Skip to main content

Main menu

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in
  • Log out

Search

  • Advanced search
Plant Cell
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Teaching Tools in Plant Biology
    • ASPB
    • Plantae
  • My alerts
  • Log in
  • Log out
Plant Cell

Advanced Search

  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
  • About
    • Editorial Board and Staff
    • About the Journal
    • Terms & Privacy
  • More
    • Alerts
    • Contact Us
  • Submit a Manuscript
    • Instructions for Authors
    • Submit a Manuscript
  • Follow PlantCell on Twitter
  • Visit PlantCell on Facebook
  • Visit Plantae
Research ArticleResearch Article
Open Access

A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula

Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
Ertao Wang
aNational Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: etwang@sibs.ac.cn
Nan Yu
aNational Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S. Asma Bano
cDepartment of Biology, University of York, York YO10 5DD, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chengwu Liu
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anthony J. Miller
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Donna Cousins
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaowei Zhang
aNational Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pascal Ratet
dInstitut des Sciences du Végétale, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Million Tadege
ePlant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kirankumar S. Mysore
ePlant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. Allan Downie
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeremy D. Murray
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Giles E.D. Oldroyd
bJohn Innes Centre, Norwich NR4 7UH, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Schultze
cDepartment of Biology, University of York, York YO10 5DD, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published April 2014. DOI: https://doi.org/10.1105/tpc.113.120527

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • © 2014 American Society of Plant Biologists. All rights reserved.

Abstract

Most plant species form symbioses with arbuscular mycorrhizal (AM) fungi, which facilitate the uptake of mineral nutrients such as phosphate from the soil. Several transporters, particularly proton-coupled phosphate transporters, have been identified on both the plant and fungal membranes and contribute to delivering phosphate from fungi to plants. The mechanism of nutrient exchange has been studied in plants during mycorrhizal colonization, but the source of the electrochemical proton gradient that drives nutrient exchange is not known. Here, we show that plasma membrane H+-ATPases that are specifically induced in arbuscule-containing cells are required for enhanced proton pumping activity in membrane vesicles from AM-colonized roots of rice (Oryza sativa) and Medicago truncatula. Mutation of the H+-ATPases reduced arbuscule size and impaired nutrient uptake by the host plant through the mycorrhizal symbiosis. Overexpression of the H+-ATPase Os-HA1 increased both phosphate uptake and the plasma membrane potential, suggesting that this H+-ATPase plays a key role in energizing the periarbuscular membrane, thereby facilitating nutrient exchange in arbusculated plant cells.

  • Glossary

    AM
    arbuscular mycorrhizal
    • Received November 7, 2013.
    • Revised March 25, 2014.
    • Accepted April 9, 2014.
    • Published April 29, 2014.

    View Full Text
    PreviousNext
    Back to top

    Table of Contents

    Print
    Download PDF
    Email Article

    Thank you for your interest in spreading the word on Plant Cell.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
    (Your Name) has sent you a message from Plant Cell
    (Your Name) thought you would like to see the Plant Cell web site.
    CAPTCHA
    This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
    Citation Tools
    A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
    Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
    The Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: 10.1105/tpc.113.120527

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    Request Permissions
    Share
    A H+-ATPase That Energizes Nutrient Uptake during Mycorrhizal Symbioses in Rice and Medicago truncatula
    Ertao Wang, Nan Yu, S. Asma Bano, Chengwu Liu, Anthony J. Miller, Donna Cousins, Xiaowei Zhang, Pascal Ratet, Million Tadege, Kirankumar S. Mysore, J. Allan Downie, Jeremy D. Murray, Giles E.D. Oldroyd, Michael Schultze
    The Plant Cell Apr 2014, 26 (4) 1818-1830; DOI: 10.1105/tpc.113.120527
    del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
    • Tweet Widget
    • Facebook Like
    • Google Plus One

    Jump to section

    • Article
      • Abstract
      • INTRODUCTION
      • RESULTS
      • DISCUSSION
      • METHODS
      • Acknowledgments
      • AUTHOR CONTRIBUTIONS
      • Footnotes
      • References
    • Figures & Data
    • Info & Metrics
    • PDF

    In this issue

    The Plant Cell Online: 26 (4)
    The Plant Cell
    Vol. 26, Issue 4
    Apr 2014
    • Table of Contents
    • Table of Contents (PDF)
    • About the Cover
    • Index by author
    • Advertising (PDF)
    • Front Matter (PDF)
    View this article with LENS

    More in this TOC Section

    • Ectopic Expression of the Transcriptional Regulator silky3 Causes Pleiotropic Meristem and Sex Determination Defects in Maize Inflorescences
    • SAUR17 and SAUR50 Differentially Regulate PP2C-D1 during Apical Hook Development and Cotyledon Opening in Arabidopsis
    • AUTOPHAGY-RELATED14 and Its Associated Phosphatidylinositol 3-Kinase Complex Promote Autophagy in Arabidopsis
    Show more RESEARCH ARTICLES

    Similar Articles

    Our Content

    • Home
    • Current Issue
    • Plant Cell Preview
    • Archive
    • Teaching Tools in Plant Biology
    • Plant Physiology
    • Plant Direct
    • Plantae
    • ASPB

    For Authors

    • Instructions
    • Submit a Manuscript
    • Editorial Board and Staff
    • Policies
    • Recognizing our Authors

    For Reviewers

    • Instructions
    • Peer Review Reports
    • Journal Miles
    • Transfer of reviews to Plant Direct
    • Policies

    Other Services

    • Permissions
    • Librarian resources
    • Advertise in our journals
    • Alerts
    • RSS Feeds
    • Contact Us

    Copyright © 2021 by The American Society of Plant Biologists

    Powered by HighWire