskiPlant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788
Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.
Jia, Jing
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Jiang, Jiming
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
Jing, Xiaoyan
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Jonikas, Martin C.
- Open AccessHigh-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNARu Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. JonikasPlant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099
In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.
K
Kellermeier, Fabian
- Open AccessAnalysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional SignalsFabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna AmtmannPlant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101
Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.
Kesawat, Mahipal Singh
- Open AccessFunctional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED ProteinsAnindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg ChoPlant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422
The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.
Kim, Hae Jin
- Open AccessArabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription FactorYoung Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung SuhPlant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307
This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.
Kim, Hyojin
- Open AccessArabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription FactorYoung Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung SuhPlant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307
This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.
Kirilovsky, Diana
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
Koblížková, Andrea
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
Konrad, Sebastian S.A.
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
Krajinski, Franziska
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
Krasileva, Ksenia V.
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Krieger-Liszkay, Anja
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
Kropat, Janette
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Kruse, Olaf
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Kryvoruchko, Igor
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
L
Li, Guifen
- Open AccessSTM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatulaChuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu WangPlant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885
An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.
Li, Jing
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Li, Ruili
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
Li, Shuxia
- You have accessRestricted AccessHEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in ArabidopsisShuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke HePlant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883
Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.
Li, Xiaojuan
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
Li, Xiaorong
- You have accessRestricted AccessHEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in ArabidopsisShuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke HePlant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883
Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.
Li, Yantao
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Li, Zongyun
- You have accessRestricted AccessThe Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther DevelopmentZhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi LiangPlant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745
This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.
Liang, Wanqi
- You have accessRestricted AccessThe Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther DevelopmentZhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi LiangPlant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745
This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.
- Open AccessABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in ArabidopsisJie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing ZhangPlant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986
The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.
Lieberman, Meric C.
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Liebminger, Eva
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Lin, Jinxing
- You have accessRestricted AccessClathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in ArabidopsisHuaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing LinPlant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358
This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.
Liu, Chengwu
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Liu, Jinxin
- You have accessRestricted AccessHEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in ArabidopsisShuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke HePlant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883
Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.
Liu, Yu
- Open AccessSPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in RiceQundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping WuPlant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208
This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.
Liu, Zhongyuan
- You have accessRestricted AccessHEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in ArabidopsisShuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke HePlant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883
Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.
Lloyd, Clive W.
- You have accessRestricted AccessActin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf TrichomesAdrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik BuschmannPlant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273
This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.
López-Igual, Rocío
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
Luginbühl, Leonie
- Open AccessThe Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional ActivationSebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. ZeemanPlant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703
This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.
Lv, Qundan
- Open AccessSPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in RiceQundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping WuPlant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208
This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.
M
Ma, Xiaonan
- You have accessRestricted AccessA Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in ArabidopsisLing Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng SongPlant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586
Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.
Macas, Jiří
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
Mach, Jennifer
- You have accessRestricted AccessOrange Carotenoid Protein Quenches Excess Energy and Singlet OxygenJennifer MachPlant Cell Apr 2014, 26 (4) 1380; DOI: https://doi.org/10.1105/tpc.114.126581
Mach, Lukas
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Mao, Chuanzao
- Open AccessSPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in RiceQundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping WuPlant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208
This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.
Maresch, Daniel
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Merchant, Sabeeha S.
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Mettler, Tabea
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Miao, Yuchen
- You have accessRestricted AccessA Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in ArabidopsisLing Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng SongPlant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586
Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.
Miller, Anthony J.
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Moseley, Jeffrey L.
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Mühlhaus, Timo
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Murray, Jeremy D.
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Mussgnug, Jan H.
- You have accessRestricted AccessLight-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtiiSabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf KrusePlant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198
This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.
Mysore, Kirankumar S.
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
N
Nagalakshmi, Ugrappa
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Ngo, Kathie J.
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Ning, Kang
- Open AccessChoreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in MicroalgaeJing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian XuPlant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418
To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.
Novák, Petr
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
O
Oldroyd, Giles E.D.
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Oliveira, Ludmila
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
Ott, Thomas
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
P
Palanivelu, Ravishankar
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
Panoli, Aneesh
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
Pant, Bikram Datt
- You have accessRestricted AccessArabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription FactorsAnna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel BäurlePlant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851
The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.
Park, Minho
- Open AccessFunctional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED ProteinsAnindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg ChoPlant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422
The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.
Patena, Weronika
- Open AccessHigh-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNARu Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. JonikasPlant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099
In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.
Pellegrini, Matteo
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Perreau, François
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
Q
Qin, Yuan
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
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Ratet, Pascal
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Ross, John J.
- You have accessRestricted AccessEARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T ExpressionScott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. SwainPlant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794
This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.
S
Safavian, Darya
- You have accessRestricted AccessThe ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in ArabidopsisEmily Indriolo, Darya Safavian, Daphne R. GoringPlant Cell Apr 2014, 26 (4) 1525-1543; DOI: https://doi.org/10.1105/tpc.114.122879
This report examines ARC1’s role in reconstituting the self-incompatibility trait in Arabidopsis thaliana and demonstrates an important role for ARC1 in promoting a strong and stable pollen rejection response when expressed with two other A. lyrata self-incompatibility factors. The expression of ARC1 conferred another A. lyrata trait for self-pollen avoidance, termed approach herkogamy.
Salt, David E.
- Open AccessAnalysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional SignalsFabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna AmtmannPlant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101
Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.
Sambade, Adrian
- You have accessRestricted AccessActin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf TrichomesAdrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik BuschmannPlant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273
This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.
Schäffner, Anton R.
- You have accessRestricted AccessActin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf TrichomesAdrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik BuschmannPlant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273
This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.
Scheible, Wolf-Rüdiger
- You have accessRestricted AccessArabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription FactorsAnna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel BäurlePlant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851
The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.
Schmollinger, Stefan
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Schoberer, Jennifer
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Schreiber, Lukas
- Open AccessABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in ArabidopsisJie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing ZhangPlant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986
The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.
Schroda, Michael
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Schultze, Michael
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Seditas, Triona J.
- Open AccessAnalysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional SignalsFabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna AmtmannPlant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101
Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.
Sedoud, Arezki
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
Shi, Jianxin
- Open AccessABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in ArabidopsisJie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing ZhangPlant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986
The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.
Shi, Jing
- Open AccessSPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in RiceQundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping WuPlant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208
This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.
Siddiqi, Imran
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
Sieh, Daniela
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
Šimková, Klára
- Open AccessThe Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional ActivationSebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. ZeemanPlant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703
This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.
Skaggs, Megan I.
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
Smith, Steven
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
Sommer, Frederik
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Song, Chun-Peng
- You have accessRestricted AccessA Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in ArabidopsisLing Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng SongPlant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586
Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.
Song, Shufei
- You have accessRestricted AccessA Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in ArabidopsisLing Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng SongPlant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586
Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.
Soyk, Sebastian
- Open AccessThe Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional ActivationSebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. ZeemanPlant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703
This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.
Stief, Anna
- You have accessRestricted AccessArabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription FactorsAnna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel BäurlePlant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851
The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.
Stitt, Mark
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Strasser, Richard
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Stratil, Thomas F.
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
Strenkert, Daniela
- You have accessRestricted AccessNitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic MetabolismStefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. MerchantPlant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523
This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.
Suh, Mi Chung
- Open AccessArabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription FactorYoung Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung SuhPlant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307
This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.
Swain, Steve M.
- You have accessRestricted AccessEARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T ExpressionScott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. SwainPlant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794
This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.
Szymanski, Witold
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
T
Tadege, Million
- Open AccessA H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatulaErtao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael SchultzePlant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527
Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.
Tai, Thomas H.
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Tang, Yuhong
- Open AccessSTM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatulaChuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu WangPlant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885
An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.
Torres, Giovana A.
- You have accessRestricted AccessBoom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with CentromeresHaiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming JiangPlant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877
This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.
Trevaskis, Ben
- You have accessRestricted AccessEARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T ExpressionScott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. SwainPlant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794
This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.
Tsukamoto, Tatsuya
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar PalaniveluPlant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576
This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.
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Udvardi, Michael
- Open AccessThe H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal SymbiosisFranziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina HausePlant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436
A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.
Urbanus, Susan L.
- You have accessRestricted AccessPlasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamianaIris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas OttPlant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446
The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.
ur Rehman, Ateeq
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
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Vasquez-Gross, Hans
- Open AccessEfficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation SequencingIsabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca ComaiPlant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590
The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.
Vass, Imre
- You have accessRestricted AccessThe Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen QuencherArezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana KirilovskyPlant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802
This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.
Vaughan, Cara K.
- Open AccessThe Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional ActivationSebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. ZeemanPlant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703
This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.
Vavra, Ulrike
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Veit, Christiane
- Open AccessArabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded GlycoproteinsSilvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard StrasserPlant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216
Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.
Vizcay-Barrena, Gema
- Open AccessABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in ArabidopsisJie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing ZhangPlant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986
The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.
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Wallace, Kirsten N.
- You have accessRestricted AccessACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in ArabidopsisYuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith,