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Table of Contents

The Plant Cell Online: 26 (4)
Apr 2014
  • A
  • B
  • C
  • D
  • E
  • F
  • G
  • H
  • I
  • J
  • K
  • L
  • M
  • N
  • O
  • P
  • Q
  • R
  • S
  • T
  • U
  • V
  • W
  • X
  • Y
  • Z

A

  1. Akhunov, Eduard

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  2. Akhunova, Alina

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  3. Alcocer, Marcelo

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  4. Altmann, Friedrich

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  5. Altmann, Simone

    1. <em>Arabidopsis miR156</em> Regulates Tolerance to Recurring Environmental Stress through <em>SPL</em> Transcription Factors
      You have accessRestricted Access
      Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors
      Anna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel Bäurle
      Plant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851

      The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.

  6. Amtmann, Anna

    1. Analysis of the Root System Architecture of <em>Arabidopsis</em> Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Open Access
      Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Fabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna Amtmann
      Plant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101

      Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.

  7. Andreuzza, Sebastien

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

  8. Armbruster, Ute

    1. High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Open Access
      High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Ru Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. Jonikas
      Plant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099

      In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.

  9. Armengaud, Patrick

    1. Analysis of the Root System Architecture of <em>Arabidopsis</em> Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Open Access
      Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Fabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna Amtmann
      Plant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101

      Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.

B

  1. Bai, Ling

    1. A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in <em>Arabidopsis</em>
      You have accessRestricted Access
      A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in Arabidopsis
      Ling Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng Song
      Plant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586

      Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.

  2. Ballottari, Matteo

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  3. Bano, S. Asma

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  4. Bassi, Roberto

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  5. Bäurle, Isabel

    1. <em>Arabidopsis miR156</em> Regulates Tolerance to Recurring Environmental Stress through <em>SPL</em> Transcription Factors
      You have accessRestricted Access
      Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors
      Anna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel Bäurle
      Plant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851

      The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.

  6. Blaby, Ian K.

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  7. Blifernez-Klassen, Olga

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  8. Blum, Sean R.

    1. High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Open Access
      High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Ru Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. Jonikas
      Plant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099

      In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.

  9. Boden, Scott A.

    1. <em>EARLY FLOWERING3</em> Regulates Flowering in Spring Barley by Mediating Gibberellin Production and <em>FLOWERING LOCUS T</em> Expression
      You have accessRestricted Access
      EARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T Expression
      Scott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. Swain
      Plant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794

      This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.

  10. Botella, Miguel A.

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  11. Boulay, Clémence

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

  12. Bowler, Chris

    1. Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom <em>Phaeodactylum tricornutum</em>
      Open Access
      Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
      Feng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua Hu
      Plant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982

      The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.

  13. Boyle, Nanette R.

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  14. Brand, Luise H.

    1. The Enzyme-Like Domain of <em>Arabidopsis</em> Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Open Access
      The Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Sebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. Zeeman
      Plant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703

      This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.

  15. Braun, Karl-Heinz

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

  16. Braun, Pascal

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

  17. Bucher, Marcel

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  18. Buell, C. Robin

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

  19. Buschmann, Henrik

    1. Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of <em>Arabidopsis</em> Leaf Trichomes
      You have accessRestricted Access
      Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf Trichomes
      Adrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik Buschmann
      Plant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273

      This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.

C

  1. Casero, David

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  2. Chai, Maofeng

    1. STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in <em>Medicago truncatula</em>
      Open Access
      STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
      Chuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu Wang
      Plant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885

      An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.

  3. Chandler, Peter M.

    1. <em>EARLY FLOWERING3</em> Regulates Flowering in Spring Barley by Mediating Gibberellin Production and <em>FLOWERING LOCUS T</em> Expression
      You have accessRestricted Access
      EARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T Expression
      Scott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. Swain
      Plant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794

      This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.

  4. Chelysheva, Liudmila

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  5. Chen, Jie

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  6. Chen, Mingjiao

    1. The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      You have accessRestricted Access
      The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      Zhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi Liang
      Plant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745

      This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

  7. Chen, Tong

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  8. Chen, Zhuo

    1. Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom <em>Phaeodactylum tricornutum</em>
      Open Access
      Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
      Feng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua Hu
      Plant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982

      The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.

  9. Cheng, Xiaofei

    1. STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in <em>Medicago truncatula</em>
      Open Access
      STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
      Chuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu Wang
      Plant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885

      An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.

  10. Cheng, Xiaowei

    1. The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      You have accessRestricted Access
      The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      Zhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi Liang
      Plant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745

      This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

  11. Chèvre, Anne Marie

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  12. Cho, Hyung-Taeg

    1. Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of <em>Arabidopsis</em> PIN-FORMED Proteins
      Open Access
      Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED Proteins
      Anindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg Cho
      Plant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422

      The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.

  13. Comai, Luca

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  14. Coriton, Olivier

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  15. Courty, Pierre-Emmanuel

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  16. Cousins, Donna

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  17. Cuñado, Nieves

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

D

  1. D’Andrea, Cosimo

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  2. Danku, John

    1. Analysis of the Root System Architecture of <em>Arabidopsis</em> Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Open Access
      Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Fabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna Amtmann
      Plant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101

      Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.

  3. Davies, Noel W.

    1. <em>EARLY FLOWERING3</em> Regulates Flowering in Spring Barley by Mediating Gibberellin Production and <em>FLOWERING LOCUS T</em> Expression
      You have accessRestricted Access
      EARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T Expression
      Scott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. Swain
      Plant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794

      This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.

  4. Ding, Zhiwen

    1. <em>ABORTED MICROSPORES</em> Acts as a Master Regulator of Pollen Wall Formation in <em>Arabidopsis</em>
      Open Access
      ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis
      Jie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing Zhang
      Plant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986

      The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.

  5. Downie, J. Allan

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  6. Dubcovsky, Jorge

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

E

  1. Eber, Frédérique

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  2. Eckardt, Nancy A.

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em>: Reduce, Reuse, Recycle, and Reallocate
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas: Reduce, Reuse, Recycle, and Reallocate
      Nancy A. Eckardt
      Plant Cell Apr 2014, 26 (4) 1379; DOI: https://doi.org/10.1105/tpc.114.126334

F

  1. Fan, Lusheng

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  2. Findlay, Kim

    1. Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of <em>Arabidopsis</em> Leaf Trichomes
      You have accessRestricted Access
      Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf Trichomes
      Adrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik Buschmann
      Plant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273

      This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.

  3. Franken, Philipp

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  4. Fu, Chunxiang

    1. STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in <em>Medicago truncatula</em>
      Open Access
      STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
      Chuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu Wang
      Plant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885

      An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.

  5. Fu, Zhenzhen

    1. The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      You have accessRestricted Access
      The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      Zhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi Liang
      Plant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745

      This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

G

  1. Gang, Spencer S.

    1. High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Open Access
      High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Ru Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. Jonikas
      Plant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099

      In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.

  2. Ganguly, Anindya

    1. Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of <em>Arabidopsis</em> PIN-FORMED Proteins
      Open Access
      Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED Proteins
      Anindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg Cho
      Plant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422

      The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.

  3. Gao, Lijie

    1. A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in <em>Arabidopsis</em>
      You have accessRestricted Access
      A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in Arabidopsis
      Ling Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng Song
      Plant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586

      Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.

  4. Ge, Feng

    1. Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom <em>Phaeodactylum tricornutum</em>
      Open Access
      Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
      Feng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua Hu
      Plant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982

      The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.

  5. Gerlach, Nina

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  6. Go, Young Sam

    1. <em>Arabidopsis</em> Cuticular Wax Biosynthesis Is Negatively Regulated by the <em>DEWAX</em> Gene Encoding an AP2/ERF-Type Transcription Factor
      Open Access
      Arabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription Factor
      Young Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung Suh
      Plant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307

      This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.

  7. Gong, Zhiyun

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

  8. Goring, Daphne R.

    1. The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in <em>Arabidopsis</em>
      You have accessRestricted Access
      The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in Arabidopsis
      Emily Indriolo, Darya Safavian, Daphne R. Goring
      Plant Cell Apr 2014, 26 (4) 1525-1543; DOI: https://doi.org/10.1105/tpc.114.122879

      This report examines ARC1’s role in reconstituting the self-incompatibility trait in Arabidopsis thaliana and demonstrates an important role for ARC1 in promoting a strong and stable pollen rejection response when expressed with two other A. lyrata self-incompatibility factors. The expression of ARC1 conferred another A. lyrata trait for self-pollen avoidance, termed approach herkogamy.

  9. Grandont, Laurie

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  10. Grass, Josephine

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  11. Grelon, Mathilde

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  12. Grewe, Sabrina

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  13. Grossman, Arthur R.

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

H

  1. Han, Danxiang

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  2. Han, Lu

    1. STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in <em>Medicago truncatula</em>
      Open Access
      STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
      Chuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu Wang
      Plant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885

      An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.

  3. Hankamer, Ben

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  4. Hao, Huaiqing

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  5. Hause, Bettina

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  6. He, Qihua

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  7. He, Yuke

    1. <em>HEAT-INDUCED TAS1 TARGET1</em> Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in <em>Arabidopsis</em>
      You have accessRestricted Access
      HEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in Arabidopsis
      Shuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke He
      Plant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883

      Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.

  8. Hemme, Dorothea

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  9. Henry, Isabelle M.

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  10. Hoffmann, Karen

    1. <em>Arabidopsis miR156</em> Regulates Tolerance to Recurring Environmental Stress through <em>SPL</em> Transcription Factors
      You have accessRestricted Access
      Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors
      Anna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel Bäurle
      Plant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851

      The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.

  11. Hofmann, Nancy

    1. Marked for Destruction: MANNOSIDASE4 and 5 Process <em>N</em>-Linked Glycans into ER-Associated Degradation Tags
      You have accessRestricted Access
      Marked for Destruction: MANNOSIDASE4 and 5 Process N-Linked Glycans into ER-Associated Degradation Tags
      Nancy Hofmann
      Plant Cell Apr 2014, 26 (4) 1381; DOI: https://doi.org/10.1105/tpc.114.126870
  12. Hu, Hanhua

    1. Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom <em>Phaeodactylum tricornutum</em>
      Open Access
      Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
      Feng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua Hu
      Plant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982

      The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.

  13. Hu, Qiang

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  14. Huang, Shi

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  15. Huang, Weichao

    1. Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom <em>Phaeodactylum tricornutum</em>
      Open Access
      Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
      Feng Ge, Weichao Huang, Zhuo Chen, Chunye Zhang, Qian Xiong, Chris Bowler, Juan Yang, Jin Xu, Hanhua Hu
      Plant Cell Apr 2014, 26 (4) 1681-1697; DOI: https://doi.org/10.1105/tpc.114.124982

      The marine diatom Phaeodactylum tricornutum stores carbon and energy in the form of lipids, predominantly triacylglycerols (TAGs), making it an attractive system for biodiesel production, but the mechanism underlying TAG accumulation is unclear. Using label-free quantitative proteomics and functional analysis, this study shows that the carboxylase MCC2 regulates TAG accumulation in this model diatom.

  16. Huteau, Virgine

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  17. Hüttner, Silvia

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

I

  1. Indriolo, Emily

    1. The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in <em>Arabidopsis</em>
      You have accessRestricted Access
      The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in Arabidopsis
      Emily Indriolo, Darya Safavian, Daphne R. Goring
      Plant Cell Apr 2014, 26 (4) 1525-1543; DOI: https://doi.org/10.1105/tpc.114.122879

      This report examines ARC1’s role in reconstituting the self-incompatibility trait in Arabidopsis thaliana and demonstrates an important role for ARC1 in promoting a strong and stable pollen rejection response when expressed with two other A. lyrata self-incompatibility factors. The expression of ARC1 conferred another A. lyrata trait for self-pollen avoidance, termed approach herkogamy.

J

  1. Jarsch, Iris K.

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

  2. Jenczewski, Eric

    1. Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in <em>Brassica napus</em>: Ploidy Does Matter!
      You have accessRestricted Access
      Homoeologous Chromosome Sorting and Progression of Meiotic Recombination in Brassica napus: Ploidy Does Matter!
      Laurie Grandont, Nieves Cuñado, Olivier Coriton, Virgine Huteau, Frédérique Eber, Anne Marie Chèvre, Mathilde Grelon, Liudmila Chelysheva, Eric Jenczewski
      Plant Cell Apr 2014, 26 (4) 1448-1463; DOI: https://doi.org/10.1105/tpc.114.122788

      Comparisons of meiosis in near-isogenic allohaploid and euploid lines of B. napus reveal that the mechanism that promotes efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, in B. napus, chromosome sorting depends on context.

  3. Jia, Jing

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  4. Jiang, Jiming

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

  5. Jing, Xiaoyan

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  6. Jonikas, Martin C.

    1. High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Open Access
      High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Ru Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. Jonikas
      Plant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099

      In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.

K

  1. Kellermeier, Fabian

    1. Analysis of the Root System Architecture of <em>Arabidopsis</em> Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Open Access
      Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Fabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna Amtmann
      Plant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101

      Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.

  2. Kesawat, Mahipal Singh

    1. Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of <em>Arabidopsis</em> PIN-FORMED Proteins
      Open Access
      Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED Proteins
      Anindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg Cho
      Plant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422

      The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.

  3. Kim, Hae Jin

    1. <em>Arabidopsis</em> Cuticular Wax Biosynthesis Is Negatively Regulated by the <em>DEWAX</em> Gene Encoding an AP2/ERF-Type Transcription Factor
      Open Access
      Arabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription Factor
      Young Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung Suh
      Plant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307

      This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.

  4. Kim, Hyojin

    1. <em>Arabidopsis</em> Cuticular Wax Biosynthesis Is Negatively Regulated by the <em>DEWAX</em> Gene Encoding an AP2/ERF-Type Transcription Factor
      Open Access
      Arabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription Factor
      Young Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung Suh
      Plant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307

      This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.

  5. Kirilovsky, Diana

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

  6. Koblížková, Andrea

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

  7. Konrad, Sebastian S.A.

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

  8. Krajinski, Franziska

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  9. Krasileva, Ksenia V.

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  10. Krieger-Liszkay, Anja

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

  11. Kropat, Janette

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  12. Kruse, Olaf

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  13. Kryvoruchko, Igor

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

L

  1. Li, Guifen

    1. STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in <em>Medicago truncatula</em>
      Open Access
      STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
      Chuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu Wang
      Plant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885

      An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.

  2. Li, Jing

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  3. Li, Ruili

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  4. Li, Shuxia

    1. <em>HEAT-INDUCED TAS1 TARGET1</em> Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in <em>Arabidopsis</em>
      You have accessRestricted Access
      HEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in Arabidopsis
      Shuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke He
      Plant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883

      Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.

  5. Li, Xiaojuan

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  6. Li, Xiaorong

    1. <em>HEAT-INDUCED TAS1 TARGET1</em> Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in <em>Arabidopsis</em>
      You have accessRestricted Access
      HEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in Arabidopsis
      Shuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke He
      Plant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883

      Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.

  7. Li, Yantao

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  8. Li, Zongyun

    1. The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      You have accessRestricted Access
      The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      Zhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi Liang
      Plant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745

      This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

  9. Liang, Wanqi

    1. The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      You have accessRestricted Access
      The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
      Zhenzhen Fu, Jing Yu, Xiaowei Cheng, Xu Zong, Jie Xu, Mingjiao Chen, Zongyun Li, Dabing Zhang, Wanqi Liang
      Plant Cell Apr 2014, 26 (4) 1512-1524; DOI: https://doi.org/10.1105/tpc.114.123745

      This report identifies the rice bHLH protein TIP2 as a key switch for the meristemoid transition and cell differentiation during early anther development. TIP2 directly triggers the expression of the developmental regulators TDR and EAT1 and interacts with TDR, forming a central cascade regulating differentiation, morphogenesis, and degradation of anther somatic cell layers.

    2. <em>ABORTED MICROSPORES</em> Acts as a Master Regulator of Pollen Wall Formation in <em>Arabidopsis</em>
      Open Access
      ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis
      Jie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing Zhang
      Plant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986

      The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.

  10. Lieberman, Meric C.

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  11. Liebminger, Eva

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  12. Lin, Jinxing

    1. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in <em>Arabidopsis</em>
      You have accessRestricted Access
      Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis
      Huaiqing Hao, Lusheng Fan, Tong Chen, Ruili Li, Xiaojuan Li, Qihua He, Miguel A. Botella, Jinxing Lin
      Plant Cell Apr 2014, 26 (4) 1729-1745; DOI: https://doi.org/10.1105/tpc.113.122358

      This work used single-particle tracking analysis to detect the dynamics of GFP-RbohD in Arabidopsis. GFP-RbohD spots were found to be mobile with high heterogeneity at the plasma membrane and preferentially assembled into clusters when activated. The results also demonstrated that the internalization of GFP-RbohD occurred via multiple endocytic pathways.

  13. Liu, Chengwu

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  14. Liu, Jinxin

    1. <em>HEAT-INDUCED TAS1 TARGET1</em> Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in <em>Arabidopsis</em>
      You have accessRestricted Access
      HEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in Arabidopsis
      Shuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke He
      Plant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883

      Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.

  15. Liu, Yu

    1. SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Open Access
      SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Qundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping Wu
      Plant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208

      This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.

  16. Liu, Zhongyuan

    1. <em>HEAT-INDUCED TAS1 TARGET1</em> Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in <em>Arabidopsis</em>
      You have accessRestricted Access
      HEAT-INDUCED TAS1 TARGET1 Mediates Thermotolerance via HEAT STRESS TRANSCRIPTION FACTOR A1a–Directed Pathways in Arabidopsis
      Shuxia Li, Jinxin Liu, Zhongyuan Liu, Xiaorong Li, Feijie Wu, Yuke He
      Plant Cell Apr 2014, 26 (4) 1764-1780; DOI: https://doi.org/10.1105/tpc.114.124883

      Heat response pathways in plants are regulated by heat stress transcription factors (Hsfs) and perhaps by small interfering RNAs, such as TAS1-derived siRNAs, but the key mediators of these pathways are unclear. This report identified HEAT-INDUCED TAS1 TARGET1 (HTT1) and HTT2, which are targets of TAS1 and are directly activated by HsfA1a, thus mediating thermotolerance pathways.

  17. Lloyd, Clive W.

    1. Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of <em>Arabidopsis</em> Leaf Trichomes
      You have accessRestricted Access
      Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf Trichomes
      Adrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik Buschmann
      Plant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273

      This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.

  18. López-Igual, Rocío

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

  19. Luginbühl, Leonie

    1. The Enzyme-Like Domain of <em>Arabidopsis</em> Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Open Access
      The Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Sebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. Zeeman
      Plant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703

      This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.

  20. Lv, Qundan

    1. SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Open Access
      SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Qundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping Wu
      Plant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208

      This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.

M

  1. Ma, Xiaonan

    1. A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in <em>Arabidopsis</em>
      You have accessRestricted Access
      A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in Arabidopsis
      Ling Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng Song
      Plant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586

      Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.

  2. Macas, Jiří

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

  3. Mach, Jennifer

    1. Orange Carotenoid Protein Quenches Excess Energy and Singlet Oxygen
      You have accessRestricted Access
      Orange Carotenoid Protein Quenches Excess Energy and Singlet Oxygen
      Jennifer Mach
      Plant Cell Apr 2014, 26 (4) 1380; DOI: https://doi.org/10.1105/tpc.114.126581
  4. Mach, Lukas

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  5. Mao, Chuanzao

    1. SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Open Access
      SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Qundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping Wu
      Plant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208

      This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.

  6. Maresch, Daniel

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  7. Merchant, Sabeeha S.

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  8. Mettler, Tabea

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  9. Miao, Yuchen

    1. A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in <em>Arabidopsis</em>
      You have accessRestricted Access
      A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in Arabidopsis
      Ling Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng Song
      Plant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586

      Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.

  10. Miller, Anthony J.

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  11. Moseley, Jeffrey L.

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  12. Mühlhaus, Timo

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  13. Murray, Jeremy D.

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  14. Mussgnug, Jan H.

    1. Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in <em>Chlamydomonas reinhardtii</em>
      You have accessRestricted Access
      Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii
      Sabrina Grewe, Matteo Ballottari, Marcelo Alcocer, Cosimo D’Andrea, Olga Blifernez-Klassen, Ben Hankamer, Jan H. Mussgnug, Roberto Bassi, Olaf Kruse
      Plant Cell Apr 2014, 26 (4) 1598-1611; DOI: https://doi.org/10.1105/tpc.114.124198

      This work analyzes and elucidates the function of the LHC protein isoform LHCBM9. It provides evidence that the green microalga C. reinhardtii has evolved a LHCII-dependent, medium-term process for photoprotection during environmental stress conditions.

  15. Mysore, Kirankumar S.

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

N

  1. Nagalakshmi, Ugrappa

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  2. Ngo, Kathie J.

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  3. Ning, Kang

    1. Choreography of Transcriptomes and Lipidomes of <em>Nannochloropsis</em> Reveals the Mechanisms of Oil Synthesis in Microalgae
      Open Access
      Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
      Jing Li, Danxiang Han, Dongmei Wang, Kang Ning, Jing Jia, Li Wei, Xiaoyan Jing, Shi Huang, Jie Chen, Yantao Li, Qiang Hu, Jian Xu
      Plant Cell Apr 2014, 26 (4) 1645-1665; DOI: https://doi.org/10.1105/tpc.113.121418

      To reveal the molecular mechanisms underlying lipid accumulation in microalgae, transcriptomic and lipidomic dynamics of Nannochloropsis oceanica under nitrogen-replete and nitrogen-depleted conditions were simultaneously tracked. The temporal and spatial regulation model established in this study provides a basis for the rational genetic engineering of enhanced oil production.

  4. Novák, Petr

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

O

  1. Oldroyd, Giles E.D.

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  2. Oliveira, Ludmila

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

  3. Ott, Thomas

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

P

  1. Palanivelu, Ravishankar

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

  2. Panoli, Aneesh

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

  3. Pant, Bikram Datt

    1. <em>Arabidopsis miR156</em> Regulates Tolerance to Recurring Environmental Stress through <em>SPL</em> Transcription Factors
      You have accessRestricted Access
      Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors
      Anna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel Bäurle
      Plant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851

      The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.

  4. Park, Minho

    1. Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of <em>Arabidopsis</em> PIN-FORMED Proteins
      Open Access
      Functional Analysis of the Hydrophilic Loop in Intracellular Trafficking of Arabidopsis PIN-FORMED Proteins
      Anindya Ganguly, Minho Park, Mahipal Singh Kesawat, Hyung-Taeg Cho
      Plant Cell Apr 2014, 26 (4) 1570-1585; DOI: https://doi.org/10.1105/tpc.113.118422

      The auxin efflux carrier PIN2 has a long hydrophilic loop (HL); when transplanted into PIN5 (a short-HL PIN), this HL confers the PIN5:PIN2-HL fusion protein with the capacity for phosphorylation and plasma membrane (PM) targeting but not for PIN2-like polarity. Moreover, depending on cell type, PIN5 can show internal, PM, or polar localization in the PM.

  5. Patena, Weronika

    1. High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Open Access
      High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
      Ru Zhang, Weronika Patena, Ute Armbruster, Spencer S. Gang, Sean R. Blum, Martin C. Jonikas
      Plant Cell Apr 2014, 26 (4) 1398-1409; DOI: https://doi.org/10.1105/tpc.114.124099

      In this work, the authors develop a method for simultaneously mapping tens of thousands of insertion sites in the green alga Chlamydomonas reinhardtii. This work reveals insights into the mutagenic insertion process and opens the door to pooled enrichment screens and the generation of indexed mutant libraries.

  6. Pellegrini, Matteo

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  7. Perreau, François

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

Q

  1. Qin, Yuan

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

R

  1. Ratet, Pascal

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  2. Ross, John J.

    1. <em>EARLY FLOWERING3</em> Regulates Flowering in Spring Barley by Mediating Gibberellin Production and <em>FLOWERING LOCUS T</em> Expression
      You have accessRestricted Access
      EARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T Expression
      Scott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. Swain
      Plant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794

      This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.

S

  1. Safavian, Darya

    1. The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in <em>Arabidopsis</em>
      You have accessRestricted Access
      The ARC1 E3 Ligase Promotes Two Different Self-Pollen Avoidance Traits in Arabidopsis
      Emily Indriolo, Darya Safavian, Daphne R. Goring
      Plant Cell Apr 2014, 26 (4) 1525-1543; DOI: https://doi.org/10.1105/tpc.114.122879

      This report examines ARC1’s role in reconstituting the self-incompatibility trait in Arabidopsis thaliana and demonstrates an important role for ARC1 in promoting a strong and stable pollen rejection response when expressed with two other A. lyrata self-incompatibility factors. The expression of ARC1 conferred another A. lyrata trait for self-pollen avoidance, termed approach herkogamy.

  2. Salt, David E.

    1. Analysis of the Root System Architecture of <em>Arabidopsis</em> Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Open Access
      Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Fabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna Amtmann
      Plant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101

      Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.

  3. Sambade, Adrian

    1. Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of <em>Arabidopsis</em> Leaf Trichomes
      You have accessRestricted Access
      Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf Trichomes
      Adrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik Buschmann
      Plant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273

      This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.

  4. Schäffner, Anton R.

    1. Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of <em>Arabidopsis</em> Leaf Trichomes
      You have accessRestricted Access
      Actin-Dependent and -Independent Functions of Cortical Microtubules in the Differentiation of Arabidopsis Leaf Trichomes
      Adrian Sambade, Kim Findlay, Anton R. Schäffner, Clive W. Lloyd, Henrik Buschmann
      Plant Cell Apr 2014, 26 (4) 1629-1644; DOI: https://doi.org/10.1105/tpc.113.118273

      This investigation shows that genetic interactions of microtubule function in Arabidopsis trichome differentiation involve ANGUSTIFOLIA1 and F-actin. Detailed analyses of the cytoskeleton of growing trichomes in tortifolia2 distorted1 double mutants indicated that F-actin enhances microtubule dynamics and enables reorientation.

  5. Scheible, Wolf-Rüdiger

    1. <em>Arabidopsis miR156</em> Regulates Tolerance to Recurring Environmental Stress through <em>SPL</em> Transcription Factors
      You have accessRestricted Access
      Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors
      Anna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel Bäurle
      Plant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851

      The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.

  6. Schmollinger, Stefan

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  7. Schoberer, Jennifer

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  8. Schreiber, Lukas

    1. <em>ABORTED MICROSPORES</em> Acts as a Master Regulator of Pollen Wall Formation in <em>Arabidopsis</em>
      Open Access
      ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis
      Jie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing Zhang
      Plant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986

      The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.

  9. Schroda, Michael

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  10. Schultze, Michael

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  11. Seditas, Triona J.

    1. Analysis of the Root System Architecture of <em>Arabidopsis</em> Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Open Access
      Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals
      Fabian Kellermeier, Patrick Armengaud, Triona J. Seditas, John Danku, David E. Salt, Anna Amtmann
      Plant Cell Apr 2014, 26 (4) 1480-1496; DOI: https://doi.org/10.1105/tpc.113.122101

      Root architectures of Arabidopsis thaliana were quantified in multiple combinations of N, P, K, S, and light to understand how plants integrate multiple nutritional signals. Combined with mutant phenotypes, transcriptional profiles, and shoot ion contents, the comprehensive data set facilitates the dissection of the nutrient signaling network and identifies roles for receptor-transporters.

  12. Sedoud, Arezki

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

  13. Shi, Jianxin

    1. <em>ABORTED MICROSPORES</em> Acts as a Master Regulator of Pollen Wall Formation in <em>Arabidopsis</em>
      Open Access
      ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis
      Jie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing Zhang
      Plant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986

      The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.

  14. Shi, Jing

    1. SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Open Access
      SPX4 Negatively Regulates Phosphate Signaling and Homeostasis through Its Interaction with PHR2 in Rice
      Qundan Lv, Yongjia Zhong, Yuguang Wang, Zhiye Wang, Li Zhang, Jing Shi, Zhongchang Wu, Yu Liu, Chuanzao Mao, Keke Yi, Ping Wu
      Plant Cell Apr 2014, 26 (4) 1586-1597; DOI: https://doi.org/10.1105/tpc.114.123208

      This study shows that rice SPX4 negatively regulates phosphate signaling and homeostasis through its interaction with PHR2, a key transcription factor regulating phosphate signaling. SPX4 stabilization is dependent on phosphate concentration and appears to act as a regulatory point both for nuclear localization and for binding of PHR2 to P1BS cis-elements in target DNA.

  15. Siddiqi, Imran

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

  16. Sieh, Daniela

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  17. Šimková, Klára

    1. The Enzyme-Like Domain of <em>Arabidopsis</em> Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Open Access
      The Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Sebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. Zeeman
      Plant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703

      This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.

  18. Skaggs, Megan I.

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

  19. Smith, Steven

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

  20. Sommer, Frederik

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  21. Song, Chun-Peng

    1. A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in <em>Arabidopsis</em>
      You have accessRestricted Access
      A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in Arabidopsis
      Ling Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng Song
      Plant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586

      Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.

  22. Song, Shufei

    1. A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in <em>Arabidopsis</em>
      You have accessRestricted Access
      A Receptor-Like Kinase Mediates Ammonium Homeostasis and Is Important for the Polar Growth of Root Hairs in Arabidopsis
      Ling Bai, Xiaonan Ma, Guozeng Zhang, Shufei Song, Yun Zhou, Lijie Gao, Yuchen Miao, Chun-Peng Song
      Plant Cell Apr 2014, 26 (4) 1497-1511; DOI: https://doi.org/10.1105/tpc.114.124586

      Ammonium (NH4+) is an important nutrient and signal, but can be toxic in excess. Thus, cytoplasmic NH4+ levels must be intricately sensed and tightly controlled. This work identifies a modulator of NH4+ in the tonoplast, CAP1, a receptor-like kinase that mediates NH4+ homeostasis and regulates the polar growth of root hairs by maintaining tip-focused cytoplasmic Ca2+ gradients.

  23. Soyk, Sebastian

    1. The Enzyme-Like Domain of <em>Arabidopsis</em> Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Open Access
      The Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Sebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. Zeeman
      Plant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703

      This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.

  24. Stief, Anna

    1. <em>Arabidopsis miR156</em> Regulates Tolerance to Recurring Environmental Stress through <em>SPL</em> Transcription Factors
      You have accessRestricted Access
      Arabidopsis miR156 Regulates Tolerance to Recurring Environmental Stress through SPL Transcription Factors
      Anna Stief, Simone Altmann, Karen Hoffmann, Bikram Datt Pant, Wolf-Rüdiger Scheible, Isabel Bäurle
      Plant Cell Apr 2014, 26 (4) 1792-1807; DOI: https://doi.org/10.1105/tpc.114.123851

      The authors show that a well-conserved miRNA-transcription factor module implicated previously in developmental control regulates responses to repeated heat stress. They provide a conceptual framework for the integration of environmental stress responses with development to optimize growth under natural conditions.

  25. Stitt, Mark

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  26. Strasser, Richard

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  27. Stratil, Thomas F.

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

  28. Strenkert, Daniela

    1. Nitrogen-Sparing Mechanisms in <em>Chlamydomonas</em> Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      You have accessRestricted Access
      Nitrogen-Sparing Mechanisms in Chlamydomonas Affect the Transcriptome, the Proteome, and Photosynthetic Metabolism
      Stefan Schmollinger, Timo Mühlhaus, Nanette R. Boyle, Ian K. Blaby, David Casero, Tabea Mettler, Jeffrey L. Moseley, Janette Kropat, Frederik Sommer, Daniela Strenkert, Dorothea Hemme, Matteo Pellegrini, Arthur R. Grossman, Mark Stitt, Michael Schroda, Sabeeha S. Merchant
      Plant Cell Apr 2014, 26 (4) 1410-1435; DOI: https://doi.org/10.1105/tpc.113.122523

      This work examines the mechanisms by which Chlamydomonas reinhardtii copes with nitrogen (N) limitation, finding transcriptomic and proteomic changes in multiple metabolic pathways and identifying an N-sparing mechanism that prioritizes respiratory metabolism and shifts the proteomic balance toward proteins with lower N contents, a result with implications for engineering of N-use efficiency.

  29. Suh, Mi Chung

    1. <em>Arabidopsis</em> Cuticular Wax Biosynthesis Is Negatively Regulated by the <em>DEWAX</em> Gene Encoding an AP2/ERF-Type Transcription Factor
      Open Access
      Arabidopsis Cuticular Wax Biosynthesis Is Negatively Regulated by the DEWAX Gene Encoding an AP2/ERF-Type Transcription Factor
      Young Sam Go, Hyojin Kim, Hae Jin Kim, Mi Chung Suh
      Plant Cell Apr 2014, 26 (4) 1666-1680; DOI: https://doi.org/10.1105/tpc.114.123307

      This work identifies a negative transcriptional regulator, DEWAX, that represses the expression of genes involved in Arabidopsis cuticular wax biosynthesis. The results suggest that DEWAX-mediated negative regulation of the wax biosynthetic genes might be involved in determining the total wax loads produced in Arabidopsis during daily dark and light cycles.

  30. Swain, Steve M.

    1. <em>EARLY FLOWERING3</em> Regulates Flowering in Spring Barley by Mediating Gibberellin Production and <em>FLOWERING LOCUS T</em> Expression
      You have accessRestricted Access
      EARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T Expression
      Scott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. Swain
      Plant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794

      This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.

  31. Szymanski, Witold

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

T

  1. Tadege, Million

    1. A H<sup>+</sup>-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and <em>Medicago truncatula</em>
      Open Access
      A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
      Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
      Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: https://doi.org/10.1105/tpc.113.120527

      Electrochemical H+ gradients are essential to drive active transport of solutes through plant membranes. This work describes plant mutants defective in a proton pump that is specifically located in arbuscule-containing root cells and shows that this proton pump is required for the function of the arbuscular mycorrhizal symbiosis and symbiosis-driven phosphate acquisition and plant growth.

  2. Tai, Thomas H.

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  3. Tang, Yuhong

    1. STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in <em>Medicago truncatula</em>
      Open Access
      STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
      Chuanen Zhou, Lu Han, Guifen Li, Maofeng Chai, Chunxiang Fu, Xiaofei Cheng, Jiangqi Wen, Yuhong Tang, Zeng-Yu Wang
      Plant Cell Apr 2014, 26 (4) 1464-1479; DOI: https://doi.org/10.1105/tpc.114.123885

      An analysis of mutants from the compound-leafed model legume Medicago truncatula revealed that the interactions between ARP (PHANTASTICA) and SHOOT MERISTEMLESS (STM)- and BREVIPEDICELLUS (BP)-like KNOXI genes are different from in simple-leafed species, suggesting that the ARP and STM/BP-like KNOXI regulatory module is uncoupled in the formation of compound leaves.

  4. Torres, Giovana A.

    1. Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in <em>Solanum</em> Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      You have accessRestricted Access
      Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres
      Haiqin Zhang, Andrea Koblížková, Kai Wang, Zhiyun Gong, Ludmila Oliveira, Giovana A. Torres, Yufeng Wu, Wenli Zhang, Petr Novák, C. Robin Buell, Jiří Macas, Jiming Jiang
      Plant Cell Apr 2014, 26 (4) 1436-1447; DOI: https://doi.org/10.1105/tpc.114.123877

      This work compares the sequences of five homoeologous centromeres in two closely related species, revealing rapid divergence of centromeric sequences. The results support the idea that centromeric satellite repeats undergo boom-bust cycles before a favorable repeat is fixed in the population.

  5. Trevaskis, Ben

    1. <em>EARLY FLOWERING3</em> Regulates Flowering in Spring Barley by Mediating Gibberellin Production and <em>FLOWERING LOCUS T</em> Expression
      You have accessRestricted Access
      EARLY FLOWERING3 Regulates Flowering in Spring Barley by Mediating Gibberellin Production and FLOWERING LOCUS T Expression
      Scott A. Boden, David Weiss, John J. Ross, Noel W. Davies, Ben Trevaskis, Peter M. Chandler, Steve M. Swain
      Plant Cell Apr 2014, 26 (4) 1557-1569; DOI: https://doi.org/10.1105/tpc.114.123794

      This work shows that the early flowering and growth phenotypes of mutants of barley EARLY FLOWERING3 are promoted by increased production of gibberellin (GA). The authors find that GA is required for flowering and inflorescence development in spring barley grown under long-day conditions and that the GA-dependent control of flowering in barley is independent of FLOWERING LOCUS T1 activity.

  6. Tsukamoto, Tatsuya

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith, Imran Siddiqi, Zhenbiao Yang, Ramin Yadegari, Ravishankar Palanivelu
      Plant Cell Apr 2014, 26 (4) 1612-1628; DOI: https://doi.org/10.1105/tpc.113.120576

      This study shows that ACTIN-RELATED PROTEIN6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development, including expression of the recombinase gene DMC1.

U

  1. Udvardi, Michael

    1. The H<sup>+</sup>-ATPase HA1 of <em>Medicago truncatula</em> Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Open Access
      The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis
      Franziska Krajinski, Pierre-Emmanuel Courty, Daniela Sieh, Philipp Franken, Haoqiang Zhang, Marcel Bucher, Nina Gerlach, Igor Kryvoruchko, Daniela Zoeller, Michael Udvardi, Bettina Hause
      Plant Cell Apr 2014, 26 (4) 1808-1817; DOI: https://doi.org/10.1105/tpc.113.120436

      A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., fungal uptake of phosphate from the soil and release from arbuscules within root cells. This work shows that the M. truncatula proton ATPase HA1 is required for transfer of phosphate across the periarbuscular membrane that separates the fungus from the host plant.

  2. Urbanus, Susan L.

    1. Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in <em>Arabidopsis</em> and <em>Nicotiana benthamiana</em>
      You have accessRestricted Access
      Plasma Membranes Are Subcompartmentalized into a Plethora of Coexisting and Diverse Microdomains in Arabidopsis and Nicotiana benthamiana
      Iris K. Jarsch, Sebastian S.A. Konrad, Thomas F. Stratil, Susan L. Urbanus, Witold Szymanski, Pascal Braun, Karl-Heinz Braun, Thomas Ott
      Plant Cell Apr 2014, 26 (4) 1698-1711; DOI: https://doi.org/10.1105/tpc.114.124446

      The diversity and dynamics of membrane domains in living plant cells is characterized using imaging-based approaches. Examining the diversity of microdomains that are targeted by members of the large Remorin family revealed that these sites may serve as platforms for interactions between different types of membrane-resident proteins and may contribute to their functions.

  3. ur Rehman, Ateeq

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

V

  1. Vasquez-Gross, Hans

    1. Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Open Access
      Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing
      Isabelle M. Henry, Ugrappa Nagalakshmi, Meric C. Lieberman, Kathie J. Ngo, Ksenia V. Krasileva, Hans Vasquez-Gross, Alina Akhunova, Eduard Akhunov, Jorge Dubcovsky, Thomas H. Tai, Luca Comai
      Plant Cell Apr 2014, 26 (4) 1382-1397; DOI: https://doi.org/10.1105/tpc.113.121590

      The authors combined high-throughput global sequencing targeted to genes and custom-designed bioinformatics tools to catalog and characterize chemically induced mutations in rice and wheat. They demonstrate that this approach can be used to develop large-scale induced mutation resources with relatively small investments and is applicable to resource-limited and polyploid organisms.

  2. Vass, Imre

    1. The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      You have accessRestricted Access
      The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher
      Arezki Sedoud, Rocío López-Igual, Ateeq ur Rehman, Adjélé Wilson, François Perreau, Clémence Boulay, Imre Vass, Anja Krieger-Liszkay, Diana Kirilovsky
      Plant Cell Apr 2014, 26 (4) 1781-1791; DOI: https://doi.org/10.1105/tpc.114.123802

      This work shows that the cyanobacterial photoactive orange carotenoid protein (OCP) protects Synechocystis cells from photoinhibition even under conditions in which it is unable to quench excess energy absorbed by phycobilisomes. OCP plays a dual role under light stress conditions, protecting cells against photooxidative stress by quenching excess energy and singlet oxygen.

  3. Vaughan, Cara K.

    1. The Enzyme-Like Domain of <em>Arabidopsis</em> Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Open Access
      The Enzyme-Like Domain of Arabidopsis Nuclear β-Amylases Is Critical for DNA Sequence Recognition and Transcriptional Activation
      Sebastian Soyk, Klára Šimková, Evelyne Zürcher, Leonie Luginbühl, Luise H. Brand, Cara K. Vaughan, Dierk Wanke, Samuel C. Zeeman
      Plant Cell Apr 2014, 26 (4) 1746-1763; DOI: https://doi.org/10.1105/tpc.114.123703

      This study deals with nuclear β-amylase–like proteins, which possess a BZR1-type transcription factor domain and act as transcription factors, showing that their enzymatic-like domain influences DNA binding and ultimately the regulation of gene expression, thereby supporting a role of these proteins in metabolic sensing.

  4. Vavra, Ulrike

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  5. Veit, Christiane

    1. <em>Arabidopsis</em> Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Open Access
      Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum–Associated Degradation of Misfolded Glycoproteins
      Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Eva Liebminger, Daniel Maresch, Josephine Grass, Friedrich Altmann, Lukas Mach, Richard Strasser
      Plant Cell Apr 2014, 26 (4) 1712-1728; DOI: https://doi.org/10.1105/tpc.114.123216

      Protein quality control mechanisms in the endoplasmic reticulum ensure that only correctly folded proteins are delivered to other compartments of the secretory pathwa, while misfolded proteins are degraded. This study identified and characterized the mannosidases that generate the glycan structure acting as the degradation signal for misfolded glycoproteins in Arabidopsis.

  6. Vizcay-Barrena, Gema

    1. <em>ABORTED MICROSPORES</em> Acts as a Master Regulator of Pollen Wall Formation in <em>Arabidopsis</em>
      Open Access
      ABORTED MICROSPORES Acts as a Master Regulator of Pollen Wall Formation in Arabidopsis
      Jie Xu, Zhiwen Ding, Gema Vizcay-Barrena, Jianxin Shi, Wanqi Liang, Zheng Yuan, Danièle Werck-Reichhart, Lukas Schreiber, Zoe A. Wilson, Dabing Zhang
      Plant Cell Apr 2014, 26 (4) 1544-1556; DOI: https://doi.org/10.1105/tpc.114.122986

      The bHLH protein, AMS, acts as a master transcriptional regulator directly modulating the expression of 23 genes related to pollen wall development and sporopollenin biosynthesis in Arabidopsis. These genes are implicated in the separation of microspore mother cells, dissolution of the callose layer of tetrads, and subsequent sporopollenin biosynthesis and pollen coat formation.

W

  1. Wallace, Kirsten N.

    1. ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in <em>Arabidopsis</em>
      You have accessRestricted Access
      ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis
      Yuan Qin, Lihua Zhao, Megan I. Skaggs, Sebastien Andreuzza, Tatsuya Tsukamoto, Aneesh Panoli, Kirsten N. Wallace, Steven Smith,